SSGCID: Target Page

BumuA.12800.q

LysR family transcriptional regulator

CENTER ID:	BumuA.12800.q
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.12800.q.A1.GE31944	Full length( BumuA.12800.q )		1	306

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.5294
RefSeq:	YP_001584936.1
UniProt:	A0A0H3KK36

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDYIDKLRIF RSVVELRSFT RAADMHGLAR PVVSRAIAEL EARFGGRLLH RTTRQVSLTE TAERIYERCA AVLDELDALE AEAQTQTREP DGLLRLVAHT TAALNQFVPL IAGFKAAHPK VRIDVTLTER PVDLVGEGYD IGIVAPYMLS SDTTIVRLIA RIPFVIVATP AYLRAHGRPA TPADLVDHPF VPLSPSLRRP ALSFRVDGDV LTLPLRFDIS SNSPVFNREM VMHGFGIGIV PRTLIETELA SGALVQLLGD ADLVDGCVEL KLAYANRSLL PAKVKAFIDY ASAMCDREGW ITPVAA

NT Sequence

ATGGACTACA TCGACAAGCT GCGGATCTTC CGGTCGGTCG TCGAGCTGCG CAGCTTCACG CGCGCGGCCG ACATGCATGG CCTCGCGCGT CCGGTCGTGT CGCGCGCGAT CGCCGAACTC GAAGCGCGGT TCGGCGGCCG CTTGCTGCAC CGGACCACGC GCCAGGTGTC GCTGACCGAA ACGGCCGAAC GCATCTACGA GCGCTGCGCG GCCGTGCTCG ACGAGCTGGA CGCGCTCGAA GCCGAAGCGC AGACGCAGAC GCGCGAGCCC GACGGCCTGC TGCGGCTCGT CGCGCACACG ACCGCCGCGC TGAATCAGTT CGTGCCGTTG ATCGCGGGCT TCAAGGCTGC GCATCCGAAA GTGCGTATCG ACGTCACGTT GACCGAGCGG CCCGTCGATC TGGTCGGCGA GGGCTACGAC ATCGGCATCG TCGCTCCCTA CATGCTGAGC TCCGACACGA CGATCGTGCG ACTCATCGCG CGGATACCGT TCGTGATCGT GGCGACGCCC GCGTACCTGC GCGCGCATGG GCGCCCCGCG ACGCCGGCCG ATCTCGTCGA CCATCCGTTC GTGCCGCTGT CGCCGTCGCT GCGCCGCCCG GCGCTGTCGT TCCGCGTCGA CGGCGACGTG CTGACCCTTC CGCTGCGCTT CGACATTTCG TCGAACAGCC CCGTGTTCAA CCGCGAGATG GTGATGCACG GTTTCGGCAT CGGCATCGTG CCGAGAACGC TCATTGAGAC CGAGCTCGCG TCGGGCGCGC TCGTGCAGCT GCTCGGCGAC GCCGATCTCG TCGACGGGTG CGTCGAGCTG AAGCTCGCGT ACGCGAACCG GTCGCTGCTG CCGGCGAAGG TCAAGGCGTT CATCGACTAT GCGAGCGCGA TGTGCGACCG CGAAGGCTGG ATCACGCCCG TCGCGGCA

Details for BumuA.12800.q.A1.GE31944

HARVESTED ON:	4/5/2011
SEQUENCED ON:	4/11/2011
EXPECTED MW:	35kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

atgccgcgac gggcgtgatc cagccttcgc ggtcgcacat cgcgctcgca tagtcgatga acgccttgac cttcgccggc agcagcgacc ggttcgcgta cgcgagcttc agctcgacgc acccgtcgac gagatcggcg tcgccgagca gctgcacgag cgcgcccgac gcgagctcgg tctcaatgag cgttctcggc acgatgccga tgccgaaacc gtgcatcacc atctcgcggt tgaacacggg gctgttcgac gaaatgtcga agcgcagcgg aagggtcagc acgtcgccgt cgacgcggaa cgacagcgcc gggcggcgca gcgacggcga cagcggcacg aacggatggt cgacgagatc ggccggcgtc gcggggcgcc catgcgcgcg caggtacgcg ggcgtcgcca cgatcacgaa cggtatccgc gcgatgagtc gcacgatcgt cgtgtcggag ctcagcatgt agggagcgac gatgccgatg tcgtagccct cgccgaccag atcgacgggc cgctcggtca acgtgacgtc gatacgcact ttcggatgcg cagccttgaa gcccgcgatc aacggcacga actgattcag cgcggcggtc gtgtgcgcga cgagccgcag caggccgtcg ggctcgcgcg tctgcgtctg cgcttcggct tcgagcgcgt ccagctcgtc gagcacggcc gcgcagcgct cgtagatgcg ttcggccgtt tcggtcagcg acacctggcg cgtggtccgg tgcagcaagc ggccgccgaa ccgcgcttcg agttcggcga tcgcgcgcga cacgaccgga cgcgcgaggc catgcatgtc ggccgcgcgc gtgaagctgc gcagctcgac gaccgaccgg aagatccgca gcttgtcgat gtagtccatc gaaccaggac cc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDYIDKLRI FRSVVELRSF TRAADMHGLA RPVVSRAIAE LEARFGGRLL HRTTRQVSLT ETAERIYERC AAVLDELDAL EAEAQTQTRE PDGLLRLVAH TTAALNQFVP LIAGFKAAHP KVRIDVTLTE RPVDLVGEGY DIGIVAPYML SSDTTIVRLI ARIPFVIVAT PAYLRAHGRP ATPADLVDHP FVPLSPSLRR PALSFRVDGD VLTLPLRFDI SSNSPVFNRE MVMHGFGIGI VPRTLIETEL ASGALVQLLG DADLVDGCVE LKLAYANRSL LPAKVKAFID YASAMCDREG WITPVAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggactacatc gacaagctgc ggatcttccg gtcggtcgtc gagctgcgca gcttcacgcg cgcggccgac atgcatggcc tcgcgcgtcc ggtcgtgtcg cgcgcgatcg ccgaactcga agcgcggttc ggcggccgct tgctgcaccg gaccacgcgc caggtgtcgc tgaccgaaac ggccgaacgc atctacgagc gctgcgcggc cgtgctcgac gagctggacg cgctcgaagc cgaagcgcag acgcagacgc gcgagcccga cggcctgctg cggctcgtcg cgcacacgac cgccgcgctg aatcagttcg tgccgttgat cgcgggcttc aaggctgcgc atccgaaagt gcgtatcgac gtcacgttga ccgagcggcc cgtcgatctg gtcggcgagg gctacgacat cggcatcgtc gctccctaca tgctgagctc cgacacgacg atcgtgcgac tcatcgcgcg gataccgttc gtgatcgtgg cgacgcccgc gtacctgcgc gcgcatgggc gccccgcgac gccggccgat ctcgtcgacc atccgttcgt gccgctgtcg ccgtcgctgc gccgcccggc gctgtcgttc cgcgtcgacg gcgacgtgct gacccttccg ctgcgcttcg acatttcgtc gaacagcccc gtgttcaacc gcgagatggt gatgcacggt ttcggcatcg gcatcgtgcc gagaacgctc attgagaccg agctcgcgtc gggcgcgctc gtgcagctgc tcggcgacgc cgatctcgtc gacgggtgcg tcgagctgaa gctcgcgtac gcgaaccggt cgctgctgcc ggcgaaggtc aaggcgttca tcgactatgc gagcgcgatg tgcgaccgcg aaggctggat cacgcccgtc gcggcataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa