SSGCID: Target Page

BumuA.12800.k

LysR family transcriptional regulator

CENTER ID:	BumuA.12800.k
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.12800.k.A2.GE31938	Mature protein( BumuA.12800.k )		25	301

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.1406
RefSeq:	YP_001579412.1
UniProt:	A0A0H3KKI2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRFKQIETF VAVAAKGSLS AAAHAEGVAP AIIGRRLDAL EERLGVKLLV RTTRKLTLTF EGSAFLEDCQ RIINDMQNAE ASVSAGGVKA SGHLRITAPA GFGRRHVAPL VPDFSGAHPD VSVTLDLSDR MVDLVNEGFD CAVRLGELPD SSLVSLKLGE NRRVCVASPA YLARRGTPTT LAELARHNCL ALAANANQQR GWTFQEDDKV VSIRVSGTME CSDGAVLHEW CLAGHGLAWR SWWEVGDDIA AGRLVSVLDA FAAPPIGIHA VFPQRRHLPL RVRLFLDYLK HTYERPGYWG E

NT Sequence

ATGGACCGGT TCAAACAGAT CGAAACGTTC GTCGCGGTCG CGGCGAAAGG CAGCCTGTCG GCGGCCGCGC ACGCGGAGGG CGTCGCGCCG GCGATCATCG GCCGCCGTCT CGACGCGCTC GAGGAGCGGC TCGGCGTCAA GCTGCTCGTG CGCACGACGC GCAAGCTCAC GCTGACCTTC GAGGGCTCCG CATTCCTCGA GGATTGCCAG CGCATCATCA ACGACATGCA GAACGCGGAA GCGAGCGTGT CCGCCGGCGG CGTGAAGGCG AGCGGCCATT TGCGCATCAC GGCGCCCGCC GGCTTCGGCC GCCGCCACGT CGCGCCGCTC GTGCCCGACT TCAGCGGCGC GCATCCGGAC GTGTCGGTCA CGCTCGATCT GTCGGACCGG ATGGTCGACC TCGTCAACGA AGGCTTCGAT TGCGCGGTGC GGCTCGGCGA GCTGCCCGAT TCGTCGCTCG TGTCGCTGAA GCTCGGCGAG AACCGGCGCG TGTGCGTCGC GTCGCCCGCG TATCTCGCGC GGCGCGGCAC GCCGACGACG CTCGCGGAAC TCGCGCGCCA CAACTGCCTC GCGCTCGCCG CGAACGCGAA CCAGCAGCGC GGCTGGACAT TCCAGGAAGA CGACAAGGTC GTGTCGATCC GCGTGAGCGG CACGATGGAG TGCTCCGACG GCGCGGTGCT GCACGAGTGG TGCCTGGCCG GCCACGGTCT GGCATGGCGA TCGTGGTGGG AAGTCGGCGA CGACATCGCG GCCGGCCGCC TCGTCAGCGT GCTGGACGCG TTCGCGGCGC CGCCGATCGG CATTCATGCC GTATTTCCGC AGCGCCGGCA CCTGCCGCTG CGCGTGCGGC TGTTTCTCGA TTACCTGAAG CACACGTACG AGCGGCCCGG TTACTGGGGC GAA

Details for BumuA.12800.k.A2.GE31938

HARVESTED ON:	4/5/2011
SEQUENCED ON:	4/11/2011
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	96
PERCENT COVERAGE:	111

Validated AA Sequence

MAHXHHHHMG TLEAQTQGPG SMAEGVAPAI IGRRLDALEE RLGVKLLVRT TRKLTLTFEG SAFLEDCQRI INDMQNAEAS VSAGGVKASG HLRITAPAGF GRRHVAPLVP DFSGAHPDVS VTLDLSDRMV DLVNEGFDCA VRLGELPDSS LVSLKLGENR RVCVASPAYL ARRGTPTTLA ELARHNCLAL AANANQQRGW TFQEDDKVVS IRVSGTMECS DGAVLHEWCL XGHGLAWRSW WEVGDDIAAG RLVSVLXAFA APPIGIHAVX PQRXHLXLRV RLXLDXXKHT YERXGYWGNN KXHEXVXQPS FSRXRLLXXX XXKLXXAXXX XXX

Validated NT Sequence

gnanngnnta ccatggctca tcnccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggc ggagggcgtc gcgccggcga tcatcggccg ccgtctcgac gcgctcgagg agcggctcgg cgtcaagctg ctcgtgcgca cgacgcgcaa gctcacgctg accttcgagg gctccgcatt cctcgaggat tgccagcgca tcatcaacga catgcagaac gcggaagcga gcgtgtccgc cggcggcgtg aaggcgagcg gccatttgcg catcacggcg cccgccggct tcggccgccg ccacgtcgcg ccgctcgtgc ccgacttcag cggcgcgcat ccggacgtgt cggtcacgct cgatctgtcg gaccggatgg tcgacctcgt caacgaaggc ttcgattgcg cggtgcggct cggcgagctg cccgattcgt cgctcgtgtc gctgaagctc ggcgagaacc ggcgcgtgtg cgtcgcgtcg cccgcgtatc tcgcgcggcg cggcacgccg acgacgctcg cggaactcgc gcgccacaac tgcctcgcgc tcgccgcgaa cgcgaaccag cagcgcggct ggacattcca ggaagacgac aaggtcgtgt cgatccgcgt gagcggcacg atggagtgct ccgacggcgc ggtgctgcac gagtggtgcc tggncggcca cggtctggca tggcgatcgt ggtgggaagt cggcgacgac atcgcggccg gccgcctcgt cagcgtgctg gangcgttcg cggcgccgcc gatcggcatt catgccgtan ttccgcagcg ccngcacctg ncgctgcgcg tgcggctgnt tctcgattan nngaagcaca cgtacgagcg gnnnggttac tgggggaata ataaacanca cgaacnagtt ntgcagccaa gcttctcnag gnnncggctg ctancaaann cnnnanngaa gctgnnnnnn gcnngctnnc nnncntgnnn nnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAEGVAPAI IGRRLDALEE RLGVKLLVRT TRKLTLTFEG SAFLEDCQRI INDMQNAEAS VSAGGVKASG HLRITAPAGF GRRHVAPLVP DFSGAHPDVS VTLDLSDRMV DLVNEGFDCA VRLGELPDSS LVSLKLGENR RVCVASPAYL ARRGTPTTLA ELARHNCLAL AANANQQRGW TFQEDDKVVS IRVSGTMECS DGAVLHEWCL AGHGLAWRSW WEVGDDIAAG RLVSVLDAFA APPIGIHAVF PQRRHLPLRV RLFLDYLKHT YERPGYWGE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggagggc gtcgcgccgg cgatcatcgg ccgccgtctc gacgcgctcg aggagcggct cggcgtcaag ctgctcgtgc gcacgacgcg caagctcacg ctgaccttcg agggctccgc attcctcgag gattgccagc gcatcatcaa cgacatgcag aacgcggaag cgagcgtgtc cgccggcggc gtgaaggcga gcggccattt gcgcatcacg gcgcccgccg gcttcggccg ccgccacgtc gcgccgctcg tgcccgactt cagcggcgcg catccggacg tgtcggtcac gctcgatctg tcggaccgga tggtcgacct cgtcaacgaa ggcttcgatt gcgcggtgcg gctcggcgag ctgcccgatt cgtcgctcgt gtcgctgaag ctcggcgaga accggcgcgt gtgcgtcgcg tcgcccgcgt atctcgcgcg gcgcggcacg ccgacgacgc tcgcggaact cgcgcgccac aactgcctcg cgctcgccgc gaacgcgaac cagcagcgcg gctggacatt ccaggaagac gacaaggtcg tgtcgatccg cgtgagcggc acgatggagt gctccgacgg cgcggtgctg cacgagtggt gcctggccgg ccacggtctg gcatggcgat cgtggtggga agtcggcgac gacatcgcgg ccggccgcct cgtcagcgtg ctggacgcgt tcgcggcgcc gccgatcggc attcatgccg tatttccgca gcgccggcac ctgccgctgc gcgtgcggct gtttctcgat tacctgaagc acacgtacga gcggcccggt tactggggcg aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa