BumuA.00171.a

3-oxoacyl-(Acyl-carrier-protein) synthase III

CENTER ID: BumuA.00171.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00171.a.A1.GE32154 Full length( BumuA.00171.a ) 1 329
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00171.a.A1.PW32072 Full length( BumuA.00171.a ) 1 329

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.2405
RefSeq: YP_001580365.1
UniProt: A0A0H3KDH9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQSTLYSRV LGTGSYLPPD RVTNQQLADR LAKEGIETSD EWIVARTGIH ARHFAAPDVT TSDLALEASR RAIEAADIDP QSIDLIIVAT STPDFVFPST ACLLQNKLGI RNGGAAFDVQ AVCSGFAYAV ATADSFIRSG QHRTALVVGA ETFSRILDFK DRTTCVLFGD GAGAVILSAS EEPGVLGSAL HADGSYSHIL CTPGNVNRGV IDGSAFLHMD GQAVFKLAVN VLEKVAIEAL AKANLAPEQV DWLIPHQANI RIMTSTCRKL GLPQERMVVT VGEHGNTSAA SIPLAFDAAV RDGRIQRGQH VLIEGVGGGF TWGASVFRY
NT Sequence
ATGGCCCAAT CGACTCTCTA TTCCCGCGTG CTCGGCACGG GCAGCTATCT GCCGCCCGAC CGTGTCACGA ACCAGCAGCT TGCCGATCGT CTCGCGAAGG AAGGTATCGA GACGAGCGAT GAATGGATCG TGGCACGCAC GGGCATCCAC GCGCGCCATT TCGCCGCGCC GGACGTGACG ACGAGCGATC TCGCGCTCGA GGCGTCGCGC CGCGCGATCG AGGCGGCCGA CATCGATCCG CAGTCGATCG ACCTGATCAT CGTCGCGACT TCGACCCCCG ATTTCGTGTT TCCGAGCACC GCCTGCCTGC TGCAGAACAA GCTCGGCATC CGCAACGGCG GAGCGGCGTT CGACGTTCAG GCCGTATGCT CGGGGTTCGC GTACGCGGTG GCGACGGCCG ACAGCTTCAT CCGCAGCGGC CAGCACCGCA CGGCGCTCGT CGTGGGCGCG GAGACGTTCT CGCGCATCCT CGATTTCAAG GATCGCACCA CCTGTGTGCT GTTCGGCGAC GGCGCAGGCG CGGTGATCCT GTCCGCCTCC GAAGAGCCGG GCGTGCTCGG CAGCGCGCTG CATGCGGACG GCAGCTATTC GCACATCCTC TGCACGCCGG GCAACGTGAA CCGCGGCGTG ATCGACGGCA GCGCGTTCCT GCACATGGAC GGGCAGGCCG TGTTCAAGCT CGCGGTCAAT GTGCTCGAGA AGGTCGCGAT CGAAGCGCTC GCGAAGGCGA ATCTCGCGCC CGAGCAGGTC GACTGGCTGA TTCCGCATCA GGCCAACATC CGGATCATGA CGAGCACGTG CCGCAAGCTC GGGCTGCCGC AGGAACGCAT GGTCGTGACG GTCGGCGAGC ACGGCAACAC GTCCGCCGCG TCGATTCCGC TCGCCTTCGA TGCCGCGGTG CGCGACGGCC GCATCCAGCG CGGTCAGCAC GTGCTGATCG AAGGCGTCGG CGGCGGCTTC ACGTGGGGCG CGTCGGTCTT CCGCTAC
Details for BumuA.00171.a.A1.GE32154
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMAQSTLY SRVLGTGSYL PPDRVTNQQL ADRLAKEGIE TSDEWIVART GIHARHFAAP DVTTSDLALE ASRRAIEAAD IDPQSIDLII VATSTPDFVF PSTACLLQNK LGIRNGGAAF DVQAVCSGFA YAVATADSFI RSGQHRTALV VGAETFSRIL DFKDRTTCVL FGDGAGAVIL SASEEPGVLG SALHADGSYS HILCTPGNVN RGVIDGSAFL HMDGQAVFKL AVNVLEKVAI EALAKANLAP EQVDWLIPHQ ANIRIMTSTC RKLGLPQERM VVTVGEHGNT SAASIPLAFD AAVRDGRIQR GQHVLIEGVG GGFTWGASVF RY
Validated NT Sequence
ttattagtag cggaagaccg acgcgcccca cgtgaagccg ccgccgacgc cttcgatcag cacgtgctga ccgcgctgga tgcggccgtc gcgcaccgcg gcatcgaagg cgagcggaat cgacgcggcg gacgtgttgc cgtgctcgcc gaccgtcacg accatgcgtt cctgcggcag cccgagcttg cggcacgtgc tcgtcatgat ccggatgttg gcctgatgcg gaatcagcca gtcgacctgc tcgggcgcga gattcgcctt cgcgagcgct tcgatcgcga ccttctcgag cacattgacc gcgagcttga acacggcctg cccgtccatg tgcaggaacg cgctgccgtc gatcacgccg cggttcacgt tgcccggcgt gcagaggatg tgcgaatagc tgccgtccgc atgcagcgcg ctgccgagca cgcccggctc ttcggaggcg gacaggatca ccgcgcctgc gccgtcgccg aacagcacac aggtggtgcg atccttgaaa tcgaggatgc gcgagaacgt ctccgcgccc acgacgagcg ccgtgcggtg ctggccgctg cggatgaagc tgtcggccgt cgccaccgcg tacgcgaacc ccgagcatac ggcctgaacg tcgaacgccg ctccgccgtt gcggatgccg agcttgttct gcagcaggca ggcggtgctc ggaaacacga aatcgggggt cgaagtcgcg acgatgatca ggtcgatcga ctgcggatcg atgtcggccg cctcgatcgc gcggcgcgac gcctcgagcg cgagatcgct cgtcgtcacg tccggcgcgg cgaaatggcg cgcgtggatg cccgtgcgtg ccacgatcca ttcatcgctc gtctcgatac cttccttcgc gagacgatcg gcaagctgct ggttcgtgac acggtcgggc ggcagatagc tgcccgtgcc gagcacgcgg gaatagagag tcgattgggc catcgaacca ggaccctggg tctgagcttc cagggtaccc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQSTLYSR VLGTGSYLPP DRVTNQQLAD RLAKEGIETS DEWIVARTGI HARHFAAPDV TTSDLALEAS RRAIEAADID PQSIDLIIVA TSTPDFVFPS TACLLQNKLG IRNGGAAFDV QAVCSGFAYA VATADSFIRS GQHRTALVVG AETFSRILDF KDRTTCVLFG DGAGAVILSA SEEPGVLGSA LHADGSYSHI LCTPGNVNRG VIDGSAFLHM DGQAVFKLAV NVLEKVAIEA LAKANLAPEQ VDWLIPHQAN IRIMTSTCRK LGLPQERMVV TVGEHGNTSA ASIPLAFDAA VRDGRIQRGQ HVLIEGVGGG FTWGASVFRY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcccaatcg actctctatt cccgcgtgct cggcacgggc agctatctgc cgcccgaccg tgtcacgaac cagcagcttg ccgatcgtct cgcgaaggaa ggtatcgaga cgagcgatga atggatcgtg gcacgcacgg gcatccacgc gcgccatttc gccgcgccgg acgtgacgac gagcgatctc gcgctcgagg cgtcgcgccg cgcgatcgag gcggccgaca tcgatccgca gtcgatcgac ctgatcatcg tcgcgacttc gacccccgat ttcgtgtttc cgagcaccgc ctgcctgctg cagaacaagc tcggcatccg caacggcgga gcggcgttcg acgttcaggc cgtatgctcg gggttcgcgt acgcggtggc gacggccgac agcttcatcc gcagcggcca gcaccgcacg gcgctcgtcg tgggcgcgga gacgttctcg cgcatcctcg atttcaagga tcgcaccacc tgtgtgctgt tcggcgacgg cgcaggcgcg gtgatcctgt ccgcctccga agagccgggc gtgctcggca gcgcgctgca tgcggacggc agctattcgc acatcctctg cacgccgggc aacgtgaacc gcggcgtgat cgacggcagc gcgttcctgc acatggacgg gcaggccgtg ttcaagctcg cggtcaatgt gctcgagaag gtcgcgatcg aagcgctcgc gaaggcgaat ctcgcgcccg agcaggtcga ctggctgatt ccgcatcagg ccaacatccg gatcatgacg agcacgtgcc gcaagctcgg gctgccgcag gaacgcatgg tcgtgacggt cggcgagcac ggcaacacgt ccgccgcgtc gattccgctc gccttcgatg ccgcggtgcg cgacggccgc atccagcgcg gtcagcacgt gctgatcgaa ggcgtcggcg gcggcttcac gtggggcgcg tcggtcttcc gctactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BumuA.00171.a.A1.PW32072
PURIFICATION DATe: 6/17/2011
CONCENTRATION: 21.35mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 21
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMAQSTLY SRVLGTGSYL PPDRVTNQQL ADRLAKEGIE TSDEWIVART GIHARHFAAP DVTTSDLALE ASRRAIEAAD IDPQSIDLII VATSTPDFVF PSTACLLQNK LGIRNGGAAF DVQAVCSGFA YAVATADSFI RSGQHRTALV VGAETFSRIL DFKDRTTCVL FGDGAGAVIL SASEEPGVLG SALHADGSYS HILCTPGNVN RGVIDGSAFL HMDGQAVFKL AVNVLEKVAI EALAKANLAP EQVDWLIPHQ ANIRIMTSTC RKLGLPQERM VVTVGEHGNT SAASIPLAFD AAVRDGRIQR GQHVLIEGVG GGFTWGASVF RY
Validated NT Sequence
ttattagtag cggaagaccg acgcgcccca cgtgaagccg ccgccgacgc cttcgatcag cacgtgctga ccgcgctgga tgcggccgtc gcgcaccgcg gcatcgaagg cgagcggaat cgacgcggcg gacgtgttgc cgtgctcgcc gaccgtcacg accatgcgtt cctgcggcag cccgagcttg cggcacgtgc tcgtcatgat ccggatgttg gcctgatgcg gaatcagcca gtcgacctgc tcgggcgcga gattcgcctt cgcgagcgct tcgatcgcga ccttctcgag cacattgacc gcgagcttga acacggcctg cccgtccatg tgcaggaacg cgctgccgtc gatcacgccg cggttcacgt tgcccggcgt gcagaggatg tgcgaatagc tgccgtccgc atgcagcgcg ctgccgagca cgcccggctc ttcggaggcg gacaggatca ccgcgcctgc gccgtcgccg aacagcacac aggtggtgcg atccttgaaa tcgaggatgc gcgagaacgt ctccgcgccc acgacgagcg ccgtgcggtg ctggccgctg cggatgaagc tgtcggccgt cgccaccgcg tacgcgaacc ccgagcatac ggcctgaacg tcgaacgccg ctccgccgtt gcggatgccg agcttgttct gcagcaggca ggcggtgctc ggaaacacga aatcgggggt cgaagtcgcg acgatgatca ggtcgatcga ctgcggatcg atgtcggccg cctcgatcgc gcggcgcgac gcctcgagcg cgagatcgct cgtcgtcacg tccggcgcgg cgaaatggcg cgcgtggatg cccgtgcgtg ccacgatcca ttcatcgctc gtctcgatac cttccttcgc gagacgatcg gcaagctgct ggttcgtgac acggtcgggc ggcagatagc tgcccgtgcc gagcacgcgg gaatagagag tcgattgggc catcgaacca ggaccctggg tctgagcttc cagggtaccc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQSTLYSR VLGTGSYLPP DRVTNQQLAD RLAKEGIETS DEWIVARTGI HARHFAAPDV TTSDLALEAS RRAIEAADID PQSIDLIIVA TSTPDFVFPS TACLLQNKLG IRNGGAAFDV QAVCSGFAYA VATADSFIRS GQHRTALVVG AETFSRILDF KDRTTCVLFG DGAGAVILSA SEEPGVLGSA LHADGSYSHI LCTPGNVNRG VIDGSAFLHM DGQAVFKLAV NVLEKVAIEA LAKANLAPEQ VDWLIPHQAN IRIMTSTCRK LGLPQERMVV TVGEHGNTSA ASIPLAFDAA VRDGRIQRGQ HVLIEGVGGG FTWGASVFRY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCCAATCG ACTCTCTATT CCCGCGTGCT CGGCACGGGC AGCTATCTGC CGCCCGACCG TGTCACGAAC CAGCAGCTTG CCGATCGTCT CGCGAAGGAA GGTATCGAGA CGAGCGATGA ATGGATCGTG GCACGCACGG GCATCCACGC GCGCCATTTC GCCGCGCCGG ACGTGACGAC GAGCGATCTC GCGCTCGAGG CGTCGCGCCG CGCGATCGAG GCGGCCGACA TCGATCCGCA GTCGATCGAC CTGATCATCG TCGCGACTTC GACCCCCGAT TTCGTGTTTC CGAGCACCGC CTGCCTGCTG CAGAACAAGC TCGGCATCCG CAACGGCGGA GCGGCGTTCG ACGTTCAGGC CGTATGCTCG GGGTTCGCGT ACGCGGTGGC GACGGCCGAC AGCTTCATCC GCAGCGGCCA GCACCGCACG GCGCTCGTCG TGGGCGCGGA GACGTTCTCG CGCATCCTCG ATTTCAAGGA TCGCACCACC TGTGTGCTGT TCGGCGACGG CGCAGGCGCG GTGATCCTGT CCGCCTCCGA AGAGCCGGGC GTGCTCGGCA GCGCGCTGCA TGCGGACGGC AGCTATTCGC ACATCCTCTG CACGCCGGGC AACGTGAACC GCGGCGTGAT CGACGGCAGC GCGTTCCTGC ACATGGACGG GCAGGCCGTG TTCAAGCTCG CGGTCAATGT GCTCGAGAAG GTCGCGATCG AAGCGCTCGC GAAGGCGAAT CTCGCGCCCG AGCAGGTCGA CTGGCTGATT CCGCATCAGG CCAACATCCG GATCATGACG AGCACGTGCC GCAAGCTCGG GCTGCCGCAG GAACGCATGG TCGTGACGGT CGGCGAGCAC GGCAACACGT CCGCCGCGTC GATTCCGCTC GCCTTCGATG CCGCGGTGCG CGACGGCCGC ATCCAGCGCG GTCAGCACGT GCTGATCGAA GGCGTCGGCG GCGGCTTCAC GTGGGGCGCG TCGGTCTTCC GCTACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA