SSGCID: Target Page

BumuA.01666.f

OmpR family two-component system response regulator

CENTER ID:	BumuA.01666.f
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.01666.f.A1.GE31906	Full length( BumuA.01666.f )		1	221

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.6176
RefSeq:	YP_001585818.1
UniProt:	A0A0H3KQB7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRILLVEDDI LLGDGIRAGL RQQGFQVDWV RDGDAAQREL RAHAHAAAIL DLGLPLVDGM DVLQRIRRDG MALPILILTA RDAVPDRIRA LDVGADDYVI KPVDLYELGA RLRALVRRAH GRPTERLQAQ DVELEPASRT ARRAGVAVAL STREFDLLHI LMLNAGRVLS REQIELYLYS WGQEVESNAV EVHVHRLRAK LGSELIRTVR GVGYVLMRDA A

NT Sequence

GTGCGCATCC TGCTCGTTGA AGACGACATC CTGCTCGGCG ACGGGATCCG CGCCGGATTG CGCCAGCAAG GATTCCAGGT CGACTGGGTG CGCGACGGCG ACGCTGCCCA GCGCGAACTG CGCGCGCATG CCCATGCCGC GGCGATCCTC GATCTCGGGT TGCCGCTCGT GGATGGCATG GATGTCCTGC AGCGCATTCG TCGCGACGGC ATGGCGTTGC CGATTCTTAT CCTGACTGCT CGCGATGCCG TGCCCGACCG CATCAGGGCG CTCGACGTGG GCGCCGACGA CTACGTGATC AAACCCGTCG ACCTGTACGA GCTCGGCGCG CGCCTGCGCG CGCTGGTCCG GCGCGCCCAC GGCCGGCCGA CCGAGCGGCT TCAGGCCCAG GACGTCGAGC TGGAACCCGC ATCGCGCACC GCGCGCCGCG CCGGCGTGGC CGTCGCGCTA TCCACGCGCG AGTTCGACCT GCTGCACATC CTGATGCTGA ATGCCGGCCG GGTCCTGTCG CGCGAGCAGA TCGAGCTGTA TCTGTACAGC TGGGGGCAGG AGGTGGAGAG CAATGCCGTC GAGGTGCACG TCCATCGCTT GCGTGCGAAG CTCGGCAGCG AGCTGATCCG GACGGTGCGC GGCGTGGGGT ACGTGCTGAT GCGGGACGCT GCA

Details for BumuA.01666.f.A1.GE31906

HARVESTED ON:	3/22/2011
SEQUENCED ON:	3/25/2011
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVRILLVED DILLGDGIRA GLRQQGFQVD WVRDGDAAQR ELRAHAHAAA ILDLGLPLVD GMDVLQRIRR DGMALPILIL TARDAVPDRI RALDVGADDY VIKPVDLYEL GARLRALVRR AHGRPTERLQ AQDVELEPAS RTARRAGVAV ALSTREFDLL HILMLNAGRV LSREQIELYL YSWGQEVESN AVEVHVHRLR AKLGSELIRT VRGVGYVLMR DAA

Validated NT Sequence

ttattatgca gcgtcncgca tcagcacgta cccccacgcc gcgcaccgtc cggatcagct cgctgccgag cttcgcacgc aagcgatgga cgtgcacctc gacggcattg ctctccacct cctgccccca gctgtacaga tacagctcga tctgctcgcg cgacaggacc cggccggcat tcagcatcag gatgtgcagc aggtcgaact cgcgcgtgga tagcgcgacg gccacgccgg cgcggcgcgc ggtgcgcgat gcgggttcca gctcgacgtc ctgggcctga agccgctcgg tcggccggcc gtgggcgcgc cggaccagcg cgcgcaggcg cgcgccgagc tcgtacaggt cgacgggttt gatcacgtag tcgtcggcgc ccacgtcgag cgccctgatg cggtcgggca cggcatcgcg agcagtcagg ataagaatcg gcaacgccat gccgtcgcga cgaatgcgct gcaggacatc catgccatcc acgagcggca acccgagatc gaggatcgcc gcggcatggg catgcgcgcg cagttcgcgc tgggcagcgt cgccgtcgcg cacccagtcg acctggaatc cttgctggcg caatccggcg cggatcccgt cgccgagcag gatgtcgtct tcaacgagca ggatgcgcac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatgg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcgcatc ctgctcgttg aagacgacat cctgctcggc gacgggatcc gcgccggatt gcgccagcaa ggattccagg tcgactgggt gcgcgacggc gacgctgccc agcgcgaact gcgcgcgcat gcccatgccg cggcgatcct cgatctcggg ttgccgctcg tggatggcat ggatgtcctg cagcgcattc gtcgcgacgg catggcgttg ccgattctta tcctgactgc tcgcgatgcc gtgcccgacc gcatcagggc gctcgacgtg ggcgccgacg actacgtgat caaacccgtc gacctgtacg agctcggcgc gcgcctgcgc gcgctggtcc ggcgcgccca cggccggccg accgagcggc ttcaggccca ggacgtcgag ctggaacccg catcgcgcac cgcgcgccgc gccggcgtgg ccgtcgcgct atccacgcgc gagttcgacc tgctgcacat cctgatgctg aatgccggcc gggtcctgtc gcgcgagcag atcgagctgt atctgtacag ctgggggcag gaggtggaga gcaatgccgt cgaggtgcac gtccatcgct tgcgtgcgaa gctcggcagc gagctgatcc ggacggtgcg cggcgtgggg tacgtgctga tgcgggacgc tgcataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa