BumuA.01666.c

Signal transduction histidine kinase, nitrogen specific, NtrB

CENTER ID: BumuA.01666.c
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.01666.c.A1.GE31903 Full length( BumuA.01666.c ) 1 393
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.01666.c.A1.PS01077 Full length( BumuA.01666.c ) 1 393

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.1301
RefSeq: YP_001579310.1
UniProt: A9AGS2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLWRGNARH ARTMVLKNLI KAKTGQPEQL TDDERLARSG LLAGLEALPT VVLVLDRKTL RIAFANPSAE AMLDISRRQL AQRPWGEIFP NANELASTIT AIGEERFHAT HLDTVLDRPG HELLHVHAIV GFLESAPDFV LVELFENERQ SRTDREERIH DLTAVNKQLI RNLAHEIKNP LGGIRGAAQL LEFELGERER GELREYTQVI IKESDRLQTL VDRLLEPHRH PHIVGDVNIH EVCERVRAVM LAEFPRGLTI VRDYDVSVPD LRGDKEQLIQ ALLNIVRNAA QALRERIAQG DAKIELRTRV ARKVTIAKRL YRLALDLHVI DNGPGIPDEI RDRIFYPLVS GRDDGSGLGL TLAQTFVQQH DGTIEVESRP GRTEFQILLP LDA
NT Sequence
ATGAATCTTT GGCGCGGCAA CGCGCGACAC GCACGCACGA TGGTTCTGAA GAATCTGATC AAGGCGAAGA CGGGGCAGCC CGAGCAATTG ACCGACGACG AGCGGCTCGC CCGTTCCGGC CTGCTCGCCG GGCTCGAGGC GCTGCCGACG GTCGTGCTCG TGCTCGACCG CAAGACGCTC AGGATCGCGT TCGCGAATCC GTCCGCGGAG GCGATGCTCG ACATCTCGCG CCGCCAGCTC GCGCAGCGGC CGTGGGGCGA GATTTTCCCG AACGCGAACG AGCTCGCCTC GACGATCACC GCGATCGGCG AGGAGCGCTT TCACGCGACG CATCTGGACA CCGTGCTCGA CCGGCCCGGC CACGAGCTGC TGCACGTGCA TGCGATCGTC GGCTTTCTCG AGAGCGCGCC CGATTTCGTG CTCGTCGAGC TGTTCGAGAA CGAGCGGCAG TCGCGCACCG ATCGCGAGGA GCGCATTCAC GACCTGACCG CGGTCAACAA GCAGCTGATC CGCAATCTCG CGCACGAGAT CAAGAACCCG CTCGGCGGCA TCCGCGGGGC CGCGCAACTG CTCGAATTCG AGCTCGGCGA GCGCGAACGC GGCGAGCTGC GCGAATACAC GCAGGTGATC ATCAAGGAGT CCGATCGCCT GCAGACGCTC GTCGACCGGC TGCTGGAGCC GCACCGGCAT CCGCACATCG TCGGCGACGT GAACATCCAC GAAGTATGCG AACGCGTGCG CGCCGTGATG CTCGCCGAAT TCCCGCGCGG GCTCACGATC GTGCGCGACT ACGACGTCAG CGTGCCGGAC CTGCGCGGCG ACAAGGAGCA GCTGATCCAG GCGCTGCTGA ACATCGTGCG CAACGCGGCG CAGGCATTGC GCGAACGGAT CGCGCAGGGC GACGCGAAGA TCGAACTGCG CACGCGCGTC GCGCGCAAGG TCACGATCGC GAAGCGGCTG TACCGGCTGG CACTGGACTT GCATGTGATC GACAACGGCC CCGGCATTCC GGACGAGATC CGCGACCGGA TCTTCTATCC GCTCGTCTCC GGCCGCGACG ACGGCAGCGG CCTCGGCCTG ACGCTCGCCC AGACGTTCGT GCAGCAGCAC GACGGGACGA TCGAGGTCGA AAGCCGGCCG GGACGTACCG AATTTCAGAT TCTGTTGCCG CTCGACGCA
Details for BumuA.01666.c.A1.GE31903
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 46kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MNLWRGNARH ARTMVLKNLI KAKTGQPEQL TDDERLARSG LLAGLEALPT VVLVLDRKTL RIAFANPSAE AMLDISRRQL AQRPWGEIFP NANELASTIT AIGEERFHAT HLDTVLDRPG HELLHVHAIV GFLESAPDFV LVELFENERQ SRTDREERIH DLTAVNKQLI RNLAHEIKNP LGGIRGAAQL LEFELGERER GELREYTQVI IKESDRLQTL VDRLLEPHRH PHIVGDVNIH EVCERVRAVM LAEFPRGLTI VRDYDVSVPD LRGDKEQLIQ ALLNIVRNAA QALRERIAQG DAKIELRTRV ARKVTIAKRL YRLALDLHVI DNGPGIPDEI RDRIFYPLVS GRDDGSGLGL TLAQTFVQQH DGTIEVESRP GRTEFQILLP L
Validated NT Sequence
cgagcggcaa cagaatctga aattcggtac gtcccggccg gctttcgacc tcgatcgtcc cgtcgtgctg ctgcacgaac gtctgggcga gcgtcaggcc gaggccgctg ccgtcgtcgc ggccggagac gagcggatag aagatccggt cgcggatctc gtccggaatg ccggggccgt tgtcgatcac atgcaagtcc agtgccagcc ggtacagccg cttcgcgatc gtgaccttgc gcgcgacgcg cgtgcgcagt tcgatcttcg cgtcgccctg cgcgatccgt tcgcgcaatg cctgcgccgc gttgcgcacg atgttcagca gcgcctggat cagctgctcc ttgtcgccgc gcaggtccgg cacgctgacg tcgtagtcgc gcacgatcgt gagcccgcgc gggaattcgg cgagcatcac ggcgcgcacg cgttcgcata cttcgtggat gttcacgtcg ccgacgatgt gcggatgccg gtgcggctcc agcagccggt cgacgagcgt ctgcaggcga tcggactcct tgatgatcac ctgcgtgtat tcgcgcagct cgccgcgttc gcgctcgccg agctcgaatt cgagcagttg cgcggccccg cggatgccgc cgagcgggtt cttgatctcg tgcgcgagat tgcggatcag ctgcttgttg accgcggtca ggtcgtgaat gcgctcctcg cgatcggtgc gcgactgccg ctcgttctcg aacagctcga cgagcacgaa atcgggcgcg ctctcgagaa agccgacgat cgcatgcacg tgcagcagct cgtggccggg ccggtcgagc acggtgtcca gatgcgtcgc gtgaaagcgc tcctcgccga tcgcggtgat cgtcgaggcg agctcgttcg cgttcgggaa aatctcgccc cacggccgct gcgcgagctg gcggcgcgag atgtcgagca tcgcctccgc ggacggattc gcgaacgcga tcctgagcgt cttgcggtcg agcacgagca cgaccgtcgg cagcgcctcg agcccggcga gcaggccgga acgggcgagc cgctcgtcgt cggtcaattg ctcgggctgc cccgtcttcg ccttgatcag attcttcaga accatcgtgc gtgcgtgtcg cgcgttgccg cgccaaagat tcatcgaacc aggaccctgg gtctgagctt ccagggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNLWRGNAR HARTMVLKNL IKAKTGQPEQ LTDDERLARS GLLAGLEALP TVVLVLDRKT LRIAFANPSA EAMLDISRRQ LAQRPWGEIF PNANELASTI TAIGEERFHA THLDTVLDRP GHELLHVHAI VGFLESAPDF VLVELFENER QSRTDREERI HDLTAVNKQL IRNLAHEIKN PLGGIRGAAQ LLEFELGERE RGELREYTQV IIKESDRLQT LVDRLLEPHR HPHIVGDVNI HEVCERVRAV MLAEFPRGLT IVRDYDVSVP DLRGDKEQLI QALLNIVRNA AQALRERIAQ GDAKIELRTR VARKVTIAKR LYRLALDLHV IDNGPGIPDE IRDRIFYPLV SGRDDGSGLG LTLAQTFVQQ HDGTIEVESR PGRTEFQILL PLDA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatctttgg cgcggcaacg cgcgacacgc acgcacgatg gttctgaaga atctgatcaa ggcgaagacg gggcagcccg agcaattgac cgacgacgag cggctcgccc gttccggcct gctcgccggg ctcgaggcgc tgccgacggt cgtgctcgtg ctcgaccgca agacgctcag gatcgcgttc gcgaatccgt ccgcggaggc gatgctcgac atctcgcgcc gccagctcgc gcagcggccg tggggcgaga ttttcccgaa cgcgaacgag ctcgcctcga cgatcaccgc gatcggcgag gagcgctttc acgcgacgca tctggacacc gtgctcgacc ggcccggcca cgagctgctg cacgtgcatg cgatcgtcgg ctttctcgag agcgcgcccg atttcgtgct cgtcgagctg ttcgagaacg agcggcagtc gcgcaccgat cgcgaggagc gcattcacga cctgaccgcg gtcaacaagc agctgatccg caatctcgcg cacgagatca agaacccgct cggcggcatc cgcggggccg cgcaactgct cgaattcgag ctcggcgagc gcgaacgcgg cgagctgcgc gaatacacgc aggtgatcat caaggagtcc gatcgcctgc agacgctcgt cgaccggctg ctggagccgc accggcatcc gcacatcgtc ggcgacgtga acatccacga agtatgcgaa cgcgtgcgcg ccgtgatgct cgccgaattc ccgcgcgggc tcacgatcgt gcgcgactac gacgtcagcg tgccggacct gcgcggcgac aaggagcagc tgatccaggc gctgctgaac atcgtgcgca acgcggcgca ggcattgcgc gaacggatcg cgcagggcga cgcgaagatc gaactgcgca cgcgcgtcgc gcgcaaggtc acgatcgcga agcggctgta ccggctggca ctggacttgc atgtgatcga caacggcccc ggcattccgg acgagatccg cgaccggatc ttctatccgc tcgtctccgg ccgcgacgac ggcagcggcc tcggcctgac gctcgcccag acgttcgtgc agcagcacga cgggacgatc gaggtcgaaa gccggccggg acgtaccgaa tttcagattc tgttgccgct cgacgcataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BumuA.01666.c.A1.PS01077
PURIFICATION DATe: 5/11/2011
CONCENTRATION: 9.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MNLWRGNARH ARTMVLKNLI KAKTGQPEQL TDDERLARSG LLAGLEALPT VVLVLDRKTL RIAFANPSAE AMLDISRRQL AQRPWGEIFP NANELASTIT AIGEERFHAT HLDTVLDRPG HELLHVHAIV GFLESAPDFV LVELFENERQ SRTDREERIH DLTAVNKQLI RNLAHEIKNP LGGIRGAAQL LEFELGERER GELREYTQVI IKESDRLQTL VDRLLEPHRH PHIVGDVNIH EVCERVRAVM LAEFPRGLTI VRDYDVSVPD LRGDKEQLIQ ALLNIVRNAA QALRERIAQG DAKIELRTRV ARKVTIAKRL YRLALDLHVI DNGPGIPDEI RDRIFYPLVS GRDDGSGLGL TLAQTFVQQH DGTIEVESRP GRTEFQILLP L
Validated NT Sequence
cgagcggcaa cagaatctga aattcggtac gtcccggccg gctttcgacc tcgatcgtcc cgtcgtgctg ctgcacgaac gtctgggcga gcgtcaggcc gaggccgctg ccgtcgtcgc ggccggagac gagcggatag aagatccggt cgcggatctc gtccggaatg ccggggccgt tgtcgatcac atgcaagtcc agtgccagcc ggtacagccg cttcgcgatc gtgaccttgc gcgcgacgcg cgtgcgcagt tcgatcttcg cgtcgccctg cgcgatccgt tcgcgcaatg cctgcgccgc gttgcgcacg atgttcagca gcgcctggat cagctgctcc ttgtcgccgc gcaggtccgg cacgctgacg tcgtagtcgc gcacgatcgt gagcccgcgc gggaattcgg cgagcatcac ggcgcgcacg cgttcgcata cttcgtggat gttcacgtcg ccgacgatgt gcggatgccg gtgcggctcc agcagccggt cgacgagcgt ctgcaggcga tcggactcct tgatgatcac ctgcgtgtat tcgcgcagct cgccgcgttc gcgctcgccg agctcgaatt cgagcagttg cgcggccccg cggatgccgc cgagcgggtt cttgatctcg tgcgcgagat tgcggatcag ctgcttgttg accgcggtca ggtcgtgaat gcgctcctcg cgatcggtgc gcgactgccg ctcgttctcg aacagctcga cgagcacgaa atcgggcgcg ctctcgagaa agccgacgat cgcatgcacg tgcagcagct cgtggccggg ccggtcgagc acggtgtcca gatgcgtcgc gtgaaagcgc tcctcgccga tcgcggtgat cgtcgaggcg agctcgttcg cgttcgggaa aatctcgccc cacggccgct gcgcgagctg gcggcgcgag atgtcgagca tcgcctccgc ggacggattc gcgaacgcga tcctgagcgt cttgcggtcg agcacgagca cgaccgtcgg cagcgcctcg agcccggcga gcaggccgga acgggcgagc cgctcgtcgt cggtcaattg ctcgggctgc cccgtcttcg ccttgatcag attcttcaga accatcgtgc gtgcgtgtcg cgcgttgccg cgccaaagat tcatcgaacc aggaccctgg gtctgagctt ccagggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNLWRGNAR HARTMVLKNL IKAKTGQPEQ LTDDERLARS GLLAGLEALP TVVLVLDRKT LRIAFANPSA EAMLDISRRQ LAQRPWGEIF PNANELASTI TAIGEERFHA THLDTVLDRP GHELLHVHAI VGFLESAPDF VLVELFENER QSRTDREERI HDLTAVNKQL IRNLAHEIKN PLGGIRGAAQ LLEFELGERE RGELREYTQV IIKESDRLQT LVDRLLEPHR HPHIVGDVNI HEVCERVRAV MLAEFPRGLT IVRDYDVSVP DLRGDKEQLI QALLNIVRNA AQALRERIAQ GDAKIELRTR VARKVTIAKR LYRLALDLHV IDNGPGIPDE IRDRIFYPLV SGRDDGSGLG LTLAQTFVQQ HDGTIEVESR PGRTEFQILL PLDA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATCTTTGG CGCGGCAACG CGCGACACGC ACGCACGATG GTTCTGAAGA ATCTGATCAA GGCGAAGACG GGGCAGCCCG AGCAATTGAC CGACGACGAG CGGCTCGCCC GTTCCGGCCT GCTCGCCGGG CTCGAGGCGC TGCCGACGGT CGTGCTCGTG CTCGACCGCA AGACGCTCAG GATCGCGTTC GCGAATCCGT CCGCGGAGGC GATGCTCGAC ATCTCGCGCC GCCAGCTCGC GCAGCGGCCG TGGGGCGAGA TTTTCCCGAA CGCGAACGAG CTCGCCTCGA CGATCACCGC GATCGGCGAG GAGCGCTTTC ACGCGACGCA TCTGGACACC GTGCTCGACC GGCCCGGCCA CGAGCTGCTG CACGTGCATG CGATCGTCGG CTTTCTCGAG AGCGCGCCCG ATTTCGTGCT CGTCGAGCTG TTCGAGAACG AGCGGCAGTC GCGCACCGAT CGCGAGGAGC GCATTCACGA CCTGACCGCG GTCAACAAGC AGCTGATCCG CAATCTCGCG CACGAGATCA AGAACCCGCT CGGCGGCATC CGCGGGGCCG CGCAACTGCT CGAATTCGAG CTCGGCGAGC GCGAACGCGG CGAGCTGCGC GAATACACGC AGGTGATCAT CAAGGAGTCC GATCGCCTGC AGACGCTCGT CGACCGGCTG CTGGAGCCGC ACCGGCATCC GCACATCGTC GGCGACGTGA ACATCCACGA AGTATGCGAA CGCGTGCGCG CCGTGATGCT CGCCGAATTC CCGCGCGGGC TCACGATCGT GCGCGACTAC GACGTCAGCG TGCCGGACCT GCGCGGCGAC AAGGAGCAGC TGATCCAGGC GCTGCTGAAC ATCGTGCGCA ACGCGGCGCA GGCATTGCGC GAACGGATCG CGCAGGGCGA CGCGAAGATC GAACTGCGCA CGCGCGTCGC GCGCAAGGTC ACGATCGCGA AGCGGCTGTA CCGGCTGGCA CTGGACTTGC ATGTGATCGA CAACGGCCCC GGCATTCCGG ACGAGATCCG CGACCGGATC TTCTATCCGC TCGTCTCCGG CCGCGACGAC GGCAGCGGCC TCGGCCTGAC GCTCGCCCAG ACGTTCGTGC AGCAGCACGA CGGGACGATC GAGGTCGAAA GCCGGCCGGG ACGTACCGAA TTTCAGATTC TGTTGCCGCT CGACGCATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA