SSGCID: Target Page

BumuA.12800.f

LysR family transcriptional regulator

CENTER ID:	BumuA.12800.f
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.12800.f.A1.GE34675	Full length( BumuA.12800.f )		1	322

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.789
RefSeq:	YP_001578833.1
UniProt:	A0A0H3KHP9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRIQAMEVF TRVVDANSFT RAAETLAMPR ASVTTIIQNL EALLGVRLMH RTTRRLSLTP EGAAYYEHCV KILSEIAEAD ASFQKGKPTG VLRVHMPSSL GRRLVIPSLS LFRQRYPDIT LDLGLSDRPV DPVEEGIDCM IRVGPLEDSS MVARRIGMLR RVTCAAPDYL ARHGEPREIA DLAGHYAVNF RATQGGRAVP WVFLIDGKPV EVRMNASVTV NDSDAYVNCG LEGFGMIQPT LFMVVSNLLD GSLVEVLPDC NPKPKPISIM YPHNRHLSAK VRVFADWIAE VFESTPALEG GENWRRNVRA KVEGEAAGSV VA

NT Sequence

ATGGACCGGA TCCAGGCAAT GGAAGTTTTC ACGCGCGTGG TCGATGCGAA TAGCTTCACG CGCGCCGCGG AGACGCTCGC GATGCCGCGC GCGTCGGTCA CGACGATCAT CCAGAATCTC GAGGCGTTGC TCGGCGTGCG CCTGATGCAC CGGACGACGC GGCGGCTGTC GCTGACGCCG GAAGGCGCCG CGTACTACGA GCACTGCGTG AAGATTCTGT CGGAAATCGC GGAAGCGGAC GCGAGTTTCC AGAAGGGCAA GCCGACCGGC GTGCTGCGCG TTCACATGCC GAGTTCGCTC GGACGGCGGC TCGTGATTCC GTCGCTGTCG CTGTTCCGGC AGCGCTATCC GGACATCACG CTCGATCTGG GACTGTCCGA TCGGCCGGTC GATCCGGTCG AGGAGGGGAT CGACTGCATG ATTCGCGTGG GGCCGCTCGA GGATTCGTCG ATGGTCGCGC GCCGGATCGG GATGCTCAGA CGCGTGACGT GTGCGGCACC CGACTATCTG GCCCGTCATG GCGAGCCGCG CGAGATCGCC GATCTCGCCG GGCACTACGC CGTGAACTTC CGTGCGACGC AGGGCGGGCG CGCGGTGCCA TGGGTGTTCC TGATCGACGG CAAGCCGGTC GAGGTGCGGA TGAATGCGAG CGTCACGGTC AACGATTCGG ATGCGTACGT GAATTGCGGG CTCGAAGGGT TCGGGATGAT CCAGCCGACG CTGTTCATGG TGGTGTCGAA TCTGCTCGAC GGGTCGCTGG TCGAGGTGCT GCCGGACTGC AATCCGAAGC CGAAGCCGAT TTCGATCATG TATCCGCACA ACCGGCATCT GTCGGCGAAG GTGCGCGTGT TCGCGGACTG GATCGCGGAG GTGTTCGAGT CGACGCCGGC GCTTGAAGGC GGCGAGAACT GGCGGCGGAA TGTGCGGGCG AAGGTTGAGG GTGAGGCGGC GGGGTCGGTG GTGGCT

Details for BumuA.12800.f.A1.GE34675

HARVESTED ON:	1/31/2012
SEQUENCED ON:	3/16/2012
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

aagccaccac cgaccccgcc gcctcaccct caaccttcgc ccgcacattc cgccgccagt tctcgccgcc ttcaagcgcc ggcgtcgact cgaacacctc cgcgatccag tccgcgaaca cgcgcacctt cgccgacaga tgccggttgt gcggatacat gatcgaaatc ggcttcggct tcggattgca gtccggcagc acctcgacca gcgacccgtc gagcagattc gacaccacca tgaacagcgt cggctggatc atcccgaacc cttcgagccc gcaattcacg tacgcatccg aatcgttgac cgtgacgctc gcattcatcc gcacctcgac cggcttgccg tcgatcagga acacccatgg caccgcgcgc ccgccctgcg tcgcacggaa gttcacggcg tagtgcccgg cgagatcggc gatctcgcgc ggctcgccat gacgggccag atagtcgggt gccgcacacg tcacgcgtct gagcatcccg atccggcgcg cgaccatcga cgaatcctcg agcggcccca cgcgaatcat gcagtcgatc ccctcctcga ccggatcgac cggccgatcg gacagtccca gatcgagcgt gatgtccgga tagcgctgcc ggaacagcga cagcgacgga atcacgagcc gccgtccgag cgaactcggc atgtgaacgc gcagcacgcc ggtcggcttg cccttctgga aactcgcgtc cgcttccgcg atttccgaca gaatcttcac gcagtgctcg tagtacgcgg cgccttccgg cgtcagcgac agccgccgcg tcgtccggtg catcaggcgc acgccgagca acgcctcgag attctggatg atcgtcgtga ccgacgcgcg cggcatcgcg agcgtctccg cggcgcgcgt gaagctattc gcatcgacca cgcgcgtgaa aacttccatt gcctggatcc ggtccatcga accaggaccc tgggtctgag cttccagg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDRIQAMEV FTRVVDANSF TRAAETLAMP RASVTTIIQN LEALLGVRLM HRTTRRLSLT PEGAAYYEHC VKILSEIAEA DASFQKGKPT GVLRVHMPSS LGRRLVIPSL SLFRQRYPDI TLDLGLSDRP VDPVEEGIDC MIRVGPLEDS SMVARRIGML RRVTCAAPDY LARHGEPREI ADLAGHYAVN FRATQGGRAV PWVFLIDGKP VEVRMNASVT VNDSDAYVNC GLEGFGMIQP TLFMVVSNLL DGSLVEVLPD CNPKPKPISI MYPHNRHLSA KVRVFADWIA EVFESTPALE GGENWRRNVR AKVEGEAAGS VVA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccggatc caggcaatgg aagttttcac gcgcgtggtc gatgcgaata gcttcacgcg cgccgcggag acgctcgcga tgccgcgcgc gtcggtcacg acgatcatcc agaatctcga ggcgttgctc ggcgtgcgcc tgatgcaccg gacgacgcgg cggctgtcgc tgacgccgga aggcgccgcg tactacgagc actgcgtgaa gattctgtcg gaaatcgcgg aagcggacgc gagtttccag aagggcaagc cgaccggcgt gctgcgcgtt cacatgccga gttcgctcgg acggcggctc gtgattccgt cgctgtcgct gttccggcag cgctatccgg acatcacgct cgatctggga ctgtccgatc ggccggtcga tccggtcgag gaggggatcg actgcatgat tcgcgtgggg ccgctcgagg attcgtcgat ggtcgcgcgc cggatcggga tgctcagacg cgtgacgtgt gcggcacccg actatctggc ccgtcatggc gagccgcgcg agatcgccga tctcgccggg cactacgccg tgaacttccg tgcgacgcag ggcgggcgcg cggtgccatg ggtgttcctg atcgacggca agccggtcga ggtgcggatg aatgcgagcg tcacggtcaa cgattcggat gcgtacgtga attgcgggct cgaagggttc gggatgatcc agccgacgct gttcatggtg gtgtcgaatc tgctcgacgg gtcgctggtc gaggtgctgc cggactgcaa tccgaagccg aagccgattt cgatcatgta tccgcacaac cggcatctgt cggcgaaggt gcgcgtgttc gcggactgga tcgcggaggt gttcgagtcg acgccggcgc ttgaaggcgg cgagaactgg cggcggaatg tgcgggcgaa ggttgagggt gaggcggcgg ggtcggtggt ggcttaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa