BumuA.12800.u

LysR family transcriptional regulator

CENTER ID: BumuA.12800.u
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.12800.u.A1.GE31948 Full length( BumuA.12800.u ) 1 303
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.4329
RefSeq: YP_001584013.1
UniProt: A0A0H3KRU1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDHLQAMRIL ARVAHLGSFT KAAEQLQLPR PTVSNAVQYL EKHLKVRLLQ RTTRRVGLTV EGTTYYERCV RLLADLDDAE TLFEEAGASP RGVVRVDLPE RFALHRVIPA LPDFHARYPD LRVVISMTDR FVDLVADGID CAVRVGTLSD TSLVARRIGE MAQLNCASRD YAARHGLPRT PDELPDHVAV GYFSSRTGRE LDWEYTDMDT GDVHSVKMRS IVSVNSSQAY LACCVAGLGM IQAPRDGLEP LLADGTLVEV LPEWQAPPLP VAVVFPHGRH LAPRVRIFVD WLAETLGDVS RAT
NT Sequence
ATGGATCATT TGCAAGCAAT GCGCATCTTG GCGCGCGTCG CCCATCTCGG CAGCTTCACG AAGGCGGCCG AGCAGCTGCA ACTGCCGCGT CCGACCGTCA GCAACGCGGT CCAGTATCTG GAAAAACATC TGAAGGTACG GCTGCTGCAG CGGACGACGC GTCGCGTCGG GCTGACCGTC GAAGGCACGA CCTACTACGA GCGCTGCGTG CGGCTGCTCG CCGATCTCGA CGACGCGGAA ACGCTGTTCG AAGAGGCCGG CGCGTCGCCG CGCGGCGTCG TTCGCGTCGA TCTGCCCGAG CGTTTCGCGC TGCATCGCGT GATCCCGGCG CTGCCCGATT TCCACGCGCG CTACCCGGAC CTGCGCGTCG TGATCAGCAT GACCGACCGC TTCGTCGACC TCGTCGCGGA CGGCATCGAC TGCGCGGTGC GCGTCGGCAC GCTGTCCGAC ACGTCGCTGG TCGCGCGGCG CATCGGCGAG ATGGCGCAGC TCAACTGCGC GTCGCGCGAC TATGCGGCGC GCCACGGGCT GCCGCGTACG CCCGACGAAC TGCCGGACCA CGTCGCGGTC GGCTATTTCT CGAGCCGCAC GGGCCGCGAA CTGGACTGGG AATACACGGA CATGGACACC GGGGACGTCC ACTCGGTGAA AATGCGCAGC ATCGTGTCGG TGAACAGCTC GCAGGCGTAT CTCGCGTGCT GCGTCGCGGG CCTCGGAATG ATCCAGGCGC CGCGCGACGG CCTCGAGCCG CTGCTCGCGG ACGGCACGCT GGTCGAGGTG CTGCCGGAAT GGCAGGCGCC GCCGCTGCCG GTCGCGGTGG TGTTTCCGCA CGGCCGCCAT CTGGCGCCGC GCGTGCGGAT CTTCGTCGAC TGGCTCGCCG AGACACTCGG CGACGTGAGC CGCGCGACC
Details for BumuA.12800.u.A1.GE31948
HARVESTED ON: 4/5/2011
SEQUENCED ON: 4/11/2011
EXPECTED MW: 35kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMDHLQAM RILARVAHLG SFTKAAEQLQ LPRPTVSNAV QYLEKHLKVR LLQRTTRRVG LTVEGTTYYE RCVRLLADLD DAETLFEEAG ASPRGVVRVD LPERFALHRV IPALPDFHAR YPDLRVVISM TDRFVDLVAD GIDCAVRVGT LSDTSLVARR IGEMAQLNCA SRDYAARHGL PRTPDELPDH VAVGYFSSRT GRELDWEYTD MDTGDVHSVK MRSIVSVNSS QAYLACCVAG LGMIQAPRDG LEPLLADGTL VEVLPEWQAP PLPVAVVFPH GRHLAPRVRI FVDWLAETLG DVSRAT
Validated NT Sequence
ttattaggtc gcgcggctca cgtcgccgag tgtctcggcg agccagtcga cgaagatccg cacgcgcggc gccagatggc ggccgtgcgg aaacaccacc gcgaccggca gcggcggcgc ctgccattcc ggcagcacct cgaccagcgt gccgtccgcg agcagcggct cgaggccgtc gcgcggcgcc tggatcattc cgaggcccgc gacgcagcac gcgagatacg cctgcgagct gttcaccgac acgatgctgc gcattttcac cgagtggacg tccccggtgt ccatgtccgt gtattcccag tccagttcgc ggcccgtgcg gctcgagaaa tagccgaccg cgacgtggtc cggcagttcg tcgggcgtac gcggcagccc gtggcgcgcc gcatagtcgc gcgacgcgca gttgagctgc gccatctcgc cgatgcgccg cgcgaccagc gacgtgtcgg acagcgtgcc gacgcgcacc gcgcagtcga tgccgtccgc gacgaggtcg acgaagcggt cggtcatgct gatcacgacg cgcaggtccg ggtagcgcgc gtggaaatcg ggcagcgccg ggatcacgcg atgcagcgcg aaacgctcgg gcagatcgac gcgaacgacg ccgcgcggcg acgcgccggc ctcttcgaac agcgtttccg cgtcgtcgag atcggcgagc agccgcacgc agcgctcgta gtaggtcgtg ccttcgacgg tcagcccgac gcgacgcgtc gtccgctgca gcagccgtac cttcagatgt ttttccagat actggaccgc gttgctgacg gtcggacgcg gcagttgcag ctgctcggcc gccttcgtga agctgccgag atgggcgacg cgcgccaaga tgcgcattgc ttgcaaatga tccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtganggnnn nnnncc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDHLQAMRI LARVAHLGSF TKAAEQLQLP RPTVSNAVQY LEKHLKVRLL QRTTRRVGLT VEGTTYYERC VRLLADLDDA ETLFEEAGAS PRGVVRVDLP ERFALHRVIP ALPDFHARYP DLRVVISMTD RFVDLVADGI DCAVRVGTLS DTSLVARRIG EMAQLNCASR DYAARHGLPR TPDELPDHVA VGYFSSRTGR ELDWEYTDMD TGDVHSVKMR SIVSVNSSQA YLACCVAGLG MIQAPRDGLE PLLADGTLVE VLPEWQAPPL PVAVVFPHGR HLAPRVRIFV DWLAETLGDV SRAT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatcatttg caagcaatgc gcatcttggc gcgcgtcgcc catctcggca gcttcacgaa ggcggccgag cagctgcaac tgccgcgtcc gaccgtcagc aacgcggtcc agtatctgga aaaacatctg aaggtacggc tgctgcagcg gacgacgcgt cgcgtcgggc tgaccgtcga aggcacgacc tactacgagc gctgcgtgcg gctgctcgcc gatctcgacg acgcggaaac gctgttcgaa gaggccggcg cgtcgccgcg cggcgtcgtt cgcgtcgatc tgcccgagcg tttcgcgctg catcgcgtga tcccggcgct gcccgatttc cacgcgcgct acccggacct gcgcgtcgtg atcagcatga ccgaccgctt cgtcgacctc gtcgcggacg gcatcgactg cgcggtgcgc gtcggcacgc tgtccgacac gtcgctggtc gcgcggcgca tcggcgagat ggcgcagctc aactgcgcgt cgcgcgacta tgcggcgcgc cacgggctgc cgcgtacgcc cgacgaactg ccggaccacg tcgcggtcgg ctatttctcg agccgcacgg gccgcgaact ggactgggaa tacacggaca tggacaccgg ggacgtccac tcggtgaaaa tgcgcagcat cgtgtcggtg aacagctcgc aggcgtatct cgcgtgctgc gtcgcgggcc tcggaatgat ccaggcgccg cgcgacggcc tcgagccgct gctcgcggac ggcacgctgg tcgaggtgct gccggaatgg caggcgccgc cgctgccggt cgcggtggtg tttccgcacg gccgccatct ggcgccgcgc gtgcggatct tcgtcgactg gctcgccgag acactcggcg acgtgagccg cgcgacctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa