BumuA.12800.g

LysR family transcriptional regulator

CENTER ID: BumuA.12800.g
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.12800.g.A1.GE31934 Full length( BumuA.12800.g ) 1 294
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.854
RefSeq: YP_001578899.1
UniProt: A0A0H3KH76

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MENLGGFFVF VQVAETRSFV AAGRALGISA SAVGKRIARL EARLNVRLFH RSTRSVTLTA EGARFLERCR RVIAEIDAAE RELTQSVEAP RGRLRVSLPT IGTLMLPVLA GFMTAYPEIE LDLDFSDRLV DVVDEGFDAV VRTGRPADSR LSSRLLGHFR QHLVASPDYL ERHGTPRTPA DLVRHRCLHY RFPATGKLEI WPLRASRSHT PPDVPVSMVS NNAETRLCFA LRGLGIACLP EFFVREPIDA GTLRTVLDDY VASRTPVYVL WPSGHHPTPK LRAFVDYLVE HLQL
NT Sequence
ATGGAGAATC TGGGCGGCTT CTTCGTCTTC GTTCAAGTCG CGGAAACGCG CAGCTTCGTC GCGGCCGGCC GCGCGCTCGG CATCTCCGCG TCGGCGGTCG GCAAGCGCAT CGCGCGGCTC GAGGCGCGGC TCAACGTGCG GCTCTTTCAT CGCAGCACGC GCAGCGTCAC GCTGACGGCC GAGGGCGCGC GCTTTCTCGA ACGATGCCGG CGCGTGATCG CCGAAATCGA TGCGGCCGAG CGCGAGCTCA CGCAAAGCGT CGAAGCGCCG CGCGGGCGGC TGCGCGTGAG CCTGCCGACG ATCGGCACGC TGATGCTGCC GGTGCTCGCC GGCTTCATGA CGGCGTATCC GGAGATCGAA CTGGATCTCG ATTTCAGCGA CCGGCTCGTC GATGTCGTCG ACGAAGGCTT CGATGCGGTC GTGCGCACCG GTCGCCCGGC CGATTCGCGG CTCTCGTCGC GGCTGCTCGG CCATTTCCGC CAGCATCTGG TCGCGTCGCC CGATTACCTC GAACGGCACG GCACGCCGCG CACGCCGGCC GATCTCGTGC GCCATCGCTG CCTGCACTAC CGGTTTCCGG CCACCGGCAA GCTCGAGATC TGGCCGCTGC GTGCATCGCG TTCGCACACG CCGCCGGACG TGCCCGTGTC GATGGTCAGC AACAACGCGG AAACGCGCCT CTGCTTCGCG TTGCGCGGGC TCGGCATCGC GTGCCTGCCC GAGTTCTTCG TGCGCGAGCC GATCGATGCC GGCACGCTGC GCACGGTGCT CGACGATTAC GTCGCGAGCC GCACGCCGGT GTACGTGCTG TGGCCGTCAG GCCACCATCC GACGCCGAAG CTGCGCGCGT TCGTCGACTA CCTCGTCGAG CATCTGCAGC TG
Details for BumuA.12800.g.A1.GE31934
HARVESTED ON: 4/5/2011
SEQUENCED ON: 4/11/2011
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMENLGGF FVFVQVAETR SFVAAGRALG ISASAVGKRI ARLEARLNVR LFHRSTRSVT LTAEGARFLE RCRRVIAEID AAERELTQSV EAPRGRLRVS LPTIGTLMLP VLAGFMTAYP EIELDLDFSD RLVDVVDEGF DAVVRTGRPA DSRLSSRLLG HFRQHLVASP DYLERHGTPR TPADLVRHRC LHYRFPATGK LEIWPLRASR SHTPPDVPVS MVSNNAETRL CFALRGLGIA CLPEFFVREP IDAGTLRTVL DDYVASRTPV YVLWPSGHHP TPKLRAFVDY LVEH
Validated NT Sequence
cagatgctcg acgaggtagt cgacgaacgc gcgcagcttc ggcgtcggat ggtggcctga cggccacagc acgtacaccg gcgtgcggct cgcgacgtaa tcgtcgagca ccgtgcgcag cgtgccggca tcgatcggct cgcgcacgaa gaactcgggc aggcacgcga tgccgagccc gcgcaacgcg aagcagaggc gcgtttccgc gttgttgctg accatcgaca cgggcacgtc cggcggcgtg tgcgaacgcg atgcacgcag cggccagatc tcgagcttgc cggtggccgg aaaccggtag tgcaggcagc gatggcgcac gagatcggcc ggcgtgcgcg gcgtgccgtg ccgttcgagg taatcgggcg acgcgaccag atgctggcgg aaatggccga gcagccgcga cgagagccgc gaatcggccg ggcgaccggt gcgcacgacc gcatcgaagc cttcgtcgac gacatcgacg agccggtcgc tgaaatcgag atccagttcg atctccggat acgccgtcat gaagccggcg agcaccggca gcatcagcgt gccgatcgtc ggcaggctca cgcgcagccg cccgcgcggc gcttcgacgc tttgcgtgag ctcgcgctcg gccgcatcga tttcggcgat cacgcgccgg catcgttcga gaaagcgcgc gccctcggcc gtcagcgtga cgctgcgcgt gctgcgatga aagagccgca cgttgagccg cgcctcgagc cgcgcgatgc gcttgccgac cgccgacgcg gagatgccga gcgcgcggcc ggccgcgacg aagctgcgcg tttccgcgac ttgaacgaag acgaagaagc cgcccagatt ctccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMENLGGFFV FVQVAETRSF VAAGRALGIS ASAVGKRIAR LEARLNVRLF HRSTRSVTLT AEGARFLERC RRVIAEIDAA ERELTQSVEA PRGRLRVSLP TIGTLMLPVL AGFMTAYPEI ELDLDFSDRL VDVVDEGFDA VVRTGRPADS RLSSRLLGHF RQHLVASPDY LERHGTPRTP ADLVRHRCLH YRFPATGKLE IWPLRASRSH TPPDVPVSMV SNNAETRLCF ALRGLGIACL PEFFVREPID AGTLRTVLDD YVASRTPVYV LWPSGHHPTP KLRAFVDYLV EHLQL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagaatctg ggcggcttct tcgtcttcgt tcaagtcgcg gaaacgcgca gcttcgtcgc ggccggccgc gcgctcggca tctccgcgtc ggcggtcggc aagcgcatcg cgcggctcga ggcgcggctc aacgtgcggc tctttcatcg cagcacgcgc agcgtcacgc tgacggccga gggcgcgcgc tttctcgaac gatgccggcg cgtgatcgcc gaaatcgatg cggccgagcg cgagctcacg caaagcgtcg aagcgccgcg cgggcggctg cgcgtgagcc tgccgacgat cggcacgctg atgctgccgg tgctcgccgg cttcatgacg gcgtatccgg agatcgaact ggatctcgat ttcagcgacc ggctcgtcga tgtcgtcgac gaaggcttcg atgcggtcgt gcgcaccggt cgcccggccg attcgcggct ctcgtcgcgg ctgctcggcc atttccgcca gcatctggtc gcgtcgcccg attacctcga acggcacggc acgccgcgca cgccggccga tctcgtgcgc catcgctgcc tgcactaccg gtttccggcc accggcaagc tcgagatctg gccgctgcgt gcatcgcgtt cgcacacgcc gccggacgtg cccgtgtcga tggtcagcaa caacgcggaa acgcgcctct gcttcgcgtt gcgcgggctc ggcatcgcgt gcctgcccga gttcttcgtg cgcgagccga tcgatgccgg cacgctgcgc acggtgctcg acgattacgt cgcgagccgc acgccggtgt acgtgctgtg gccgtcaggc caccatccga cgccgaagct gcgcgcgttc gtcgactacc tcgtcgagca tctgcagctg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa