BumuA.12800.a

LysR family transcriptional regulator

CENTER ID: BumuA.12800.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.12800.a.A1.GE31928 Full length( BumuA.12800.a ) 1 313
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.12800.a.A1.PS01216 Full length( BumuA.12800.a ) 1 313 unavailable

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.2069
RefSeq: YP_001580049.1
UniProt: A0A0H3KIZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDTLQNMRVF VRVVDAGSFT AAAQQMNSTT AYASRAVSDL EAHLRTRLLN RTTRRIALTE AGERYLQRCE QILAYVDQAE AEAGDAHARP SGKLKVHCFT SLGQHYLVPA VARYRERYPD VHVELTLAQR MPDLLDEGYD VAIVVGRDLP DSGLVSQRLG ESYSVVCAST AYVAAHGAPQ CPADLAQHVC LGMVAPGFHY DEWTLTGPDG DESVPVTAPP FRVNVAEALA VAVREGMGIG GLPLYSAIGG LRSGQIVRVM PEYRSHVMNI YALYASRQYL DAKIRTWVDF LRDALPTTLA ADEAALEQCM RAT
NT Sequence
ATGGATACGT TACAAAACAT GCGGGTATTC GTTCGCGTGG TCGACGCCGG TAGCTTCACC GCCGCCGCCC AGCAGATGAA TTCGACGACC GCCTATGCGT CGCGCGCGGT GTCCGATCTG GAGGCGCATC TGCGCACGCG GCTGCTGAAC CGCACGACGC GCCGGATCGC GCTGACCGAA GCCGGCGAGC GCTATCTGCA GCGCTGCGAG CAGATCCTCG CGTATGTCGA CCAAGCCGAA GCCGAGGCAG GCGACGCGCA TGCGCGCCCG TCCGGCAAGC TGAAGGTCCA CTGCTTCACG AGCCTCGGCC AGCACTACCT GGTGCCGGCC GTCGCGCGCT ACCGCGAGCG CTATCCCGAC GTACACGTCG AGCTGACGCT CGCGCAGCGG ATGCCCGATC TGCTCGACGA GGGGTACGAC GTCGCGATCG TCGTCGGCCG CGATCTGCCC GATTCGGGGC TCGTGTCGCA GCGGCTCGGC GAGAGCTACA GCGTCGTGTG CGCGTCGACC GCCTACGTCG CCGCGCATGG CGCGCCGCAG TGTCCGGCCG ACCTCGCGCA GCACGTGTGC CTCGGGATGG TCGCGCCCGG TTTCCACTAC GACGAATGGA CGCTGACCGG CCCCGACGGC GACGAGTCGG TGCCCGTCAC TGCGCCGCCG TTTCGCGTGA ACGTCGCCGA GGCGCTGGCC GTCGCCGTGC GGGAAGGGAT GGGGATCGGC GGGCTGCCGC TGTATTCGGC GATCGGCGGG CTGCGCAGCG GGCAGATCGT GCGCGTGATG CCCGAATACC GGTCTCACGT GATGAACATC TACGCGCTGT ATGCGTCGCG CCAGTATCTC GACGCAAAAA TCCGCACCTG GGTCGATTTC CTGCGCGACG CATTGCCGAC GACGCTCGCC GCCGACGAAG CGGCCCTCGA ACAGTGCATG CGCGCGACA
Details for BumuA.12800.a.A1.GE31928
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 79
Validated AA Sequence
MGTLEAQTQG PGSMDTLQNM RVFVRVVDAG SFTAAAQQMN STTAYASRAV SDLEAHLRTR LLNRTTRRIA LTEAGERYLQ RCEQILAYVD QAEAEAGDAH ARPSGKLKVH CFTSLGQHYL VPAVARYRER YPDVHVELTL AQRMPDLLDE GYDVAIVVGR DLPDSGLVSQ RLGESYSVVC ASTAYVAAHG APQCPADLAQ HVCLGMVAPG FHYDEWTLTG PDGDESVPVT APPFRVNVAE ALAVAVREGM GIGGLPLYSA IGGLRSG
Validated NT Sequence
gcccgctgcg cagcccgccg atcgccgaat acagcggcag cccgccgatc cccatccctt cccgcacggc gacggccagc gcctcggcga cgttcacgcg aaacggcggc gcagtgacgg gcaccgactc gtcgccgtcg gggccggtca gcgtccattc gtcgtagtgg aaaccgggcg cgaccatccc gaggcacacg tgctgcgcga ggtcggccgg acactgcggc gcgccatgcg cggcgacgta ggcggtcgac gcgcacacga cgctgtagct ctcgccgagc cgctgcgaca cgagccccga atcgggcaga tcgcggccga cgacgatcgc gacgtcgtac ccctcgtcga gcagatcggg catccgctgc gcgagcgtca gctcgacgtg tacgtcggga tagcgctcgc ggtagcgcgc gacggccggc accaggtagt gctggccgag gctcgtgaag cagtggacct tcagcttgcc ggacgggcgc gcatgcgcgt cgcctgcctc ggcttcggct tggtcgacat acgcgaggat ctgctcgcag cgctgcagat agcgctcgcc ggcttcggtc agcgcgatcc ggcgcgtcgt gcggttcagc agccgcgtgc gcagatgcgc ctccagatcg gacaccgcgc gcgacgcata ggcggtcgtc gaattcatct gctgggcggc ggcggtgaag ctaccggcgt cgaccacgcg aacgaatacc cgcatgtttt gtaacgtatc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt ganggtgatn nnnca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDTLQNMRV FVRVVDAGSF TAAAQQMNST TAYASRAVSD LEAHLRTRLL NRTTRRIALT EAGERYLQRC EQILAYVDQA EAEAGDAHAR PSGKLKVHCF TSLGQHYLVP AVARYRERYP DVHVELTLAQ RMPDLLDEGY DVAIVVGRDL PDSGLVSQRL GESYSVVCAS TAYVAAHGAP QCPADLAQHV CLGMVAPGFH YDEWTLTGPD GDESVPVTAP PFRVNVAEAL AVAVREGMGI GGLPLYSAIG GLRSGQIVRV MPEYRSHVMN IYALYASRQY LDAKIRTWVD FLRDALPTTL AADEAALEQC MRAT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatacgtta caaaacatgc gggtattcgt tcgcgtggtc gacgccggta gcttcaccgc cgccgcccag cagatgaatt cgacgaccgc ctatgcgtcg cgcgcggtgt ccgatctgga ggcgcatctg cgcacgcggc tgctgaaccg cacgacgcgc cggatcgcgc tgaccgaagc cggcgagcgc tatctgcagc gctgcgagca gatcctcgcg tatgtcgacc aagccgaagc cgaggcaggc gacgcgcatg cgcgcccgtc cggcaagctg aaggtccact gcttcacgag cctcggccag cactacctgg tgccggccgt cgcgcgctac cgcgagcgct atcccgacgt acacgtcgag ctgacgctcg cgcagcggat gcccgatctg ctcgacgagg ggtacgacgt cgcgatcgtc gtcggccgcg atctgcccga ttcggggctc gtgtcgcagc ggctcggcga gagctacagc gtcgtgtgcg cgtcgaccgc ctacgtcgcc gcgcatggcg cgccgcagtg tccggccgac ctcgcgcagc acgtgtgcct cgggatggtc gcgcccggtt tccactacga cgaatggacg ctgaccggcc ccgacggcga cgagtcggtg cccgtcactg cgccgccgtt tcgcgtgaac gtcgccgagg cgctggccgt cgccgtgcgg gaagggatgg ggatcggcgg gctgccgctg tattcggcga tcggcgggct gcgcagcggg cagatcgtgc gcgtgatgcc cgaataccgg tctcacgtga tgaacatcta cgcgctgtat gcgtcgcgcc agtatctcga cgcaaaaatc cgcacctggg tcgatttcct gcgcgacgca ttgccgacga cgctcgccgc cgacgaagcg gccctcgaac agtgcatgcg cgcgacataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BumuA.12800.a.A1.PS01216
PURIFICATION DATe: 9/21/2011
CONCENTRATION: 1.95mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 79
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMDTLQNM RVFVRVVDAG SFTAAAQQMN STTAYASRAV SDLEAHLRTR LLNRTTRRIA LTEAGERYLQ RCEQILAYVD QAEAEAGDAH ARPSGKLKVH CFTSLGQHYL VPAVARYRER YPDVHVELTL AQRMPDLLDE GYDVAIVVGR DLPDSGLVSQ RLGESYSVVC ASTAYVAAHG APQCPADLAQ HVCLGMVAPG FHYDEWTLTG PDGDESVPVT APPFRVNVAE ALAVAVREGM GIGGLPLYSA IGGLRSG
Validated NT Sequence
gcccgctgcg cagcccgccg atcgccgaat acagcggcag cccgccgatc cccatccctt cccgcacggc gacggccagc gcctcggcga cgttcacgcg aaacggcggc gcagtgacgg gcaccgactc gtcgccgtcg gggccggtca gcgtccattc gtcgtagtgg aaaccgggcg cgaccatccc gaggcacacg tgctgcgcga ggtcggccgg acactgcggc gcgccatgcg cggcgacgta ggcggtcgac gcgcacacga cgctgtagct ctcgccgagc cgctgcgaca cgagccccga atcgggcaga tcgcggccga cgacgatcgc gacgtcgtac ccctcgtcga gcagatcggg catccgctgc gcgagcgtca gctcgacgtg tacgtcggga tagcgctcgc ggtagcgcgc gacggccggc accaggtagt gctggccgag gctcgtgaag cagtggacct tcagcttgcc ggacgggcgc gcatgcgcgt cgcctgcctc ggcttcggct tggtcgacat acgcgaggat ctgctcgcag cgctgcagat agcgctcgcc ggcttcggtc agcgcgatcc ggcgcgtcgt gcggttcagc agccgcgtgc gcagatgcgc ctccagatcg gacaccgcgc gcgacgcata ggcggtcgtc gaattcatct gctgggcggc ggcggtgaag ctaccggcgt cgaccacgcg aacgaatacc cgcatgtttt gtaacgtatc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt ganggtgatn nnnca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDTLQNMRV FVRVVDAGSF TAAAQQMNST TAYASRAVSD LEAHLRTRLL NRTTRRIALT EAGERYLQRC EQILAYVDQA EAEAGDAHAR PSGKLKVHCF TSLGQHYLVP AVARYRERYP DVHVELTLAQ RMPDLLDEGY DVAIVVGRDL PDSGLVSQRL GESYSVVCAS TAYVAAHGAP QCPADLAQHV CLGMVAPGFH YDEWTLTGPD GDESVPVTAP PFRVNVAEAL AVAVREGMGI GGLPLYSAIG GLRSGQIVRV MPEYRSHVMN IYALYASRQY LDAKIRTWVD FLRDALPTTL AADEAALEQC MRAT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATACGTTA CAAAACATGC GGGTATTCGT TCGCGTGGTC GACGCCGGTA GCTTCACCGC CGCCGCCCAG CAGATGAATT CGACGACCGC CTATGCGTCG CGCGCGGTGT CCGATCTGGA GGCGCATCTG CGCACGCGGC TGCTGAACCG CACGACGCGC CGGATCGCGC TGACCGAAGC CGGCGAGCGC TATCTGCAGC GCTGCGAGCA GATCCTCGCG TATGTCGACC AAGCCGAAGC CGAGGCAGGC GACGCGCATG CGCGCCCGTC CGGCAAGCTG AAGGTCCACT GCTTCACGAG CCTCGGCCAG CACTACCTGG TGCCGGCCGT CGCGCGCTAC CGCGAGCGCT ATCCCGACGT ACACGTCGAG CTGACGCTCG CGCAGCGGAT GCCCGATCTG CTCGACGAGG GGTACGACGT CGCGATCGTC GTCGGCCGCG ATCTGCCCGA TTCGGGGCTC GTGTCGCAGC GGCTCGGCGA GAGCTACAGC GTCGTGTGCG CGTCGACCGC CTACGTCGCC GCGCATGGCG CGCCGCAGTG TCCGGCCGAC CTCGCGCAGC ACGTGTGCCT CGGGATGGTC GCGCCCGGTT TCCACTACGA CGAATGGACG CTGACCGGCC CCGACGGCGA CGAGTCGGTG CCCGTCACTG CGCCGCCGTT TCGCGTGAAC GTCGCCGAGG CGCTGGCCGT CGCCGTGCGG GAAGGGATGG GGATCGGCGG GCTGCCGCTG TATTCGGCGA TCGGCGGGCT GCGCAGCGGG CAGATCGTGC GCGTGATGCC CGAATACCGG TCTCACGTGA TGAACATCTA CGCGCTGTAT GCGTCGCGCC AGTATCTCGA CGCAAAAATC CGCACCTGGG TCGATTTCCT GCGCGACGCA TTGCCGACGA CGCTCGCCGC CGACGAAGCG GCCCTCGAAC AGTGCATGCG CGCGACATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA