SSGCID: Target Page

BumuA.12800.e

LysR family transcriptional regulator

CENTER ID:	BumuA.12800.e
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.12800.e.A1.GE31932	Full length( BumuA.12800.e )		1	327

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.2787
RefSeq:	YP_001580738.1
UniProt:	A0A0H3KL25

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNQIQTMRVF VCVAEHQSFR RAAHHLGVSN ALVTRSIAML ESHLNTRLIH RTTRNLSLTE AGVRYLDGCR ALLEEFDHLE ASVAQATHEP GGTLRVVASG LLSPVALTPL LGRFRHRYPE LRVQMTIAEA PLDVLDAGYD VGIVTGDRFD GNPALIAQAL APNPFVACAS PAYLERRGEP RTPDDLPRHD WVALAPHQHP GAWRLVGPDA RVHTVTVRPA CTVNQLALVH AATVAGSGIA MLPEHCVADA IDSGTLVRLL PGYRIDDPHT QLSLVYPNRQ FVPARTRSFV EHAIEHFSTL AQPERAGYGF LRAAHASAHA DGVPGLQ

NT Sequence

ATGAACCAGA TTCAGACCAT GCGTGTCTTC GTCTGCGTCG CCGAGCATCA GAGCTTCCGG CGTGCGGCGC ATCATCTCGG CGTGTCCAAT GCACTCGTCA CGCGTTCGAT CGCGATGCTC GAGAGCCATC TCAATACGCG GCTGATCCAC CGCACCACGC GCAATCTGTC GCTGACCGAA GCCGGCGTGC GCTATCTCGA CGGCTGCCGC GCGCTGCTCG AGGAATTCGA TCATCTCGAG GCGTCGGTCG CGCAGGCCAC GCACGAGCCG GGCGGCACGC TGCGCGTGGT CGCGTCGGGG CTGCTGTCGC CCGTCGCGCT GACGCCGCTG CTCGGCCGGT TCCGGCATCG CTATCCGGAA TTGCGCGTGC AAATGACGAT CGCCGAGGCG CCGCTCGACG TGCTCGATGC CGGCTACGAC GTCGGCATCG TCACCGGCGA CCGCTTCGAC GGCAACCCGG CGCTGATCGC GCAGGCGCTC GCGCCGAATC CGTTCGTCGC ATGCGCATCG CCCGCGTATC TCGAACGGCG CGGCGAGCCG CGCACGCCCG ACGATCTGCC GCGCCACGAT TGGGTCGCGC TCGCGCCGCA TCAGCATCCG GGCGCATGGC GGCTGGTCGG CCCCGACGCG CGCGTGCATA CGGTCACCGT GCGGCCCGCC TGCACGGTCA ATCAGCTCGC GCTCGTCCAT GCGGCGACGG TCGCGGGCTC GGGCATCGCG ATGCTGCCCG AGCACTGCGT CGCCGACGCG ATCGACAGCG GTACGCTCGT GCGGTTGCTG CCCGGCTACC GGATCGACGA TCCGCATACA CAGCTCTCGC TCGTCTATCC GAACCGGCAG TTCGTGCCGG CACGCACGCG CAGCTTCGTC GAACATGCGA TCGAGCACTT CAGCACGCTC GCGCAGCCCG AGCGGGCGGG CTACGGTTTC CTGCGCGCGG CGCATGCGTC CGCGCATGCC GACGGCGTGC CGGGCCTGCA G

Details for BumuA.12800.e.A1.GE31932

HARVESTED ON:	4/5/2011
SEQUENCED ON:	4/11/2011
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	15
PERCENT COVERAGE:	95

Validated AA Sequence

MRGRMRRAQE TVARPLGLRE RAEVLDRMFD EAARACRHEL PVRIDERELC MRIVDXVAGQ XPHERTAVDR XGDAVLGQHR DARARXXRRM DERELIDRAG GPHGDRMHAR VGADQPPCAR MLMRRERDPI VARQIVGRAR LAAPFEIRGR CACDERIRRE RLRDQRRVAV EAVAGDDADV VAGIEHVERR LGDRHLHAQF RIAMPEPAEQ RRQRDGRQQP RRDHAQRAAR LVRGLRDRRL EMIEFLEQRA AAVEIAHAGF GQRQIARGAV DQPRIEMALE HRDRTRDECI GHAEMMRRTP EALMLGDADE DTHGLNLVHR TRTLGLSFQG T

Validated NT Sequence

ttattactgc aggcccggca cgccgtcggc atgcgcggac gcatgcgccg cgcgcaggaa accgtagccc gcccgctcgg gctgcgcgag cgtgctgaag tgctcgatcg catgttcgac gaagctgcgc gtgcgtgccg gcacgaactg ccggttcgga tagacgagcg agagctgtgt atgcggatcg tcgatncggt agccggncag canccgcacg agcgtaccgc tgtcgatcgc ntnggcgacg cagtgctcgg gcagcatcgn gatgcccgag cccgcgannn tcgccgcatg gacgagcgcg agctgattga ccgtgcaggc gggccgcacg gtgaccgtat gcacgcgcgc gtcggggccg accagccgcc atgcgcccgg atgctgatgc ggcgcgagcg cgacccaatc gtggcgcggc agatcgtcgg gcgtgcgcgg ctcgccgcgc cgttcgagat acgcgggcga tgcgcatgcg acgaacggat tcggcgcgag cgcctgcgcg atcagcgccg ggttgccgtc gaagcggtcg ccggtgacga tgccgacgtc gtagccggca tcgagcacgt cgagcggcgc ctcggcgatc gtcatttgca cgcgcaattc cggatagcga tgccggaacc ggccgagcag cggcgtcagc gcgacgggcg acagcagccc cgacgcgacc acgcgcagcg tgccgcccgg ctcgtgcgtg gcctgcgcga ccgacgcctc gagatgatcg aattcctcga gcagcgcgcg gcagccgtcg agatagcgca cgccggcttc ggtcagcgac agattgcgcg tggtgcggtg gatcagccgc gtattgagat ggctctcgag catcgcgatc gaacgcgtga cgagtgcatt ggacacgccg agatgatgcg ccgcacgccg gaagctctga tgctcggcga cgcagacgaa gacacgcatg gtctgaatct ggttcatcga accaggaccc tgggtctgag cttccagggt accca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNQIQTMRV FVCVAEHQSF RRAAHHLGVS NALVTRSIAM LESHLNTRLI HRTTRNLSLT EAGVRYLDGC RALLEEFDHL EASVAQATHE PGGTLRVVAS GLLSPVALTP LLGRFRHRYP ELRVQMTIAE APLDVLDAGY DVGIVTGDRF DGNPALIAQA LAPNPFVACA SPAYLERRGE PRTPDDLPRH DWVALAPHQH PGAWRLVGPD ARVHTVTVRP ACTVNQLALV HAATVAGSGI AMLPEHCVAD AIDSGTLVRL LPGYRIDDPH TQLSLVYPNR QFVPARTRSF VEHAIEHFST LAQPERAGYG FLRAAHASAH ADGVPGLQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaccagatt cagaccatgc gtgtcttcgt ctgcgtcgcc gagcatcaga gcttccggcg tgcggcgcat catctcggcg tgtccaatgc actcgtcacg cgttcgatcg cgatgctcga gagccatctc aatacgcggc tgatccaccg caccacgcgc aatctgtcgc tgaccgaagc cggcgtgcgc tatctcgacg gctgccgcgc gctgctcgag gaattcgatc atctcgaggc gtcggtcgcg caggccacgc acgagccggg cggcacgctg cgcgtggtcg cgtcggggct gctgtcgccc gtcgcgctga cgccgctgct cggccggttc cggcatcgct atccggaatt gcgcgtgcaa atgacgatcg ccgaggcgcc gctcgacgtg ctcgatgccg gctacgacgt cggcatcgtc accggcgacc gcttcgacgg caacccggcg ctgatcgcgc aggcgctcgc gccgaatccg ttcgtcgcat gcgcatcgcc cgcgtatctc gaacggcgcg gcgagccgcg cacgcccgac gatctgccgc gccacgattg ggtcgcgctc gcgccgcatc agcatccggg cgcatggcgg ctggtcggcc ccgacgcgcg cgtgcatacg gtcaccgtgc ggcccgcctg cacggtcaat cagctcgcgc tcgtccatgc ggcgacggtc gcgggctcgg gcatcgcgat gctgcccgag cactgcgtcg ccgacgcgat cgacagcggt acgctcgtgc ggttgctgcc cggctaccgg atcgacgatc cgcatacaca gctctcgctc gtctatccga accggcagtt cgtgccggca cgcacgcgca gcttcgtcga acatgcgatc gagcacttca gcacgctcgc gcagcccgag cgggcgggct acggtttcct gcgcgcggcg catgcgtccg cgcatgccga cggcgtgccg ggcctgcagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa