SSGCID: Target Page

BumuA.01673.p

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID:	BumuA.01673.p
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.01673.p.A1.GE34726	Full length( BumuA.01673.p )		1	496

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.6200
RefSeq:	YP_001585841.1
UniProt:	A9ASM7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTSQANNGV RCAPALRRGT GALAVAVLLA GCTVGPNFVP PPAPRVARFV PPDDPGAASG SWSGDGRTQA LSTDAALTRD WWTRFGSRAI DSTVAEALTG SPTLDLAEAT LRRSEHALRA GQGIFFPQVD AQASASRQRA WPLRTGQALP ASIFNLFTLS ATVSYTLDLW GGEQRQVEAL AADVDAQRQA LAGAYLMLTA NVVNTMVARA AYRDEAAATR EMIGLLDEQI RLTGAQVTAG AAAYVAVLTL ESQRASLEAA LPALDQKRAQ ADDLLALLAG RYPVDWRTPD LSLDGIELPA SLPDTAPSSL VRRRPDILQA EAQLHVASAQ VGVATAALYP NLTLSASGGF DSVVASKLFA PAGRAWSVGA GLTAPIFHGG TLLNRRRAAQ DAYDEADAAY RQVVLSAFSQ VADTLRALAN DAMALDAQAR AMAAARDALK LTQAGYDAGI AGYVQLLVAD LQYHQARIAW LQTVAQRLQD TVAFYVALGG GWGDAG

NT Sequence

ATGTCTACGT CGCAGGCAAA TAACGGGGTC CGTTGCGCGC CCGCCCTGCG CCGCGGAACC GGCGCGCTGG CCGTCGCCGT GCTGCTCGCC GGATGCACGG TCGGGCCGAA CTTCGTGCCG CCGCCCGCGC CGCGCGTCGC GCGCTTCGTG CCGCCCGACG ATCCGGGCGC GGCGTCCGGC TCCTGGAGCG GCGATGGCCG GACGCAGGCG TTGTCGACGG ACGCGGCGCT CACGCGCGAC TGGTGGACGC GCTTCGGCAG CCGCGCGATC GACTCGACCG TCGCCGAAGC GCTGACCGGC AGCCCGACGC TCGATCTCGC CGAAGCGACG CTGCGGCGCA GCGAGCACGC GCTGCGGGCG GGCCAGGGGA TCTTCTTTCC GCAGGTGGAT GCGCAGGCCA GTGCGTCGCG CCAGCGCGCC TGGCCGCTGC GCACCGGCCA GGCGTTGCCG GCGTCGATCT TCAACCTGTT CACGCTGTCG GCGACGGTCA GCTACACGCT CGACCTGTGG GGCGGCGAAC AGCGCCAGGT CGAAGCGCTC GCGGCCGACG TCGATGCGCA ACGGCAAGCG CTGGCGGGTG CCTACCTGAT GCTGACCGCG AACGTCGTCA ATACGATGGT CGCGCGCGCG GCCTATCGCG ACGAAGCCGC GGCGACGCGC GAAATGATCG GCCTGCTCGA TGAGCAGATT CGTCTGACAG GTGCGCAGGT GACGGCCGGC GCGGCGGCCT ACGTCGCGGT GCTGACGCTG GAATCGCAGC GGGCGTCGCT CGAGGCGGCG CTGCCCGCAC TCGACCAGAA ACGCGCGCAG GCCGATGATC TGCTCGCGCT GCTGGCCGGG CGCTATCCGG TCGACTGGCG GACGCCGGAC CTGTCGCTCG ACGGGATCGA ACTGCCCGCC AGCCTGCCCG ACACGGCACC GTCATCGCTG GTGCGTCGCC GCCCCGACAT CCTGCAGGCT GAAGCCCAGC TGCACGTGGC CAGCGCGCAG GTCGGCGTCG CGACGGCCGC GCTCTACCCG AACCTCACGC TGTCAGCATC GGGCGGGTTC GACAGCGTCG TCGCCAGCAA ACTGTTCGCG CCGGCCGGCC GCGCATGGAG TGTCGGCGCC GGACTCACTG CGCCGATCTT TCATGGCGGC ACGCTGTTGA ACCGGCGGCG CGCCGCGCAG GATGCGTACG ACGAAGCCGA TGCGGCCTAT CGGCAGGTCG TTCTGTCGGC GTTCTCGCAG GTGGCCGACA CGTTGCGCGC GCTTGCCAAC GACGCGATGG CGCTCGATGC GCAGGCGCGC GCGATGGCCG CGGCGCGTGA CGCACTGAAG CTCACGCAGG CCGGTTACGA CGCGGGGATC GCCGGCTACG TCCAGTTGCT GGTCGCCGAC CTGCAATACC ATCAGGCGCG GATTGCCTGG CTGCAGACGG TCGCGCAGCG CCTGCAGGAC ACGGTCGCGT TCTACGTCGC GCTCGGCGGC GGCTGGGGTG ACGCGGGC

Details for BumuA.01673.p.A1.GE34726

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/16/2012
EXPECTED MW:	54kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	51

Validated AA Sequence

Validated NT Sequence

taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgtctacgt cgcaggcaaa taacggggtc cgttgcgcgc ccgccctgcg ccgcggaacc ggcgcgctgg ccgtcgccgt gctgctcgcc ggatgcacgg tcgggccgaa cttcgtgccg ccgcccgcgc cgcgcgtcgc gcgcttcgtg ccgcccgacg atccgggcgc ggcgtccggc tcctggagcg gcgatggccg gacgcaggcg ttgtcgacgg acgcggcgct cacgcgcgac tggtggacgc gcttcggcag ccgcgcgatc gactcgaccg tcgccgaagc gctgaccggc agcccgacgc tcgatctcgc cgaagcgacg ctgcggcgca gcgagcacgc gctgcgggcg ggccagggga tcttctttcc gcaggtggat gcgcaggcca gtgcgtcgcg ccagcgcgcc tggccgctgc gcaccggcca ggcgttgccg gcgtcgatct tcaacctgtt cacgctgtcg gcgacggtca gctacacgct cgacctgtgg ggcggcgaac agcgccaggt cgaagcgctc gcggccgacg tcgatgcgca acggcaagcg ctggcgggtg cctacctgat gctgancgcg aacgtcgtcn atacgatggt cgcgcgcgcg gcctatcgcg acnaanccgc ggcgacgcgc gaaatgatcg gcctgctcga tgagcagatt cgtctgacag gtgcncaggt gacggccggc gcggcggcct ac

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTSQANNG VRCAPALRRG TGALAVAVLL AGCTVGPNFV PPPAPRVARF VPPDDPGAAS GSWSGDGRTQ ALSTDAALTR DWWTRFGSRA IDSTVAEALT GSPTLDLAEA TLRRSEHALR AGQGIFFPQV DAQASASRQR AWPLRTGQAL PASIFNLFTL SATVSYTLDL WGGEQRQVEA LAADVDAQRQ ALAGAYLMLT ANVVNTMVAR AAYRDEAAAT REMIGLLDEQ IRLTGAQVTA GAAAYVAVLT LESQRASLEA ALPALDQKRA QADDLLALLA GRYPVDWRTP DLSLDGIELP ASLPDTAPSS LVRRRPDILQ AEAQLHVASA QVGVATAALY PNLTLSASGG FDSVVASKLF APAGRAWSVG AGLTAPIFHG GTLLNRRRAA QDAYDEADAA YRQVVLSAFS QVADTLRALA NDAMALDAQA RAMAAARDAL KLTQAGYDAG IAGYVQLLVA DLQYHQARIA WLQTVAQRLQ DTVAFYVALG GGWGDAG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctacgtcg caggcaaata acggggtccg ttgcgcgccc gccctgcgcc gcggaaccgg cgcgctggcc gtcgccgtgc tgctcgccgg atgcacggtc gggccgaact tcgtgccgcc gcccgcgccg cgcgtcgcgc gcttcgtgcc gcccgacgat ccgggcgcgg cgtccggctc ctggagcggc gatggccgga cgcaggcgtt gtcgacggac gcggcgctca cgcgcgactg gtggacgcgc ttcggcagcc gcgcgatcga ctcgaccgtc gccgaagcgc tgaccggcag cccgacgctc gatctcgccg aagcgacgct gcggcgcagc gagcacgcgc tgcgggcggg ccaggggatc ttctttccgc aggtggatgc gcaggccagt gcgtcgcgcc agcgcgcctg gccgctgcgc accggccagg cgttgccggc gtcgatcttc aacctgttca cgctgtcggc gacggtcagc tacacgctcg acctgtgggg cggcgaacag cgccaggtcg aagcgctcgc ggccgacgtc gatgcgcaac ggcaagcgct ggcgggtgcc tacctgatgc tgaccgcgaa cgtcgtcaat acgatggtcg cgcgcgcggc ctatcgcgac gaagccgcgg cgacgcgcga aatgatcggc ctgctcgatg agcagattcg tctgacaggt gcgcaggtga cggccggcgc ggcggcctac gtcgcggtgc tgacgctgga atcgcagcgg gcgtcgctcg aggcggcgct gcccgcactc gaccagaaac gcgcgcaggc cgatgatctg ctcgcgctgc tggccgggcg ctatccggtc gactggcgga cgccggacct gtcgctcgac gggatcgaac tgcccgccag cctgcccgac acggcaccgt catcgctggt gcgtcgccgc cccgacatcc tgcaggctga agcccagctg cacgtggcca gcgcgcaggt cggcgtcgcg acggccgcgc tctacccgaa cctcacgctg tcagcatcgg gcgggttcga cagcgtcgtc gccagcaaac tgttcgcgcc ggccggccgc gcatggagtg tcggcgccgg actcactgcg ccgatctttc atggcggcac gctgttgaac cggcggcgcg ccgcgcagga tgcgtacgac gaagccgatg cggcctatcg gcaggtcgtt ctgtcggcgt tctcgcaggt ggccgacacg ttgcgcgcgc ttgccaacga cgcgatggcg ctcgatgcgc aggcgcgcgc gatggccgcg gcgcgtgacg cactgaagct cacgcaggcc ggttacgacg cggggatcgc cggctacgtc cagttgctgg tcgccgacct gcaataccat caggcgcgga ttgcctggct gcagacggtc gcgcagcgcc tgcaggacac ggtcgcgttc tacgtcgcgc tcggcggcgg ctggggtgac gcgggctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa