SSGCID: Target Page

BumuA.01673.m

RND efflux system outer membrane lipoprotein

CENTER ID:	BumuA.01673.m
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.01673.m.A2.GE34727	Mature protein( BumuA.01673.m )		36	576

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.01673.m.A2.PS01472	Mature protein( BumuA.01673.m )		36	576

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.5888
RefSeq:	YP_001585485.1
UniProt:	A0A0H3KZ82

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTCDVARRS RRPRALRRAT AVAVAAAALA AGTLLGACTV GPDFTPPRAD LPAGWHALQR ADAAAPASGA SAPASTPTTD ADPDPRWWRA FGDPLLDRLV ERAARDNLDV QAAVLRIAQA RAQVRAAAAQ GLPDVRASAS YQREQLGLKG FVEDQGLDRQ IDRLGAPGSP LDRLGPGTGA AVQQRARGAL DALESPVNLW QAGFDASWEL DLFGRVRRTV EAADAQAGAA VASRDDALLS LEAEVAQTYL QLRGAQTQRA LADELVDAQR ELRDLTREQA AHGLASDLDV RSADARLAQL RAQLPQFDQQ IVLLKNGLAY LVGGAPGALD DWLDTPRALP GVPPAVPVGL PSTLARRRPD IRRAEADLHA ATADVGVAVA QFYPDVSLTG QVGLRATHVR ELAHWSHLFY AFGPAVSLPI FSGGALVSNL RLTQARQAEA ALAYRQTVLV ALRDVDNALA VCRTDQTRAA ALDDAVRAEQ GALELARDRY RKGLSPFLDV LDAERQWSEG RQQAVQGALQ TTTDLVALYK ALGGGWREGE GGRDGGARAD ARRVDAARAD ASRTDAARDA QAPAQP

NT Sequence

ATGACGACGT GCGACGTCGC CCGCCGCAGT CGACGGCCGC GCGCGCTGCG CCGTGCGACG GCCGTGGCTG TCGCGGCCGC CGCGCTGGCC GCCGGCACGC TGCTGGGCGC GTGCACGGTC GGGCCGGATT TCACACCGCC GCGTGCGGAC CTGCCCGCGG GCTGGCATGC GCTGCAGCGC GCGGACGCGG CCGCGCCCGC GTCGGGCGCG AGCGCGCCGG CTTCCACGCC GACGACGGAC GCCGATCCCG ATCCGCGCTG GTGGCGCGCG TTCGGCGATC CGCTGCTGGA CCGGCTCGTC GAACGCGCGG CGCGCGACAA CCTCGACGTG CAGGCCGCGG TGTTGCGCAT CGCGCAGGCG CGTGCGCAGG TGCGTGCGGC TGCCGCGCAG GGCTTGCCCG ACGTGCGCGC GAGTGCGAGC TATCAGCGCG AGCAGCTCGG CCTGAAGGGG TTCGTCGAAG ATCAGGGGCT GGACCGGCAA ATCGACCGGC TCGGCGCGCC GGGCTCGCCG CTCGACCGGC TCGGGCCAGG CACCGGCGCA GCCGTGCAGC AGCGTGCGCG CGGCGCGCTC GATGCATTGG AATCGCCGGT CAACCTGTGG CAGGCCGGCT TCGACGCGTC GTGGGAGCTC GATCTGTTCG GACGCGTGCG GCGCACGGTA GAGGCCGCCG ATGCGCAGGC GGGTGCGGCC GTCGCGAGCC GCGACGACGC ATTGCTGTCG CTCGAAGCCG AAGTCGCCCA GACCTATCTG CAGCTGCGCG GTGCGCAGAC GCAGCGCGCG CTCGCCGACG AACTCGTGGA CGCGCAGCGC GAACTGCGCG ATCTGACGCG CGAGCAGGCT GCACACGGCC TGGCGAGCGA CCTCGACGTG CGCAGCGCCG ATGCGCGGCT TGCCCAGTTG CGTGCGCAAC TACCGCAGTT CGATCAGCAG ATCGTGCTGC TGAAGAACGG GCTTGCCTAT CTGGTCGGCG GCGCGCCGGG CGCGCTCGAC GACTGGCTCG ACACGCCGCG TGCGCTGCCG GGCGTGCCGC CCGCCGTGCC GGTCGGCCTG CCGTCGACGC TCGCGCGGCG TCGGCCCGAC ATCCGGCGCG CGGAAGCCGA CCTGCACGCG GCGACGGCCG ACGTCGGCGT CGCGGTCGCG CAGTTCTATC CGGACGTGTC GCTGACCGGG CAAGTCGGGC TGCGCGCGAC GCATGTGCGC GAGCTCGCGC ACTGGTCGCA TCTGTTCTAT GCGTTCGGGC CCGCGGTATC GCTGCCGATT TTTTCGGGTG GCGCGCTGGT GTCGAACCTG CGGCTCACGC AGGCGCGTCA GGCGGAGGCG GCGCTCGCGT ATCGGCAGAC CGTGCTCGTC GCGCTGCGCG ACGTCGACAA TGCGCTCGCC GTGTGCCGCA CCGACCAGAC GCGCGCGGCC GCGCTCGACG ATGCCGTGCG CGCGGAGCAG GGCGCGCTGG AACTCGCGCG CGATCGTTAC CGCAAGGGGC TGTCGCCGTT TCTCGACGTG CTCGACGCGG AGCGGCAATG GTCGGAAGGG CGTCAGCAGG CCGTGCAGGG CGCGCTGCAG ACGACGACCG ATCTCGTCGC GCTGTACAAG GCGCTCGGCG GGGGGTGGCG GGAGGGGGAG GGTGGGCGTG ATGGTGGGGC GCGTGCTGAT GCTCGGCGTG TCGATGCTGC GCGTGCCGAT GCTTCGCGAA CCGATGCGGC GCGCGACGCG CAGGCGCCCG CGCAACCG

Details for BumuA.01673.m.A2.GE34727

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	59kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	70
PERCENT COVERAGE:	12

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGACTVDAH DGRWTLAIVP FGGSEVVNRM PSFRRYVRLT DEQAPAQP

Validated NT Sequence

ttgtttactt taagaaggag anataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgggcgcgt gcacggtcga cgcgcacgat gggcgctgga cgctcgcgat cgtgccgttc ggcggcagcg aggtcgtgaa ccggatgccg tcgtttcgcc gctatgtgcg cttgaccgac gagcaggcgc ccgcgcaacc gtaataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag c

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGACTVGPD FTPPRADLPA GWHALQRADA AAPASGASAP ASTPTTDADP DPRWWRAFGD PLLDRLVERA ARDNLDVQAA VLRIAQARAQ VRAAAAQGLP DVRASASYQR EQLGLKGFVE DQGLDRQIDR LGAPGSPLDR LGPGTGAAVQ QRARGALDAL ESPVNLWQAG FDASWELDLF GRVRRTVEAA DAQAGAAVAS RDDALLSLEA EVAQTYLQLR GAQTQRALAD ELVDAQRELR DLTREQAAHG LASDLDVRSA DARLAQLRAQ LPQFDQQIVL LKNGLAYLVG GAPGALDDWL DTPRALPGVP PAVPVGLPST LARRRPDIRR AEADLHAATA DVGVAVAQFY PDVSLTGQVG LRATHVRELA HWSHLFYAFG PAVSLPIFSG GALVSNLRLT QARQAEAALA YRQTVLVALR DVDNALAVCR TDQTRAAALD DAVRAEQGAL ELARDRYRKG LSPFLDVLDA ERQWSEGRQQ AVQGALQTTT DLVALYKALG GGWREGEGGR DGGARADARR VDAARADASR TDAARDAQAP AQP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgcgtgc acggtcgggc cggatttcac accgccgcgt gcggacctgc ccgcgggctg gcatgcgctg cagcgcgcgg acgcggccgc gcccgcgtcg ggcgcgagcg cgccggcttc cacgccgacg acggacgccg atcccgatcc gcgctggtgg cgcgcgttcg gcgatccgct gctggaccgg ctcgtcgaac gcgcggcgcg cgacaacctc gacgtgcagg ccgcggtgtt gcgcatcgcg caggcgcgtg cgcaggtgcg tgcggctgcc gcgcagggct tgcccgacgt gcgcgcgagt gcgagctatc agcgcgagca gctcggcctg aaggggttcg tcgaagatca ggggctggac cggcaaatcg accggctcgg cgcgccgggc tcgccgctcg accggctcgg gccaggcacc ggcgcagccg tgcagcagcg tgcgcgcggc gcgctcgatg cattggaatc gccggtcaac ctgtggcagg ccggcttcga cgcgtcgtgg gagctcgatc tgttcggacg cgtgcggcgc acggtagagg ccgccgatgc gcaggcgggt gcggccgtcg cgagccgcga cgacgcattg ctgtcgctcg aagccgaagt cgcccagacc tatctgcagc tgcgcggtgc gcagacgcag cgcgcgctcg ccgacgaact cgtggacgcg cagcgcgaac tgcgcgatct gacgcgcgag caggctgcac acggcctggc gagcgacctc gacgtgcgca gcgccgatgc gcggcttgcc cagttgcgtg cgcaactacc gcagttcgat cagcagatcg tgctgctgaa gaacgggctt gcctatctgg tcggcggcgc gccgggcgcg ctcgacgact ggctcgacac gccgcgtgcg ctgccgggcg tgccgcccgc cgtgccggtc ggcctgccgt cgacgctcgc gcggcgtcgg cccgacatcc ggcgcgcgga agccgacctg cacgcggcga cggccgacgt cggcgtcgcg gtcgcgcagt tctatccgga cgtgtcgctg accgggcaag tcgggctgcg cgcgacgcat gtgcgcgagc tcgcgcactg gtcgcatctg ttctatgcgt tcgggcccgc ggtatcgctg ccgatttttt cgggtggcgc gctggtgtcg aacctgcggc tcacgcaggc gcgtcaggcg gaggcggcgc tcgcgtatcg gcagaccgtg ctcgtcgcgc tgcgcgacgt cgacaatgcg ctcgccgtgt gccgcaccga ccagacgcgc gcggccgcgc tcgacgatgc cgtgcgcgcg gagcagggcg cgctggaact cgcgcgcgat cgttaccgca aggggctgtc gccgtttctc gacgtgctcg acgcggagcg gcaatggtcg gaagggcgtc agcaggccgt gcagggcgcg ctgcagacga cgaccgatct cgtcgcgctg tacaaggcgc tcggcggggg gtggcgggag ggggagggtg ggcgtgatgg tggggcgcgt gctgatgctc ggcgtgtcga tgctgcgcgt gccgatgctt cgcgaaccga tgcggcgcgc gacgcgcagg cgcccgcgca accgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BumuA.01673.m.A2.PS01472

PURIFICATION DATe:	4/30/2012
CONCENTRATION:	33.84mg/ml
OBSERVED MW:	56kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	70
PERCENT COVERAGE:	12

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGACTVDAH DGRWTLAIVP FGGSEVVNRM PSFRRYVRLT DEQAPAQP

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCGCGTGC ACGGTCGGGC CGGATTTCAC ACCGCCGCGT GCGGACCTGC CCGCGGGCTG GCATGCGCTG CAGCGCGCGG ACGCGGCCGC GCCCGCGTCG GGCGCGAGCG CGCCGGCTTC CACGCCGACG ACGGACGCCG ATCCCGATCC GCGCTGGTGG CGCGCGTTCG GCGATCCGCT GCTGGACCGG CTCGTCGAAC GCGCGGCGCG CGACAACCTC GACGTGCAGG CCGCGGTGTT GCGCATCGCG CAGGCGCGTG CGCAGGTGCG TGCGGCTGCC GCGCAGGGCT TGCCCGACGT GCGCGCGAGT GCGAGCTATC AGCGCGAGCA GCTCGGCCTG AAGGGGTTCG TCGAAGATCA GGGGCTGGAC CGGCAAATCG ACCGGCTCGG CGCGCCGGGC TCGCCGCTCG ACCGGCTCGG GCCAGGCACC GGCGCAGCCG TGCAGCAGCG TGCGCGCGGC GCGCTCGATG CATTGGAATC GCCGGTCAAC CTGTGGCAGG CCGGCTTCGA CGCGTCGTGG GAGCTCGATC TGTTCGGACG CGTGCGGCGC ACGGTAGAGG CCGCCGATGC GCAGGCGGGT GCGGCCGTCG CGAGCCGCGA CGACGCATTG CTGTCGCTCG AAGCCGAAGT CGCCCAGACC TATCTGCAGC TGCGCGGTGC GCAGACGCAG CGCGCGCTCG CCGACGAACT CGTGGACGCG CAGCGCGAAC TGCGCGATCT GACGCGCGAG CAGGCTGCAC ACGGCCTGGC GAGCGACCTC GACGTGCGCA GCGCCGATGC GCGGCTTGCC CAGTTGCGTG CGCAACTACC GCAGTTCGAT CAGCAGATCG TGCTGCTGAA GAACGGGCTT GCCTATCTGG TCGGCGGCGC GCCGGGCGCG CTCGACGACT GGCTCGACAC GCCGCGTGCG CTGCCGGGCG TGCCGCCCGC CGTGCCGGTC GGCCTGCCGT CGACGCTCGC GCGGCGTCGG CCCGACATCC GGCGCGCGGA AGCCGACCTG CACGCGGCGA CGGCCGACGT CGGCGTCGCG GTCGCGCAGT TCTATCCGGA CGTGTCGCTG ACCGGGCAAG TCGGGCTGCG CGCGACGCAT GTGCGCGAGC TCGCGCACTG GTCGCATCTG TTCTATGCGT TCGGGCCCGC GGTATCGCTG CCGATTTTTT CGGGTGGCGC GCTGGTGTCG AACCTGCGGC TCACGCAGGC GCGTCAGGCG GAGGCGGCGC TCGCGTATCG GCAGACCGTG CTCGTCGCGC TGCGCGACGT CGACAATGCG CTCGCCGTGT GCCGCACCGA CCAGACGCGC GCGGCCGCGC TCGACGATGC CGTGCGCGCG GAGCAGGGCG CGCTGGAACT CGCGCGCGAT CGTTACCGCA AGGGGCTGTC GCCGTTTCTC GACGTGCTCG ACGCGGAGCG GCAATGGTCG GAAGGGCGTC AGCAGGCCGT GCAGGGCGCG CTGCAGACGA CGACCGATCT CGTCGCGCTG TACAAGGCGC TCGGCGGGGG GTGGCGGGAG GGGGAGGGTG GGCGTGATGG TGGGGCGCGT GCTGATGCTC GGCGTGTCGA TGCTGCGCGT GCCGATGCTT CGCGAACCGA TGCGGCGCGC GACGCGCAGG CGCCCGCGCA ACCGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA