BumuA.00166.a

UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase

CENTER ID: BumuA.00166.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00166.a.A1.GE33103 Full length( BumuA.00166.a ) 1 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00166.a.A1.PS01418 Full length( BumuA.00166.a ) 1 305

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.3060
RefSeq: YP_001581010.1
UniProt: A9AI89

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKQRTIKSI VKTVGIGLHS GRKVELTLRP AAPGTGIVFS RVDLPTPVDI PASAMSIGDT RLASVLQKDG ARVSTIEHLM SACAGLGIDN LYVDVTAEEI PIMDGSAASF VFLIQSAGIE EQNAPKRFIK VKKPVEVRDG DKFARLDPFF GFKLKFTIDF RHPAVDKTGQ ALEVDFANTS YVREIARART FGFAHEVEMM RELGLARGGS MDNAIVLDEY RILNNDGLRY DDEFVKHKML DAIGDLYVVG HPLLASYTAY KSGHGLNNAL LRELLAHEDA YEIVTFDDPQ AAPSGFAFDT QTAFA
NT Sequence
ATGCTGAAGC AGCGCACCAT CAAATCGATC GTGAAGACCG TCGGTATCGG TCTCCACTCG GGCCGCAAGG TCGAACTGAC GCTCCGTCCC GCCGCGCCGG GCACGGGGAT CGTGTTTTCG CGCGTCGACT TGCCCACGCC CGTCGATATT CCGGCCAGCG CGATGTCGAT CGGCGACACG CGGCTCGCAT CGGTGCTGCA GAAGGATGGC GCGCGCGTGT CGACGATCGA GCACCTGATG TCCGCGTGCG CGGGGCTCGG CATCGACAAC CTGTATGTCG ACGTGACGGC CGAGGAAATC CCGATCATGG ACGGCAGCGC GGCATCGTTC GTGTTCCTGA TCCAGTCGGC CGGCATCGAA GAGCAGAATG CGCCGAAGCG CTTCATCAAG GTCAAGAAAC CGGTCGAAGT GCGCGACGGC GACAAGTTCG CGCGTCTCGA TCCGTTCTTC GGCTTCAAGC TGAAATTCAC GATCGACTTC CGTCATCCGG CGGTCGACAA GACGGGCCAG GCGCTCGAGG TCGACTTCGC GAACACGTCG TACGTGCGCG AGATCGCGCG TGCACGCACG TTCGGCTTCG CGCATGAAGT CGAGATGATG CGCGAACTGG GCCTGGCGCG CGGCGGCAGC ATGGACAACG CGATCGTGCT CGACGAGTAC CGGATCCTGA ACAACGACGG CCTGCGCTAC GACGACGAAT TCGTGAAGCA CAAGATGCTC GACGCGATCG GCGATCTGTA CGTGGTCGGC CATCCGCTGC TCGCGTCGTA CACCGCGTAC AAGTCGGGGC ACGGGCTGAA CAACGCGCTG CTGCGCGAAC TGCTCGCGCA CGAGGACGCC TACGAGATCG TCACGTTCGA CGATCCGCAA GCTGCGCCGA GCGGCTTCGC GTTCGATACG CAGACGGCCT TCGCG
Details for BumuA.00166.a.A1.GE33103
HARVESTED ON: 8/19/2011
SEQUENCED ON: 8/31/2011
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMLKQRTI KSIVKTVGIG LHSGRKVELT LRPAAPGTGI VFSRVDLPTP VDIPASAMSI GDTRLASVLQ KDGARVSTIE HLMSACAGLG IDNLYVDVTA EEIPIMDGSA ASFVFLIQSA GIEEQNAPKR FIKVKKPVEV RDGDKFARLD PFFGFKLKFT IDFRHPAVDK TGQALEVDFA NTSYVREIAR ARTFGFAHEV EMMRELGLAR GGSMDNAIVL DEYRILNNDG LRYDDEFVKH KMLDAIGDLY VVGHPLLASY TAYKSGHGLN NALLRELLAH EDAYEIVTFD DPQAAPSGFA FDTQTAFA
Validated NT Sequence
ttattacgcg aaggccgtct gcgtatcgaa cgcgaagccg ctcggcgcag cttgcggatc gtcgaacgtg acgatctcgt aggcgtcctc gtgcgcgagc agttcgcgca gcagcgcgtt gttcagcccg tgccccgact tgtacgcggt gtacgacgcg agcagcggat ggccgaccac gtacagatcg ccgatcgcgt cgagcatctt gtgcttcacg aattcgtcgt cgtagcgcag gccgtcgttg ttcaggatcc ggtactcgtc gagcacgatc gcgttgtcca tgctgccgcc gcgcgccagg cccagttcgc gcatcatctc gacttcatgc gcgaagccga acgtgcgtgc acgcgcgatc tcgcgcacgt acgacgtgtt cgcgaagtcg acctcgagcg cctggcccgt cttgtcgacc gccggatgac ggaagtcgat cgtgaatttc agcttgaagc cgaagaacgg atcgagacgc gcgaacttgt cgccgtcgcg cacttcgacc ggtttcttga ccttgatgaa gcgcttcggc gcattctgct cttcgatgcc ggccgactgg atcaggaaca cgaacgatgc cgcgctgccg tccatgatcg ggatttcctc ggccgtcacg tcgacataca ggttgtcgat gccgagcccc gcgcacgcgg acatcaggtg ctcgatcgtc gacacgcgcg cgccatcctt ctgcagcacc gatgcgagcc gcgtgtcgcc gatcgacatc gcgctggccg gaatatcgac gggcgtgggc aagtcgacgc gcgaaaacac gatccccgtg cccggcgcgg cgggacggag cgtcagttcg accttgcggc ccgagtggag accgataccg acggtcttca cgatcgattt gatggtgcgc tgcttcagca tcgaaccagg accctgggtc tgagcttcca gggtacccat atga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKQRTIKS IVKTVGIGLH SGRKVELTLR PAAPGTGIVF SRVDLPTPVD IPASAMSIGD TRLASVLQKD GARVSTIEHL MSACAGLGID NLYVDVTAEE IPIMDGSAAS FVFLIQSAGI EEQNAPKRFI KVKKPVEVRD GDKFARLDPF FGFKLKFTID FRHPAVDKTG QALEVDFANT SYVREIARAR TFGFAHEVEM MRELGLARGG SMDNAIVLDE YRILNNDGLR YDDEFVKHKM LDAIGDLYVV GHPLLASYTA YKSGHGLNNA LLRELLAHED AYEIVTFDDP QAAPSGFAFD TQTAFA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgaagcag cgcaccatca aatcgatcgt gaagaccgtc ggtatcggtc tccactcggg ccgcaaggtc gaactgacgc tccgtcccgc cgcgccgggc acggggatcg tgttttcgcg cgtcgacttg cccacgcccg tcgatattcc ggccagcgcg atgtcgatcg gcgacacgcg gctcgcatcg gtgctgcaga aggatggcgc gcgcgtgtcg acgatcgagc acctgatgtc cgcgtgcgcg gggctcggca tcgacaacct gtatgtcgac gtgacggccg aggaaatccc gatcatggac ggcagcgcgg catcgttcgt gttcctgatc cagtcggccg gcatcgaaga gcagaatgcg ccgaagcgct tcatcaaggt caagaaaccg gtcgaagtgc gcgacggcga caagttcgcg cgtctcgatc cgttcttcgg cttcaagctg aaattcacga tcgacttccg tcatccggcg gtcgacaaga cgggccaggc gctcgaggtc gacttcgcga acacgtcgta cgtgcgcgag atcgcgcgtg cacgcacgtt cggcttcgcg catgaagtcg agatgatgcg cgaactgggc ctggcgcgcg gcggcagcat ggacaacgcg atcgtgctcg acgagtaccg gatcctgaac aacgacggcc tgcgctacga cgacgaattc gtgaagcaca agatgctcga cgcgatcggc gatctgtacg tggtcggcca tccgctgctc gcgtcgtaca ccgcgtacaa gtcggggcac gggctgaaca acgcgctgct gcgcgaactg ctcgcgcacg aggacgccta cgagatcgtc acgttcgacg atccgcaagc tgcgccgagc ggcttcgcgt tcgatacgca gacggccttc gcgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BumuA.00166.a.A1.PS01418
PURIFICATION DATe: 3/9/2012
CONCENTRATION: 18.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMLKQRTI KSIVKTVGIG LHSGRKVELT LRPAAPGTGI VFSRVDLPTP VDIPASAMSI GDTRLASVLQ KDGARVSTIE HLMSACAGLG IDNLYVDVTA EEIPIMDGSA ASFVFLIQSA GIEEQNAPKR FIKVKKPVEV RDGDKFARLD PFFGFKLKFT IDFRHPAVDK TGQALEVDFA NTSYVREIAR ARTFGFAHEV EMMRELGLAR GGSMDNAIVL DEYRILNNDG LRYDDEFVKH KMLDAIGDLY VVGHPLLASY TAYKSGHGLN NALLRELLAH EDAYEIVTFD DPQAAPSGFA FDTQTAFA
Validated NT Sequence
ttattacgcg aaggccgtct gcgtatcgaa cgcgaagccg ctcggcgcag cttgcggatc gtcgaacgtg acgatctcgt aggcgtcctc gtgcgcgagc agttcgcgca gcagcgcgtt gttcagcccg tgccccgact tgtacgcggt gtacgacgcg agcagcggat ggccgaccac gtacagatcg ccgatcgcgt cgagcatctt gtgcttcacg aattcgtcgt cgtagcgcag gccgtcgttg ttcaggatcc ggtactcgtc gagcacgatc gcgttgtcca tgctgccgcc gcgcgccagg cccagttcgc gcatcatctc gacttcatgc gcgaagccga acgtgcgtgc acgcgcgatc tcgcgcacgt acgacgtgtt cgcgaagtcg acctcgagcg cctggcccgt cttgtcgacc gccggatgac ggaagtcgat cgtgaatttc agcttgaagc cgaagaacgg atcgagacgc gcgaacttgt cgccgtcgcg cacttcgacc ggtttcttga ccttgatgaa gcgcttcggc gcattctgct cttcgatgcc ggccgactgg atcaggaaca cgaacgatgc cgcgctgccg tccatgatcg ggatttcctc ggccgtcacg tcgacataca ggttgtcgat gccgagcccc gcgcacgcgg acatcaggtg ctcgatcgtc gacacgcgcg cgccatcctt ctgcagcacc gatgcgagcc gcgtgtcgcc gatcgacatc gcgctggccg gaatatcgac gggcgtgggc aagtcgacgc gcgaaaacac gatccccgtg cccggcgcgg cgggacggag cgtcagttcg accttgcggc ccgagtggag accgataccg acggtcttca cgatcgattt gatggtgcgc tgcttcagca tcgaaccagg accctgggtc tgagcttcca gggtacccat atga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKQRTIKS IVKTVGIGLH SGRKVELTLR PAAPGTGIVF SRVDLPTPVD IPASAMSIGD TRLASVLQKD GARVSTIEHL MSACAGLGID NLYVDVTAEE IPIMDGSAAS FVFLIQSAGI EEQNAPKRFI KVKKPVEVRD GDKFARLDPF FGFKLKFTID FRHPAVDKTG QALEVDFANT SYVREIARAR TFGFAHEVEM MRELGLARGG SMDNAIVLDE YRILNNDGLR YDDEFVKHKM LDAIGDLYVV GHPLLASYTA YKSGHGLNNA LLRELLAHED AYEIVTFDDP QAAPSGFAFD TQTAFA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCTGAAGCAG CGCACCATCA AATCGATCGT GAAGACCGTC GGTATCGGTC TCCACTCGGG CCGCAAGGTC GAACTGACGC TCCGTCCCGC CGCGCCGGGC ACGGGGATCG TGTTTTCGCG CGTCGACTTG CCCACGCCCG TCGATATTCC GGCCAGCGCG ATGTCGATCG GCGACACGCG GCTCGCATCG GTGCTGCAGA AGGATGGCGC GCGCGTGTCG ACGATCGAGC ACCTGATGTC CGCGTGCGCG GGGCTCGGCA TCGACAACCT GTATGTCGAC GTGACGGCCG AGGAAATCCC GATCATGGAC GGCAGCGCGG CATCGTTCGT GTTCCTGATC CAGTCGGCCG GCATCGAAGA GCAGAATGCG CCGAAGCGCT TCATCAAGGT CAAGAAACCG GTCGAAGTGC GCGACGGCGA CAAGTTCGCG CGTCTCGATC CGTTCTTCGG CTTCAAGCTG AAATTCACGA TCGACTTCCG TCATCCGGCG GTCGACAAGA CGGGCCAGGC GCTCGAGGTC GACTTCGCGA ACACGTCGTA CGTGCGCGAG ATCGCGCGTG CACGCACGTT CGGCTTCGCG CATGAAGTCG AGATGATGCG CGAACTGGGC CTGGCGCGCG GCGGCAGCAT GGACAACGCG ATCGTGCTCG ACGAGTACCG GATCCTGAAC AACGACGGCC TGCGCTACGA CGACGAATTC GTGAAGCACA AGATGCTCGA CGCGATCGGC GATCTGTACG TGGTCGGCCA TCCGCTGCTC GCGTCGTACA CCGCGTACAA GTCGGGGCAC GGGCTGAACA ACGCGCTGCT GCGCGAACTG CTCGCGCACG AGGACGCCTA CGAGATCGTC ACGTTCGACG ATCCGCAAGC TGCGCCGAGC GGCTTCGCGT TCGATACGCA GACGGCCTTC GCGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA