SSGCID: Target Page

BumuA.12797.a

N-acylmannosamine kinase

CENTER ID:	BumuA.12797.a
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.12797.a.B1.GE39715	Full length( BumuA.12797.a )		1	416

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.1922
RefSeq:	YP_001579895.1
UniProt:	A0A0H3KJB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRSPHIGQGS NSANVRRYNE RLLLKTLRRA GSASKADLAR LANMTGTAVG SIIASLADAK LIEFAGRRTE GQRGQPASLI RLDPRGAFGI GVHLDRMRIE TALVNFAGDV LGRRSHDMLL PPPADVLEIV RHDIAAMQAL LPDHERTRLA GIGVAQPYNL GAWMRELGLA PDTFRAWEDV DFASDLGRMS SLPVFGENDG NAAAIAELFY GYGRQCDDFV YLFIGPAIGG GIAIDGDCLR GVTGNAGDIA VIPVPPSRLA SAPPPRGPWD ILLARASLHA LVRHLRHHGE RVDNRADLEA CIARGLPAVA EWIDDCVDAL APALRAVLCV VDAPVVVLDA DTDAGLLDAL TTRLRAALVA TAPEARGTPT LVRGTFGADA GAIGAATLPM YFNFSPRAGI LKGARTESQE VNHVAI

NT Sequence

ATGAGAAGTC CGCACATCGG CCAGGGCAGC AATTCGGCCA ACGTGCGCCG TTACAACGAG CGCCTGCTGC TGAAAACGCT GCGCCGCGCC GGCAGCGCGT CGAAGGCCGA CCTCGCCCGC CTCGCGAACA TGACGGGCAC GGCGGTCGGC AGCATCATCG CGTCGCTCGC GGACGCCAAG CTGATCGAAT TCGCGGGCCG CCGCACCGAA GGTCAACGCG GCCAGCCGGC GTCGCTGATC CGTCTCGATC CGCGCGGCGC GTTCGGCATC GGCGTTCATC TCGACCGCAT GCGAATCGAA ACCGCGCTCG TCAATTTCGC CGGCGACGTG CTCGGCCGCC GCTCGCACGA CATGCTGCTG CCCCCGCCCG CCGACGTGCT CGAAATCGTC CGTCACGACA TCGCGGCGAT GCAGGCGCTG CTCCCCGATC ACGAACGTAC GCGGCTCGCC GGCATCGGCG TCGCGCAGCC GTACAACCTC GGCGCCTGGA TGCGCGAACT CGGCCTCGCG CCCGACACGT TCCGCGCCTG GGAAGACGTC GATTTCGCGA GCGACCTCGG GCGCATGTCG TCGCTGCCCG TGTTCGGCGA AAACGACGGC AACGCGGCCG CAATCGCCGA GCTGTTCTAC GGCTACGGCC GCCAGTGCGA CGATTTCGTC TATCTGTTCA TCGGCCCCGC GATCGGCGGC GGCATCGCGA TCGACGGCGA CTGTCTGCGC GGCGTGACCG GCAACGCGGG CGACATCGCG GTCATTCCGG TGCCGCCGAG CCGGCTCGCG TCCGCGCCGC CGCCGCGCGG CCCGTGGGAC ATCCTGCTCG CGCGCGCGTC GCTGCATGCG CTCGTGCGTC ATCTGCGCCA TCACGGCGAA CGCGTCGACA ACCGCGCCGA TCTCGAAGCG TGCATCGCAC GCGGGCTGCC CGCCGTCGCC GAATGGATCG ACGACTGCGT CGACGCGCTC GCCCCCGCGT TGCGCGCGGT GCTGTGCGTC GTCGATGCGC CGGTCGTCGT GCTCGACGCC GATACCGACG CCGGCCTGCT CGATGCGCTG ACGACGCGGC TGCGCGCGGC GCTCGTCGCG ACCGCGCCCG AAGCGCGCGG CACGCCGACG CTCGTGCGCG GCACGTTCGG CGCGGACGCC GGCGCGATCG GCGCCGCGAC GCTGCCGATG TACTTCAACT TCTCCCCGCG GGCCGGCATC CTCAAAGGCG CCCGCACGGA ATCGCAGGAG GTCAACCATG TCGCGATC

Details for BumuA.12797.a.B1.GE39715

HARVESTED ON:	12/3/2015
SEQUENCED ON:	12/7/2015
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR SPHIGQGSNS ANVRRYNERL LLKTLRRAGS ASKADLARLA NMTGTAVGSI IASLADAKLI EFAGRRTEGQ RGQPASLIRL DPRGAFGIGV HLDRMRIETA LVNFAGDVLG RRSHDMLLPP PADVLEIVRH DIAAMQALLP DHERTRLAGI GVAQPYNLGA WMRELGLAPD TFRAWEDVDF ASDLGRMSSL PVFGENDGNA AAIAELFYGY GRQCDDFVYL FIGPAIGGGI AIDGDCLRGV TGNAGDIAVI PVPPSRLASA PPPRGPWDIL LARASLHALV RHLRHHGERV DNRADLEACI ARGLPAVAEW IDDCVDALAP ALRAVLCVVD APVVVLDADT DAGLLDALTT RLRAALVATA PEARGTPTLV RGTFGADAGA IGAATLPMYF NFSPRAGILK GARTXXXXVN HVAI

Validated NT Sequence

atggctcacc accaccacca ccatatgaga agtccgcaca tcggccaggg cagcaattcg gccaacgtgc gccgttacaa cgagcgcctg ctgctgaaaa cgctgcgccg cgccggcagc gcgtcgaagg ccgacctcgc ccgcctcgcg aacatgacgg gcacggcggt cggcagcatc atcgcgtcgc tcgcggacgc caagctgatc gaattcgcgg gccgccgcac cgaaggtcaa cgcggccagc cggcgtcgct gatccgtctc gatccgcgcg gcgcgttcgg catcggcgtt catctcgacc gcatgcgaat cgaaaccgcg ctcgtcaatt tcgccgg-cg acgtgctcgg ccgccgctcg cacgacatgc tgctgccccc gcccgccgac gtgctcgaaa tcgtccgtca cgacatcgcg gcgatgcagg cgctgctccc cgatcacgaa cgtacgcggc tcgccggcat cggcgtcgcg carccgtaca acctcggcgc ctggatgcgc gaactcggcc tcgcgcccga cacgttccgc gcctgggaag acgtcgattt cgcgagcgac ctcgggcgca tgtcgtcgct gcccgtgttc ggcgaaaacg acggcaacgc gg-ccgcaat cgccgagctg ttctacggct acggccgcca gtgcgacgat ttcgtctatc tgttcatcgg ccccgcgatc ggcggcggca tcgcgatcga cggcgactgt ctgcgcggcg tgaccggcaa cgcgggcgac atcgcggtca ttccggtgcc gccgagccgg ctcgcgtccg cgccgccgcc gcgcggcccg tgggacatcc tgctcgcgcg cgcgtcgctg catgcgctcg tgcgtcatct gcgccatcac ggcgaacgcg tcgacaaccg cgccgatctc gaagcgtgca tcgcacgcgg gctgcccgcc gtcgccgaat ggatcgacga ctgcgtcgac gcgctcgccc ccgcgttgcg cgcggtgctg tgcgtcgtcg atgcgccggt cgtcgtgctc gacgccgata ccgacgccgg cctgctcgat gcgctgacga cgcggctgcg cgcggcgctc gtcgcgaccg cgcccgaagc gcgcggcacg ccgacgctcg tgcgcggcac gttcggcgcg gacgccggcg cgatcggcgc cgcgacgctg ccgatgtact tcaacttctc cccgcgggcc ggcatcctca aaggcgcccg cacgganncg nngnnggtca accatgtcgc gatctaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagaa gtccgcacat cggccagggc agcaattcgg ccaacgtgcg ccgttacaac gagcgcctgc tgctgaaaac gctgcgccgc gccggcagcg cgtcgaaggc cgacctcgcc cgcctcgcga acatgacggg cacggcggtc ggcagcatca tcgcgtcgct cgcggacgcc aagctgatcg aattcgcggg ccgccgcacc gaaggtcaac gcggccagcc ggcgtcgctg atccgtctcg atccgcgcgg cgcgttcggc atcggcgttc atctcgaccg catgcgaatc gaaaccgcgc tcgtcaattt cgccggcgac gtgctcggcc gccgctcgca cgacatgctg ctgcccccgc ccgccgacgt gctcgaaatc gtccgtcacg acatcgcggc gatgcaggcg ctgctccccg atcacgaacg tacgcggctc gccggcatcg gcgtcgcgca gccgtacaac ctcggcgcct ggatgcgcga actcggcctc gcgcccgaca cgttccgcgc ctgggaagac gtcgatttcg cgagcgacct cgggcgcatg tcgtcgctgc ccgtgttcgg cgaaaacgac ggcaacgcgg ccgcaatcgc cgagctgttc tacggctacg gccgccagtg cgacgatttc gtctatctgt tcatcggccc cgcgatcggc ggcggcatcg cgatcgacgg cgactgtctg cgcggcgtga ccggcaacgc gggcgacatc gcggtcattc cggtgccgcc gagccggctc gcgtccgcgc cgccgccgcg cggcccgtgg gacatcctgc tcgcgcgcgc gtcgctgcat gcgctcgtgc gtcatctgcg ccatcacggc gaacgcgtcg acaaccgcgc cgatctcgaa gcgtgcatcg cacgcgggct gcccgccgtc gccgaatgga tcgacgactg cgtcgacgcg ctcgcccccg cgttgcgcgc ggtgctgtgc gtcgtcgatg cgccggtcgt cgtgctcgac gccgataccg acgccggcct gctcgatgcg ctgacgacgc ggctgcgcgc ggcgctcgtc gcgaccgcgc ccgaagcgcg cggcacgccg acgctcgtgc gcggcacgtt cggcgcggac gccggcgcga tcggcgccgc gacgctgccg atgtacttca acttctcccc gcgggccggc atcctcaaag gcgcccgcac ggaatcgcag gaggtcaacc atgtcgcgat ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat