SSGCID: Target Page

BumuA.00154.a

Chorismate synthase

CENTER ID:	BumuA.00154.a
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00154.a.B1.GE39703	Full length( BumuA.00154.a )		1	366

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00154.a.B1.PW37870	Full length( BumuA.00154.a )		1	366

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.2003
RefSeq:	YP_001579974.1
UniProt:	A9ABG9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSGNTLGTLF TVTTFGESHG PAIGCVIDGC PPGMALTEAD IQAELDRRKP GTSRHVTQRQ EADEVEILSG VFEGVTTGTP IALLIRNTDQ RSKDYGNIVE TFRPGHADYT YWQKYGVRDY RGGGRSSARL TAPIVGAGAV AKKWLRERFG VEVRGYMSAL GEIDVPFVDW SHVRENPFFA PNAAIVPELE AYMDALRKDG DSIGARIDVV ASGVPVGWGE PVFDRLDADI AKAMMSINAV KGVEIGAGFD SVAQRGSVHG DELTPAGFVG NHAGGVLGGI STGQDITVSI AIKPTSSIRT PRRSITKDGQ PATVETFGRH DPCVGIRATP IAESMLALVL IDHALRHRAQ CGDVETQTPK IAGSAT

NT Sequence

ATGTCCGGCA ATACCCTCGG CACGCTTTTC ACTGTCACGA CCTTCGGCGA GTCGCACGGC CCCGCGATCG GCTGCGTGAT CGACGGCTGC CCGCCGGGCA TGGCGCTCAC CGAGGCGGAC ATCCAGGCCG AGCTCGATCG CCGCAAGCCG GGCACGTCGC GGCACGTCAC GCAGCGGCAG GAGGCCGACG AGGTCGAGAT CCTGTCCGGC GTGTTCGAAG GCGTGACGAC CGGCACGCCG ATCGCGCTGC TGATCCGCAA CACCGATCAG CGCAGCAAGG ACTACGGCAA CATCGTCGAG ACGTTCCGGC CCGGCCATGC GGACTACACG TACTGGCAGA AATACGGCGT GCGCGACTAT CGCGGCGGCG GCCGCTCGTC CGCGCGCCTG ACCGCGCCGA TCGTCGGCGC GGGCGCGGTC GCGAAAAAGT GGCTGCGCGA GCGCTTCGGC GTCGAGGTGC GCGGCTACAT GAGCGCGCTG GGCGAGATCG ACGTGCCGTT CGTCGACTGG TCGCACGTGC GCGAGAATCC GTTCTTCGCG CCGAATGCGG CGATCGTCCC GGAACTCGAG GCGTACATGG ATGCGCTGCG CAAGGACGGC GATTCGATCG GTGCGCGCAT CGACGTCGTC GCGTCGGGCG TGCCGGTCGG GTGGGGCGAG CCGGTGTTCG ACCGGCTCGA CGCCGACATT GCGAAGGCGA TGATGAGCAT CAACGCGGTG AAGGGCGTCG AGATCGGCGC GGGCTTCGAC AGCGTCGCGC AGCGCGGTTC GGTGCACGGC GACGAGCTGA CGCCGGCCGG TTTCGTCGGC AATCACGCGG GCGGCGTGCT CGGCGGGATT TCGACGGGGC AGGACATCAC GGTGTCGATT GCGATCAAGC CGACGTCGAG CATCCGTACG CCGCGCCGCT CGATCACGAA GGACGGCCAG CCGGCGACGG TCGAAACGTT CGGCCGCCAC GATCCGTGCG TCGGCATCCG TGCGACGCCG ATCGCCGAAT CGATGCTCGC ACTGGTGCTG ATCGACCATG CGCTGCGCCA CCGCGCGCAG TGCGGCGACG TCGAGACGCA GACGCCGAAG ATCGCCGGCA GCGCGACG

Details for BumuA.00154.a.B1.GE39703

HARVESTED ON:	12/3/2015
SEQUENCED ON:	12/7/2015
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS GNTLGTLFTV TTFGESHGPA IGCVIDGCPP GMALTEADIQ AELDRRKPGT SRHVTQRQEA DEVEILSGVF EGVTTGTPIA LLIRNTDQRS KDYGNIVETF RPGHADYTYW QKYGVRDYRG GGRSSARLTA PIVGAGAVAK KWLRERFGVE VRGYMSALGE IDVPFVDWSH VRENPFFAPN AAIVPELEAY MDALRKDGDS IGARIDVVAS GVPVGWGEPV FDRLDADIAK AMMSINAVKG VEIGAGFDSV AQRGSVHGDE LTPAGFVGNH AGGVLGGIST GQDITVSIAI KPTSSIRTPR RSITKDGQPA TVETFGRHDP CVGIRATPIA ESMLALVLID HALRHRAQCG DVETQTPKIA GSAT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg gcaataccct cggcacgctt ttcactgtca cgaccttcgg cgagtcgcac ggccccgcga tcggctgcgt gatcgacggc tgcccgccgg gcatggcgct caccgaggcg gacatccagg ccgagctcga tcgccgcaag ccgggcacgt cgcggcacgt cacgcagcgg caggaggccg acgaggtcga gatcctgtcc ggcgtgttcg aaggcgtgac gaccggcacg ccgatcgcgc tgctgatccg caacaccgat cagcgcagca aggactacgg caacatcgtc gagacgttcc ggcccggcca tgcggactac acgtactggc agaaatacgg cgtgcgcgac tatcgcggcg gcggccgctc gtccgcgcgc ctgaccgcgc cgatcgtcgg cgcgggcgcg gtcgcgaaaa agtggctgcg cgagcgcttc ggcgtcgagg tgcgcggcta catgagcgcg ctgggcgaga tcgacgtgcc gttcgtcgac tggtcgcacg tgcgcgagaa tccgttcttc gcgccgaatg cggcgatcgt cccggaactc gaggcgtaca tggatgcgct gcgcaaggac ggcgattcga tcggtgcgcg catcgacgtc gtcgcgtcgg gcgtgccggt cgggtggggc gagccggtgt tcgaccggct cgacgccgac attgcgaagg cgatgatgag catcaacgcg gtgaagggcg tcgagatcgg cgcgggcttc gacagcgtcg cgcagcgcgg ttcggtgcac ggcgacgagc tgacgccggc cggtttcgtc ggcaatcacg cgggcggcgt gctcggcggg atttcgacgg ggcaggacat cacggtgtcg attgcgatca agccgacgtc gagcatccgt acgccgcgcc gctcgatcac gaaggacggc cagccggcga cggtcgaaac gttcggccgc cacgatccgt gcgtcggcat ccgtgcgacg ccgatcgccg aatcgatgct cgcactggtg ctgatcgacc atgcgctgcg ccaccgcgcg cagtgcggcg acgtcgagac gcagacgccg aagatcgccg gcagcgcgac gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BumuA.00154.a.B1.PW37870

PURIFICATION DATe:	3/2/2016
CONCENTRATION:	39mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	19
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG GCAATACCCT CGGCACGCTT TTCACTGTCA CGACCTTCGG CGAGTCGCAC GGCCCCGCGA TCGGCTGCGT GATCGACGGC TGCCCGCCGG GCATGGCGCT CACCGAGGCG GACATCCAGG CCGAGCTCGA TCGCCGCAAG CCGGGCACGT CGCGGCACGT CACGCAGCGG CAGGAGGCCG ACGAGGTCGA GATCCTGTCC GGCGTGTTCG AAGGCGTGAC GACCGGCACG CCGATCGCGC TGCTGATCCG CAACACCGAT CAGCGCAGCA AGGACTACGG CAACATCGTC GAGACGTTCC GGCCCGGCCA TGCGGACTAC ACGTACTGGC AGAAATACGG CGTGCGCGAC TATCGCGGCG GCGGCCGCTC GTCCGCGCGC CTGACCGCGC CGATCGTCGG CGCGGGCGCG GTCGCGAAAA AGTGGCTGCG CGAGCGCTTC GGCGTCGAGG TGCGCGGCTA CATGAGCGCG CTGGGCGAGA TCGACGTGCC GTTCGTCGAC TGGTCGCACG TGCGCGAGAA TCCGTTCTTC GCGCCGAATG CGGCGATCGT CCCGGAACTC GAGGCGTACA TGGATGCGCT GCGCAAGGAC GGCGATTCGA TCGGTGCGCG CATCGACGTC GTCGCGTCGG GCGTGCCGGT CGGGTGGGGC GAGCCGGTGT TCGACCGGCT CGACGCCGAC ATTGCGAAGG CGATGATGAG CATCAACGCG GTGAAGGGCG TCGAGATCGG CGCGGGCTTC GACAGCGTCG CGCAGCGCGG TTCGGTGCAC GGCGACGAGC TGACGCCGGC CGGTTTCGTC GGCAATCACG CGGGCGGCGT GCTCGGCGGG ATTTCGACGG GGCAGGACAT CACGGTGTCG ATTGCGATCA AGCCGACGTC GAGCATCCGT ACGCCGCGCC GCTCGATCAC GAAGGACGGC CAGCCGGCGA CGGTCGAAAC GTTCGGCCGC CACGATCCGT GCGTCGGCAT CCGTGCGACG CCGATCGCCG AATCGATGCT CGCACTGGTG CTGATCGACC ATGCGCTGCG CCACCGCGCG CAGTGCGGCG ACGTCGAGAC GCAGACGCCG AAGATCGCCG GCAGCGCGAC GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT