SSGCID: Target Page

BumuA.12800.d

LysR family transcriptional regulator

CENTER ID:	BumuA.12800.d
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.12800.d.A1.GE31931	Full length( BumuA.12800.d )		1	294

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.244
RefSeq:	YP_001578321.1
UniProt:	A0A0H3KIK5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDNLNGIVAF VRAADALSFV AAGRTLGISA SAVGKTIARL EQSLGVRLFN RTTRRITLTD EGRHFYERCQ RILEDLRDAE ATVTASAQRP RGKLRVSLPV IGYRFLLPVL PEFRQRYPDV ELDLDFNDRM VDVVEGGFDA VIRSGPLSDS SLMSRRLGPF AFVLCATPAY LARAGTPRAP CDLEAHDCVR YCFPTTGKLQ DWALAADDGT PLKLRTAMTC NNMEALRGAV LAGLGIGYMP DFLARDALER GALVTVLDDY RIAPGQFSIV WPSSRQLSPK LRVFVDFLCE RLFK

NT Sequence

ATGGACAACC TGAACGGCAT CGTCGCCTTC GTGCGCGCCG CCGACGCGCT GAGCTTCGTC GCGGCCGGCC GTACGCTCGG CATCTCGGCG TCGGCGGTCG GCAAGACGAT CGCGCGGCTC GAGCAGTCGC TCGGCGTGCG GCTGTTCAAC CGCACCACGC GCCGCATCAC GCTGACCGAC GAAGGGCGGC ATTTCTACGA GCGCTGCCAG CGCATCCTCG AGGATCTTCG CGACGCGGAG GCGACCGTGA CGGCATCCGC GCAGCGCCCG CGCGGCAAGC TGCGCGTGAG CCTGCCGGTG ATCGGCTACC GGTTCCTGCT GCCGGTGCTG CCGGAATTCA GGCAGCGCTA TCCGGACGTC GAGCTCGATC TGGATTTCAA CGACCGGATG GTCGACGTCG TCGAAGGCGG GTTCGATGCG GTGATCCGTA GCGGGCCGCT ATCCGATTCG AGCCTGATGT CGCGGCGGCT CGGCCCGTTC GCGTTCGTAT TGTGCGCGAC GCCCGCGTAT CTCGCGCGGG CCGGCACACC GCGCGCGCCG TGCGATCTCG AAGCGCACGA CTGCGTGCGC TACTGCTTCC CGACCACGGG CAAGCTGCAG GACTGGGCGC TCGCCGCGGA CGACGGCACG CCGCTGAAGC TGCGCACCGC GATGACGTGC AACAACATGG AAGCGCTGCG CGGCGCGGTG CTCGCGGGGC TCGGCATCGG CTACATGCCG GACTTTCTCG CGCGCGATGC GCTCGAGCGC GGCGCACTCG TCACCGTGCT CGACGACTAC CGGATCGCGC CGGGCCAGTT CTCGATCGTC TGGCCGTCGA GCCGGCAGCT GTCGCCGAAG CTGCGCGTGT TCGTCGACTT TCTCTGCGAG CGGCTGTTCA AG

Details for BumuA.12800.d.A1.GE31931

HARVESTED ON:	4/5/2011
SEQUENCED ON:	4/11/2011
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMDNLNGI VAFVRAADAL SFVAAGRTLG ISASAVGKTI ARLEQSLGVR LFNRTTRRIT LTDEGRHFYE RCQRILEDLR DAEATVTASA QRPRGKLRVS LPVIGYRFLL PVLPEFRQRY PDVELDLDFN DRMVDVVEGG FDAVIRSGPL SDSSLMSRRL GPFAFVLCAT PAYLARAGTP RAPCDLEAHD CVRYCFPTTG KLQDWALAAD DGTPLKLRTA MTCNNMEALR GAVLAGLGIG YMPDFLARDA LERGALVTVL DDYRIAPGQF SIVWPSSRQL SPKLRVFVDF LCERLFK

Validated NT Sequence

ttattacttg aacagccgct cgcagagaaa gtcgacgaac acgcgcagct tcggcgacag ctgccggctc gacggccaga cgatcgagaa ctggcccggc gcgatccggt agtcgtcgag cacggtgacg agtgcgccgc gctcgagcgc atcgcgcgcg agaaagtccg gcatgtagcc gatgccgagc cccgcgagca ccgcgccgcg cagcgcttcc atgttgttgc acgtcatcgc ggtgcgcagc ttcagcggcg tgccgtcgtc cgcggcgagc gcccagtcct gcagcttgcc cgtggtcggg aagcagtagc gcacgcagtc gtgcgcttcg agatcgcacg gcgcgcgcgg tgtgccggcc cgcgcgagat acgcgggcgt cgcgcacaat acgaacgcga acgggccgag ccgccgcgac atcaggctcg aatcggatag cggcccgcta cggatcaccg catcgaaccc gccttcgacg acgtcgacca tccggtcgtt gaaatccaga tcgagctcga cgtccggata gcgctgcctg aattccggca gcaccggcag caggaaccgg tagccgatca ccggcaggct cacgcgcagc ttgccgcgcg ggcgctgcgc ggatgccgtc acggtcgcct ccgcgtcgcg aagatcctcg aggatgcgct ggcagcgctc gtagaaatgc cgcccttcgt cggtcagcgt gatgcggcgc gtggtgcggt tgaacagccg cacgccgagc gactgctcga gccgcgcgat cgtcttgccg accgccgacg ccgagatgcc gagcgtacgg ccggccgcga cgaagctcag cgcgtcggcg gcgcgcacga aggcgacgat gccgttcagg ttgtccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDNLNGIVA FVRAADALSF VAAGRTLGIS ASAVGKTIAR LEQSLGVRLF NRTTRRITLT DEGRHFYERC QRILEDLRDA EATVTASAQR PRGKLRVSLP VIGYRFLLPV LPEFRQRYPD VELDLDFNDR MVDVVEGGFD AVIRSGPLSD SSLMSRRLGP FAFVLCATPA YLARAGTPRA PCDLEAHDCV RYCFPTTGKL QDWALAADDG TPLKLRTAMT CNNMEALRGA VLAGLGIGYM PDFLARDALE RGALVTVLDD YRIAPGQFSI VWPSSRQLSP KLRVFVDFLC ERLFK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaacctg aacggcatcg tcgccttcgt gcgcgccgcc gacgcgctga gcttcgtcgc ggccggccgt acgctcggca tctcggcgtc ggcggtcggc aagacgatcg cgcggctcga gcagtcgctc ggcgtgcggc tgttcaaccg caccacgcgc cgcatcacgc tgaccgacga agggcggcat ttctacgagc gctgccagcg catcctcgag gatcttcgcg acgcggaggc gaccgtgacg gcatccgcgc agcgcccgcg cggcaagctg cgcgtgagcc tgccggtgat cggctaccgg ttcctgctgc cggtgctgcc ggaattcagg cagcgctatc cggacgtcga gctcgatctg gatttcaacg accggatggt cgacgtcgtc gaaggcgggt tcgatgcggt gatccgtagc gggccgctat ccgattcgag cctgatgtcg cggcggctcg gcccgttcgc gttcgtattg tgcgcgacgc ccgcgtatct cgcgcgggcc ggcacaccgc gcgcgccgtg cgatctcgaa gcgcacgact gcgtgcgcta ctgcttcccg accacgggca agctgcagga ctgggcgctc gccgcggacg acggcacgcc gctgaagctg cgcaccgcga tgacgtgcaa caacatggaa gcgctgcgcg gcgcggtgct cgcggggctc ggcatcggct acatgccgga ctttctcgcg cgcgatgcgc tcgagcgcgg cgcactcgtc accgtgctcg acgactaccg gatcgcgccg ggccagttct cgatcgtctg gccgtcgagc cggcagctgt cgccgaagct gcgcgtgttc gtcgactttc tctgcgagcg gctgttcaag taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa