SSGCID: Target Page

BumuA.00163.a

Phenylalanyl-tRNA synthetase, alpha subunit

CENTER ID:	BumuA.00163.a
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00163.a.B1.GE39143	Full length( BumuA.00163.a )		1	345

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.1988
RefSeq:	YP_001579960.1
UniProt:	A9ABF5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTLEMMGSMD LDQIVADAQQ SFERAADITT LENEKARFLG KSGALTELLK GLGKLDPEAR KTEGARINVA KQQVEAALTA RRQALADALL NQRLAAEAID VTLPGRGAGT GTLHPVMRTW ERVEQIFRSI GFDVADGPEI ETDWYNFTSL NSPENHPARS MQDTFYVEGK DADGRPLLLR THTSPMQVRY ARMNRPPIKV IAPGRTYRVD SDATHSPMFN QVEGLWIDEN ISFADLKGVY TDFLKKFFER DDILVRFRPS YFPFTEPSAE IDMMFEHGKN AGKWLEISGS GQVHPTVIRN MGLDPERYIG FAFGSGLERL TMLRYGVQDL RLFFENDLRF LRQFA

NT Sequence

ATGACGTTGG AAATGATGGG ATCTATGGAT CTGGACCAGA TTGTCGCCGA CGCGCAGCAG TCCTTCGAAC GGGCTGCCGA CATCACCACG CTCGAAAACG AGAAAGCACG ATTTCTCGGC AAGTCGGGCG CGCTGACCGA GTTGCTGAAG GGCCTCGGCA AGCTCGATCC CGAAGCACGC AAGACCGAAG GCGCACGCAT CAACGTCGCG AAGCAGCAGG TTGAAGCCGC GCTGACCGCA CGCCGTCAGG CGCTTGCCGA CGCGCTGCTG AACCAGCGCC TCGCCGCCGA AGCGATCGAC GTCACGCTGC CCGGCCGCGG TGCGGGCACC GGCACGCTGC ACCCCGTGAT GCGCACGTGG GAGCGCGTCG AACAGATTTT CCGCTCGATC GGTTTCGATG TGGCCGACGG CCCCGAGATC GAGACCGACT GGTACAACTT CACGTCGCTG AACAGCCCGG AAAACCATCC GGCCCGGTCG ATGCAGGACA CCTTCTACGT CGAAGGCAAG GACGCCGACG GCCGCCCGCT GCTGCTGCGC ACGCACACGA GCCCGATGCA GGTGCGCTAC GCGCGGATGA ACCGTCCGCC GATCAAGGTG ATCGCGCCGG GCCGCACGTA CCGCGTCGAC AGCGACGCGA CGCATTCGCC GATGTTCAAC CAGGTCGAAG GGCTGTGGAT CGACGAGAAC ATCAGCTTTG CCGACCTCAA GGGCGTCTAT ACCGACTTCC TGAAAAAATT CTTCGAGCGC GACGACATCC TCGTGCGCTT CCGTCCGTCG TACTTCCCGT TCACGGAACC GTCGGCCGAG ATCGACATGA TGTTCGAGCA CGGCAAGAAC GCCGGCAAGT GGCTCGAGAT CTCTGGCTCG GGGCAGGTGC ATCCGACCGT GATCCGCAAC ATGGGCCTCG ATCCCGAGCG CTACATCGGC TTCGCGTTCG GCAGCGGCCT CGAGCGTCTG ACGATGCTGC GCTACGGCGT GCAGGATCTG CGCCTGTTCT TCGAGAACGA TCTGCGCTTC CTGCGCCAGT TCGCG

Details for BumuA.00163.a.B1.GE39143

HARVESTED ON:	5/6/2015
SEQUENCED ON:	5/6/2015
EXPECTED MW:	40kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMT LEMMGSMDLD QIVADAQQSF ERAADITTLE NEKARFLGKS GALTELLKGL GKLDPEARKT EGARINVAKQ QVEAALTARR QALADALLNQ RLAAEAIDVT LPGRGAGTGT LHPVMRTWER VEQIFRSIGF DVADGPEIET DWYNFTSLNS PENHPARSMQ DTFYVEGKDA DGRPLLLRTH TSPMQVRYAR MNRPPIKVIA PGRTYRVDSD ATHSPMFNQV EGLWIDENIS FADLKGVYTD FLKKFFERDD ILVRFRPSYF PFTEPSAEID MMFEHGKNAG KWLEISGSGQ VHPTVIRNMG LDPERYIGFA FGSGLERLTM LRYGVQDLRL FFEN

Validated NT Sequence

cgttctcgaa gaacaggcgc agatcctgca cgccgtagcg cagcatcgtc agacgctcga ggccgctgcc gaacgcgaag ccgatgtagc gctcgggatc gaggcccatg ttgcggatca cggtcggatg cacctgcccc gagccagaga tctcgagcca cttgccggcg ttcttgccgt gctcgaacat catgtcgatc tcggccgacg gttccgtgaa cgggaagtac gacggacgga agcgcacgag gatgtcgtcg cgctcgaaga attttttcag gaagtcggta tagacgccct tgaggtcggc aaagctgatg ttctcgtcga tccacagccc ttcgacctgg ttgaacatcg gcgaatgcgt cgcgtcgctg tcgacgcggt acgtgcggcc cggcgcgatc accttgatcg gcggacggtt catccgcgcg tagcgcacct gcatcgggct cgtgtgcgtg cgcagcagca gcgggcggcc gtcggcgtcc ttgccttcga cgtagaaggt gtcctgcatc gaccgggccg gatggttttc cgggctgttc agcgacgtga agttgtacca gtcggtctcg atctcggggc cgtcggccac atcgaaaccg atcgagcgga aaatctgttc gacgcgctcc cacgtgcgca tcacggggtg cagcgtgccg gtgcccgcac cgcggccggg cagcgtgacg tcgatcgctt cggcggcgag gcgctggttc agcagcgcgt cggcaagcgc ctgacggcgt gcggtcagcg cggcttcaac ctgctgcttc gcgacgttga tgcgtgcgcc ttcggtcttg cgtgcttcgg gatcgagctt gccgaggccc ttcagcaact cggtcagcgc gcccgacttg ccgagaaatc gtgctttctc gttttcgagc gtggtgatgt cggcagcccg ttcgaaggac tgctgcgcgt cggcgacaat ctggtccaga tccatagatc ccatcatttc caacgtcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgt tggaaatgat gggatctatg gatctggacc agattgtcgc cgacgcgcag cagtccttcg aacgggctgc cgacatcacc acgctcgaaa acgagaaagc acgatttctc ggcaagtcgg gcgcgctgac cgagttgctg aagggcctcg gcaagctcga tcccgaagca cgcaagaccg aaggcgcacg catcaacgtc gcgaagcagc aggttgaagc cgcgctgacc gcacgccgtc aggcgcttgc cgacgcgctg ctgaaccagc gcctcgccgc cgaagcgatc gacgtcacgc tgcccggccg cggtgcgggc accggcacgc tgcaccccgt gatgcgcacg tgggagcgcg tcgaacagat tttccgctcg atcggtttcg atgtggccga cggccccgag atcgagaccg actggtacaa cttcacgtcg ctgaacagcc cggaaaacca tccggcccgg tcgatgcagg acaccttcta cgtcgaaggc aaggacgccg acggccgccc gctgctgctg cgcacgcaca cgagcccgat gcaggtgcgc tacgcgcgga tgaaccgtcc gccgatcaag gtgatcgcgc cgggccgcac gtaccgcgtc gacagcgacg cgacgcattc gccgatgttc aaccaggtcg aagggctgtg gatcgacgag aacatcagct ttgccgacct caagggcgtc tataccgact tcctgaaaaa attcttcgag cgcgacgaca tcctcgtgcg cttccgtccg tcgtacttcc cgttcacgga accgtcggcc gagatcgaca tgatgttcga gcacggcaag aacgccggca agtggctcga gatctctggc tcggggcagg tgcatccgac cgtgatccgc aacatgggcc tcgatcccga gcgctacatc ggcttcgcgt tcggcagcgg cctcgagcgt ctgacgatgc tgcgctacgg cgtgcaggat ctgcgcctgt tcttcgagaa cgatctgcgc ttcctgcgcc agttcgcgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat