SSGCID: Target Page

BumuA.10100.b

Protease Do

CENTER ID:	BumuA.10100.b
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.10100.b.B2.GE39706	Mature protein( BumuA.10100.b )		29	494

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.10100.b.B2.PS37894	Mature protein( BumuA.10100.b )		29	494

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.828
RefSeq:	YP_001578874.1
UniProt:	A0A0H3KH91

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNTRFLARSA VVVAVAAALS AGYVAGTRRA DPQIIAPAHA AAMMPAEAAA KTGIPDFSGL VETYGPAVVN ISAKHVVKQV ARRVPQPQLP IDPSDPFYQF FKRFYGQVPG MGGDAQPDDQ PSASLGSGFI ISPDGYILTN AHVIDGANVV TVKLTDKREY KAKVVGSDKQ SDVAVLKIDA TGLPTVKIGD PAQSKVGQWV VAIGSPYGFD NTVTSGIISA KSRALPDENY TPFIQTDVPV NPGNSGGPLF NLQGEVIGIN SMIYSQTGGF QGLSFAIPIN EAIKVKDELV KTGHVSRGRL GVAVQGLNQT LASSFGLAKP DGALVSSVDP NGPAAKAGLQ PGDVILSVNG TPVVDSTTLP SQIANLKPGS KADLQIWRDK AKQSVTVTLG AMTDAKLASN DGGPVEQGRL GVAVRPLTPQ ERSADNLSHG LIVQQAGGPA ASAGIQPGDV ILAVNGRPVT SPEQLREAVK SAGNSLALLI QRDNAQIFVP VDLS

NT Sequence

ATGAACACCC GATTCCTTGC GCGTAGCGCC GTCGTGGTCG CAGTGGCCGC CGCGCTGTCC GCCGGCTACG TCGCCGGCAC GCGCCGCGCC GATCCGCAGA TCATCGCGCC CGCGCACGCG GCGGCGATGA TGCCGGCCGA AGCGGCCGCG AAGACCGGCA TCCCCGATTT CTCCGGGCTG GTCGAGACTT ACGGTCCGGC CGTCGTCAAC ATCAGCGCAA AGCACGTCGT GAAGCAGGTC GCGCGGCGCG TGCCGCAGCC GCAGTTGCCG ATCGACCCGA GCGATCCGTT CTATCAGTTC TTCAAGCGCT TCTATGGACA GGTGCCGGGC ATGGGCGGCG ACGCGCAGCC GGACGACCAG CCGAGCGCGA GCCTCGGGTC GGGTTTCATC ATCAGCCCGG ACGGATACAT CCTCACGAAT GCGCACGTGA TCGACGGTGC GAACGTCGTC ACGGTAAAGC TGACCGACAA GCGCGAGTAC AAGGCGAAGG TCGTCGGCTC CGACAAGCAG TCCGACGTCG CGGTGCTGAA GATCGACGCC ACCGGCCTGC CGACCGTGAA GATCGGCGAT CCGGCGCAGA GCAAGGTCGG CCAGTGGGTC GTCGCGATCG GCTCGCCGTA CGGCTTCGAC AACACGGTCA CGTCGGGCAT CATCAGCGCG AAGTCGCGCG CGCTGCCCGA CGAGAACTAC ACGCCGTTCA TCCAGACCGA CGTGCCGGTC AACCCCGGCA ACTCGGGCGG CCCGCTGTTC AACCTGCAGG GCGAGGTGAT CGGGATCAAC TCGATGATCT ATTCGCAGAC GGGCGGCTTC CAGGGCCTGT CGTTCGCGAT TCCGATCAAC GAAGCGATCA AGGTGAAGGA CGAGCTCGTG AAGACCGGGC ACGTGAGCCG CGGCCGGCTC GGCGTCGCGG TGCAGGGGCT GAACCAGACG CTCGCGAGCT CGTTCGGGCT CGCGAAGCCG GACGGTGCGC TCGTCAGCTC GGTCGATCCG AACGGCCCGG CCGCGAAGGC CGGCCTGCAG CCGGGCGACG TGATCCTGTC GGTCAACGGC ACGCCGGTCG TCGATTCGAC GACGCTGCCG TCGCAGATCG CGAACCTGAA GCCGGGCTCG AAGGCCGACC TGCAGATCTG GCGCGACAAG GCGAAGCAGT CCGTCACCGT CACGCTCGGC GCGATGACCG ACGCGAAGCT CGCGTCGAAC GACGGCGGTC CGGTCGAGCA GGGGCGCCTC GGCGTCGCGG TGCGGCCGCT GACGCCGCAG GAACGCAGCG CCGACAACCT GTCGCACGGG CTGATCGTCC AGCAGGCCGG CGGCCCGGCC GCGAGCGCCG GCATTCAGCC GGGCGACGTG ATCCTGGCCG TGAACGGCCG CCCCGTCACG AGCCCCGAGC AGCTGCGCGA AGCGGTCAAG AGCGCGGGCA ACAGTCTTGC TCTGCTGATC CAGCGCGATA ATGCACAGAT CTTCGTGCCG GTCGACCTGA GC

Details for BumuA.10100.b.B2.GE39706

HARVESTED ON:	12/3/2015
SEQUENCED ON:	12/7/2015
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMR ADPQIIAPAH AAAMMPAEAA AKTGIPDFSG LVETYGPAVV NISAKHVVKQ VARRVPQPQL PIDPSDPFYQ FFKRFYGQVP GMGGDAQPDD QPSASLGSGF IISPDGYILT NAHVIDGANV VTVKLTDKRE YKAKVVGSDK QSDVAVLKID ATGLPTVKIG DPAQSKVGQW VVAIGSPYGF DNTVTSGIIS AKSRALPDEN YTPFIQTDVP VNPGNSGGPL FNLQGEVIGI NSMIYSQTGG FQGLSFAIPI NEAIKVKDEL VKTGHVSRGR LGVAVQGLNQ TLASSFGLAK PDGALVSSVD PNGPAAKAGL QPGDVILSVN GTPVVDSTTL PSQIANLKPG SKADLQIWRD KAKQSVTVTL GAMTDAKLAS NDGGPVEQGR LGVAVRPLTP QERSADNLSH GLIVQQAGGP AASAGIQPGD VILAVNGRPV TSPEQLREAV KSAGNSLALL IQRD

Validated NT Sequence

ttatcgcgct ggatcagcag agcaagactg ttgcccgcgc tcttgaccgc ttcgcgcagc tgctcggggc tcgtgacggg gcggccgttc acggccagga tcacgtcgcc cggctgaatg ccggcgctcg cggccgggcc gccggcctgc tggacgatca gcccgtgcga caggttgtcg gcgctgcgtt cctgcggcgt cagcggccgc accgcgacgc cgaggcgccc ctgctcgacc ggaccgccgt cgttcgacgc gagcttcgcg tcggtcatcg cgccgagcgt gacggtgacg gactgcttcg ccttgtcgcg ccagatctgc aggtcggcct tcgagcccgg cttcaggttc gcgatctgcg acggcagcgt cgtcgaatcg acgaccggcg tgccgttgac cgacaggatc acgtcgcccg gctgcaggcc ggccttcgcg gccgggccgt tcggatcgac cgagctgacg agcgcaccgt ccggcttcgc gagcccgaac gagctcgcga gcgtctggtt cagcccctgc accgcgacgc cgagccggcc gcggctcacg tgcccggtct tcacgagctc gtccttcacc ttgatcgctt cgttgatcgg aatcgcgaac gacaggccct ggaagccgcc cgtctgcgaa tagatcatcg agttgatccc gatcacctcg ccctgcaggt tgaacagcgg gccgcccgag ttgccggggt tgaccggcac gtcggtctgg atgaacggcg tgtagttctc gtcgggcagc gcgcgcgact tcgcgctgat gatgcccgac gtgaccgtgt tgtcgaagcc gtacggcgag ccgatcgcga cgacccactg gccgaccttg ctctgcgccg gatcgccgat cttcacggtc ggcaggccgg tggcgtcgat cttcagcacc gcgacgtcgg actgcttgtc ggagccgacg accttcgcct tgtactcgcg cttgtcggtc agctttaccg tgacgacgtt cgcaccgtcg atcacgtgcg cattcgtgag gatgtatccg tccgggctga tgatgaaacc cgacccgagg ctcgcgctcg gctggtcgtc cggctgcgcg tcgccgccca tgcccggcac ctgtccatag aagcgcttga agaactgata gaacggatcg ctcgggtcga tcggcaactg cggctgcggc acgcgccgcg cgacctgctt cacgacgtgc tttgcgctga tgttgacgac ggccggaccg taagtctcga ccagcccgga gaaatcgggg atgccggtct tcgcggccgc ttcggccggc atcatcgccg ccgcgtgcgc gggcgcgatg atctgcggat cggcgcgcat atggtggtgg tggtggtgag ccatgg

Expected Protein Sequence

MAHHHHHHRA DPQIIAPAHA AAMMPAEAAA KTGIPDFSGL VETYGPAVVN ISAKHVVKQV ARRVPQPQLP IDPSDPFYQF FKRFYGQVPG MGGDAQPDDQ PSASLGSGFI ISPDGYILTN AHVIDGANVV TVKLTDKREY KAKVVGSDKQ SDVAVLKIDA TGLPTVKIGD PAQSKVGQWV VAIGSPYGFD NTVTSGIISA KSRALPDENY TPFIQTDVPV NPGNSGGPLF NLQGEVIGIN SMIYSQTGGF QGLSFAIPIN EAIKVKDELV KTGHVSRGRL GVAVQGLNQT LASSFGLAKP DGALVSSVDP NGPAAKAGLQ PGDVILSVNG TPVVDSTTLP SQIANLKPGS KADLQIWRDK AKQSVTVTLG AMTDAKLASN DGGPVEQGRL GVAVRPLTPQ ERSADNLSHG LIVQQAGGPA ASAGIQPGDV ILAVNGRPVT SPEQLREAVK SAGNSLALLI QRDNAQIFVP VDLS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgcgccg atccgcagat catcgcgccc gcgcacgcgg cggcgatgat gccggccgaa gcggccgcga agaccggcat ccccgatttc tccgggctgg tcgagactta cggtccggcc gtcgtcaaca tcagcgcaaa gcacgtcgtg aagcaggtcg cgcggcgcgt gccgcagccg cagttgccga tcgacccgag cgatccgttc tatcagttct tcaagcgctt ctatggacag gtgccgggca tgggcggcga cgcgcagccg gacgaccagc cgagcgcgag cctcgggtcg ggtttcatca tcagcccgga cggatacatc ctcacgaatg cgcacgtgat cgacggtgcg aacgtcgtca cggtaaagct gaccgacaag cgcgagtaca aggcgaaggt cgtcggctcc gacaagcagt ccgacgtcgc ggtgctgaag atcgacgcca ccggcctgcc gaccgtgaag atcggcgatc cggcgcagag caaggtcggc cagtgggtcg tcgcgatcgg ctcgccgtac ggcttcgaca acacggtcac gtcgggcatc atcagcgcga agtcgcgcgc gctgcccgac gagaactaca cgccgttcat ccagaccgac gtgccggtca accccggcaa ctcgggcggc ccgctgttca acctgcaggg cgaggtgatc gggatcaact cgatgatcta ttcgcagacg ggcggcttcc agggcctgtc gttcgcgatt ccgatcaacg aagcgatcaa ggtgaaggac gagctcgtga agaccgggca cgtgagccgc ggccggctcg gcgtcgcggt gcaggggctg aaccagacgc tcgcgagctc gttcgggctc gcgaagccgg acggtgcgct cgtcagctcg gtcgatccga acggcccggc cgcgaaggcc ggcctgcagc cgggcgacgt gatcctgtcg gtcaacggca cgccggtcgt cgattcgacg acgctgccgt cgcagatcgc gaacctgaag ccgggctcga aggccgacct gcagatctgg cgcgacaagg cgaagcagtc cgtcaccgtc acgctcggcg cgatgaccga cgcgaagctc gcgtcgaacg acggcggtcc ggtcgagcag gggcgcctcg gcgtcgcggt gcggccgctg acgccgcagg aacgcagcgc cgacaacctg tcgcacgggc tgatcgtcca gcaggccggc ggcccggccg cgagcgccgg cattcagccg ggcgacgtga tcctggccgt gaacggccgc cccgtcacga gccccgagca gctgcgcgaa gcggtcaaga gcgcgggcaa cagtcttgct ctgctgatcc agcgcgataa tgcacagatc ttcgtgccgg tcgacctgag ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BumuA.10100.b.B2.PS37894

PURIFICATION DATe:	3/30/2016
CONCENTRATION:	24.7mg/ml
OBSERVED MW:	48kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCGCGCCG ATCCGCAGAT CATCGCGCCC GCGCACGCGG CGGCGATGAT GCCGGCCGAA GCGGCCGCGA AGACCGGCAT CCCCGATTTC TCCGGGCTGG TCGAGACTTA CGGTCCGGCC GTCGTCAACA TCAGCGCAAA GCACGTCGTG AAGCAGGTCG CGCGGCGCGT GCCGCAGCCG CAGTTGCCGA TCGACCCGAG CGATCCGTTC TATCAGTTCT TCAAGCGCTT CTATGGACAG GTGCCGGGCA TGGGCGGCGA CGCGCAGCCG GACGACCAGC CGAGCGCGAG CCTCGGGTCG GGTTTCATCA TCAGCCCGGA CGGATACATC CTCACGAATG CGCACGTGAT CGACGGTGCG AACGTCGTCA CGGTAAAGCT GACCGACAAG CGCGAGTACA AGGCGAAGGT CGTCGGCTCC GACAAGCAGT CCGACGTCGC GGTGCTGAAG ATCGACGCCA CCGGCCTGCC GACCGTGAAG ATCGGCGATC CGGCGCAGAG CAAGGTCGGC CAGTGGGTCG TCGCGATCGG CTCGCCGTAC GGCTTCGACA ACACGGTCAC GTCGGGCATC ATCAGCGCGA AGTCGCGCGC GCTGCCCGAC GAGAACTACA CGCCGTTCAT CCAGACCGAC GTGCCGGTCA ACCCCGGCAA CTCGGGCGGC CCGCTGTTCA ACCTGCAGGG CGAGGTGATC GGGATCAACT CGATGATCTA TTCGCAGACG GGCGGCTTCC AGGGCCTGTC GTTCGCGATT CCGATCAACG AAGCGATCAA GGTGAAGGAC GAGCTCGTGA AGACCGGGCA CGTGAGCCGC GGCCGGCTCG GCGTCGCGGT GCAGGGGCTG AACCAGACGC TCGCGAGCTC GTTCGGGCTC GCGAAGCCGG ACGGTGCGCT CGTCAGCTCG GTCGATCCGA ACGGCCCGGC CGCGAAGGCC GGCCTGCAGC CGGGCGACGT GATCCTGTCG GTCAACGGCA CGCCGGTCGT CGATTCGACG ACGCTGCCGT CGCAGATCGC GAACCTGAAG CCGGGCTCGA AGGCCGACCT GCAGATCTGG CGCGACAAGG CGAAGCAGTC CGTCACCGTC ACGCTCGGCG CGATGACCGA CGCGAAGCTC GCGTCGAACG ACGGCGGTCC GGTCGAGCAG GGGCGCCTCG GCGTCGCGGT GCGGCCGCTG ACGCCGCAGG AACGCAGCGC CGACAACCTG TCGCACGGGC TGATCGTCCA GCAGGCCGGC GGCCCGGCCG CGAGCGCCGG CATTCAGCCG GGCGACGTGA TCCTGGCCGT GAACGGCCGC CCCGTCACGA GCCCCGAGCA GCTGCGCGAA GCGGTCAAGA GCGCGGGCAA CAGTCTTGCT CTGCTGATCC AGCGCGATAA TGCACAGATC TTCGTGCCGG TCGACCTGAG CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT