BumuA.12245.c

Amidase, hydantoinase/carbamoylase family

CENTER ID: BumuA.12245.c
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.12245.c.B1.GE39710 Full length( BumuA.12245.c ) 1 414
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.12245.c.B1.PW37880 Full length( BumuA.12245.c ) 1 414

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.4248
RefSeq: YP_001583939.1
UniProt: A0A0H3KVJ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTSLQLDGA VLLRQLRELG EIGADRELGG RTRIALTDDE RAGRDRVVAW MRELDLDVRI DRIGNVFGTL HAGPAAPGRQ PLMIGSHIDT VKNAGALDGC YGVLAGLAVA RAFRDARIEP ARPITVAAFT NEEGIRYQPD MMGSLVHAGG LSIDDALNTI GIDGTRLGDE LARIGYAGDL EPGAIVPHAY LELHIEQGPI LEAENVRIGV VENLQGISWQ QITVQGNANH AGTTPMHLRH DAGWVAAAIA TFLRELAVSS GTTLATIGML RIEPNVINVI PRKAVLTVDL RDPDEQRLQQ AEQRLADHLE QLAALEGVQI STERLARFEP VVFDAALVDA IEKAAARRGF SYRRMTSGAG HDAQMIARIA PAAMIFVPSR GGISHNPREH TDDSQLVDGA RLLLDVVLDR LAAA
NT Sequence
ATGAACACGT CGCTGCAACT GGACGGCGCA GTCCTTCTGC GTCAACTGCG CGAGCTCGGC GAGATCGGCG CCGACCGCGA ACTCGGCGGC CGCACGCGCA TCGCGCTGAC CGACGACGAG CGCGCGGGCC GCGACCGCGT CGTCGCGTGG ATGCGTGAGC TGGACCTCGA CGTCCGGATC GACCGCATCG GCAACGTCTT CGGCACGCTG CACGCCGGCC CGGCGGCCCC GGGCCGGCAG CCGTTGATGA TCGGCTCGCA TATCGACACG GTAAAGAACG CCGGCGCGCT CGACGGCTGC TACGGCGTGC TCGCCGGTCT TGCGGTCGCG CGCGCGTTTC GCGACGCGCG TATCGAACCG GCACGCCCGA TCACGGTCGC CGCCTTTACG AACGAGGAAG GCATCCGCTA TCAGCCCGAC ATGATGGGCT CGCTCGTCCA TGCGGGCGGC CTGTCGATCG ACGACGCGCT GAACACGATC GGCATCGACG GCACGCGCCT CGGCGATGAG CTCGCGCGCA TCGGTTATGC CGGCGACCTC GAACCGGGCG CGATCGTCCC GCATGCGTAT CTGGAGCTGC ATATCGAGCA GGGGCCGATC CTCGAGGCGG AGAACGTCCG TATCGGCGTC GTCGAGAACC TGCAGGGTAT CTCGTGGCAG CAGATCACCG TACAGGGGAA CGCGAACCAT GCAGGCACGA CGCCCATGCA TCTGCGCCAC GATGCGGGCT GGGTCGCGGC CGCGATCGCG ACGTTTCTAC GCGAACTCGC GGTGTCGTCC GGTACGACGC TCGCGACGAT CGGCATGCTG CGCATCGAGC CGAACGTGAT CAACGTTATC CCACGCAAGG CCGTGTTGAC GGTGGACCTG CGCGACCCTG ACGAGCAGCG GCTGCAGCAG GCGGAGCAGC GTCTCGCCGA CCATCTCGAG CAGCTCGCGG CACTCGAAGG CGTGCAGATT TCGACCGAGC GGCTCGCGCG TTTCGAGCCG GTCGTGTTCG ATGCCGCGCT CGTCGACGCA ATCGAAAAAG CCGCTGCGCG CCGTGGCTTT TCGTATCGAC GAATGACGTC CGGCGCCGGC CACGACGCGC AGATGATCGC GCGCATCGCG CCGGCCGCCA TGATCTTCGT GCCGAGCCGC GGCGGCATCA GCCACAACCC GCGCGAACAT ACGGACGACA GCCAGCTCGT CGACGGCGCC CGTCTGCTGC TCGACGTCGT GCTCGATCGT CTGGCGGCCG CG
Details for BumuA.12245.c.B1.GE39710
HARVESTED ON: 12/3/2015
SEQUENCED ON: 12/7/2015
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMN TSLQLDGAVL LRQLRELGEI GADRELGGRT RIALTDDERA GRDRVVAWMR ELDLDVRIDR IGNVFGTLHA GPAAPGRQPL MIGSHIDTVK NAGALDGCYG VLAGLAVARA FRDARIEPAR PITVAAFTNE EGIRYQPDMM GSLVHAGGLS IDDALNTIGI DGTRLGDELA RIGYAGDLEP GAIVPHAYLE LHIEQGPILE AENVRIGVVE NLQGISWQQI TVQGNANHAG TTPMHLRHDA GWVAAAIATF LRELAVSSGT TLATIGMLRI EPNVINVIPR KAVLTVDLRD PDEQRLQQAE QRLADHLEQL AALEGVQIST ERLARFEPVV FDAALVDAIE KAAARRGFSY RRMTSGAGHD AQMIARIAPA AMIFVPSRGG ISHNPREHTD DSQLVDGARL L
Validated NT Sequence
agcagcagac gggcgccgtc gacgagctgg ctgtcgtccg tatgttcgcg cgggttgtgg ctgatgccgc cgcggctcgg cacgaagatc atggcggccg gcgcgatgcg cgcgatcatc tgcgcgtcgt ggccggcgcc ggacgtcatt cgtcgatacg aaaagccacg gcgcgcagcg gctttttcga ttgcgtcgac gagcgcggca tcgaacacga ccggctcgaa acgcgcgagc cgctcggtcg aaatctgcac gccttcgagt gccgcgagct gctcgagatg gtcggcgaga cgctgctccg cctgctgcag ccgctgctcg tcagggtcgc gcaggtccac cgtcaacacg gccttgcgtg ggataacgtt gatcacgttc ggctcgatgc gcagcatgcc gatcgtcgcg agcgtcgtac cggacgacac cgcgagttcg cgtagaaacg tcgcgatcgc ggccgcgacc cagcccgcat cgtggcgcag atgcatgggc gtcgtgcctg catggttcgc gttcccctgt acggtgatct gctgccacga gataccctgc aggttctcga cgacgccgat acggacgttc tccgcctcga ggatcggccc ctgctcgata tgcagctcca gatacgcatg cgggacgatc gcgcccggtt cgaggtcgcc ggcataaccg atgcgcgcga gctcatcgcc gaggcgcgtg ccgtcgatgc cgatcgtgtt cagcgcgtcg tcgatcgaca ggccgcccgc atggacgagc gagcccatca tgtcgggctg atagcggatg ccttcctcgt tcgtaaaggc ggcgaccgtg atcgggcgtg ccggttcgat acgcgcgtcg cgaaacgcgc gcgcgaccgc aagaccggcg agcacgccgt agcagccgtc gagcgcgccg gcgttcttta ccgtgtcgat atgcgagccg atcatcaacg gctgccggcc cggggccgcc gggccggcgt gcagcgtgcc gaagacgttg ccgatgcggt cgatccggac gtcgaggtcc agctcacgca tccacgcgac gacgcggtcg cggcccgcgc gctcgtcgtc ggtcagcgcg atgcgcgtgc ggccgccgag ttcgcggtcg gcgccgatct cgccgagctc gcgcagttga cgcagaagga ctgcgccgtc cagttgcagc gacgtgttca tatggtggtg gtggtggtga gccatgg
Expected Protein Sequence
MAHHHHHHMN TSLQLDGAVL LRQLRELGEI GADRELGGRT RIALTDDERA GRDRVVAWMR ELDLDVRIDR IGNVFGTLHA GPAAPGRQPL MIGSHIDTVK NAGALDGCYG VLAGLAVARA FRDARIEPAR PITVAAFTNE EGIRYQPDMM GSLVHAGGLS IDDALNTIGI DGTRLGDELA RIGYAGDLEP GAIVPHAYLE LHIEQGPILE AENVRIGVVE NLQGISWQQI TVQGNANHAG TTPMHLRHDA GWVAAAIATF LRELAVSSGT TLATIGMLRI EPNVINVIPR KAVLTVDLRD PDEQRLQQAE QRLADHLEQL AALEGVQIST ERLARFEPVV FDAALVDAIE KAAARRGFSY RRMTSGAGHD AQMIARIAPA AMIFVPSRGG ISHNPREHTD DSQLVDGARL LLDVVLDRLA AA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca cgtcgctgca actggacggc gcagtccttc tgcgtcaact gcgcgagctc ggcgagatcg gcgccgaccg cgaactcggc ggccgcacgc gcatcgcgct gaccgacgac gagcgcgcgg gccgcgaccg cgtcgtcgcg tggatgcgtg agctggacct cgacgtccgg atcgaccgca tcggcaacgt cttcggcacg ctgcacgccg gcccggcggc cccgggccgg cagccgttga tgatcggctc gcatatcgac acggtaaaga acgccggcgc gctcgacggc tgctacggcg tgctcgccgg tcttgcggtc gcgcgcgcgt ttcgcgacgc gcgtatcgaa ccggcacgcc cgatcacggt cgccgccttt acgaacgagg aaggcatccg ctatcagccc gacatgatgg gctcgctcgt ccatgcgggc ggcctgtcga tcgacgacgc gctgaacacg atcggcatcg acggcacgcg cctcggcgat gagctcgcgc gcatcggtta tgccggcgac ctcgaaccgg gcgcgatcgt cccgcatgcg tatctggagc tgcatatcga gcaggggccg atcctcgagg cggagaacgt ccgtatcggc gtcgtcgaga acctgcaggg tatctcgtgg cagcagatca ccgtacaggg gaacgcgaac catgcaggca cgacgcccat gcatctgcgc cacgatgcgg gctgggtcgc ggccgcgatc gcgacgtttc tacgcgaact cgcggtgtcg tccggtacga cgctcgcgac gatcggcatg ctgcgcatcg agccgaacgt gatcaacgtt atcccacgca aggccgtgtt gacggtggac ctgcgcgacc ctgacgagca gcggctgcag caggcggagc agcgtctcgc cgaccatctc gagcagctcg cggcactcga aggcgtgcag atttcgaccg agcggctcgc gcgtttcgag ccggtcgtgt tcgatgccgc gctcgtcgac gcaatcgaaa aagccgctgc gcgccgtggc ttttcgtatc gacgaatgac gtccggcgcc ggccacgacg cgcagatgat cgcgcgcatc gcgccggccg ccatgatctt cgtgccgagc cgcggcggca tcagccacaa cccgcgcgaa catacggacg acagccagct cgtcgacggc gcccgtctgc tgctcgacgt cgtgctcgat cgtctggcgg ccgcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BumuA.12245.c.B1.PW37880
PURIFICATION DATe: 3/29/2016
CONCENTRATION: 32.5mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 24
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN TSLQLDGAVL LRQLRELGEI GADRELGGRT RIALTDDERA GRDRVVAWMR ELDLDVRIDR IGNVFGTLHA GPAAPGRQPL MIGSHIDTVK NAGALDGCYG VLAGLAVARA FRDARIEPAR PITVAAFTNE EGIRYQPDMM GSLVHAGGLS IDDALNTIGI DGTRLGDELA RIGYAGDLEP GAIVPHAYLE LHIEQGPILE AENVRIGVVE NLQGISWQQI TVQGNANHAG TTPMHLRHDA GWVAAAIATF LRELAVSSGT TLATIGMLRI EPNVINVIPR KAVLTVDLRD PDEQRLQQAE QRLADHLEQL AALEGVQIST ERLARFEPVV FDAALVDAIE KAAARRGFSY RRMTSGAGHD AQMIARIAPA AMIFVPSRGG ISHNPREHTD DSQLVDGARL L
Validated NT Sequence
agcagcagac gggcgccgtc gacgagctgg ctgtcgtccg tatgttcgcg cgggttgtgg ctgatgccgc cgcggctcgg cacgaagatc atggcggccg gcgcgatgcg cgcgatcatc tgcgcgtcgt ggccggcgcc ggacgtcatt cgtcgatacg aaaagccacg gcgcgcagcg gctttttcga ttgcgtcgac gagcgcggca tcgaacacga ccggctcgaa acgcgcgagc cgctcggtcg aaatctgcac gccttcgagt gccgcgagct gctcgagatg gtcggcgaga cgctgctccg cctgctgcag ccgctgctcg tcagggtcgc gcaggtccac cgtcaacacg gccttgcgtg ggataacgtt gatcacgttc ggctcgatgc gcagcatgcc gatcgtcgcg agcgtcgtac cggacgacac cgcgagttcg cgtagaaacg tcgcgatcgc ggccgcgacc cagcccgcat cgtggcgcag atgcatgggc gtcgtgcctg catggttcgc gttcccctgt acggtgatct gctgccacga gataccctgc aggttctcga cgacgccgat acggacgttc tccgcctcga ggatcggccc ctgctcgata tgcagctcca gatacgcatg cgggacgatc gcgcccggtt cgaggtcgcc ggcataaccg atgcgcgcga gctcatcgcc gaggcgcgtg ccgtcgatgc cgatcgtgtt cagcgcgtcg tcgatcgaca ggccgcccgc atggacgagc gagcccatca tgtcgggctg atagcggatg ccttcctcgt tcgtaaaggc ggcgaccgtg atcgggcgtg ccggttcgat acgcgcgtcg cgaaacgcgc gcgcgaccgc aagaccggcg agcacgccgt agcagccgtc gagcgcgccg gcgttcttta ccgtgtcgat atgcgagccg atcatcaacg gctgccggcc cggggccgcc gggccggcgt gcagcgtgcc gaagacgttg ccgatgcggt cgatccggac gtcgaggtcc agctcacgca tccacgcgac gacgcggtcg cggcccgcgc gctcgtcgtc ggtcagcgcg atgcgcgtgc ggccgccgag ttcgcggtcg gcgccgatct cgccgagctc gcgcagttga cgcagaagga ctgcgccgtc cagttgcagc gacgtgttca tatggtggtg gtggtggtga gccatgg
Expressed Protein Sequence
MAHHHHHHMN TSLQLDGAVL LRQLRELGEI GADRELGGRT RIALTDDERA GRDRVVAWMR ELDLDVRIDR IGNVFGTLHA GPAAPGRQPL MIGSHIDTVK NAGALDGCYG VLAGLAVARA FRDARIEPAR PITVAAFTNE EGIRYQPDMM GSLVHAGGLS IDDALNTIGI DGTRLGDELA RIGYAGDLEP GAIVPHAYLE LHIEQGPILE AENVRIGVVE NLQGISWQQI TVQGNANHAG TTPMHLRHDA GWVAAAIATF LRELAVSSGT TLATIGMLRI EPNVINVIPR KAVLTVDLRD PDEQRLQQAE QRLADHLEQL AALEGVQIST ERLARFEPVV FDAALVDAIE KAAARRGFSY RRMTSGAGHD AQMIARIAPA AMIFVPSRGG ISHNPREHTD DSQLVDGARL LLDVVLDRLA AA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACA CGTCGCTGCA ACTGGACGGC GCAGTCCTTC TGCGTCAACT GCGCGAGCTC GGCGAGATCG GCGCCGACCG CGAACTCGGC GGCCGCACGC GCATCGCGCT GACCGACGAC GAGCGCGCGG GCCGCGACCG CGTCGTCGCG TGGATGCGTG AGCTGGACCT CGACGTCCGG ATCGACCGCA TCGGCAACGT CTTCGGCACG CTGCACGCCG GCCCGGCGGC CCCGGGCCGG CAGCCGTTGA TGATCGGCTC GCATATCGAC ACGGTAAAGA ACGCCGGCGC GCTCGACGGC TGCTACGGCG TGCTCGCCGG TCTTGCGGTC GCGCGCGCGT TTCGCGACGC GCGTATCGAA CCGGCACGCC CGATCACGGT CGCCGCCTTT ACGAACGAGG AAGGCATCCG CTATCAGCCC GACATGATGG GCTCGCTCGT CCATGCGGGC GGCCTGTCGA TCGACGACGC GCTGAACACG ATCGGCATCG ACGGCACGCG CCTCGGCGAT GAGCTCGCGC GCATCGGTTA TGCCGGCGAC CTCGAACCGG GCGCGATCGT CCCGCATGCG TATCTGGAGC TGCATATCGA GCAGGGGCCG ATCCTCGAGG CGGAGAACGT CCGTATCGGC GTCGTCGAGA ACCTGCAGGG TATCTCGTGG CAGCAGATCA CCGTACAGGG GAACGCGAAC CATGCAGGCA CGACGCCCAT GCATCTGCGC CACGATGCGG GCTGGGTCGC GGCCGCGATC GCGACGTTTC TACGCGAACT CGCGGTGTCG TCCGGTACGA CGCTCGCGAC GATCGGCATG CTGCGCATCG AGCCGAACGT GATCAACGTT ATCCCACGCA AGGCCGTGTT GACGGTGGAC CTGCGCGACC CTGACGAGCA GCGGCTGCAG CAGGCGGAGC AGCGTCTCGC CGACCATCTC GAGCAGCTCG CGGCACTCGA AGGCGTGCAG ATTTCGACCG AGCGGCTCGC GCGTTTCGAG CCGGTCGTGT TCGATGCCGC GCTCGTCGAC GCAATCGAAA AAGCCGCTGC GCGCCGTGGC TTTTCGTATC GACGAATGAC GTCCGGCGCC GGCCACGACG CGCAGATGAT CGCGCGCATC GCGCCGGCCG CCATGATCTT CGTGCCGAGC CGCGGCGGCA TCAGCCACAA CCCGCGCGAA CATACGGACG ACAGCCAGCT CGTCGACGGC GCCCGTCTGC TGCTCGACGT CGTGCTCGAT CGTCTGGCGG CCGCGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT