BumuA.12245.e
Amidase, hydantoinase/carbamoylase family
| CENTER ID: | BumuA.12245.e |
| ORGANISM: | Burkholderia multivorans ATCC 17616 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.12245.e.B1.GE39712 | Full length( BumuA.12245.e ) | 1 | 426 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.12245.e.B1.PW37879 | Full length( BumuA.12245.e ) | 1 | 426 |
Structures
5THW
DEPOSITED: 9/30/2016
DETERMINATION: XRay
CLONE: BumuA.12245.e.B1.GE39712
PROTEIN: BumuA.12245.e.B1.PW37879
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130721 |
| PATRIC ID: | fig|395019.8.peg.5694 |
| RefSeq: | YP_001585311.1 |
| UniProt: | A0A0H3KRF1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNAVSEALKR TAIDPSIKVD GKRLWNSLME MAKIGATPKG GVCRLALTDL DKAGRDLIVR WAKEAGCTVT VDTMGNVFMR RAGRVADAAP VVTGSHADSQ PTGGRFDGIY GVLGGLEVIR SLNDHGIETE HPIEVVIWTN EEGSRFAPAM VASGVFAGVF TLEYGLSRKD VDGKTIGEEL QRIGYAGDVP CGGRPLHAAF ELHIEQGPIL EAERKTIGVV TDAQGQRWYE ITFTGQEAHA GPTPMPRRRD ALLGASRVVD LVNRIGLDHA PFGCATVGMM QVHPNSRNVI PGRVFFTVDF RHPDDAVLAQ MDAALRDGVA RIAADIGLET ALEQIFYYKP VAFDPACVQA VREAAERFGY PHRDIVSGAG HDACYLAQVA PTSMVFVPCV DGISHNEVED ATPEWIEAGA NVLLHAMLSR ACEPVS
NT Sequence
ATGAATGCGG TATCGGAAGC ATTGAAGCGG ACGGCCATCG ATCCGTCGAT CAAGGTAGAC GGCAAGCGTT TGTGGAACAG CCTGATGGAA ATGGCGAAGA TCGGCGCGAC GCCGAAGGGC GGCGTCTGCC GCCTCGCGCT GACCGATCTC GACAAGGCGG GCCGCGACCT GATCGTTCGC TGGGCGAAGG AAGCCGGGTG CACGGTGACC GTCGATACGA TGGGCAACGT GTTCATGCGC CGTGCCGGGC GCGTCGCCGA TGCGGCGCCG GTCGTTACGG GCTCGCACGC GGATTCGCAG CCGACCGGCG GCCGCTTCGA CGGCATCTAT GGCGTGCTCG GCGGTCTCGA AGTGATTCGG AGTCTGAACG ACCACGGAAT CGAGACCGAG CATCCGATCG AGGTCGTGAT CTGGACCAAC GAGGAAGGCT CGCGCTTCGC GCCTGCCATG GTCGCGTCGG GCGTGTTCGC GGGCGTGTTT ACGCTCGAGT ACGGGCTGTC GCGCAAGGAC GTCGACGGCA AGACGATCGG CGAGGAACTC CAGCGCATCG GCTATGCGGG CGACGTACCG TGCGGCGGCC GGCCGTTGCA TGCGGCGTTC GAGCTGCATA TCGAACAAGG GCCGATTCTC GAGGCCGAGC GCAAGACGAT CGGTGTCGTC ACCGATGCGC AAGGGCAGCG CTGGTACGAG ATCACGTTCA CCGGACAGGA AGCGCATGCC GGCCCCACGC CGATGCCGCG CCGTCGCGAT GCGCTGCTCG GCGCGTCGCG TGTCGTCGAT CTCGTCAACC GGATCGGCCT CGATCACGCG CCGTTCGGCT GTGCGACGGT CGGGATGATG CAGGTGCATC CGAACTCGCG CAACGTGATT CCGGGCCGTG TGTTCTTCAC CGTCGATTTC CGTCATCCGG ACGATGCGGT GCTCGCGCAG ATGGATGCGG CGCTGCGCGA CGGCGTCGCG CGCATCGCGG CCGACATCGG CCTCGAGACG GCGCTCGAGC AGATCTTCTA CTACAAGCCG GTCGCATTCG ATCCGGCGTG CGTGCAGGCC GTGCGCGAGG CCGCCGAGCG CTTCGGCTAT CCGCATCGCG ACATCGTCTC GGGCGCCGGC CACGATGCGT GCTATCTCGC GCAGGTCGCG CCGACGTCGA TGGTGTTCGT GCCGTGCGTC GACGGCATCA GTCACAACGA GGTCGAAGAC GCGACGCCCG AATGGATCGA GGCCGGCGCG AACGTATTGC TGCACGCGAT GCTGTCGCGC GCGTGCGAGC CGGTGTCA
Details for BumuA.12245.e.B1.GE39712
| HARVESTED ON: | 12/3/2015 |
| SEQUENCED ON: | 12/7/2015 |
| EXPECTED MW: | 47kDa |
| OBSERVED MW: | 47kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMN AVSEALKRTA IDPSIKVDGK RLWNSLMEMA KIGATPKGGV CRLALTDLDK AGRDLIVRWA KEAGCTVTVD TMGNVFMRRA GRVADAAPVV TGSHADSQPT GGRFDGIYGV LGGLEVIRSL NDHGIETEHP IEVVIWTNEE GSRFAPAMVA SGVFAGVFTL EYGLSRKDVD GKTIGEELQR IGYAGDVPCG GRPLHAAFEL HIEQGPILEA ERKTIGVVTD AQGQRWYEIT FTGQEAHAGP TPMPRRRDAL LGASRVVDLV NRIGLDHAPF GCATVGMMQV HPNSRNVIPG RVFFTVDFRH PDDAVLAQMD AALRDGVARI AADIGLETAL EQIFYYKPVA FDPACVQAVR EAAERFGYPH RDIVSGAGHD ACYLAQVAPT SMVFVPCVDG ISHNEVEDAT PEWIEAGANV LLH
Validated NT Sequence
cgtgcagcaa tacgttcgcg ccggcctcga tccattcggg cgtcgcgtct tcgacctcgt tgtgactgat gccgtcgacg cacggcacga acaccatcga cgtcggcgcg acctgcgcga gatagcacgc atcgtggccg gcgcccgaga cgatgtcgcg atgcggatag ccgaagcgct cggcggcctc gcgcacggcc tgcacgcacg ccggatcgaa tgcgaccggc ttgtagtaga agatctgctc gagcgccgtc tcgaggccga tgtcggccgc gatgcgcgcg acgccgtcgc gcagcgccgc atccatctgc gcgagcaccg catcgtccgg atgacggaaa tcgacggtga agaacacacg gcccggaatc acgttgcgcg agttcggatg cacctgcatc atcccgaccg tcgcacagcc gaacggcgcg tgatcgaggc cgatccggtt gacgagatcg acgacacgcg acgcgccgag cagcgcatcg cgacggcgcg gcatcggcgt ggggccggca tgcgcttcct gtccggtgaa cgtgatctcg taccagcgct gcccttgcgc atcggtgacg acaccgatcg tcttgcgctc ggcctcgaga atcggccctt gttcgatatg cagctcgaac gccgcatgca acggccggcc gccgcacggt acgtcgcccg catagccgat gcgctggagt tcctcgccga tcgtcttgcc gtcgacgtcc ttgcgcgaca gcccgtactc gagcgtaaac acgcccgcga acacgcccga cgcgaccatg gcaggcgcga agcgcgagcc ttcctcgttg gtccagatca cgacctcgat cggatgctcg gtctcgattc cgtggtcgtt cagactccga atcacttcga gaccgccgag cacgccatag atgccgtcga agcggccgcc ggtcggctgc gaatccgcgt gcgagcccgt aacgaccggc gccgcatcgg cgacgcgccc ggcacggcgc atgaacacgt tgcccatcgt atcgacggtc accgtgcacc cggcttcctt cgcccagcga acgatcaggt cgcggcccgc cttgtcgaga tcggtcagcg cgaggcggca gacgccgccc ttcggcgtcg cgccgatctt cgccatttcc atcaggctgt tccacaaacg cttgccgtct accttgatcg acggatcgat ggccgtccgc ttcaatgctt ccgataccgc attcatatgg tggtggtggt ggtgagccat ggtata
Expected Protein Sequence
MAHHHHHHMN AVSEALKRTA IDPSIKVDGK RLWNSLMEMA KIGATPKGGV CRLALTDLDK AGRDLIVRWA KEAGCTVTVD TMGNVFMRRA GRVADAAPVV TGSHADSQPT GGRFDGIYGV LGGLEVIRSL NDHGIETEHP IEVVIWTNEE GSRFAPAMVA SGVFAGVFTL EYGLSRKDVD GKTIGEELQR IGYAGDVPCG GRPLHAAFEL HIEQGPILEA ERKTIGVVTD AQGQRWYEIT FTGQEAHAGP TPMPRRRDAL LGASRVVDLV NRIGLDHAPF GCATVGMMQV HPNSRNVIPG RVFFTVDFRH PDDAVLAQMD AALRDGVARI AADIGLETAL EQIFYYKPVA FDPACVQAVR EAAERFGYPH RDIVSGAGHD ACYLAQVAPT SMVFVPCVDG ISHNEVEDAT PEWIEAGANV LLHAMLSRAC EPVS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg cggtatcgga agcattgaag cggacggcca tcgatccgtc gatcaaggta gacggcaagc gtttgtggaa cagcctgatg gaaatggcga agatcggcgc gacgccgaag ggcggcgtct gccgcctcgc gctgaccgat ctcgacaagg cgggccgcga cctgatcgtt cgctgggcga aggaagccgg gtgcacggtg accgtcgata cgatgggcaa cgtgttcatg cgccgtgccg ggcgcgtcgc cgatgcggcg ccggtcgtta cgggctcgca cgcggattcg cagccgaccg gcggccgctt cgacggcatc tatggcgtgc tcggcggtct cgaagtgatt cggagtctga acgaccacgg aatcgagacc gagcatccga tcgaggtcgt gatctggacc aacgaggaag gctcgcgctt cgcgcctgcc atggtcgcgt cgggcgtgtt cgcgggcgtg tttacgctcg agtacgggct gtcgcgcaag gacgtcgacg gcaagacgat cggcgaggaa ctccagcgca tcggctatgc gggcgacgta ccgtgcggcg gccggccgtt gcatgcggcg ttcgagctgc atatcgaaca agggccgatt ctcgaggccg agcgcaagac gatcggtgtc gtcaccgatg cgcaagggca gcgctggtac gagatcacgt tcaccggaca ggaagcgcat gccggcccca cgccgatgcc gcgccgtcgc gatgcgctgc tcggcgcgtc gcgtgtcgtc gatctcgtca accggatcgg cctcgatcac gcgccgttcg gctgtgcgac ggtcgggatg atgcaggtgc atccgaactc gcgcaacgtg attccgggcc gtgtgttctt caccgtcgat ttccgtcatc cggacgatgc ggtgctcgcg cagatggatg cggcgctgcg cgacggcgtc gcgcgcatcg cggccgacat cggcctcgag acggcgctcg agcagatctt ctactacaag ccggtcgcat tcgatccggc gtgcgtgcag gccgtgcgcg aggccgccga gcgcttcggc tatccgcatc gcgacatcgt ctcgggcgcc ggccacgatg cgtgctatct cgcgcaggtc gcgccgacgt cgatggtgtt cgtgccgtgc gtcgacggca tcagtcacaa cgaggtcgaa gacgcgacgc ccgaatggat cgaggccggc gcgaacgtat tgctgcacgc gatgctgtcg cgcgcgtgcg agccggtgtc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BumuA.12245.e.B1.PW37879
| PURIFICATION DATe: | 3/23/2016 |
| CONCENTRATION: | 39mg/ml |
| OBSERVED MW: | 46kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 18 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN AVSEALKRTA IDPSIKVDGK RLWNSLMEMA KIGATPKGGV CRLALTDLDK AGRDLIVRWA KEAGCTVTVD TMGNVFMRRA GRVADAAPVV TGSHADSQPT GGRFDGIYGV LGGLEVIRSL NDHGIETEHP IEVVIWTNEE GSRFAPAMVA SGVFAGVFTL EYGLSRKDVD GKTIGEELQR IGYAGDVPCG GRPLHAAFEL HIEQGPILEA ERKTIGVVTD AQGQRWYEIT FTGQEAHAGP TPMPRRRDAL LGASRVVDLV NRIGLDHAPF GCATVGMMQV HPNSRNVIPG RVFFTVDFRH PDDAVLAQMD AALRDGVARI AADIGLETAL EQIFYYKPVA FDPACVQAVR EAAERFGYPH RDIVSGAGHD ACYLAQVAPT SMVFVPCVDG ISHNEVEDAT PEWIEAGANV LLH
Validated NT Sequence
cgtgcagcaa tacgttcgcg ccggcctcga tccattcggg cgtcgcgtct tcgacctcgt tgtgactgat gccgtcgacg cacggcacga acaccatcga cgtcggcgcg acctgcgcga gatagcacgc atcgtggccg gcgcccgaga cgatgtcgcg atgcggatag ccgaagcgct cggcggcctc gcgcacggcc tgcacgcacg ccggatcgaa tgcgaccggc ttgtagtaga agatctgctc gagcgccgtc tcgaggccga tgtcggccgc gatgcgcgcg acgccgtcgc gcagcgccgc atccatctgc gcgagcaccg catcgtccgg atgacggaaa tcgacggtga agaacacacg gcccggaatc acgttgcgcg agttcggatg cacctgcatc atcccgaccg tcgcacagcc gaacggcgcg tgatcgaggc cgatccggtt gacgagatcg acgacacgcg acgcgccgag cagcgcatcg cgacggcgcg gcatcggcgt ggggccggca tgcgcttcct gtccggtgaa cgtgatctcg taccagcgct gcccttgcgc atcggtgacg acaccgatcg tcttgcgctc ggcctcgaga atcggccctt gttcgatatg cagctcgaac gccgcatgca acggccggcc gccgcacggt acgtcgcccg catagccgat gcgctggagt tcctcgccga tcgtcttgcc gtcgacgtcc ttgcgcgaca gcccgtactc gagcgtaaac acgcccgcga acacgcccga cgcgaccatg gcaggcgcga agcgcgagcc ttcctcgttg gtccagatca cgacctcgat cggatgctcg gtctcgattc cgtggtcgtt cagactccga atcacttcga gaccgccgag cacgccatag atgccgtcga agcggccgcc ggtcggctgc gaatccgcgt gcgagcccgt aacgaccggc gccgcatcgg cgacgcgccc ggcacggcgc atgaacacgt tgcccatcgt atcgacggtc accgtgcacc cggcttcctt cgcccagcga acgatcaggt cgcggcccgc cttgtcgaga tcggtcagcg cgaggcggca gacgccgccc ttcggcgtcg cgccgatctt cgccatttcc atcaggctgt tccacaaacg cttgccgtct accttgatcg acggatcgat ggccgtccgc ttcaatgctt ccgataccgc attcatatgg tggtggtggt ggtgagccat ggtata
Expressed Protein Sequence
MAHHHHHHMN AVSEALKRTA IDPSIKVDGK RLWNSLMEMA KIGATPKGGV CRLALTDLDK AGRDLIVRWA KEAGCTVTVD TMGNVFMRRA GRVADAAPVV TGSHADSQPT GGRFDGIYGV LGGLEVIRSL NDHGIETEHP IEVVIWTNEE GSRFAPAMVA SGVFAGVFTL EYGLSRKDVD GKTIGEELQR IGYAGDVPCG GRPLHAAFEL HIEQGPILEA ERKTIGVVTD AQGQRWYEIT FTGQEAHAGP TPMPRRRDAL LGASRVVDLV NRIGLDHAPF GCATVGMMQV HPNSRNVIPG RVFFTVDFRH PDDAVLAQMD AALRDGVARI AADIGLETAL EQIFYYKPVA FDPACVQAVR EAAERFGYPH RDIVSGAGHD ACYLAQVAPT SMVFVPCVDG ISHNEVEDAT PEWIEAGANV LLHAMLSRAC EPVS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATG CGGTATCGGA AGCATTGAAG CGGACGGCCA TCGATCCGTC GATCAAGGTA GACGGCAAGC GTTTGTGGAA CAGCCTGATG GAAATGGCGA AGATCGGCGC GACGCCGAAG GGCGGCGTCT GCCGCCTCGC GCTGACCGAT CTCGACAAGG CGGGCCGCGA CCTGATCGTT CGCTGGGCGA AGGAAGCCGG GTGCACGGTG ACCGTCGATA CGATGGGCAA CGTGTTCATG CGCCGTGCCG GGCGCGTCGC CGATGCGGCG CCGGTCGTTA CGGGCTCGCA CGCGGATTCG CAGCCGACCG GCGGCCGCTT CGACGGCATC TATGGCGTGC TCGGCGGTCT CGAAGTGATT CGGAGTCTGA ACGACCACGG AATCGAGACC GAGCATCCGA TCGAGGTCGT GATCTGGACC AACGAGGAAG GCTCGCGCTT CGCGCCTGCC ATGGTCGCGT CGGGCGTGTT CGCGGGCGTG TTTACGCTCG AGTACGGGCT GTCGCGCAAG GACGTCGACG GCAAGACGAT CGGCGAGGAA CTCCAGCGCA TCGGCTATGC GGGCGACGTA CCGTGCGGCG GCCGGCCGTT GCATGCGGCG TTCGAGCTGC ATATCGAACA AGGGCCGATT CTCGAGGCCG AGCGCAAGAC GATCGGTGTC GTCACCGATG CGCAAGGGCA GCGCTGGTAC GAGATCACGT TCACCGGACA GGAAGCGCAT GCCGGCCCCA CGCCGATGCC GCGCCGTCGC GATGCGCTGC TCGGCGCGTC GCGTGTCGTC GATCTCGTCA ACCGGATCGG CCTCGATCAC GCGCCGTTCG GCTGTGCGAC GGTCGGGATG ATGCAGGTGC ATCCGAACTC GCGCAACGTG ATTCCGGGCC GTGTGTTCTT CACCGTCGAT TTCCGTCATC CGGACGATGC GGTGCTCGCG CAGATGGATG CGGCGCTGCG CGACGGCGTC GCGCGCATCG CGGCCGACAT CGGCCTCGAG ACGGCGCTCG AGCAGATCTT CTACTACAAG CCGGTCGCAT TCGATCCGGC GTGCGTGCAG GCCGTGCGCG AGGCCGCCGA GCGCTTCGGC TATCCGCATC GCGACATCGT CTCGGGCGCC GGCCACGATG CGTGCTATCT CGCGCAGGTC GCGCCGACGT CGATGGTGTT CGTGCCGTGC GTCGACGGCA TCAGTCACAA CGAGGTCGAA GACGCGACGC CCGAATGGAT CGAGGCCGGC GCGAACGTAT TGCTGCACGC GATGCTGTCG CGCGCGTGCG AGCCGGTGTC ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT