SSGCID: Target Page

BumuA.11726.a

N-acetylmuramoyl-L-alanine amidase

CENTER ID:	BumuA.11726.a
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.11726.a.A2.GE33105	Mature protein( BumuA.11726.a )		48	505

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.11726.a.A2.PS01722	Mature protein( BumuA.11726.a )		48	505

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.883
RefSeq:	YP_001578925.1
UniProt:	A0A0H3KLN6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRKMLIKPF RSIESAATAT HNWRRRQILC AGASTLVLGL VAPRLAHASS VLGVRVWPAR DYTRVTIESD QPLQNSQQLL QGPDRLVVDL TGLELDQALR DLVSKIAPND PQIQSVRVGQ YQPHVVRMVF DLKGSVKPQV FTLPPVGTYK YRLVFDLYPA VAPDPLTDLI AQTERKEQAL NDAARAQQMQ PPTALNGPAA APAPSDNSDA FFQRFAQNTP AAPHTPPAAP SAPAKPAVKP PPVIARRDDG DDDADAYKFT APKSGKGGTV RLLTVAIDPG HGGEDPGAIG GAGTYEKHIA LDIAKKLRAK IDGAPNMRAM MTRDADFFVP LNVRVQKARR VGADLFVSIH ADAFTTPSAR GSSVFALSDH GASSAAARWL ANKENASDLI GGINIKTQDA AVSRALFDMS TTAQIRDSLR YGNYVLKEVG SINKLHKGSV EQAGFAVLKA PDIPSILVET AFISNPDEER RLNDDSYRDQ MADAIFRGIK RYFAANPPLA KNRMA

NT Sequence

ATGTCTCGAA AGATGTTGAT CAAACCGTTC CGCTCGATCG AATCGGCGGC CACCGCGACG CACAACTGGC GGCGCCGGCA GATCCTGTGC GCGGGCGCCT CGACGCTGGT GCTCGGCCTC GTCGCGCCCC GGCTCGCGCA CGCGTCGTCG GTGCTCGGCG TGCGCGTGTG GCCCGCACGC GATTACACGC GCGTGACGAT CGAATCCGAT CAGCCGCTGC AGAATAGCCA ACAGCTGCTG CAGGGGCCCG ACCGGCTCGT CGTCGACCTG ACCGGCCTCG AGCTCGACCA GGCGCTGCGC GACCTCGTGT CGAAGATCGC GCCGAACGAT CCGCAGATTC AATCGGTGCG CGTCGGCCAG TATCAGCCGC ACGTCGTGCG GATGGTGTTC GACCTGAAAG GCTCGGTGAA GCCGCAGGTG TTCACGCTGC CGCCGGTCGG CACCTACAAG TATCGGCTCG TGTTCGACCT CTATCCGGCC GTCGCGCCCG ATCCGCTGAC CGACCTGATC GCGCAGACCG AACGCAAGGA GCAGGCGCTG AACGATGCGG CGCGCGCGCA GCAGATGCAG CCGCCGACCG CGCTGAACGG CCCGGCGGCC GCGCCCGCGC CGAGCGACAA CAGCGACGCG TTTTTCCAGC GCTTCGCGCA GAACACGCCG GCCGCGCCGC ACACGCCGCC GGCCGCGCCT TCGGCGCCCG CGAAGCCGGC CGTCAAGCCG CCGCCCGTGA TCGCGCGCCG CGACGACGGC GACGACGACG CCGATGCCTA CAAGTTCACC GCGCCGAAGT CCGGCAAGGG CGGCACCGTG CGGCTGCTGA CCGTCGCGAT CGATCCGGGC CACGGCGGCG AGGATCCGGG CGCGATCGGC GGCGCCGGCA CGTACGAGAA GCACATCGCG CTCGACATCG CGAAGAAGCT GCGCGCGAAG ATCGATGGCG CGCCGAACAT GCGCGCGATG ATGACGCGCG ACGCCGACTT CTTCGTGCCG CTGAACGTGC GCGTGCAAAA GGCGCGCCGC GTCGGCGCCG ACCTGTTCGT GTCGATCCAC GCCGATGCAT TCACGACGCC GTCCGCGCGC GGCTCGTCGG TGTTCGCGCT GTCGGATCAC GGCGCGTCGA GCGCGGCCGC GCGCTGGCTC GCGAACAAGG AGAATGCGTC CGATCTGATC GGCGGGATCA ACATCAAGAC GCAGGACGCG GCGGTGAGCC GCGCGCTGTT CGACATGTCG ACCACGGCGC AGATTCGCGA TTCGCTGCGC TACGGCAACT ACGTGCTGAA GGAAGTCGGC AGCATCAACA AGCTGCACAA GGGCTCGGTC GAGCAGGCGG GATTCGCGGT GCTGAAGGCG CCCGACATTC CGTCGATCCT CGTCGAGACC GCGTTCATCA GCAACCCGGA CGAGGAGCGC CGGCTCAACG ACGACAGCTA TCGCGACCAG ATGGCCGACG CGATCTTCCG CGGCATCAAG CGCTACTTCG CCGCGAATCC GCCGCTCGCG AAGAACCGCA TGGCC

Details for BumuA.11726.a.A2.GE33105

HARVESTED ON:	8/19/2011
SEQUENCED ON:	8/31/2011
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	82
PERCENT COVERAGE:	80

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMASSVLGVR VWPARDYTRV TIESDQPLQN SQQLLQGPDR LVVDLTGLEL DQALRDLVSK IAPNDPQIQS VRVGQYQPHV VRMVFDLKGS VKPQVFTLPP VGTYKYRLVF DLYPAVAPDP LTDLIAQTER KEQALNDAAR AQQMQPPTAL NGPAAAPAPS DNSDAFFQRF AQNTPAAPHT PPAAPSAPAK PAVKPPPVIA RRDDGDDDAD AYKFTAPKSG KGGTVRLLTV AIDPGHGGED PGAIGGAXTY EKHIALDIAK XLRAKIDGAP NMRAMMTRDA XFFVPLNVRV QXXAAXAXLX XSIXXDAFTT XRXXXXXXLX XXXXXRXXXX XXXXLXXGXX XXXXXXXXXX XXXXXXXXXX XXXXRXXX

Validated NT Sequence

ttttgtnnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcgtc gtcggtgctc ggcgtgcgcg tgtggcccgc acgcgattac acgcgcgtga cgatcgaatc cgatcagccg ctgcagaata gccaacagct gctgcagggg cccgaccggc tcgtcgtcga cctgaccggc ctcgagctcg accaggcgct gcgcgacctc gtgtcgaaga tcgcgccgaa cgatccgcag attcaatcgg tgcgcgtcgg ccagtatcag ccgcacgtcg tgcggatggt gttcgacctg aaaggctcgg tgaagccgca ggtgttcacg ctgccgccgg tcggcaccta caagtatcgg ctcgtgttcg acctctatcc ggccgtcgcg cccgatccgc tgaccgacct gatcgcgcag accgaacgca aggagcaggc gctgaacgat gcggcgcgcg cgcagcagat gcagccgccg accgcgctga acggcccggc ggccgcgccc gcgccgagcg acaacagcga cgcgtttttc cagcgcttcg cgcagaacac gccggccgcg ccgcacacgc cgccggccgc gccttcggcg cccgcgaagc cggccgtcaa gccgccgccc gtgatcgcgc gccgcgacga cggcgacgac gacgccgatg cctacaagtt caccgcgccg aagtccggca agggcggcac cgtgcggctg ctgaccgtcg cgatcgatcc gggccacggc ggcgaggatc cgggcgcgat cggcggcgcc ngcacgtacg agaagcacat cgcgctcgac atcgcgaaga anctgcgcgc gaagatcgat ggngcgccna acatgcgcgc gatgatgacg cgcgacgccn acttcttcgt gccgctgaac gtgcgcgtgc aaaanngcgc cgcgtnggcc ganctnntcn tgtcgatccn nnccgatgca ttcacnacnn nncgnnngnn cnnnnnnnnn ncgctnnnnn tcnnnnnngn cnngcgctgn tngcnannnn nnngnnncna nctganngnn ggnnnanntn annnncngan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntnnnnn nnnnnnnnnn ncggnnnntn nnnnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMASSVLGVR VWPARDYTRV TIESDQPLQN SQQLLQGPDR LVVDLTGLEL DQALRDLVSK IAPNDPQIQS VRVGQYQPHV VRMVFDLKGS VKPQVFTLPP VGTYKYRLVF DLYPAVAPDP LTDLIAQTER KEQALNDAAR AQQMQPPTAL NGPAAAPAPS DNSDAFFQRF AQNTPAAPHT PPAAPSAPAK PAVKPPPVIA RRDDGDDDAD AYKFTAPKSG KGGTVRLLTV AIDPGHGGED PGAIGGAGTY EKHIALDIAK KLRAKIDGAP NMRAMMTRDA DFFVPLNVRV QKARRVGADL FVSIHADAFT TPSARGSSVF ALSDHGASSA AARWLANKEN ASDLIGGINI KTQDAAVSRA LFDMSTTAQI RDSLRYGNYV LKEVGSINKL HKGSVEQAGF AVLKAPDIPS ILVETAFISN PDEERRLNDD SYRDQMADAI FRGIKRYFAA NPPLAKNRMA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgtcgtcg gtgctcggcg tgcgcgtgtg gcccgcacgc gattacacgc gcgtgacgat cgaatccgat cagccgctgc agaatagcca acagctgctg caggggcccg accggctcgt cgtcgacctg accggcctcg agctcgacca ggcgctgcgc gacctcgtgt cgaagatcgc gccgaacgat ccgcagattc aatcggtgcg cgtcggccag tatcagccgc acgtcgtgcg gatggtgttc gacctgaaag gctcggtgaa gccgcaggtg ttcacgctgc cgccggtcgg cacctacaag tatcggctcg tgttcgacct ctatccggcc gtcgcgcccg atccgctgac cgacctgatc gcgcagaccg aacgcaagga gcaggcgctg aacgatgcgg cgcgcgcgca gcagatgcag ccgccgaccg cgctgaacgg cccggcggcc gcgcccgcgc cgagcgacaa cagcgacgcg tttttccagc gcttcgcgca gaacacgccg gccgcgccgc acacgccgcc ggccgcgcct tcggcgcccg cgaagccggc cgtcaagccg ccgcccgtga tcgcgcgccg cgacgacggc gacgacgacg ccgatgccta caagttcacc gcgccgaagt ccggcaaggg cggcaccgtg cggctgctga ccgtcgcgat cgatccgggc cacggcggcg aggatccggg cgcgatcggc ggcgccggca cgtacgagaa gcacatcgcg ctcgacatcg cgaagaagct gcgcgcgaag atcgatggcg cgccgaacat gcgcgcgatg atgacgcgcg acgccgactt cttcgtgccg ctgaacgtgc gcgtgcaaaa ggcgcgccgc gtcggcgccg acctgttcgt gtcgatccac gccgatgcat tcacgacgcc gtccgcgcgc ggctcgtcgg tgttcgcgct gtcggatcac ggcgcgtcga gcgcggccgc gcgctggctc gcgaacaagg agaatgcgtc cgatctgatc ggcgggatca acatcaagac gcaggacgcg gcggtgagcc gcgcgctgtt cgacatgtcg accacggcgc agattcgcga ttcgctgcgc tacggcaact acgtgctgaa ggaagtcggc agcatcaaca agctgcacaa gggctcggtc gagcaggcgg gattcgcggt gctgaaggcg cccgacattc cgtcgatcct cgtcgagacc gcgttcatca gcaacccgga cgaggagcgc cggctcaacg acgacagcta tcgcgaccag atggccgacg cgatcttccg cggcatcaag cgctacttcg ccgcgaatcc gccgctcgcg aagaaccgca tggcctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BumuA.11726.a.A2.PS01722

PURIFICATION DATe:	1/11/2012
CONCENTRATION:	84.4mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	82
PERCENT COVERAGE:	80

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMASSVLGVR VWPARDYTRV TIESDQPLQN SQQLLQGPDR LVVDLTGLEL DQALRDLVSK IAPNDPQIQS VRVGQYQPHV VRMVFDLKGS VKPQVFTLPP VGTYKYRLVF DLYPAVAPDP LTDLIAQTER KEQALNDAAR AQQMQPPTAL NGPAAAPAPS DNSDAFFQRF AQNTPAAPHT PPAAPSAPAK PAVKPPPVIA RRDDGDDDAD AYKFTAPKSG KGGTVRLLTV AIDPGHGGED PGAIGGAGTY EKHIALDIAK KLRAKIDGAP NMRAMMTRDA DFFVPLNVRV QKARRVGADL FVSIHADAFT TPSARGSSVF ALSDHGASSA AARWLANKEN ASDLIGGINI KTQDAAVSRA LFDMSTTAQI RDSLRYGNYV LKEVGSINKL HKGSVEQAGF AVLKAPDIPS ILVETAFISN PDEERRLNDD SYRDQMADAI FRGIKRYFAA NPPLAKNRMA

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGTCGTCG GTGCTCGGCG TGCGCGTGTG GCCCGCACGC GATTACACGC GCGTGACGAT CGAATCCGAT CAGCCGCTGC AGAATAGCCA ACAGCTGCTG CAGGGGCCCG ACCGGCTCGT CGTCGACCTG ACCGGCCTCG AGCTCGACCA GGCGCTGCGC GACCTCGTGT CGAAGATCGC GCCGAACGAT CCGCAGATTC AATCGGTGCG CGTCGGCCAG TATCAGCCGC ACGTCGTGCG GATGGTGTTC GACCTGAAAG GCTCGGTGAA GCCGCAGGTG TTCACGCTGC CGCCGGTCGG CACCTACAAG TATCGGCTCG TGTTCGACCT CTATCCGGCC GTCGCGCCCG ATCCGCTGAC CGACCTGATC GCGCAGACCG AACGCAAGGA GCAGGCGCTG AACGATGCGG CGCGCGCGCA GCAGATGCAG CCGCCGACCG CGCTGAACGG CCCGGCGGCC GCGCCCGCGC CGAGCGACAA CAGCGACGCG TTTTTCCAGC GCTTCGCGCA GAACACGCCG GCCGCGCCGC ACACGCCGCC GGCCGCGCCT TCGGCGCCCG CGAAGCCGGC CGTCAAGCCG CCGCCCGTGA TCGCGCGCCG CGACGACGGC GACGACGACG CCGATGCCTA CAAGTTCACC GCGCCGAAGT CCGGCAAGGG CGGCACCGTG CGGCTGCTGA CCGTCGCGAT CGATCCGGGC CACGGCGGCG AGGATCCGGG CGCGATCGGC GGCGCCGGCA CGTACGAGAA GCACATCGCG CTCGACATCG CGAAGAAGCT GCGCGCGAAG ATCGATGGCG CGCCGAACAT GCGCGCGATG ATGACGCGCG ACGCCGACTT CTTCGTGCCG CTGAACGTGC GCGTGCAAAA GGCGCGCCGC GTCGGCGCCG ACCTGTTCGT GTCGATCCAC GCCGATGCAT TCACGACGCC GTCCGCGCGC GGCTCGTCGG TGTTCGCGCT GTCGGATCAC GGCGCGTCGA GCGCGGCCGC GCGCTGGCTC GCGAACAAGG AGAATGCGTC CGATCTGATC GGCGGGATCA ACATCAAGAC GCAGGACGCG GCGGTGAGCC GCGCGCTGTT CGACATGTCG ACCACGGCGC AGATTCGCGA TTCGCTGCGC TACGGCAACT ACGTGCTGAA GGAAGTCGGC AGCATCAACA AGCTGCACAA GGGCTCGGTC GAGCAGGCGG GATTCGCGGT GCTGAAGGCG CCCGACATTC CGTCGATCCT CGTCGAGACC GCGTTCATCA GCAACCCGGA CGAGGAGCGC CGGCTCAACG ACGACAGCTA TCGCGACCAG ATGGCCGACG CGATCTTCCG CGGCATCAAG CGCTACTTCG CCGCGAATCC GCCGCTCGCG AAGAACCGCA TGGCCTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA