SSGCID: Target Page

BumuA.12800.m

LysR family transcriptional regulator

CENTER ID:	BumuA.12800.m
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.12800.m.A1.GE34674	Full length( BumuA.12800.m )		1	320

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.3194
RefSeq:	YP_001581143.1
UniProt:	A0A0H3KK20

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDTLVSMNVF RYVVEVGSFV GAAERMQMSA AMASKHVMHL EQQLGARLLH RTTRRVAPTE AGREYYERLV QALSELDEAG QAVGAASVVP QGRLRVTSLS AFGLRHVMQA VTDYAERFPD VTVEITLSDR VVELIDEGYD VAVRAAPSGL KSSSLVARQI ATAHILLVAS PEYLKKHGTP ETLADLAQHN YLRRDSNAST LDAMLNDAAA ASRVALNGNL IVNHLEGLRA AVLAGAGIAL LGTEVVGDDI EAGRLVPVLV DAVPPRELPI YAVYASRRHV SAKVRSFVDF LAARFEGQSL CPSIESRSHV LPMSRMKRTG

NT Sequence

ATGGATACCC TCGTCAGCAT GAATGTGTTT CGCTACGTCG TCGAAGTGGG CAGCTTCGTC GGCGCGGCGG AGCGCATGCA AATGTCGGCG GCGATGGCGA GCAAGCACGT GATGCACCTG GAGCAGCAGC TCGGCGCACG GCTGCTGCAT CGTACGACGC GACGCGTCGC GCCCACCGAG GCGGGACGCG AATATTACGA GCGCCTCGTG CAGGCGCTGT CCGAACTCGA CGAAGCGGGA CAGGCCGTCG GCGCCGCGAG CGTGGTGCCG CAAGGGCGGC TGCGCGTGAC GTCGCTGTCC GCATTCGGCC TGCGTCACGT GATGCAGGCC GTCACCGATT ATGCGGAGCG CTTTCCGGAC GTGACGGTCG AGATCACGCT GTCCGATCGT GTCGTCGAAC TGATCGACGA GGGCTACGAC GTCGCCGTGC GCGCGGCGCC GAGCGGACTG AAGTCGTCGT CGCTCGTGGC GCGCCAGATC GCGACCGCGC ACATTCTGCT CGTCGCGTCG CCCGAATATC TGAAAAAGCA CGGGACGCCC GAGACGCTCG CGGATCTCGC GCAGCACAAC TATCTGCGGC GCGACTCGAA CGCGTCGACG CTCGACGCGA TGCTGAACGA CGCGGCCGCC GCGTCGCGCG TGGCGCTGAA CGGCAATCTG ATCGTCAACC ATCTCGAAGG CCTGCGTGCG GCCGTGCTGG CCGGCGCCGG CATTGCGCTG CTCGGCACCG AAGTGGTCGG CGACGACATC GAGGCGGGGC GCCTCGTGCC CGTGCTCGTC GATGCGGTGC CGCCGCGCGA GCTGCCGATC TATGCGGTGT ATGCGAGCCG TCGCCACGTG TCGGCGAAGG TGCGCTCGTT CGTCGATTTT CTCGCTGCGC GCTTCGAAGG GCAGTCGCTG TGTCCGTCGA TCGAATCGCG CTCGCACGTG CTGCCGATGT CGCGGATGAA GCGCACGGGC

Details for BumuA.12800.m.A1.GE34674

HARVESTED ON:	1/31/2012
SEQUENCED ON:	3/16/2012
EXPECTED MW:	36kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Validated AA Sequence

MAHHHHHHMG XXEAQTQGPG SMDXLVSMNV FRYVVEVGSF VGAAERMQMS AAMASKHVMH LEQQLGARLL HRTTRRVAPT EAGREYYERL VQALSELDEA GQAVGAASVV PQGRLRVTSL SAFGLRHVMQ AVTDYAERFP DVTVEITLSD RVVELIDEGY DVAVRAAPSG LKSSSLVARQ IATAHILLVA SPEYLKKHGT PETLADLAQH NYLRRDSNAS TLDAMLNDAA AASRVALNGN LIVNHLEGLR AAVLAGAGIA LLGTEVVGDD IEAGRLVPVL VDAVPPRELP IYAVYASRRH VSAKVRSFVD FLAARFEGQ

Validated NT Sequence

ataccatggc tcatcaccat caccatcata tgggtncnnn ggaagctcag acccagggtc ctggttcgat ggatnccctc gtcagcatga atgtgtttcg ctacgtcgtc gaagtgggca gcttcgtcgg cgcggcggag cgcatgcaaa tgtcggcggc gatggcgagc aagcacgtga tgcacctgga gcagcagctc ggcgcacggc tgctgcatcg tacgacgcga cgcgtcgcgc ccaccgaggc gggacgcgaa tattacgagc gcctcgtgca ggcgctgtcc gaactcgacg aagcgggaca ggccgtcggc gccgcgagcg tggtgccgca agggcggctg cgcgtgacgt cgctgtccgc attcggcctg cgtcacgtga tgcaggccgt caccgattat gcggagcgct ttccggacgt gacggtcgag atcacgctgt ccgatcgtgt cgtcgaactg atcgacgagg gctacgacgt cgccgtgcgc gcggcgccga gcggactgaa gtcgtcgtcg ctcgtggcgc gccagatcgc gaccgcgcac attctgctcg tcgcgtcgcc cgaatatctg aaaaagcacg ggacgcccga gacgctcgcg gatctcgcgc agcacaacta tctgcggcgc gactcgaacg cgtcgacgct cgacgcgatg ctgaacgacg cggccgccgc gtcgcgcgtg gcgctgaacg gcaatctgat cgtcaaccat ctcgaaggcc tgcgtgcggc cgtgctggcc ggcgccggca ttgcgctgct cggcaccgaa gtggtcggcg acgacatcga ggcggggcgc ctcgtgcccg tgctcgtcga tgcggtgccg ccgcgcgagc tgccgatcta tgcggtgtat gcgagccgtc gccacgtgtc ggcgaaggtg cgctcgttcg tcgattttct cgctgcgcgc ttcgaagggc agtcg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDTLVSMNV FRYVVEVGSF VGAAERMQMS AAMASKHVMH LEQQLGARLL HRTTRRVAPT EAGREYYERL VQALSELDEA GQAVGAASVV PQGRLRVTSL SAFGLRHVMQ AVTDYAERFP DVTVEITLSD RVVELIDEGY DVAVRAAPSG LKSSSLVARQ IATAHILLVA SPEYLKKHGT PETLADLAQH NYLRRDSNAS TLDAMLNDAA AASRVALNGN LIVNHLEGLR AAVLAGAGIA LLGTEVVGDD IEAGRLVPVL VDAVPPRELP IYAVYASRRH VSAKVRSFVD FLAARFEGQS LCPSIESRSH VLPMSRMKRT G

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggataccctc gtcagcatga atgtgtttcg ctacgtcgtc gaagtgggca gcttcgtcgg cgcggcggag cgcatgcaaa tgtcggcggc gatggcgagc aagcacgtga tgcacctgga gcagcagctc ggcgcacggc tgctgcatcg tacgacgcga cgcgtcgcgc ccaccgaggc gggacgcgaa tattacgagc gcctcgtgca ggcgctgtcc gaactcgacg aagcgggaca ggccgtcggc gccgcgagcg tggtgccgca agggcggctg cgcgtgacgt cgctgtccgc attcggcctg cgtcacgtga tgcaggccgt caccgattat gcggagcgct ttccggacgt gacggtcgag atcacgctgt ccgatcgtgt cgtcgaactg atcgacgagg gctacgacgt cgccgtgcgc gcggcgccga gcggactgaa gtcgtcgtcg ctcgtggcgc gccagatcgc gaccgcgcac attctgctcg tcgcgtcgcc cgaatatctg aaaaagcacg ggacgcccga gacgctcgcg gatctcgcgc agcacaacta tctgcggcgc gactcgaacg cgtcgacgct cgacgcgatg ctgaacgacg cggccgccgc gtcgcgcgtg gcgctgaacg gcaatctgat cgtcaaccat ctcgaaggcc tgcgtgcggc cgtgctggcc ggcgccggca ttgcgctgct cggcaccgaa gtggtcggcg acgacatcga ggcggggcgc ctcgtgcccg tgctcgtcga tgcggtgccg ccgcgcgagc tgccgatcta tgcggtgtat gcgagccgtc gccacgtgtc ggcgaaggtg cgctcgttcg tcgattttct cgctgcgcgc ttcgaagggc agtcgctgtg tccgtcgatc gaatcgcgct cgcacgtgct gccgatgtcg cggatgaagc gcacgggcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a