BumuA.12800.b

LysR family transcriptional regulator

CENTER ID: BumuA.12800.b
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.12800.b.A1.GE31929 Full length( BumuA.12800.b ) 1 349
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.12800.b.A1.PS01087 Full length( BumuA.12800.b ) 1 349

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.3376
RefSeq: YP_001581309.1
UniProt: A0A0H3KB13

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDHLQSMRVF VKVADLGSFA RAASAMDISN AVATRHVADL EGRLGTRLLN RTTRSLSLTE SGQVYLERAR QILDELEDVE QMVVARNHEP VGTLRIVAPV VFGLHNLAPV LQSYTEQFPK VVPDLTLVDR QVDLVEEGFD VGIVVTRQMR SASIVTRRLT TGCMTVCATP SYLEKHGVPT HPEQLAEHPS LSLPTEYWGD ERVFTGPDGE VRVRPTNVIV ANNTAMLRQF ALLGMGVAIL PSYLIGGDIA RGALVRLLPD FKLPQVEINI AYPSRRHLPA KVRTFIDHLV EYFSKSTDAT MGEQWAAQGA TLPPIGVEAR AEQPEPADPN LSPRLPRAGR QRVSAPSPL
NT Sequence
ATGGATCATT TGCAGTCGAT GCGCGTGTTC GTCAAGGTTG CGGATCTCGG CAGTTTCGCG CGGGCCGCGA GTGCAATGGA TATCTCGAAC GCGGTCGCGA CGCGCCATGT CGCCGATCTC GAAGGCCGGC TCGGCACGCG GCTGCTGAAT CGCACGACGC GCAGCCTTTC GCTGACGGAA TCGGGGCAGG TCTATCTGGA GCGTGCGCGC CAGATTCTGG ACGAGCTCGA GGACGTCGAG CAGATGGTCG TCGCGCGCAA TCACGAGCCG GTCGGCACGC TGCGCATCGT CGCGCCCGTC GTGTTCGGCC TGCATAACCT CGCACCGGTG CTGCAGTCGT ACACCGAACA GTTCCCGAAA GTGGTGCCCG ATCTGACGCT CGTCGATCGG CAGGTCGATC TCGTCGAGGA AGGCTTCGAC GTCGGCATCG TCGTCACGCG ACAGATGCGC AGTGCGAGCA TCGTCACGCG GCGTCTGACC ACGGGCTGCA TGACGGTATG CGCGACGCCG AGCTATCTGG AAAAGCACGG CGTGCCGACG CATCCGGAGC AGCTCGCCGA GCATCCGAGC CTGAGTCTGC CGACCGAATA CTGGGGCGAC GAGCGCGTGT TTACGGGGCC CGACGGCGAA GTGCGCGTGC GTCCGACCAA CGTGATCGTC GCGAACAACA CCGCGATGCT GCGGCAGTTC GCGCTGCTCG GAATGGGCGT CGCGATCCTG CCGAGCTACC TGATCGGCGG CGACATCGCG CGCGGTGCGC TCGTGCGGCT GCTGCCCGAT TTCAAGCTGC CGCAGGTCGA GATCAACATC GCCTATCCGA GCCGGCGCCA TCTGCCGGCG AAGGTGCGTA CGTTCATCGA TCACCTCGTC GAGTACTTCA GCAAGTCGAC CGACGCGACG ATGGGCGAGC AGTGGGCCGC GCAGGGCGCG ACGCTCCCGC CGATCGGCGT CGAAGCGCGT GCGGAGCAGC CGGAACCGGC AGACCCGAAC CTGTCGCCGC GGCTGCCGCG CGCAGGCCGT CAGCGCGTCT CGGCGCCGTC GCCGCTC
Details for BumuA.12800.b.A1.GE31929
HARVESTED ON: 4/5/2011
SEQUENCED ON: 4/11/2011
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MDHLQSMRVF VKVADLGSFA RAASAMDISN AVATRHVADL EGRLGTRLLN RTTRSLSLTE SGQVYLERAR QILDELEDVE QMVVARNHEP VGTLRIVAPV VFGLHNLAPV LQSYTEQFPK VVPDLTLVDR QVDLVEEGFD VGIVVTRQMR SASIVTRRLT TGCMTVCATP SYLEKHGVPT HPEQLAEHPS LSLPTEYWGD ERVFTGPDGE VRVRPTNVIV ANNTAMLRQF ALLGMGVAIL PSYLIGGDIA RGALVRLLPD FKLPQVEINI AYPSRRHLPA KVRTFIDHLV EYFSKSTDAT MGEQWAAQGA TLPPIGVEAR AEQPEPADPN LSPRLPRAGR QRVSAPS
Validated NT Sequence
ggcgacggcg ccgagacgcg ctgacggcct gcgcgcggca gccgcggcga caggttcggg tctgccggtt ccggctgctc cgcacgcgct tcgacgccga tcggcgggag cgtcgcgccc tgcgcggccc actgctcgcc catcgtcgcg tcggtcgact tgctgaagta ctcgacgagg tgatcgatga acgtacgcac cttcgccggc agatggcgcc ggctcggata ggcgatgttg atctcgacct gcggcagctt gaaatcgggc agcagccgca cgagcgcacc gcgcgcgatg tcgccgccga tcaggtagct cggcaggatc gcgacgccca ttccgagcag cgcgaactgc cgcagcatcg cggtgttgtt cgcgacgatc acgttggtcg gacgcacgcg cacttcgccg tcgggccccg taaacacgcg ctcgtcgccc cagtattcgg tcggcagact caggctcgga tgctcggcga gctgctccgg atgcgtcggc acgccgtgct tttccagata gctcggcgtc gcgcataccg tcatgcagcc cgtggtcaga cgccgcgtga cgatgctcgc actgcgcatc tgtcgcgtga cgacgatgcc gacgtcgaag ccttcctcga cgagatcgac ctgccgatcg acgagcgtca gatcgggcac cactttcggg aactgttcgg tgtacgactg cagcaccggt gcgaggttat gcaggccgaa cacgacgggc gcgacgatgc gcagcgtgcc gaccggctcg tgattgcgcg cgacgaccat ctgctcgacg tcctcgagct cgtccagaat ctggcgcgca cgctccagat agacctgccc cgattccgtc agcgaaaggc tgcgcgtcgt gcgattcagc agccgcgtgc cgagccggcc ttcgagatcg gcgacatggc gcgtcgcgac cgcgttcgag atatccattg cactcgcggc ccgcgcgaaa ctgccgagat ccgcaacctt gacgaacacg cgcatcgact gcaaatgatc catcgaacca ggaccctggg tctgagcttc cagggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDHLQSMRV FVKVADLGSF ARAASAMDIS NAVATRHVAD LEGRLGTRLL NRTTRSLSLT ESGQVYLERA RQILDELEDV EQMVVARNHE PVGTLRIVAP VVFGLHNLAP VLQSYTEQFP KVVPDLTLVD RQVDLVEEGF DVGIVVTRQM RSASIVTRRL TTGCMTVCAT PSYLEKHGVP THPEQLAEHP SLSLPTEYWG DERVFTGPDG EVRVRPTNVI VANNTAMLRQ FALLGMGVAI LPSYLIGGDI ARGALVRLLP DFKLPQVEIN IAYPSRRHLP AKVRTFIDHL VEYFSKSTDA TMGEQWAAQG ATLPPIGVEA RAEQPEPADP NLSPRLPRAG RQRVSAPSPL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatcatttg cagtcgatgc gcgtgttcgt caaggttgcg gatctcggca gtttcgcgcg ggccgcgagt gcaatggata tctcgaacgc ggtcgcgacg cgccatgtcg ccgatctcga aggccggctc ggcacgcggc tgctgaatcg cacgacgcgc agcctttcgc tgacggaatc ggggcaggtc tatctggagc gtgcgcgcca gattctggac gagctcgagg acgtcgagca gatggtcgtc gcgcgcaatc acgagccggt cggcacgctg cgcatcgtcg cgcccgtcgt gttcggcctg cataacctcg caccggtgct gcagtcgtac accgaacagt tcccgaaagt ggtgcccgat ctgacgctcg tcgatcggca ggtcgatctc gtcgaggaag gcttcgacgt cggcatcgtc gtcacgcgac agatgcgcag tgcgagcatc gtcacgcggc gtctgaccac gggctgcatg acggtatgcg cgacgccgag ctatctggaa aagcacggcg tgccgacgca tccggagcag ctcgccgagc atccgagcct gagtctgccg accgaatact ggggcgacga gcgcgtgttt acggggcccg acggcgaagt gcgcgtgcgt ccgaccaacg tgatcgtcgc gaacaacacc gcgatgctgc ggcagttcgc gctgctcgga atgggcgtcg cgatcctgcc gagctacctg atcggcggcg acatcgcgcg cggtgcgctc gtgcggctgc tgcccgattt caagctgccg caggtcgaga tcaacatcgc ctatccgagc cggcgccatc tgccggcgaa ggtgcgtacg ttcatcgatc acctcgtcga gtacttcagc aagtcgaccg acgcgacgat gggcgagcag tgggccgcgc agggcgcgac gctcccgccg atcggcgtcg aagcgcgtgc ggagcagccg gaaccggcag acccgaacct gtcgccgcgg ctgccgcgcg caggccgtca gcgcgtctcg gcgccgtcgc cgctctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BumuA.12800.b.A1.PS01087
PURIFICATION DATe: 5/12/2011
CONCENTRATION: 23.007mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MDHLQSMRVF VKVADLGSFA RAASAMDISN AVATRHVADL EGRLGTRLLN RTTRSLSLTE SGQVYLERAR QILDELEDVE QMVVARNHEP VGTLRIVAPV VFGLHNLAPV LQSYTEQFPK VVPDLTLVDR QVDLVEEGFD VGIVVTRQMR SASIVTRRLT TGCMTVCATP SYLEKHGVPT HPEQLAEHPS LSLPTEYWGD ERVFTGPDGE VRVRPTNVIV ANNTAMLRQF ALLGMGVAIL PSYLIGGDIA RGALVRLLPD FKLPQVEINI AYPSRRHLPA KVRTFIDHLV EYFSKSTDAT MGEQWAAQGA TLPPIGVEAR AEQPEPADPN LSPRLPRAGR QRVSAPS
Validated NT Sequence
ggcgacggcg ccgagacgcg ctgacggcct gcgcgcggca gccgcggcga caggttcggg tctgccggtt ccggctgctc cgcacgcgct tcgacgccga tcggcgggag cgtcgcgccc tgcgcggccc actgctcgcc catcgtcgcg tcggtcgact tgctgaagta ctcgacgagg tgatcgatga acgtacgcac cttcgccggc agatggcgcc ggctcggata ggcgatgttg atctcgacct gcggcagctt gaaatcgggc agcagccgca cgagcgcacc gcgcgcgatg tcgccgccga tcaggtagct cggcaggatc gcgacgccca ttccgagcag cgcgaactgc cgcagcatcg cggtgttgtt cgcgacgatc acgttggtcg gacgcacgcg cacttcgccg tcgggccccg taaacacgcg ctcgtcgccc cagtattcgg tcggcagact caggctcgga tgctcggcga gctgctccgg atgcgtcggc acgccgtgct tttccagata gctcggcgtc gcgcataccg tcatgcagcc cgtggtcaga cgccgcgtga cgatgctcgc actgcgcatc tgtcgcgtga cgacgatgcc gacgtcgaag ccttcctcga cgagatcgac ctgccgatcg acgagcgtca gatcgggcac cactttcggg aactgttcgg tgtacgactg cagcaccggt gcgaggttat gcaggccgaa cacgacgggc gcgacgatgc gcagcgtgcc gaccggctcg tgattgcgcg cgacgaccat ctgctcgacg tcctcgagct cgtccagaat ctggcgcgca cgctccagat agacctgccc cgattccgtc agcgaaaggc tgcgcgtcgt gcgattcagc agccgcgtgc cgagccggcc ttcgagatcg gcgacatggc gcgtcgcgac cgcgttcgag atatccattg cactcgcggc ccgcgcgaaa ctgccgagat ccgcaacctt gacgaacacg cgcatcgact gcaaatgatc catcgaacca ggaccctggg tctgagcttc cagggta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDHLQSMRV FVKVADLGSF ARAASAMDIS NAVATRHVAD LEGRLGTRLL NRTTRSLSLT ESGQVYLERA RQILDELEDV EQMVVARNHE PVGTLRIVAP VVFGLHNLAP VLQSYTEQFP KVVPDLTLVD RQVDLVEEGF DVGIVVTRQM RSASIVTRRL TTGCMTVCAT PSYLEKHGVP THPEQLAEHP SLSLPTEYWG DERVFTGPDG EVRVRPTNVI VANNTAMLRQ FALLGMGVAI LPSYLIGGDI ARGALVRLLP DFKLPQVEIN IAYPSRRHLP AKVRTFIDHL VEYFSKSTDA TMGEQWAAQG ATLPPIGVEA RAEQPEPADP NLSPRLPRAG RQRVSAPSPL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATCATTTG CAGTCGATGC GCGTGTTCGT CAAGGTTGCG GATCTCGGCA GTTTCGCGCG GGCCGCGAGT GCAATGGATA TCTCGAACGC GGTCGCGACG CGCCATGTCG CCGATCTCGA AGGCCGGCTC GGCACGCGGC TGCTGAATCG CACGACGCGC AGCCTTTCGC TGACGGAATC GGGGCAGGTC TATCTGGAGC GTGCGCGCCA GATTCTGGAC GAGCTCGAGG ACGTCGAGCA GATGGTCGTC GCGCGCAATC ACGAGCCGGT CGGCACGCTG CGCATCGTCG CGCCCGTCGT GTTCGGCCTG CATAACCTCG CACCGGTGCT GCAGTCGTAC ACCGAACAGT TCCCGAAAGT GGTGCCCGAT CTGACGCTCG TCGATCGGCA GGTCGATCTC GTCGAGGAAG GCTTCGACGT CGGCATCGTC GTCACGCGAC AGATGCGCAG TGCGAGCATC GTCACGCGGC GTCTGACCAC GGGCTGCATG ACGGTATGCG CGACGCCGAG CTATCTGGAA AAGCACGGCG TGCCGACGCA TCCGGAGCAG CTCGCCGAGC ATCCGAGCCT GAGTCTGCCG ACCGAATACT GGGGCGACGA GCGCGTGTTT ACGGGGCCCG ACGGCGAAGT GCGCGTGCGT CCGACCAACG TGATCGTCGC GAACAACACC GCGATGCTGC GGCAGTTCGC GCTGCTCGGA ATGGGCGTCG CGATCCTGCC GAGCTACCTG ATCGGCGGCG ACATCGCGCG CGGTGCGCTC GTGCGGCTGC TGCCCGATTT CAAGCTGCCG CAGGTCGAGA TCAACATCGC CTATCCGAGC CGGCGCCATC TGCCGGCGAA GGTGCGTACG TTCATCGATC ACCTCGTCGA GTACTTCAGC AAGTCGACCG ACGCGACGAT GGGCGAGCAG TGGGCCGCGC AGGGCGCGAC GCTCCCGCCG ATCGGCGTCG AAGCGCGTGC GGAGCAGCCG GAACCGGCAG ACCCGAACCT GTCGCCGCGG CTGCCGCGCG CAGGCCGTCA GCGCGTCTCG GCGCCGTCGC CGCTCTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA