SSGCID: Target Page

BumuA.12800.c

Transcriptional regulator, LysR family

CENTER ID:	BumuA.12800.c
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.12800.c.A1.GE31930	Full length( BumuA.12800.c )		1	310

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.2194
RefSeq:	YP_001580164.1
UniProt:	A9ACH3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MELDSTMASF PTEHLKGIVP FVCVADAGGF TAAAERLNLT NSAVSKSVAR LERRLGVVLF ERTTRKLVLT DAGRAFYETC TRILAELAEA EAVLAAQQRE PVGTVRIALP ASFGRLCVVP VLVRFSEQYP DVRPHLLFTD RFVDLAEEGI DLAVRIGGPP GLPPSLGRRF LGRERLIFCA APAYLEKNGI PQTVDDLQEH ACIVYGRPDG AQSLWSYAAA DGQVEQRVVR PRIVAGDAEA QLAFVRAGLG VAQLATWLVR DDLKAGRLVA ILDEAATDGL PLSLIWPVAR QLTPKIHVLV QTLEAHLHIE

NT Sequence

ATGGAATTGG ATTCAACAAT GGCCAGCTTC CCGACCGAAC ATCTGAAGGG CATCGTTCCA TTCGTCTGTG TCGCCGACGC GGGCGGTTTC ACCGCTGCGG CCGAGCGGCT GAACCTCACG AATTCGGCGG TGAGCAAGAG TGTCGCCCGG CTCGAACGGC GGCTCGGCGT CGTGCTGTTC GAGCGGACGA CGCGCAAGCT CGTGCTCACG GACGCCGGCC GTGCGTTCTA TGAAACCTGC ACGCGCATCC TGGCGGAGCT TGCGGAGGCG GAGGCCGTGC TCGCCGCGCA ACAGCGCGAG CCGGTCGGCA CCGTTCGCAT CGCCTTGCCG GCCTCGTTCG GGCGTCTCTG CGTCGTGCCG GTGCTCGTGC GCTTTTCGGA GCAATATCCG GACGTGCGGC CGCACCTGTT GTTCACGGAC CGCTTCGTCG ATCTGGCAGA AGAGGGCATC GATCTCGCCG TGCGGATCGG CGGGCCGCCG GGTCTGCCGC CGTCGCTCGG TCGGCGTTTT CTCGGGCGCG AACGACTGAT CTTCTGCGCG GCGCCGGCCT ATCTCGAGAA AAACGGCATA CCGCAAACGG TCGACGACCT GCAGGAACAT GCCTGCATCG TCTACGGACG CCCCGACGGA GCGCAGTCGC TGTGGTCATA TGCCGCGGCG GACGGGCAGG TCGAGCAACG CGTCGTGCGG CCACGCATCG TCGCAGGCGA CGCCGAGGCA CAACTCGCAT TCGTACGCGC GGGGCTCGGC GTCGCACAGC TGGCGACATG GCTGGTACGG GACGATCTGA AGGCCGGCCG CCTCGTCGCT ATCCTCGACG AAGCCGCAAC GGACGGCTTG CCGCTCAGCC TCATATGGCC GGTCGCGCGG CAGTTGACGC CGAAGATCCA TGTGCTGGTG CAGACGCTCG AAGCTCATCT GCATATCGAA

Details for BumuA.12800.c.A1.GE31930

HARVESTED ON:	4/5/2011
SEQUENCED ON:	4/11/2011
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	24
PERCENT COVERAGE:	62

Validated AA Sequence

MFLQVVDRLR YAVFLEIGRR RAEDQSFAPE KTPTERRRQT RRPADPHGEI DALFCQIDEA VREQQVRPHV RILLRKAHEH RHDAETPERG RQGDANGADR LALLRGEHGL RLRKLRQDAR AGFIERTAGV REHELARRPL EQHDAEPPFE PGDTLAHRRI REVQPLGRSG ETARVGDTDX XXDALQMFGR EAGPLXNPXP SNQDPGS

Validated NT Sequence

ttattcgata tgcagatgag cttcgagcgt ctgcaccagc acatggatct tcggcgtcaa ctgccgcgcg accggccata tgaggctgag cggcaagccg tccgttgcgg cttcgtcgag gatagcgacg aggcggccgg ccttcagatc gtcccgtacc agccatgtcg ccagctgtgc gacgccgagc cccgcgcgta cgaatgcgag ttgtgcctcg gcgtcgcctg cgacgatgcg tggccgcacg acgcgttgct cgacctgccc gtccgccgcg gcatatgacc acagcgactg cgctccgtcg gggcgtccgt agacgatgca ggcatgttcc tgcaggtcgt cgaccgtttg cggtatgccg tttttctcga gataggccgg cgccgcgcag aagatcagtc gttcgcgccc gagaaaacgc cgaccgagcg acggcggcag acccggcggc ccgccgatcc gcacggcgag atcgatgccc tcttctgcca gatcgacgaa gcggtccgtg aacaacaggt gcggccgcac gtccggatat tgctccgaaa agcgcacgag caccggcacg acgcagagac gcccgaacga ggccggcaag gcgatgcgaa cggtgccgac cggctcgcgc tgttgcgcgg cgagcacggc ctccgcctcc gcaagctccg ccaggatgcg cgtgcaggtt tcatagaacg cacggccggc gtccgtgagc acgagcttgc gcgtcgtccg ctcgaacagc acgacgcnga gccgccgttc gagccgggcg acactcttgc tcaccgccga attcgtgagg ttcagccgct cggccgcagc ggtgaaaccg cccgcgtcgg cgacacagac naangganng atgcccttca gatgttcggt cgggaagctg ggccattgnn gaatccaatn ccatcgaacc aggaccctgg gtctga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMELDSTMAS FPTEHLKGIV PFVCVADAGG FTAAAERLNL TNSAVSKSVA RLERRLGVVL FERTTRKLVL TDAGRAFYET CTRILAELAE AEAVLAAQQR EPVGTVRIAL PASFGRLCVV PVLVRFSEQY PDVRPHLLFT DRFVDLAEEG IDLAVRIGGP PGLPPSLGRR FLGRERLIFC AAPAYLEKNG IPQTVDDLQE HACIVYGRPD GAQSLWSYAA ADGQVEQRVV RPRIVAGDAE AQLAFVRAGL GVAQLATWLV RDDLKAGRLV AILDEAATDG LPLSLIWPVA RQLTPKIHVL VQTLEAHLHI E

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaattggat tcaacaatgg ccagcttccc gaccgaacat ctgaagggca tcgttccatt cgtctgtgtc gccgacgcgg gcggtttcac cgctgcggcc gagcggctga acctcacgaa ttcggcggtg agcaagagtg tcgcccggct cgaacggcgg ctcggcgtcg tgctgttcga gcggacgacg cgcaagctcg tgctcacgga cgccggccgt gcgttctatg aaacctgcac gcgcatcctg gcggagcttg cggaggcgga ggccgtgctc gccgcgcaac agcgcgagcc ggtcggcacc gttcgcatcg ccttgccggc ctcgttcggg cgtctctgcg tcgtgccggt gctcgtgcgc ttttcggagc aatatccgga cgtgcggccg cacctgttgt tcacggaccg cttcgtcgat ctggcagaag agggcatcga tctcgccgtg cggatcggcg ggccgccggg tctgccgccg tcgctcggtc ggcgttttct cgggcgcgaa cgactgatct tctgcgcggc gccggcctat ctcgagaaaa acggcatacc gcaaacggtc gacgacctgc aggaacatgc ctgcatcgtc tacggacgcc ccgacggagc gcagtcgctg tggtcatatg ccgcggcgga cgggcaggtc gagcaacgcg tcgtgcggcc acgcatcgtc gcaggcgacg ccgaggcaca actcgcattc gtacgcgcgg ggctcggcgt cgcacagctg gcgacatggc tggtacggga cgatctgaag gccggccgcc tcgtcgctat cctcgacgaa gccgcaacgg acggcttgcc gctcagcctc atatggccgg tcgcgcggca gttgacgccg aagatccatg tgctggtgca gacgctcgaa gctcatctgc atatcgaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a