SSGCID: Target Page

BumuA.01673.g

RND efflux system outer membrane lipoprotein

CENTER ID:	BumuA.01673.g
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.01673.g.A2.GE34724	Mature protein( BumuA.01673.g )		22	502

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.01673.g.A2.PS01455	Mature protein( BumuA.01673.g )		22	502

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.4635
RefSeq:	YP_001584286.1
UniProt:	A0A0H3KLZ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIATGPRRAV AACAIAAAAA LAGCTVGPDY RRPAVDTPAA WHVDPADAYW HPAAPARAPL DATWWTAFGD PQLDALEAEA QRNNQNLKAV AARYDQAKAT LASVASAQYP AVSLNASGQR FRISADRPQT NYATQNVSTV QNDIQVGASV SYELDLFGRV RRTVEAAEAN TDQARDDFAN ARVVLSADLA SSYFALRELD AEIDVVQRSI ALQQKALDYV SARHDLGAVS GLDLLQQRAQ LDATRTQAQL LVRQRAQVET AIATLVGTPA PAFSLPPRVV PINAPALPTG IPSDLLQRRP DVASAERAMA AANAQIGIAR AAYFPRIALS PDIGWDATRF SGLFTVPALL WSIGASASES LFAGGKLQAG VDFAQAGYVA AQANYRQTVL TAFEEVQNAV TGLSVLADAA QQASAAVDDA RRLVSLAQDR YAGGLAPFID VLTAEQQLLT SERQAVQIQG QRAALVVFLA KALGGGWNGA APTSSDMAAA DAAGTRPSAV TR

NT Sequence

GTGATCGCGA CCGGCCCGCG CCGCGCCGTC GCCGCCTGCG CGATCGCGGC GGCAGCCGCC CTCGCCGGCT GCACGGTCGG CCCCGACTAC CGGCGGCCCG CGGTCGACAC GCCGGCCGCG TGGCACGTCG ATCCGGCCGA CGCGTACTGG CATCCGGCCG CGCCGGCGCG CGCGCCGCTC GACGCGACCT GGTGGACCGC ATTCGGCGAT CCGCAGCTCG ACGCGCTCGA AGCCGAGGCG CAGCGCAACA ACCAGAACCT CAAGGCCGTC GCCGCGCGCT ACGACCAGGC GAAGGCGACG CTCGCGTCGG TCGCGTCGGC GCAATATCCG GCCGTCTCGC TGAACGCCAG CGGCCAGCGC TTTCGCATCT CGGCCGACCG GCCGCAGACG AACTACGCGA CGCAGAACGT ATCGACCGTG CAGAACGACA TCCAGGTCGG CGCGAGCGTC AGCTACGAGC TCGACCTGTT CGGCCGCGTG CGGCGCACGG TCGAGGCCGC CGAGGCGAAT ACCGACCAGG CGCGCGACGA CTTCGCGAAC GCGCGCGTCG TGCTGAGCGC CGATCTCGCG TCGAGCTATT TCGCGCTGCG CGAGCTCGAC GCGGAGATCG ACGTCGTGCA GCGCTCGATC GCGCTGCAGC AGAAGGCGCT CGACTATGTG AGCGCGCGCC ACGATCTCGG TGCGGTGTCG GGCCTCGACC TGCTGCAGCA GCGCGCGCAG CTCGACGCGA CGCGCACGCA GGCGCAGTTG CTGGTGCGGC AGCGCGCGCA GGTCGAAACC GCGATCGCGA CGCTCGTCGG CACGCCGGCG CCCGCATTCT CGCTGCCGCC GCGCGTGGTG CCGATCAACG CGCCGGCGCT GCCGACCGGC ATCCCGAGCG ATCTGCTGCA GCGGCGCCCG GACGTCGCGT CGGCCGAGCG CGCGATGGCT GCCGCGAACG CGCAGATCGG CATCGCGCGC GCCGCGTATT TTCCGCGCAT CGCGCTGTCG CCCGACATCG GCTGGGACGC GACGCGCTTC TCCGGTCTGT TCACGGTGCC GGCGCTGCTG TGGTCGATCG GCGCGTCGGC GAGCGAGTCG CTGTTCGCGG GCGGCAAGCT GCAGGCCGGC GTCGATTTCG CGCAGGCCGG CTACGTGGCC GCGCAGGCCA ACTATCGGCA GACGGTGCTC ACCGCGTTCG AGGAGGTGCA AAATGCGGTG ACCGGGCTGT CGGTGCTCGC CGATGCGGCG CAGCAGGCGT CCGCCGCCGT CGACGACGCG CGCCGGCTCG TGTCGCTCGC GCAGGACCGC TACGCGGGCG GGCTCGCGCC CTTCATCGAC GTGCTGACGG CCGAACAGCA GTTGCTGACG AGCGAGCGAC AGGCCGTGCA GATCCAGGGG CAGCGCGCGG CGCTCGTGGT CTTTCTCGCG AAGGCGCTCG GCGGCGGCTG GAACGGCGCG GCCCCGACCT CCTCCGACAT GGCGGCCGCG GACGCCGCCG GCACGCGCCC GTCGGCGGTC ACGCGA

Details for BumuA.01673.g.A2.GE34724

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/16/2012
EXPECTED MW:	53kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAGCTVGPD YRRPAVDTPA AWHVDPADAY WHPAAPARAP LDATWWTAFG DPQLDALEAE AQRNNQNLKA VAARYDQAKA TLASVASAQY PAVSLNASGQ RFRISADRPQ TNYATQNVST VQNDIQVGAS VSYELDLFGR VRRTVEAAEA NTDQARDDFA NARVVLSADL ASSYFALREL DAEIDVVQRS IALQQKALDY VSARHDLGAV SGLDLLQQRA QLDATRTQAQ LLVRQRAXVE TAIATLVGTP APAFSLPPRV VXINAPALPT GIPSDLLQRR PDVASAERAM AAANAQIGIA RAAYFPRIAL SPDIGWDATR FSGLFTVPAL LWSIGASASE SLFAGGKLQA GVDFAQAGYV AAQANYRQTV LTAFEEVQNA VTGLSVLADA AQQASAAVDD ARRLVSLAQD RYAGGLAPFI DVLTAEQQLL TSERQAVQIQ GQRAALVVFL AKALGGGWNG AAPTSSDMAA ADAAGT

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg gctgcacggt cggccccgac taccggcggc ccgcggtcga cacgccggcc gcgtggcacg tcgatccggc cgacgcgtac tggcatccgg ccgcgccggc gcgcgcgccg ctcgacgcga cctggtggac cgcattcggc gatccgcagc tcgacgcgct cgaagccgag gcgcagcgca acaaccagaa cctcaaggcc gtcgccgcgc gctacgacca ggcgaaggcg acgctcgcgt cggtcgcgtc ggcgcaatat ccggccgtct cgctgaacgc cagcggccag cgctttcgca tctcggccga ccggccgcag acgaactacg cgacgcagaa cgtatcgacc gtgcagaacg acatccaggt cggcgcgagc gtcagctacg agctcgacct gttcggccgc gtgcggcgca cggtcgaggc cgccgaggcg aataccgacc aggcgcgcga cgacttcgcg aacgcgcgcg tcgtgctgag cgccgatctc gcgtcgagct atttcgcgct gcgcgagctc gacgcggaga tcgacgtcgt gcagcgctcg atcgcgctgc agcagaaggc gctcgactat gtgagcgcgc gccacgatct cggtgcggtg tcgggcctcg acctgctgca gcagcgcgcg cagctcgacg cgacgcgcac gcaggcgcag ttgctggtgc ggcagcgcgc gcangtcgaa accgcgatcg cgacgctcgt cggcacgccg gcgcccgcat tctcgctgcc gccgcgcgtg gtnncgatca acgcgccggc gctgccgacc ggcatcccga gcgatctgct gcagcggcgc ccggacgtcg cgtcggccga gcgcgcgatg gctgccgcga acgcgcagat cggcatcgcg cgcgccgcgt attttccgcg catcgcgctg tcgcccgaca tcggctggga cgcgacgcgc ttctccggtc tgttcacggt gccggcgctg ctgtggtcga tcggcgcgtc ggcgagcgag tcgctgttcg cgggcggcaa gctgcaggcc ggcgtcgatt tcgcgcaggc cggctacgtg gccgcgcagg ccaactatcg gcagacggtg ctcaccgcgt tcgaggaggt gcaaaatgcg gtgaccgggc tgtcggtgct cgccgatgcg gcgcagcagg cgtccgccgc cgtcgacgac gcgcgccggc tcgtgtcgct cgcgcaggac cgctacgcgg gcgggctcgc gcccttcatc gacgtgctga cggccgaaca gcagttgctg acgagcgagc gacaggccgt gcagatccag gggcagcgcg cggcgctcgt ggtctttctc gcgaaggcgc tcggcggcgg ctggaacggc gcggccccga cctcctccga catggcggcc gcggacgccg ccggcacgcg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAGCTVGPD YRRPAVDTPA AWHVDPADAY WHPAAPARAP LDATWWTAFG DPQLDALEAE AQRNNQNLKA VAARYDQAKA TLASVASAQY PAVSLNASGQ RFRISADRPQ TNYATQNVST VQNDIQVGAS VSYELDLFGR VRRTVEAAEA NTDQARDDFA NARVVLSADL ASSYFALREL DAEIDVVQRS IALQQKALDY VSARHDLGAV SGLDLLQQRA QLDATRTQAQ LLVRQRAQVE TAIATLVGTP APAFSLPPRV VPINAPALPT GIPSDLLQRR PDVASAERAM AAANAQIGIA RAAYFPRIAL SPDIGWDATR FSGLFTVPAL LWSIGASASE SLFAGGKLQA GVDFAQAGYV AAQANYRQTV LTAFEEVQNA VTGLSVLADA AQQASAAVDD ARRLVSLAQD RYAGGLAPFI DVLTAEQQLL TSERQAVQIQ GQRAALVVFL AKALGGGWNG AAPTSSDMAA ADAAGTRPSA VTR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccggctgc acggtcggcc ccgactaccg gcggcccgcg gtcgacacgc cggccgcgtg gcacgtcgat ccggccgacg cgtactggca tccggccgcg ccggcgcgcg cgccgctcga cgcgacctgg tggaccgcat tcggcgatcc gcagctcgac gcgctcgaag ccgaggcgca gcgcaacaac cagaacctca aggccgtcgc cgcgcgctac gaccaggcga aggcgacgct cgcgtcggtc gcgtcggcgc aatatccggc cgtctcgctg aacgccagcg gccagcgctt tcgcatctcg gccgaccggc cgcagacgaa ctacgcgacg cagaacgtat cgaccgtgca gaacgacatc caggtcggcg cgagcgtcag ctacgagctc gacctgttcg gccgcgtgcg gcgcacggtc gaggccgccg aggcgaatac cgaccaggcg cgcgacgact tcgcgaacgc gcgcgtcgtg ctgagcgccg atctcgcgtc gagctatttc gcgctgcgcg agctcgacgc ggagatcgac gtcgtgcagc gctcgatcgc gctgcagcag aaggcgctcg actatgtgag cgcgcgccac gatctcggtg cggtgtcggg cctcgacctg ctgcagcagc gcgcgcagct cgacgcgacg cgcacgcagg cgcagttgct ggtgcggcag cgcgcgcagg tcgaaaccgc gatcgcgacg ctcgtcggca cgccggcgcc cgcattctcg ctgccgccgc gcgtggtgcc gatcaacgcg ccggcgctgc cgaccggcat cccgagcgat ctgctgcagc ggcgcccgga cgtcgcgtcg gccgagcgcg cgatggctgc cgcgaacgcg cagatcggca tcgcgcgcgc cgcgtatttt ccgcgcatcg cgctgtcgcc cgacatcggc tgggacgcga cgcgcttctc cggtctgttc acggtgccgg cgctgctgtg gtcgatcggc gcgtcggcga gcgagtcgct gttcgcgggc ggcaagctgc aggccggcgt cgatttcgcg caggccggct acgtggccgc gcaggccaac tatcggcaga cggtgctcac cgcgttcgag gaggtgcaaa atgcggtgac cgggctgtcg gtgctcgccg atgcggcgca gcaggcgtcc gccgccgtcg acgacgcgcg ccggctcgtg tcgctcgcgc aggaccgcta cgcgggcggg ctcgcgccct tcatcgacgt gctgacggcc gaacagcagt tgctgacgag cgagcgacag gccgtgcaga tccaggggca gcgcgcggcg ctcgtggtct ttctcgcgaa ggcgctcggc ggcggctgga acggcgcggc cccgacctcc tccgacatgg cggccgcgga cgccgccggc acgcgcccgt cggcggtcac gcgataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BumuA.01673.g.A2.PS01455

PURIFICATION DATe:	4/13/2012
CONCENTRATION:	1.9mg/ml
OBSERVED MW:	51kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAGCTVGPD YRRPAVDTPA AWHVDPADAY WHPAAPARAP LDATWWTAFG DPQLDALEAE AQRNNQNLKA VAARYDQAKA TLASVASAQY PAVSLNASGQ RFRISADRPQ TNYATQNVST VQNDIQVGAS VSYELDLFGR VRRTVEAAEA NTDQARDDFA NARVVLSADL ASSYFALREL DAEIDVVQRS IALQQKALDY VSARHDLGAV SGLDLLQQRA QLDATRTQAQ LLVRQRAQVE TAIATLVGTP APAFSLPPRV VPINAPALPT GIPSDLLQRR PDVASAERAM AAANAQIGIA RAAYFPRIAL SPDIGWDATR FSGLFTVPAL LWSIGASASE SLFAGGKLQA GVDFAQAGYV AAQANYRQTV LTAFEEVQNA VTGLSVLADA AQQASAAVDD ARRLVSLAQD RYAGGLAPFI DVLTAEQQLL TSERQAVQIQ GQRAALVVFL AKALGGGWNG AAPTSSDMAA ADAAGTRPSA VTR

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGGCTGC ACGGTCGGCC CCGACTACCG GCGGCCCGCG GTCGACACGC CGGCCGCGTG GCACGTCGAT CCGGCCGACG CGTACTGGCA TCCGGCCGCG CCGGCGCGCG CGCCGCTCGA CGCGACCTGG TGGACCGCAT TCGGCGATCC GCAGCTCGAC GCGCTCGAAG CCGAGGCGCA GCGCAACAAC CAGAACCTCA AGGCCGTCGC CGCGCGCTAC GACCAGGCGA AGGCGACGCT CGCGTCGGTC GCGTCGGCGC AATATCCGGC CGTCTCGCTG AACGCCAGCG GCCAGCGCTT TCGCATCTCG GCCGACCGGC CGCAGACGAA CTACGCGACG CAGAACGTAT CGACCGTGCA GAACGACATC CAGGTCGGCG CGAGCGTCAG CTACGAGCTC GACCTGTTCG GCCGCGTGCG GCGCACGGTC GAGGCCGCCG AGGCGAATAC CGACCAGGCG CGCGACGACT TCGCGAACGC GCGCGTCGTG CTGAGCGCCG ATCTCGCGTC GAGCTATTTC GCGCTGCGCG AGCTCGACGC GGAGATCGAC GTCGTGCAGC GCTCGATCGC GCTGCAGCAG AAGGCGCTCG ACTATGTGAG CGCGCGCCAC GATCTCGGTG CGGTGTCGGG CCTCGACCTG CTGCAGCAGC GCGCGCAGCT CGACGCGACG CGCACGCAGG CGCAGTTGCT GGTGCGGCAG CGCGCGCAGG TCGAAACCGC GATCGCGACG CTCGTCGGCA CGCCGGCGCC CGCATTCTCG CTGCCGCCGC GCGTGGTGCC GATCAACGCG CCGGCGCTGC CGACCGGCAT CCCGAGCGAT CTGCTGCAGC GGCGCCCGGA CGTCGCGTCG GCCGAGCGCG CGATGGCTGC CGCGAACGCG CAGATCGGCA TCGCGCGCGC CGCGTATTTT CCGCGCATCG CGCTGTCGCC CGACATCGGC TGGGACGCGA CGCGCTTCTC CGGTCTGTTC ACGGTGCCGG CGCTGCTGTG GTCGATCGGC GCGTCGGCGA GCGAGTCGCT GTTCGCGGGC GGCAAGCTGC AGGCCGGCGT CGATTTCGCG CAGGCCGGCT ACGTGGCCGC GCAGGCCAAC TATCGGCAGA CGGTGCTCAC CGCGTTCGAG GAGGTGCAAA ATGCGGTGAC CGGGCTGTCG GTGCTCGCCG ATGCGGCGCA GCAGGCGTCC GCCGCCGTCG ACGACGCGCG CCGGCTCGTG TCGCTCGCGC AGGACCGCTA CGCGGGCGGG CTCGCGCCCT TCATCGACGT GCTGACGGCC GAACAGCAGT TGCTGACGAG CGAGCGACAG GCCGTGCAGA TCCAGGGGCA GCGCGCGGCG CTCGTGGTCT TTCTCGCGAA GGCGCTCGGC GGCGGCTGGA ACGGCGCGGC CCCGACCTCC TCCGACATGG CGGCCGCGGA CGCCGCCGGC ACGCGCCCGT CGGCGGTCAC GCGATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA