BumuA.10647.a

Cell division protein

CENTER ID: BumuA.10647.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.10647.a.B2.GE39708 Mature protein( BumuA.10647.a ) 46 616
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.10647.a.B2.PS37876 Mature protein( BumuA.10647.a ) 46 616

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.3073
RefSeq: YP_001581023.1
UniProt: A0A0H3KKC5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKPPQKRHD VKFSSSPVLS VHLPMWRSKL VVFMLFMAFV ALAARAFWIQ GPGNAFYQKQ GESRYQRTIE LPATRGKILD RNGLVLATSL PVRAIWAIPD AVPDDLDADK INQLGKLLGM TPKELRVKLS EDKGFVYVKR QVPIDVADKV AALDIPGIYQ RNEYKRFYPE GEITAHLIGF TNVEDEGQEG VELGDQKLLS GTSGMRRVIK DRLGHIVEDV AEQIPPHNGT DVDLSIDSKI QYIAYANLKA AVEKFKAKAG AAMVVDVRTG EVLALVNYPT YNPNDRSRLT GEQLRNRILT DVFEPGSIMK PFTVSLALDL HRVTPNTLVE TGNGHFVLDG APITDDAGFG TLTVGGVIQK SSNIGATKIA MTMRPEEMWN MYTSIGLGQA PKVGFPGAAA GRLRPWKSWR RIEQATMSYG YGLSASLFQL ARAYTAIAHD GELMPVTIFK TDPKQPVAGT QVFTPTTARE VRAMLETVVA PGGTSPDAAV PGYRVGGKSG TAYKHEGHGY TRKYRASFVG MAPMPNPRIV VAVSVDEPTA GSHFGGQVSG PVFSAIAGDT LRALNVPPNM PIKQLVVSDD SPAAAPSAGA QKLAAGGKAK HMIVSSTTRN SPGVVR
NT Sequence
ATGAAGAAGC CTCCGCAGAA GCGCCACGAC GTGAAGTTCT CGTCGAGCCC CGTGCTGTCC GTGCATCTGC CGATGTGGCG CTCGAAGCTC GTCGTGTTCA TGCTGTTCAT GGCGTTCGTC GCGCTCGCGG CCCGCGCCTT CTGGATCCAG GGGCCCGGCA ACGCGTTCTA TCAGAAGCAG GGCGAAAGCC GGTATCAGCG CACGATCGAG CTGCCGGCCA CGCGCGGCAA GATCCTCGAC CGCAACGGGC TCGTGCTCGC GACGAGCCTG CCCGTGCGCG CGATCTGGGC GATTCCGGAC GCGGTGCCCG ACGACCTCGA CGCCGACAAG ATCAACCAGC TCGGCAAGCT GCTCGGCATG ACGCCGAAGG AGCTGCGCGT CAAGCTGTCG GAAGACAAGG GCTTCGTCTA CGTGAAGCGC CAGGTGCCGA TCGACGTCGC GGACAAGGTC GCCGCGCTCG ACATTCCCGG CATCTATCAG CGCAACGAAT ACAAGCGCTT CTATCCGGAA GGCGAGATCA CCGCGCACCT GATCGGCTTC ACGAACGTCG AGGACGAAGG GCAGGAGGGC GTCGAGCTCG GCGACCAGAA GCTGCTGTCG GGCACGTCCG GCATGCGCCG CGTGATCAAG GACCGTCTCG GCCACATCGT CGAGGACGTC GCCGAGCAGA TTCCGCCGCA CAACGGCACG GACGTCGATC TGTCGATCGA CAGCAAGATC CAGTACATCG CATACGCGAA CCTGAAGGCC GCCGTCGAGA AGTTCAAGGC GAAGGCCGGC GCGGCGATGG TCGTCGACGT GCGTACCGGC GAAGTGCTCG CGCTCGTCAA CTATCCGACG TACAACCCGA ACGACCGCTC GCGGCTGACC GGCGAGCAAC TGCGCAACCG GATCCTGACC GACGTGTTCG AGCCGGGCTC GATCATGAAG CCGTTTACGG TGTCGCTCGC GCTCGACCTG CACCGCGTGA CGCCGAACAC GCTCGTCGAA ACGGGCAACG GGCATTTCGT GCTCGACGGT GCGCCGATCA CCGACGATGC CGGTTTCGGC ACGCTGACGG TCGGCGGCGT GATCCAGAAG TCGAGCAACA TCGGCGCGAC GAAGATCGCG ATGACGATGC GGCCCGAGGA AATGTGGAAC ATGTATACGA GCATCGGCCT CGGCCAGGCG CCGAAGGTCG GCTTCCCGGG CGCGGCCGCG GGCCGGCTGC GTCCGTGGAA GAGCTGGCGC CGCATCGAGC AGGCGACGAT GTCGTACGGC TACGGGCTGT CCGCCTCGCT GTTCCAGCTC GCGCGCGCCT ACACGGCGAT CGCGCACGAC GGCGAACTGA TGCCCGTGAC CATTTTCAAG ACCGACCCCA AGCAGCCGGT CGCCGGTACG CAGGTGTTTA CGCCGACCAC CGCGCGCGAA GTGCGGGCGA TGCTCGAGAC CGTGGTCGCG CCGGGCGGCA CGTCGCCGGA TGCGGCGGTG CCCGGCTATC GCGTCGGCGG CAAGAGCGGT ACCGCGTACA AGCATGAAGG CCACGGCTAT ACGCGCAAGT ACCGCGCGTC GTTCGTCGGG ATGGCGCCGA TGCCGAATCC GCGGATCGTC GTCGCGGTGT CGGTCGACGA GCCGACAGCC GGCAGCCACT TCGGCGGACA GGTGTCCGGC CCCGTCTTCT CGGCGATCGC CGGCGATACG CTGCGCGCGC TGAACGTGCC GCCGAACATG CCGATCAAGC AGCTCGTCGT GTCGGACGAT TCGCCGGCCG CCGCGCCTTC GGCCGGTGCG CAAAAGCTGG CCGCGGGCGG CAAGGCGAAG CATATGATCG TATCCAGCAC CACACGCAAT TCACCAGGAG TCGTTCGA
Details for BumuA.10647.a.B2.GE39708
HARVESTED ON: 12/3/2015
SEQUENCED ON: 12/7/2015
EXPECTED MW: 62kDa
OBSERVED MW: 62kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA FWIQGPGNAF YQKQGESRYQ RTIELPATRG KILDRNGLVL ATSLPVRAIW AIPDAVPDDL DADKINQLGK LLGMTPKELR VKLSEDKGFV YVKRQVPIDV ADKVAALDIP GIYQRNEYKR FYPEGEITAH LIGFTNVEDE GQEGVELGDQ KLLSGTSGMR RVIKDRLGHI VEDVAEQIPP HNGTXVDLSI DSKIQYIAYA NLKAAVEKFK AKAGAAMVVD VRTGEVLALV XYPTYNPNDR SRLXGEALRN RILTDVFEPG SIMKPFTVSL ALDLHRVTPN TLVETGNGHF VLDGAPITDD AGFGTLTVGG VIQKSSNIGA TKIAMTMRPE EMWNMYTSIG LGQAPKVGFP GAAAGRLRPW KSWRRIEQAT MSYGYGLSAS LFQLARAYTA IAHDGELMPV TIFKTDPKQP VAGTQVFTPT TAREVRAMLE TVVAPGGTSP DAAVPGYRVG GKSGTAYKHE GHGYTRKYRA SFVGMAPMPN PRIVVAVSVD EPTAGSHFGG QVSGPVFSAI AGDTLRALNV PPNMPIKQLV VSDDSPAAAP SAGAQKLAAG GKAKHMIVSS TTXXSPGVVR
Validated NT Sequence
atggctcacc accaccacca ccatatggcc ttctggatcc aggggcccgg caacgcgttc tatcagaagc agggcgaaag ccggtatcag cgcacgatcg agctgccggc cacgcgcggc aagatcctcg accgcaacgg gctcgtgctc gcgacgagcc tgcccgtgcg cgcgatctgg gcgattccgg acgcggtgcc cgacgacctc gacgccgaca agatcaacca gctcggcaag ctgctcggca tgacgccgaa ggagctgcgc gtcaagctgt cggaagacaa gggcttcgtc tacgtgaagc gccaggtgcc gatcgacgtc gcggacaagg tcgccgcgct cgacattccc ggcatctatc agcgcaacga atacaagcgc ttctatccgg aaggcgagat caccgcgcac ctgatcggct tcacgaacgt cgaggacgaa gggcaggagg gcgtcgagct cggcgaccag aagctgctgt cgggcacgtc cggcatgcgc cgcgtgatca aggaccgtct cggccacatc gtcgaggacg tcgccgagca gattccgccg cacaacggca cggangtcga tctgtcgatc gacagcaaga tccagtacat cgcatacgcg aacctgaagg ccgccgtcga gaagttc-aa ggcgaaggcc ggcgcggcga tggtcgtcga cgtgcgtacc ggcgaa-gtg ctcgcgctcg tcnactatcc gacgtacaac -ccgaacgac cgctcgcggc tgancggcga agca-ctgcg caacc-ggat cctgaccgac gtg-ttcgag ccgggctcga tcatgaagcc gttt-acggt gtcgctcgcg ctcgacctgc accgcgtgac gcc-gaa-ca cgctcgtcga aacggg-caa -cggg-catt tcgt-gctcg acgg-tgcgc cgatcacc-g acgatg-cc- ggtttcgg-c acgc-tg-ac ggtcggcgg- cgtgatccag aagtcgagca acatcggcgc gacgaagatc gcgatgacga tgcggcccga ggaaatgtgg aacatgtata cgagcatcgg cctcggccag gcgccgaagg tcggcttccc gggcgcggcc gcgggccggc tgcgtccgtg gaagagctgg cgccgcatcg agcaggcgac gatgtcgtac ggctacgggc tgtccgcctc gctgttccag ctcgcgcgcg cctacacggc gatcgcgcac gacggcgaac tgatgcccgt gaccattttc aagaccgacc ccaagcagcc ggtcgccggt acgcaggtgt ttacgccgac caccgcgcgc gaagtgcggg cgatgctcga gaccgtggtc gcgccgggcg gcacgtcgcc ggatgcggcg gtgcccggct atcgcgtcgg cggcaagagc ggtaccgcgt acaagcatga aggccacggc tatacgcgca agtaccgcgc gtcgttcgtc gggatggcgc cgatgccgaa tccgcggatc gtcgtcgcgg tgtcggtcga cgagccgaca gccggcagcc acttcggcgg acaggtgtcc ggccccgtct tctcggcgat cgccggcgat acgctgcgcg cgctgaacgt gccgccgaac atgccgatca agcagctcgt cgtgtcggac gattcgccgg ccgccgcgcc ttcggccggt gcgcaaaagc tggccgcggg cggcaaggcg aagcatatga tcgtatccag caccacnnnn anttcaccag gagtcgttcg ataa
Expected Protein Sequence
MAHHHHHHAF WIQGPGNAFY QKQGESRYQR TIELPATRGK ILDRNGLVLA TSLPVRAIWA IPDAVPDDLD ADKINQLGKL LGMTPKELRV KLSEDKGFVY VKRQVPIDVA DKVAALDIPG IYQRNEYKRF YPEGEITAHL IGFTNVEDEG QEGVELGDQK LLSGTSGMRR VIKDRLGHIV EDVAEQIPPH NGTDVDLSID SKIQYIAYAN LKAAVEKFKA KAGAAMVVDV RTGEVLALVN YPTYNPNDRS RLTGEQLRNR ILTDVFEPGS IMKPFTVSLA LDLHRVTPNT LVETGNGHFV LDGAPITDDA GFGTLTVGGV IQKSSNIGAT KIAMTMRPEE MWNMYTSIGL GQAPKVGFPG AAAGRLRPWK SWRRIEQATM SYGYGLSASL FQLARAYTAI AHDGELMPVT IFKTDPKQPV AGTQVFTPTT AREVRAMLET VVAPGGTSPD AAVPGYRVGG KSGTAYKHEG HGYTRKYRAS FVGMAPMPNP RIVVAVSVDE PTAGSHFGGQ VSGPVFSAIA GDTLRALNVP PNMPIKQLVV SDDSPAAAPS AGAQKLAAGG KAKHMIVSST TRNSPGVVR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccttct ggatccaggg gcccggcaac gcgttctatc agaagcaggg cgaaagccgg tatcagcgca cgatcgagct gccggccacg cgcggcaaga tcctcgaccg caacgggctc gtgctcgcga cgagcctgcc cgtgcgcgcg atctgggcga ttccggacgc ggtgcccgac gacctcgacg ccgacaagat caaccagctc ggcaagctgc tcggcatgac gccgaaggag ctgcgcgtca agctgtcgga agacaagggc ttcgtctacg tgaagcgcca ggtgccgatc gacgtcgcgg acaaggtcgc cgcgctcgac attcccggca tctatcagcg caacgaatac aagcgcttct atccggaagg cgagatcacc gcgcacctga tcggcttcac gaacgtcgag gacgaagggc aggagggcgt cgagctcggc gaccagaagc tgctgtcggg cacgtccggc atgcgccgcg tgatcaagga ccgtctcggc cacatcgtcg aggacgtcgc cgagcagatt ccgccgcaca acggcacgga cgtcgatctg tcgatcgaca gcaagatcca gtacatcgca tacgcgaacc tgaaggccgc cgtcgagaag ttcaaggcga aggccggcgc ggcgatggtc gtcgacgtgc gtaccggcga agtgctcgcg ctcgtcaact atccgacgta caacccgaac gaccgctcgc ggctgaccgg cgagcaactg cgcaaccgga tcctgaccga cgtgttcgag ccgggctcga tcatgaagcc gtttacggtg tcgctcgcgc tcgacctgca ccgcgtgacg ccgaacacgc tcgtcgaaac gggcaacggg catttcgtgc tcgacggtgc gccgatcacc gacgatgccg gtttcggcac gctgacggtc ggcggcgtga tccagaagtc gagcaacatc ggcgcgacga agatcgcgat gacgatgcgg cccgaggaaa tgtggaacat gtatacgagc atcggcctcg gccaggcgcc gaaggtcggc ttcccgggcg cggccgcggg ccggctgcgt ccgtggaaga gctggcgccg catcgagcag gcgacgatgt cgtacggcta cgggctgtcc gcctcgctgt tccagctcgc gcgcgcctac acggcgatcg cgcacgacgg cgaactgatg cccgtgacca ttttcaagac cgaccccaag cagccggtcg ccggtacgca ggtgtttacg ccgaccaccg cgcgcgaagt gcgggcgatg ctcgagaccg tggtcgcgcc gggcggcacg tcgccggatg cggcggtgcc cggctatcgc gtcggcggca agagcggtac cgcgtacaag catgaaggcc acggctatac gcgcaagtac cgcgcgtcgt tcgtcgggat ggcgccgatg ccgaatccgc ggatcgtcgt cgcggtgtcg gtcgacgagc cgacagccgg cagccacttc ggcggacagg tgtccggccc cgtcttctcg gcgatcgccg gcgatacgct gcgcgcgctg aacgtgccgc cgaacatgcc gatcaagcag ctcgtcgtgt cggacgattc gccggccgcc gcgccttcgg ccggtgcgca aaagctggcc gcgggcggca aggcgaagca tatgatcgta tccagcacca cacgcaattc accaggagtc gttcgatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BumuA.10647.a.B2.PS37876
PURIFICATION DATe: 3/9/2016
CONCENTRATION: 28mg/ml
OBSERVED MW: 66kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA FWIQGPGNAF YQKQGESRYQ RTIELPATRG KILDRNGLVL ATSLPVRAIW AIPDAVPDDL DADKINQLGK LLGMTPKELR VKLSEDKGFV YVKRQVPIDV ADKVAALDIP GIYQRNEYKR FYPEGEITAH LIGFTNVEDE GQEGVELGDQ KLLSGTSGMR RVIKDRLGHI VEDVAEQIPP HNGTXVDLSI DSKIQYIAYA NLKAAVEKFK AKAGAAMVVD VRTGEVLALV XYPTYNPNDR SRLXGEALRN RILTDVFEPG SIMKPFTVSL ALDLHRVTPN TLVETGNGHF VLDGAPITDD AGFGTLTVGG VIQKSSNIGA TKIAMTMRPE EMWNMYTSIG LGQAPKVGFP GAAAGRLRPW KSWRRIEQAT MSYGYGLSAS LFQLARAYTA IAHDGELMPV TIFKTDPKQP VAGTQVFTPT TAREVRAMLE TVVAPGGTSP DAAVPGYRVG GKSGTAYKHE GHGYTRKYRA SFVGMAPMPN PRIVVAVSVD EPTAGSHFGG QVSGPVFSAI AGDTLRALNV PPNMPIKQLV VSDDSPAAAP SAGAQKLAAG GKAKHMIVSS TTXXSPGVVR
Validated NT Sequence
atggctcacc accaccacca ccatatggcc ttctggatcc aggggcccgg caacgcgttc tatcagaagc agggcgaaag ccggtatcag cgcacgatcg agctgccggc cacgcgcggc aagatcctcg accgcaacgg gctcgtgctc gcgacgagcc tgcccgtgcg cgcgatctgg gcgattccgg acgcggtgcc cgacgacctc gacgccgaca agatcaacca gctcggcaag ctgctcggca tgacgccgaa ggagctgcgc gtcaagctgt cggaagacaa gggcttcgtc tacgtgaagc gccaggtgcc gatcgacgtc gcggacaagg tcgccgcgct cgacattccc ggcatctatc agcgcaacga atacaagcgc ttctatccgg aaggcgagat caccgcgcac ctgatcggct tcacgaacgt cgaggacgaa gggcaggagg gcgtcgagct cggcgaccag aagctgctgt cgggcacgtc cggcatgcgc cgcgtgatca aggaccgtct cggccacatc gtcgaggacg tcgccgagca gattccgccg cacaacggca cggangtcga tctgtcgatc gacagcaaga tccagtacat cgcatacgcg aacctgaagg ccgccgtcga gaagttc-aa ggcgaaggcc ggcgcggcga tggtcgtcga cgtgcgtacc ggcgaa-gtg ctcgcgctcg tcnactatcc gacgtacaac -ccgaacgac cgctcgcggc tgancggcga agca-ctgcg caacc-ggat cctgaccgac gtg-ttcgag ccgggctcga tcatgaagcc gttt-acggt gtcgctcgcg ctcgacctgc accgcgtgac gcc-gaa-ca cgctcgtcga aacggg-caa -cggg-catt tcgt-gctcg acgg-tgcgc cgatcacc-g acgatg-cc- ggtttcgg-c acgc-tg-ac ggtcggcgg- cgtgatccag aagtcgagca acatcggcgc gacgaagatc gcgatgacga tgcggcccga ggaaatgtgg aacatgtata cgagcatcgg cctcggccag gcgccgaagg tcggcttccc gggcgcggcc gcgggccggc tgcgtccgtg gaagagctgg cgccgcatcg agcaggcgac gatgtcgtac ggctacgggc tgtccgcctc gctgttccag ctcgcgcgcg cctacacggc gatcgcgcac gacggcgaac tgatgcccgt gaccattttc aagaccgacc ccaagcagcc ggtcgccggt acgcaggtgt ttacgccgac caccgcgcgc gaagtgcggg cgatgctcga gaccgtggtc gcgccgggcg gcacgtcgcc ggatgcggcg gtgcccggct atcgcgtcgg cggcaagagc ggtaccgcgt acaagcatga aggccacggc tatacgcgca agtaccgcgc gtcgttcgtc gggatggcgc cgatgccgaa tccgcggatc gtcgtcgcgg tgtcggtcga cgagccgaca gccggcagcc acttcggcgg acaggtgtcc ggccccgtct tctcggcgat cgccggcgat acgctgcgcg cgctgaacgt gccgccgaac atgccgatca agcagctcgt cgtgtcggac gattcgccgg ccgccgcgcc ttcggccggt gcgcaaaagc tggccgcggg cggcaaggcg aagcatatga tcgtatccag caccacnnnn anttcaccag gagtcgttcg ataa
Expressed Protein Sequence
MAHHHHHHAF WIQGPGNAFY QKQGESRYQR TIELPATRGK ILDRNGLVLA TSLPVRAIWA IPDAVPDDLD ADKINQLGKL LGMTPKELRV KLSEDKGFVY VKRQVPIDVA DKVAALDIPG IYQRNEYKRF YPEGEITAHL IGFTNVEDEG QEGVELGDQK LLSGTSGMRR VIKDRLGHIV EDVAEQIPPH NGTDVDLSID SKIQYIAYAN LKAAVEKFKA KAGAAMVVDV RTGEVLALVN YPTYNPNDRS RLTGEQLRNR ILTDVFEPGS IMKPFTVSLA LDLHRVTPNT LVETGNGHFV LDGAPITDDA GFGTLTVGGV IQKSSNIGAT KIAMTMRPEE MWNMYTSIGL GQAPKVGFPG AAAGRLRPWK SWRRIEQATM SYGYGLSASL FQLARAYTAI AHDGELMPVT IFKTDPKQPV AGTQVFTPTT AREVRAMLET VVAPGGTSPD AAVPGYRVGG KSGTAYKHEG HGYTRKYRAS FVGMAPMPNP RIVVAVSVDE PTAGSHFGGQ VSGPVFSAIA GDTLRALNVP PNMPIKQLVV SDDSPAAAPS AGAQKLAAGG KAKHMIVSST TRNSPGVVR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCTTCT GGATCCAGGG GCCCGGCAAC GCGTTCTATC AGAAGCAGGG CGAAAGCCGG TATCAGCGCA CGATCGAGCT GCCGGCCACG CGCGGCAAGA TCCTCGACCG CAACGGGCTC GTGCTCGCGA CGAGCCTGCC CGTGCGCGCG ATCTGGGCGA TTCCGGACGC GGTGCCCGAC GACCTCGACG CCGACAAGAT CAACCAGCTC GGCAAGCTGC TCGGCATGAC GCCGAAGGAG CTGCGCGTCA AGCTGTCGGA AGACAAGGGC TTCGTCTACG TGAAGCGCCA GGTGCCGATC GACGTCGCGG ACAAGGTCGC CGCGCTCGAC ATTCCCGGCA TCTATCAGCG CAACGAATAC AAGCGCTTCT ATCCGGAAGG CGAGATCACC GCGCACCTGA TCGGCTTCAC GAACGTCGAG GACGAAGGGC AGGAGGGCGT CGAGCTCGGC GACCAGAAGC TGCTGTCGGG CACGTCCGGC ATGCGCCGCG TGATCAAGGA CCGTCTCGGC CACATCGTCG AGGACGTCGC CGAGCAGATT CCGCCGCACA ACGGCACGGA CGTCGATCTG TCGATCGACA GCAAGATCCA GTACATCGCA TACGCGAACC TGAAGGCCGC CGTCGAGAAG TTCAAGGCGA AGGCCGGCGC GGCGATGGTC GTCGACGTGC GTACCGGCGA AGTGCTCGCG CTCGTCAACT ATCCGACGTA CAACCCGAAC GACCGCTCGC GGCTGACCGG CGAGCAACTG CGCAACCGGA TCCTGACCGA CGTGTTCGAG CCGGGCTCGA TCATGAAGCC GTTTACGGTG TCGCTCGCGC TCGACCTGCA CCGCGTGACG CCGAACACGC TCGTCGAAAC GGGCAACGGG CATTTCGTGC TCGACGGTGC GCCGATCACC GACGATGCCG GTTTCGGCAC GCTGACGGTC GGCGGCGTGA TCCAGAAGTC GAGCAACATC GGCGCGACGA AGATCGCGAT GACGATGCGG CCCGAGGAAA TGTGGAACAT GTATACGAGC ATCGGCCTCG GCCAGGCGCC GAAGGTCGGC TTCCCGGGCG CGGCCGCGGG CCGGCTGCGT CCGTGGAAGA GCTGGCGCCG CATCGAGCAG GCGACGATGT CGTACGGCTA CGGGCTGTCC GCCTCGCTGT TCCAGCTCGC GCGCGCCTAC ACGGCGATCG CGCACGACGG CGAACTGATG CCCGTGACCA TTTTCAAGAC CGACCCCAAG CAGCCGGTCG CCGGTACGCA GGTGTTTACG CCGACCACCG CGCGCGAAGT GCGGGCGATG CTCGAGACCG TGGTCGCGCC GGGCGGCACG TCGCCGGATG CGGCGGTGCC CGGCTATCGC GTCGGCGGCA AGAGCGGTAC CGCGTACAAG CATGAAGGCC ACGGCTATAC GCGCAAGTAC CGCGCGTCGT TCGTCGGGAT GGCGCCGATG CCGAATCCGC GGATCGTCGT CGCGGTGTCG GTCGACGAGC CGACAGCCGG CAGCCACTTC GGCGGACAGG TGTCCGGCCC CGTCTTCTCG GCGATCGCCG GCGATACGCT GCGCGCGCTG AACGTGCCGC CGAACATGCC GATCAAGCAG CTCGTCGTGT CGGACGATTC GCCGGCCGCC GCGCCTTCGG CCGGTGCGCA AAAGCTGGCC GCGGGCGGCA AGGCGAAGCA TATGATCGTA TCCAGCACCA CACGCAATTC ACCAGGAGTC GTTCGATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T