BumuA.01673.a

RND efflux system outer membrane lipoprotein

CENTER ID: BumuA.01673.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.01673.a.A2.GE31910 Mature protein( BumuA.01673.a ) 29 498
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.01673.a.A2.PW31823 Mature protein( BumuA.01673.a ) 29 498

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.1964
RefSeq: YP_001579935.1
UniProt: A0A0H3KJ81

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSSPLSVRA GSRRTAVAVA VAALALAGCA NYIGIKSDKQ IAPASQFETA QSLPAQGGHW PSLDWASQFG DPQLPKLIDE ALAGNPSIAQ AQARIAKASS YIESSRSNLL PKAEASYSWT RELYSANGLF PPPYGGQWYS ENNVLASASW ELDLWGKNRE RLRTAVSQEK AAEADMQQAR ITLASSVART YNSLAQLYAL RDIAQREIAN RESVGKITDG RVSAGLDTNV ERQTARGNIA TTQASLSDLD GQITTVRYQL AALLGKGPDR GLQIAAPVLN PAGDVALPDN LPADLVSRRP DIVAARWQVE AAMHDVKEAK AEFFPDVNLA AGFGFDAFGW GKFLNFTSRQ AQFGPAIHLP IFDAGALRAQ LKGRYADFDL SVANYNQTLI SALNDVATQV AAIRAIDRQM GDAQRALDAS TRAYDLAVIR YKAGLSPQLQ VLTADSNRLA SEQTVTNLKM RRRDMQLALI KALGGGFDAT GTALAAPETA KQTRQAAN
NT Sequence
ATGAAATCCT CCCCGTTGTC CGTGCGTGCC GGGTCGCGCC GCACCGCCGT TGCCGTTGCG GTCGCCGCAC TGGCACTGGC GGGCTGCGCG AACTACATCG GCATCAAGAG CGACAAGCAG ATCGCCCCCG CGTCGCAGTT CGAAACCGCG CAGAGCCTTC CGGCCCAGGG CGGCCACTGG CCGTCGCTCG ACTGGGCGAG CCAGTTCGGC GATCCGCAGC TGCCGAAGCT GATCGACGAG GCGCTCGCCG GCAATCCGTC GATCGCGCAG GCGCAGGCGC GCATCGCGAA GGCGTCGTCG TATATCGAAT CGTCGCGCTC GAACCTGCTG CCGAAGGCCG AAGCGAGCTA CTCGTGGACG CGCGAGCTCT ATTCGGCGAA CGGCCTCTTC CCGCCGCCGT ACGGCGGCCA GTGGTACAGC GAGAACAACG TGCTCGCCAG CGCATCGTGG GAGCTCGACC TGTGGGGCAA GAACCGCGAG CGCCTGCGCA CGGCCGTGTC GCAGGAAAAG GCCGCGGAAG CCGACATGCA GCAGGCGCGC ATCACGCTCG CCTCGTCGGT CGCGCGCACC TACAACTCGC TCGCGCAGCT GTACGCGCTG CGCGACATCG CGCAGCGCGA GATCGCCAAC CGCGAGTCGG TCGGCAAGAT CACCGACGGC CGCGTGTCGG CCGGCCTCGA CACGAACGTC GAACGCCAGA CCGCACGCGG CAACATCGCG ACGACGCAGG CGTCGCTGTC CGATCTCGAC GGTCAGATCA CGACCGTGCG CTACCAGCTC GCCGCACTGC TCGGCAAAGG GCCGGACCGC GGGCTGCAGA TCGCCGCGCC GGTGCTGAAC CCGGCCGGCG ACGTCGCGCT ACCCGACAAC CTGCCCGCCG ACCTCGTATC GCGCCGCCCC GACATCGTCG CCGCGCGCTG GCAGGTCGAA GCGGCAATGC ACGACGTGAA GGAAGCGAAG GCCGAGTTCT TCCCGGACGT GAACCTCGCG GCCGGTTTCG GTTTCGATGC GTTCGGCTGG GGCAAATTCC TGAACTTCAC GAGCCGCCAG GCGCAGTTCG GCCCGGCGAT CCATCTGCCG ATCTTCGACG CCGGCGCGCT GCGCGCGCAG CTCAAGGGCC GCTACGCGGA CTTCGATCTG TCGGTCGCGA ACTACAACCA GACGCTGATC AGCGCGCTGA ACGACGTGGC GACGCAAGTT GCCGCGATCC GTGCGATCGA TCGCCAGATG GGCGACGCGC AGCGCGCGCT CGACGCGTCG ACGCGCGCAT ACGATCTCGC GGTGATTCGC TACAAGGCCG GCCTGTCGCC GCAGCTGCAG GTGCTGACCG CGGACAGCAA CCGGCTCGCC TCGGAGCAGA CCGTGACGAA TCTGAAGATG CGCCGTCGCG ACATGCAGCT CGCGCTGATC AAGGCGCTCG GCGGCGGCTT CGATGCGACC GGCACCGCGC TCGCGGCGCC CGAGACCGCG AAACAGACCC GACAGGCCGC CAAC
Details for BumuA.01673.a.A2.GE31910
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 47
PERCENT COVERAGE: 95
Validated AA Sequence
MCANYIGIKS DKQIAPASQF ETAQSLPAQG GHWPSLDWAS QFXXPQLPKL IDEALAGNPS IAQAQARIAK ASSYIESSRS NLLPKAEASY SWTRELYSAN GLFPPPYGGQ WYSENNVLAS ASWELDLWGK NRERLRTAVS QEXXRGSRXA AGAHHARLVX XAHLQLARAA VRAARHXXXR DXQPRVGRXD HRRXRVXRPR HERRTPDRTR QHRDDAGVAV RSXRSDHDRA LPARRTARQR AGPRAADRRA GAEPGRRRRA TRQPARRPRI APPRHRRRAL AGRSGNARRE GSEGRVLPGR EPRGRFRFRC VRLGQIPELH EPPGAVRPGD PSADLRRRRA ARAAQGPLRG LRSVGRELQP DADQRAERRG DASCRDPCDR SPDGRRAARA RRVDARIRSR GDSLQGRPVA AAAGADRGQQ PARLGADRDE SEDAPSRHAA RADQGARRRL RCDRHRARGA RDRETDPTGR Q
Validated NT Sequence
gttggcggcc tgtcgggtct gtttcgcggt ctcgggcgcc gcgagcgcgg tgccggtcgc atcgaagccg ccgccgagcg ccttgatcag cgcgagctgc atgtcgcgac ggcgcatctt cagattcgtc acggtctgct ccgaggcgag ccggttgctg tccgcggtca gcacctgcag ctgcggcgac aggccggcct tgtagcgaat caccgcgaga tcgtatgcgc gcgtcgacgc gtcgagcgcg cgctgcgcgt cgcccatctg gcgatcgatc gcacggatcg cggcaacttg cgtcgccacg tcgttcagcg cgctgatcag cgtctggttg tagttcgcga ccgacagatc gaagtccgcg tagcggccct tgagctgcgc gcgcagcgcg ccggcgtcga agatcggcag atggatcgcc gggccgaact gcgcctggcg gctcgtgaag ttcaggaatt tgccccagcc gaacgcatcg aaaccgaaac cggccgcgag gttcacgtcc gggaagaact cggccttcgc ttccttcacg tcgtgcattg ccgcttcgac ctgccagcgc gcggcgacga tgtcggggcg gcgcgatacg aggtcggcgg gcaggttgtc gggtagcgcg acgtcgccgg ccgggttcag caccggcgcg gcgatctgca gcccgcggtc cggccctttg ccgagcagtg cggcgagctg gtagcgcacg gtcgtgatct gancgtcnag atcggacagc gacgcctgcg tcgtcgcgat gttgccgcgt gcggtctggc gttcgacgtt cgtgtcgagg ccggncgaca cgcgnccgtc ggtgatctnn ccgaccgact cgcggttggn gatctcgnnn nnnnngatgt cgcgcagcgc gtacagctgc gcgagcgagt tgtaggtgcg cgcnnnnnac gaggcgagcg tgatgcgcgc ctgctgcang tcggcttccg cggncntttt cctgcgacac ggccgtgcgc aggcgctcgc ggttcttgcc ccacaggtcg agctcccacg atgcgctggc gagcacgttg ttctcgctgt accactggcc gccgtacggc ggcgggaaga ggccgttcgc cgaatagagc tcgcgcgtcc acgagtagct cgcttcggcc ttcggcagca ggttcgagcg cgacgattcg atatacgacg acgccttcgc gatgcgcgcc tgcgcctgcg cgatcgacgg attgccggcg agcgcctcgt cgatcagctt cggcagctgn ggatngcnga actggctcgc ccagtcgagc gacggccagt ggccgccctg ggccggaagg ctctgcgcgg tttcgaactg cgacgcgggg gcgatctgct tgtcgctctt gatgccgatg tagttcgcgc acatcgaacc aggacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCANYIGIK SDKQIAPASQ FETAQSLPAQ GGHWPSLDWA SQFGDPQLPK LIDEALAGNP SIAQAQARIA KASSYIESSR SNLLPKAEAS YSWTRELYSA NGLFPPPYGG QWYSENNVLA SASWELDLWG KNRERLRTAV SQEKAAEADM QQARITLASS VARTYNSLAQ LYALRDIAQR EIANRESVGK ITDGRVSAGL DTNVERQTAR GNIATTQASL SDLDGQITTV RYQLAALLGK GPDRGLQIAA PVLNPAGDVA LPDNLPADLV SRRPDIVAAR WQVEAAMHDV KEAKAEFFPD VNLAAGFGFD AFGWGKFLNF TSRQAQFGPA IHLPIFDAGA LRAQLKGRYA DFDLSVANYN QTLISALNDV ATQVAAIRAI DRQMGDAQRA LDASTRAYDL AVIRYKAGLS PQLQVLTADS NRLASEQTVT NLKMRRRDMQ LALIKALGGG FDATGTALAA PETAKQTRQA AN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgcgcgaac tacatcggca tcaagagcga caagcagatc gcccccgcgt cgcagttcga aaccgcgcag agccttccgg cccagggcgg ccactggccg tcgctcgact gggcgagcca gttcggcgat ccgcagctgc cgaagctgat cgacgaggcg ctcgccggca atccgtcgat cgcgcaggcg caggcgcgca tcgcgaaggc gtcgtcgtat atcgaatcgt cgcgctcgaa cctgctgccg aaggccgaag cgagctactc gtggacgcgc gagctctatt cggcgaacgg cctcttcccg ccgccgtacg gcggccagtg gtacagcgag aacaacgtgc tcgccagcgc atcgtgggag ctcgacctgt ggggcaagaa ccgcgagcgc ctgcgcacgg ccgtgtcgca ggaaaaggcc gcggaagccg acatgcagca ggcgcgcatc acgctcgcct cgtcggtcgc gcgcacctac aactcgctcg cgcagctgta cgcgctgcgc gacatcgcgc agcgcgagat cgccaaccgc gagtcggtcg gcaagatcac cgacggccgc gtgtcggccg gcctcgacac gaacgtcgaa cgccagaccg cacgcggcaa catcgcgacg acgcaggcgt cgctgtccga tctcgacggt cagatcacga ccgtgcgcta ccagctcgcc gcactgctcg gcaaagggcc ggaccgcggg ctgcagatcg ccgcgccggt gctgaacccg gccggcgacg tcgcgctacc cgacaacctg cccgccgacc tcgtatcgcg ccgccccgac atcgtcgccg cgcgctggca ggtcgaagcg gcaatgcacg acgtgaagga agcgaaggcc gagttcttcc cggacgtgaa cctcgcggcc ggtttcggtt tcgatgcgtt cggctggggc aaattcctga acttcacgag ccgccaggcg cagttcggcc cggcgatcca tctgccgatc ttcgacgccg gcgcgctgcg cgcgcagctc aagggccgct acgcggactt cgatctgtcg gtcgcgaact acaaccagac gctgatcagc gcgctgaacg acgtggcgac gcaagttgcc gcgatccgtg cgatcgatcg ccagatgggc gacgcgcagc gcgcgctcga cgcgtcgacg cgcgcatacg atctcgcggt gattcgctac aaggccggcc tgtcgccgca gctgcaggtg ctgaccgcgg acagcaaccg gctcgcctcg gagcagaccg tgacgaatct gaagatgcgc cgtcgcgaca tgcagctcgc gctgatcaag gcgctcggcg gcggcttcga tgcgaccggc accgcgctcg cggcgcccga gaccgcgaaa cagacccgac aggccgccaa ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BumuA.01673.a.A2.PW31823
PURIFICATION DATe: 5/13/2011
CONCENTRATION: 7.79mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 200µl
PERCENT IDENTITY: 47
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MCANYIGIKS DKQIAPASQF ETAQSLPAQG GHWPSLDWAS QFXXPQLPKL IDEALAGNPS IAQAQARIAK ASSYIESSRS NLLPKAEASY SWTRELYSAN GLFPPPYGGQ WYSENNVLAS ASWELDLWGK NRERLRTAVS QEXXRGSRXA AGAHHARLVX XAHLQLARAA VRAARHXXXR DXQPRVGRXD HRRXRVXRPR HERRTPDRTR QHRDDAGVAV RSXRSDHDRA LPARRTARQR AGPRAADRRA GAEPGRRRRA TRQPARRPRI APPRHRRRAL AGRSGNARRE GSEGRVLPGR EPRGRFRFRC VRLGQIPELH EPPGAVRPGD PSADLRRRRA ARAAQGPLRG LRSVGRELQP DADQRAERRG DASCRDPCDR SPDGRRAARA RRVDARIRSR GDSLQGRPVA AAAGADRGQQ PARLGADRDE SEDAPSRHAA RADQGARRRL RCDRHRARGA RDRETDPTGR Q
Validated NT Sequence
gttggcggcc tgtcgggtct gtttcgcggt ctcgggcgcc gcgagcgcgg tgccggtcgc atcgaagccg ccgccgagcg ccttgatcag cgcgagctgc atgtcgcgac ggcgcatctt cagattcgtc acggtctgct ccgaggcgag ccggttgctg tccgcggtca gcacctgcag ctgcggcgac aggccggcct tgtagcgaat caccgcgaga tcgtatgcgc gcgtcgacgc gtcgagcgcg cgctgcgcgt cgcccatctg gcgatcgatc gcacggatcg cggcaacttg cgtcgccacg tcgttcagcg cgctgatcag cgtctggttg tagttcgcga ccgacagatc gaagtccgcg tagcggccct tgagctgcgc gcgcagcgcg ccggcgtcga agatcggcag atggatcgcc gggccgaact gcgcctggcg gctcgtgaag ttcaggaatt tgccccagcc gaacgcatcg aaaccgaaac cggccgcgag gttcacgtcc gggaagaact cggccttcgc ttccttcacg tcgtgcattg ccgcttcgac ctgccagcgc gcggcgacga tgtcggggcg gcgcgatacg aggtcggcgg gcaggttgtc gggtagcgcg acgtcgccgg ccgggttcag caccggcgcg gcgatctgca gcccgcggtc cggccctttg ccgagcagtg cggcgagctg gtagcgcacg gtcgtgatct gancgtcnag atcggacagc gacgcctgcg tcgtcgcgat gttgccgcgt gcggtctggc gttcgacgtt cgtgtcgagg ccggncgaca cgcgnccgtc ggtgatctnn ccgaccgact cgcggttggn gatctcgnnn nnnnngatgt cgcgcagcgc gtacagctgc gcgagcgagt tgtaggtgcg cgcnnnnnac gaggcgagcg tgatgcgcgc ctgctgcang tcggcttccg cggncntttt cctgcgacac ggccgtgcgc aggcgctcgc ggttcttgcc ccacaggtcg agctcccacg atgcgctggc gagcacgttg ttctcgctgt accactggcc gccgtacggc ggcgggaaga ggccgttcgc cgaatagagc tcgcgcgtcc acgagtagct cgcttcggcc ttcggcagca ggttcgagcg cgacgattcg atatacgacg acgccttcgc gatgcgcgcc tgcgcctgcg cgatcgacgg attgccggcg agcgcctcgt cgatcagctt cggcagctgn ggatngcnga actggctcgc ccagtcgagc gacggccagt ggccgccctg ggccggaagg ctctgcgcgg tttcgaactg cgacgcgggg gcgatctgct tgtcgctctt gatgccgatg tagttcgcgc acatcgaacc aggacc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCANYIGIK SDKQIAPASQ FETAQSLPAQ GGHWPSLDWA SQFGDPQLPK LIDEALAGNP SIAQAQARIA KASSYIESSR SNLLPKAEAS YSWTRELYSA NGLFPPPYGG QWYSENNVLA SASWELDLWG KNRERLRTAV SQEKAAEADM QQARITLASS VARTYNSLAQ LYALRDIAQR EIANRESVGK ITDGRVSAGL DTNVERQTAR GNIATTQASL SDLDGQITTV RYQLAALLGK GPDRGLQIAA PVLNPAGDVA LPDNLPADLV SRRPDIVAAR WQVEAAMHDV KEAKAEFFPD VNLAAGFGFD AFGWGKFLNF TSRQAQFGPA IHLPIFDAGA LRAQLKGRYA DFDLSVANYN QTLISALNDV ATQVAAIRAI DRQMGDAQRA LDASTRAYDL AVIRYKAGLS PQLQVLTADS NRLASEQTVT NLKMRRRDMQ LALIKALGGG FDATGTALAA PETAKQTRQA AN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTGCGCGAAC TACATCGGCA TCAAGAGCGA CAAGCAGATC GCCCCCGCGT CGCAGTTCGA AACCGCGCAG AGCCTTCCGG CCCAGGGCGG CCACTGGCCG TCGCTCGACT GGGCGAGCCA GTTCGGCGAT CCGCAGCTGC CGAAGCTGAT CGACGAGGCG CTCGCCGGCA ATCCGTCGAT CGCGCAGGCG CAGGCGCGCA TCGCGAAGGC GTCGTCGTAT ATCGAATCGT CGCGCTCGAA CCTGCTGCCG AAGGCCGAAG CGAGCTACTC GTGGACGCGC GAGCTCTATT CGGCGAACGG CCTCTTCCCG CCGCCGTACG GCGGCCAGTG GTACAGCGAG AACAACGTGC TCGCCAGCGC ATCGTGGGAG CTCGACCTGT GGGGCAAGAA CCGCGAGCGC CTGCGCACGG CCGTGTCGCA GGAAAAGGCC GCGGAAGCCG ACATGCAGCA GGCGCGCATC ACGCTCGCCT CGTCGGTCGC GCGCACCTAC AACTCGCTCG CGCAGCTGTA CGCGCTGCGC GACATCGCGC AGCGCGAGAT CGCCAACCGC GAGTCGGTCG GCAAGATCAC CGACGGCCGC GTGTCGGCCG GCCTCGACAC GAACGTCGAA CGCCAGACCG CACGCGGCAA CATCGCGACG ACGCAGGCGT CGCTGTCCGA TCTCGACGGT CAGATCACGA CCGTGCGCTA CCAGCTCGCC GCACTGCTCG GCAAAGGGCC GGACCGCGGG CTGCAGATCG CCGCGCCGGT GCTGAACCCG GCCGGCGACG TCGCGCTACC CGACAACCTG CCCGCCGACC TCGTATCGCG CCGCCCCGAC ATCGTCGCCG CGCGCTGGCA GGTCGAAGCG GCAATGCACG ACGTGAAGGA AGCGAAGGCC GAGTTCTTCC CGGACGTGAA CCTCGCGGCC GGTTTCGGTT TCGATGCGTT CGGCTGGGGC AAATTCCTGA ACTTCACGAG CCGCCAGGCG CAGTTCGGCC CGGCGATCCA TCTGCCGATC TTCGACGCCG GCGCGCTGCG CGCGCAGCTC AAGGGCCGCT ACGCGGACTT CGATCTGTCG GTCGCGAACT ACAACCAGAC GCTGATCAGC GCGCTGAACG ACGTGGCGAC GCAAGTTGCC GCGATCCGTG CGATCGATCG CCAGATGGGC GACGCGCAGC GCGCGCTCGA CGCGTCGACG CGCGCATACG ATCTCGCGGT GATTCGCTAC AAGGCCGGCC TGTCGCCGCA GCTGCAGGTG CTGACCGCGG ACAGCAACCG GCTCGCCTCG GAGCAGACCG TGACGAATCT GAAGATGCGC CGTCGCGACA TGCAGCTCGC GCTGATCAAG GCGCTCGGCG GCGGCTTCGA TGCGACCGGC ACCGCGCTCG CGGCGCCCGA GACCGCGAAA CAGACCCGAC AGGCCGCCAA CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA