BumuA.12800.o

LysR family transcriptional regulator

CENTER ID: BumuA.12800.o
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.12800.o.A2.GE34667 Mature protein( BumuA.12800.o ) 27 304
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.3667
RefSeq: YP_001583379.1
UniProt: A0A0H3KTJ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRSSSEKLSG SISVFAAVVD AGTFVGAAEV IGMTPPGVSR AIARLEKRLN VRLFNRTTRS VSLTEEGRRF YEQVMPHLAG LEEAAAAAAG GASAVRGKLR INLDPVVYRT VLGQPLDAFM DTHPELEIEF IARDSLGDLV MDGFDLAVRF GEPKASTLIA RKLLDSRIVT VAAPSYVARW GRPAKPEDLE GRRHRCLEFR NPETGKPFQW EFHRKRKRIV VNTQGRLTVN DPGALLNACL AGSGIAQMLL LGAEPLIADG RLINLFPEWV DERYPLYVYY ASRHHVPAKT RAFLDFVVEL MRNH
NT Sequence
ATGAGATCCA GTAGCGAGAA ACTTTCCGGC AGCATTAGCG TGTTCGCCGC CGTAGTGGAT GCGGGGACCT TTGTGGGCGC TGCGGAGGTG ATAGGAATGA CGCCGCCGGG CGTCAGTCGC GCGATTGCGC GGCTGGAAAA GAGGTTAAAC GTCCGGCTCT TCAACCGGAC AACGCGCTCT GTCTCCCTGA CTGAAGAGGG CCGCCGCTTT TACGAGCAGG TCATGCCCCA TCTGGCCGGC CTTGAGGAAG CGGCGGCCGC TGCGGCGGGT GGCGCGTCCG CTGTGCGTGG AAAGCTGAGA ATCAATCTGG ACCCTGTCGT CTACCGGACC GTTCTCGGGC AGCCACTCGA TGCGTTCATG GACACACACC CGGAACTCGA AATCGAGTTC ATCGCGCGGG ACAGTCTTGG CGACCTCGTC ATGGACGGCT TCGACCTCGC CGTCCGATTC GGCGAACCCA AAGCCTCCAC GCTGATCGCA AGAAAGCTGC TCGACTCCCG CATCGTGACA GTTGCAGCAC CGTCGTACGT TGCACGTTGG GGACGGCCGG CAAAACCCGA GGACCTGGAA GGTCGACGCC ACAGATGCCT CGAATTTCGA AACCCGGAGA CTGGCAAGCC GTTCCAGTGG GAATTCCATC GCAAACGAAA ACGCATTGTG GTGAACACTC AGGGACGACT CACGGTCAAC GACCCGGGCG CTCTGCTTAA CGCATGTCTC GCCGGTTCCG GGATTGCGCA GATGTTGCTG CTCGGTGCCG AGCCCCTGAT TGCCGACGGG CGGTTGATCA ATCTCTTTCC CGAATGGGTA GACGAACGGT ATCCGTTATA CGTCTACTAC GCATCCCGGC ACCACGTGCC GGCAAAGACC CGCGCTTTTC TGGACTTCGT GGTGGAACTG ATGCGCAATC AC
Details for BumuA.12800.o.A2.GE34667
HARVESTED ON: 1/31/2012
SEQUENCED ON: 2/10/2012
EXPECTED MW: 33kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMAAEVIG MTPPGVSRAI ARLEKRLNVR LFNRTTRSVS LTEEGRRFYE QVMPHLAGLE EAAAAAAGGA SAVRGKLRIN LDPVVYRTVL GQPLDAFMDT HPELEIEFIA RDSLGDLVMD GFDLAVRFGE PKASTLIARK LLDSRIVTVA APSYVARWGR PAKPEDLEGR RHRCLEFRNP ETGKPFQWEF HRKRKRIVVN TQGRLTVNDP GALLNACLAG SGIAQMLLLG AEPLIADGRL INLFPEWVDE RYPLYVYYAS RHHVPAKTRA FLDFVVELMR
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggctgcg gaggtgatag gaatgacgcc gccgggcgtc agtcgcgcga ttgcgcggct ggaaaagagg ttaaacgtcc ggctcttcaa ccggacaacg cgctctgtct ccctgactga agagggccgc cgcttttacg agcaggtcat gccccatctg gccggccttg aggaagcggc ggccgctgcg gcgggtggcg cgtccgctgt gcgtggaaag ctgagaatca atctggaccc tgtcgtctac cggaccgttc tcgggcagcc actcgatgcg ttcatggaca cacacccgga actcgaaatc gagttcatcg cgcgggacag tcttggcgac ctcgtcatgg acggcttcga cctcgccgtc cgattcggcg aacccaaagc ctccacgctg atcgcaagaa agctgctcga ctcccgcatc gtgacagttg cagcaccgtc gtacgttgca cgttggggac ggccggcaaa acccgaggac ctggaaggtc gacgccacag atgcctcgaa tttcgaaacc cggagactgg caagccgttc cagtgggaat tccatcgcaa acgaaaacgc attgtggtga acactcaggg acgactcacg gtcaacgacc cgggcgctct gcttaacgca tgtctcgccg gttccgggat tgcgcagatg ttgctgctcg gtgccgagcc cctgattgcc gacgggcggt tgatcaatct ctttcccgaa tgggtagacg aacggtatcc gttatacgtc tactacgcat cccggcacca cgtgccggca aagacccgcg cttttctgga cttcgtggtg gaactgatgc gca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAEVIGMT PPGVSRAIAR LEKRLNVRLF NRTTRSVSLT EEGRRFYEQV MPHLAGLEEA AAAAAGGASA VRGKLRINLD PVVYRTVLGQ PLDAFMDTHP ELEIEFIARD SLGDLVMDGF DLAVRFGEPK ASTLIARKLL DSRIVTVAAP SYVARWGRPA KPEDLEGRRH RCLEFRNPET GKPFQWEFHR KRKRIVVNTQ GRLTVNDPGA LLNACLAGSG IAQMLLLGAE PLIADGRLIN LFPEWVDERY PLYVYYASRH HVPAKTRAFL DFVVELMRNH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgcggag gtgataggaa tgacgccgcc gggcgtcagt cgcgcgattg cgcggctgga aaagaggtta aacgtccggc tcttcaaccg gacaacgcgc tctgtctccc tgactgaaga gggccgccgc ttttacgagc aggtcatgcc ccatctggcc ggccttgagg aagcggcggc cgctgcggcg ggtggcgcgt ccgctgtgcg tggaaagctg agaatcaatc tggaccctgt cgtctaccgg accgttctcg ggcagccact cgatgcgttc atggacacac acccggaact cgaaatcgag ttcatcgcgc gggacagtct tggcgacctc gtcatggacg gcttcgacct cgccgtccga ttcggcgaac ccaaagcctc cacgctgatc gcaagaaagc tgctcgactc ccgcatcgtg acagttgcag caccgtcgta cgttgcacgt tggggacggc cggcaaaacc cgaggacctg gaaggtcgac gccacagatg cctcgaattt cgaaacccgg agactggcaa gccgttccag tgggaattcc atcgcaaacg aaaacgcatt gtggtgaaca ctcagggacg actcacggtc aacgacccgg gcgctctgct taacgcatgt ctcgccggtt ccgggattgc gcagatgttg ctgctcggtg ccgagcccct gattgccgac gggcggttga tcaatctctt tcccgaatgg gtagacgaac ggtatccgtt atacgtctac tacgcatccc ggcaccacgt gccggcaaag acccgcgctt ttctggactt cgtggtggaa ctgatgcgca atcactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa