BumuA.12800.i

LysR family transcriptional regulator

CENTER ID: BumuA.12800.i
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.12800.i.A1.GE34671 Full length( BumuA.12800.i ) 1 313
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.1694
RefSeq: YP_001579678.1
UniProt: A0A0H3KEZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKADPIPAPD KPLDLLDVGL FVRAALLANV TAAGREFGVS AAVASARIAQ LERQLGARLL HRTTRRISLT QDGEIFMARA EALLSAADAA RASVGRGRTE PYGRLKVSMS SSFGRQHVTP VIPAFLRRYP SVSLDLRLSD EIVDLVDEGY DVAIRLGALK DSTLVARKLA TNRRVLCCSP AYLAERGTPR HPADLAQHEC VILGDQRDWS FATPQGPFTV RVGGRLVASN GEAIREALVD GFGIAIKSTW DVGPLLASGA LVTVLDDYPF AEDIAVWAVY PSRAHVPLKT LAFIAFLAEH FGDPPYWDRI DAR
NT Sequence
ATGAAGGCCG ACCCGATTCC GGCGCCCGAC AAGCCGCTCG ATCTGCTCGA CGTCGGGCTG TTCGTGCGCG CCGCGCTGCT CGCAAACGTG ACGGCCGCCG GGCGCGAATT CGGCGTGTCG GCCGCGGTCG CGAGCGCGCG CATCGCGCAG CTCGAACGCC AGCTCGGCGC GCGGCTGCTG CACCGCACCA CGCGCCGGAT CAGCCTGACG CAGGACGGCG AGATCTTCAT GGCGCGCGCC GAAGCGCTGC TCTCGGCTGC CGACGCCGCG CGCGCATCGG TCGGCCGCGG CCGCACCGAG CCTTACGGGC GGCTGAAGGT GTCGATGTCG TCGTCGTTCG GCCGCCAGCA TGTGACGCCG GTAATTCCGG CGTTCCTGCG CCGCTATCCG TCGGTATCGC TCGACCTGCG GCTGTCCGAC GAAATCGTCG ATCTGGTCGA CGAAGGCTAC GACGTCGCGA TCCGTCTCGG CGCACTGAAG GATTCGACGC TCGTCGCGCG CAAGCTCGCG ACCAACCGCC GCGTGCTGTG CTGCTCGCCC GCCTATCTCG CCGAGCGCGG CACGCCGCGC CATCCGGCCG ATCTCGCGCA GCACGAGTGC GTGATCCTCG GCGACCAGCG CGACTGGTCG TTCGCGACGC CGCAGGGGCC GTTCACGGTG CGCGTCGGCG GCCGGCTCGT CGCGAGCAAC GGCGAGGCGA TCCGCGAAGC GCTGGTCGAC GGCTTCGGGA TCGCGATCAA GTCGACCTGG GACGTCGGCC CGCTGCTCGC GAGCGGCGCG CTCGTCACCG TGCTCGACGA CTATCCGTTC GCCGAGGACA TCGCCGTCTG GGCCGTCTAT CCGAGCCGTG CGCACGTGCC GCTGAAGACG CTCGCGTTCA TCGCGTTCCT CGCCGAGCAT TTCGGCGATC CGCCGTACTG GGACCGCATC GACGCGCGC
Details for BumuA.12800.i.A1.GE34671
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 103
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKADPIPAP DKPLDLLDVG LFVRAALLAN VTAAGREFGV SAAVASARIA QLERQLGARL LHRTTRRISL TQDGEIFMAR AEALLSAADA ARASVGRGRT EPYGRLKVSM SSSFGRQHVT PVIPAFLRRY PSVSLDLRLS DEIVDLVDEG YDVAIRLGAL KDSTLVARKL ATNRRVLCCS PAYLAERGTP RHPADLAQHE CVILGDQRDW SFATPXGPFT VRVGGRLVAS NGXAIREALV DGFGIAIKST WDVGPLLASG ALVTVLDDYP FAXDIXVWXV YPSRAHVPLK XLAXIVXXXH FXXXXTXTXR XXIXXXXXAX SXXXXX
Validated NT Sequence
tttgtttanc tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaaggc cgacccgatt ccggcgcccg acaagccgct cgatctgctc gacgtcgggc tgttcgtgcg cgccgcgctg ctcgcaaacg tgacggccgc cgggcgcgaa ttcggcgtgt cggccgcggt cgcgagcgcg cgcatcgcgc agctcgaacg ccagctcggc gcgcggctgc tgcaccgcac cacgcgccgg atcagcctga cgcaggacgg cgagatcttc atggcgcgcg ccgaagcgct gctctcggct gccgacgccg cgcgcgcatc ggtcggccgc ggccgcaccg agccttacgg gcggctgaag gtgtcgatgt cgtcgtcgtt cggccgccag catgtgacgc cggtaattcc ggcgttcctg cgccgctatc cgtcggtatc gctcgacctg cggctgtccg acgaaatcgt cgatctggtc gacgaaggct acgacgtcgc gatccgtctc ggcgcactga aggattcgac gctcgtcgcg cgcaagctcg cgaccaaccg ccgcgtgctg tgctgctcgc ccgcctatct cgccgagcgc ggcacgccgc gccatccggc cgatctcgcg cagcacgagt gcgtgatcct cggcgaccag cgcgactggt cgttcgcgac gccgcanggg ccgttcacgg tgcgcgtcgg cggccggctc gtcgcgagca acggcgangc gatccgcgaa gcgctggtcg acggcttcgg gatcgcgatc aagtcgacct gggacgtcgg cccgctgctc gcgagcggcg cgctcgtcac cgtgctcgac gactatccgt tcgccnagga catcnnngtc tgggncgtct atccnagccg ngcncacgtg ccgctgaaga ngctcgcgnn catcgtncnt cnnnancatt tcgncgancn nnntactngg acnnntcgac ncncnatann ngnnnnanan tntgcnncna gctnnncnnn nnncgnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKADPIPAP DKPLDLLDVG LFVRAALLAN VTAAGREFGV SAAVASARIA QLERQLGARL LHRTTRRISL TQDGEIFMAR AEALLSAADA ARASVGRGRT EPYGRLKVSM SSSFGRQHVT PVIPAFLRRY PSVSLDLRLS DEIVDLVDEG YDVAIRLGAL KDSTLVARKL ATNRRVLCCS PAYLAERGTP RHPADLAQHE CVILGDQRDW SFATPQGPFT VRVGGRLVAS NGEAIREALV DGFGIAIKST WDVGPLLASG ALVTVLDDYP FAEDIAVWAV YPSRAHVPLK TLAFIAFLAE HFGDPPYWDR IDAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaggccgac ccgattccgg cgcccgacaa gccgctcgat ctgctcgacg tcgggctgtt cgtgcgcgcc gcgctgctcg caaacgtgac ggccgccggg cgcgaattcg gcgtgtcggc cgcggtcgcg agcgcgcgca tcgcgcagct cgaacgccag ctcggcgcgc ggctgctgca ccgcaccacg cgccggatca gcctgacgca ggacggcgag atcttcatgg cgcgcgccga agcgctgctc tcggctgccg acgccgcgcg cgcatcggtc ggccgcggcc gcaccgagcc ttacgggcgg ctgaaggtgt cgatgtcgtc gtcgttcggc cgccagcatg tgacgccggt aattccggcg ttcctgcgcc gctatccgtc ggtatcgctc gacctgcggc tgtccgacga aatcgtcgat ctggtcgacg aaggctacga cgtcgcgatc cgtctcggcg cactgaagga ttcgacgctc gtcgcgcgca agctcgcgac caaccgccgc gtgctgtgct gctcgcccgc ctatctcgcc gagcgcggca cgccgcgcca tccggccgat ctcgcgcagc acgagtgcgt gatcctcggc gaccagcgcg actggtcgtt cgcgacgccg caggggccgt tcacggtgcg cgtcggcggc cggctcgtcg cgagcaacgg cgaggcgatc cgcgaagcgc tggtcgacgg cttcgggatc gcgatcaagt cgacctggga cgtcggcccg ctgctcgcga gcggcgcgct cgtcaccgtg ctcgacgact atccgttcgc cgaggacatc gccgtctggg ccgtctatcc gagccgtgcg cacgtgccgc tgaagacgct cgcgttcatc gcgttcctcg ccgagcattt cggcgatccg ccgtactggg accgcatcga cgcgcgctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa