BumuA.12800.v

Transcriptional regulator, LysR family

CENTER ID: BumuA.12800.v
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.12800.v.A1.GE34678 Full length( BumuA.12800.v ) 1 335
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.5657
RefSeq: YP_001585278.1
UniProt: A9AR20

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRGGNVSSFY LFPSGLESCE TLIHFPRVVN KSPNLEDLRV FSLVVRLASF SAAAEQLAVS PAYVSKRIAM LETQLGTRLL HRSTRRVTVT EAGERVFAHA EKILDDVDHL VEDVSTTRTV PRGTLRISSS FGFGRHVVAP ALLDFSARYP QLNVRLDLFD RLVDVAAEGY DLDVRIGDEI ADHLIARRLA ANYRVLCASP DYLARRGMPR TLADLASHDC LAIKERDHPF GVWRLNVRGD TVTVKVGGAL STNHGEVAVQ WALAGRGIVL RSIWEAGPLI DSGALCRVLP DAVQPANIWA VYTERVAASA KVRVCVDFLA QALAGLNADS AAGAA
NT Sequence
ATGCGCGGCG GCAACGTCTC GTCATTCTAT TTATTTCCAT CCGGATTAGA ATCGTGCGAA ACGTTAATCC ACTTTCCACG AGTCGTGAAC AAATCGCCGA ACCTGGAAGA CCTGCGCGTG TTCAGCCTCG TCGTCCGACT GGCCAGCTTC AGCGCGGCCG CCGAGCAGCT CGCGGTGTCG CCTGCGTACG TGAGCAAGCG CATCGCGATG CTCGAGACGC AGCTCGGCAC GCGCCTGCTG CATCGCTCGA CGCGTCGCGT GACGGTGACC GAAGCGGGCG AGCGCGTGTT CGCGCATGCC GAGAAGATCC TTGACGACGT CGATCATCTC GTCGAAGACG TCTCGACGAC GCGTACGGTA CCGCGCGGCA CGCTGCGCAT TTCGAGCAGT TTCGGTTTCG GCCGGCACGT CGTCGCGCCC GCGCTGCTCG ACTTCAGCGC GCGCTATCCG CAGCTGAACG TGCGGCTCGA TCTGTTCGAC CGGCTCGTCG ACGTGGCGGC CGAGGGATAC GACCTCGACG TGCGGATCGG CGACGAGATC GCCGATCATC TGATCGCGCG TCGCCTCGCC GCGAACTACC GCGTGCTATG TGCGTCGCCC GACTATCTCG CGCGGCGCGG CATGCCGCGC ACGCTCGCGG ATCTCGCGTC GCACGACTGT CTTGCGATCA AGGAGCGCGA TCATCCGTTC GGCGTATGGC GATTGAACGT GCGCGGCGAT ACGGTGACCG TGAAGGTCGG CGGCGCGTTG TCGACGAATC ACGGCGAAGT GGCCGTGCAA TGGGCGCTGG CCGGACGCGG CATCGTGCTG CGCTCGATCT GGGAAGCGGG GCCGCTGATC GACAGCGGCG CGCTGTGCCG CGTGCTGCCC GATGCGGTGC AGCCGGCGAA CATCTGGGCG GTCTATACGG AACGCGTGGC CGCGTCGGCG AAGGTGCGCG TGTGCGTCGA TTTTCTCGCG CAGGCGCTGG CCGGACTCAA TGCCGACTCG GCTGCCGGCG CGGCC
Details for BumuA.12800.v.A1.GE34678
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 80
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRGGNVSSF YLFPSGLESC ETLIHFPRVV NKSPNLEDLR VFSLVVRLAS FSAAAEQLAV SPAYVSKRIA MLETQLGTRL LHRSTRRVTV TEAGERVFAH AEKILDDVDH LVEDVSTTRT VPRGTLRISS SFGFGRHVVA PALLDFSARY PQLNVRLDLF DRLVDVAAEG YDLDVRIGDE IADHLIARRL AANYRVLCAS PDYLARRGMP RTLADLASHD CLAIKERDHP FGVWRLNVRG DTVTVKVGGA LSTNHGEXAV XWALXGRG
Validated NT Sequence
ttaagaagga gntataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgcgcggc ggcaacgtct cgtcattcta tttatttcca tccggattag aatcgtgcga aacgttaatc cactttccac gagtcgtgaa caaatcgccg aacctggaag acctgcgcgt gttcagcctc gtcgtccgac tggccagctt cagcgcggcc gccgagcagc tcgcggtgtc gcctgcgtac gtgagcaagc gcatcgcgat gctcgagacg cagctcggca cgcgcctgct gcatcgctcg acgcgtcgcg tgacggtgac cgaagcgggc gagcgcgtgt tcgcgcatgc cgagaagatc cttgacgacg tcgatcatct cgtcgaagac gtctcgacga cgcgtacggt accgcgcggc acgctgcgca tttcgagcag tttcggtttc ggccggcacg tcgtcgcgcc cgcgctgctc gacttcagcg cgcgctatcc gcagctgaac gtgcggctcg atctgttcga ccggctcgtc gacgtggcgg ccgagggata cgacctcgac gtgcggatcg gcgacgagat cgccgatcat ctgatcgcgc gtcgcctcgc cgcgaactac cgcgtgctat gtgcgtcgcc cgactatctc gcgcggcgcg gcatgccgcg cacgctcgcg gatctcgcgt cgcacgactg tcttgcgatc aaggagcgcg atcatccgtt cggcgtatgg cgattgaacg tgcgcggcga tacngtgacc gtgaaggtcg gcggcgcgtt gtcgacnaat cacggcgaan tggccgtgcn atgggcgctg gncggacgcg gcatc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRGGNVSSF YLFPSGLESC ETLIHFPRVV NKSPNLEDLR VFSLVVRLAS FSAAAEQLAV SPAYVSKRIA MLETQLGTRL LHRSTRRVTV TEAGERVFAH AEKILDDVDH LVEDVSTTRT VPRGTLRISS SFGFGRHVVA PALLDFSARY PQLNVRLDLF DRLVDVAAEG YDLDVRIGDE IADHLIARRL AANYRVLCAS PDYLARRGMP RTLADLASHD CLAIKERDHP FGVWRLNVRG DTVTVKVGGA LSTNHGEVAV QWALAGRGIV LRSIWEAGPL IDSGALCRVL PDAVQPANIW AVYTERVAAS AKVRVCVDFL AQALAGLNAD SAAGAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcggcggc aacgtctcgt cattctattt atttccatcc ggattagaat cgtgcgaaac gttaatccac tttccacgag tcgtgaacaa atcgccgaac ctggaagacc tgcgcgtgtt cagcctcgtc gtccgactgg ccagcttcag cgcggccgcc gagcagctcg cggtgtcgcc tgcgtacgtg agcaagcgca tcgcgatgct cgagacgcag ctcggcacgc gcctgctgca tcgctcgacg cgtcgcgtga cggtgaccga agcgggcgag cgcgtgttcg cgcatgccga gaagatcctt gacgacgtcg atcatctcgt cgaagacgtc tcgacgacgc gtacggtacc gcgcggcacg ctgcgcattt cgagcagttt cggtttcggc cggcacgtcg tcgcgcccgc gctgctcgac ttcagcgcgc gctatccgca gctgaacgtg cggctcgatc tgttcgaccg gctcgtcgac gtggcggccg agggatacga cctcgacgtg cggatcggcg acgagatcgc cgatcatctg atcgcgcgtc gcctcgccgc gaactaccgc gtgctatgtg cgtcgcccga ctatctcgcg cggcgcggca tgccgcgcac gctcgcggat ctcgcgtcgc acgactgtct tgcgatcaag gagcgcgatc atccgttcgg cgtatggcga ttgaacgtgc gcggcgatac ggtgaccgtg aaggtcggcg gcgcgttgtc gacgaatcac ggcgaagtgg ccgtgcaatg ggcgctggcc ggacgcggca tcgtgctgcg ctcgatctgg gaagcggggc cgctgatcga cagcggcgcg ctgtgccgcg tgctgcccga tgcggtgcag ccggcgaaca tctgggcggt ctatacggaa cgcgtggccg cgtcggcgaa ggtgcgcgtg tgcgtcgatt ttctcgcgca ggcgctggcc ggactcaatg ccgactcggc tgccggcgcg gcctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa