BumuA.00149.b

Cyclopropane-fatty-acyl-phospholipid synthase

CENTER ID: BumuA.00149.b
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00149.b.B2.GE39702 Mature protein( BumuA.00149.b ) 16 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.6142
RefSeq: YP_001585785.1
UniProt: A0A0H3KR27

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTLLRLLSSA SQPAMPLSAR LFIALLRRIR DGHLTLVTPE GAQHVFGDPH CQPAATLQIR DWRACRAILR AGDIGFAEAY RADRIDTPDL VALLRLAIRN QPVIAKPVTG GRVARVWYAL RHRLRMNTRA GSRRNIHAHY DLGNDFYRLW LDDTWTYSSA CFDGDPQRSL ADAQTAKYQR IVDALGLRAG MRVLEIGCGW GGFAVHAARQ GIHVHGVTIS HAQYALAQER VARDGLSDRV TFDLRDYRDV EGQYDAIVSI EMFEAVGESF WPVYFNTLRQ RLKPGARALI QSITIVESQF DAYRASSDFI REFIFPGGML PSPARFVDAA RRARLVAKPV FAFGDDYART LRTWRSSFDA HVDSVRAQRF DETFIRTWRL YLAYCEAGFA ERRTDVMHFI VSAHD
NT Sequence
ATGACCTTGC TGCGCCTGCT ATCCTCCGCA TCACAACCGG CGATGCCGCT GTCGGCCCGG TTGTTCATCG CGCTTCTCCG GCGGATCCGC GACGGTCACC TGACGCTCGT GACGCCCGAA GGCGCGCAGC ATGTGTTCGG CGATCCGCAC TGCCAGCCGG CCGCGACGCT GCAGATCCGC GACTGGCGCG CGTGCCGTGC GATCCTGCGC GCGGGCGACA TCGGCTTCGC GGAAGCGTAT CGCGCCGACC GGATCGACAC GCCGGATCTC GTCGCGCTGC TTCGCCTTGC GATCCGCAAC CAGCCGGTGA TCGCGAAGCC GGTGACGGGC GGCCGCGTGG CACGCGTGTG GTACGCGCTG CGCCATCGGC TACGGATGAA TACGCGAGCC GGCAGCCGCC GCAACATCCA TGCGCACTAT GACCTCGGCA ACGACTTCTA CCGGTTGTGG CTCGACGACA CCTGGACGTA TTCGAGCGCC TGCTTCGACG GCGACCCGCA GCGGTCGCTT GCCGACGCGC AAACGGCGAA GTATCAGCGC ATCGTCGATG CGCTCGGGCT GCGCGCCGGC ATGCGCGTGC TGGAGATCGG CTGCGGCTGG GGCGGCTTCG CGGTGCACGC TGCACGGCAA GGCATTCATG TACATGGCGT CACCATCTCG CACGCCCAAT ACGCGCTGGC CCAAGAGCGC GTCGCGCGCG ACGGGCTGTC GGATCGCGTC ACATTCGACT TGCGCGACTA TCGCGACGTC GAGGGCCAAT ACGACGCAAT CGTGTCGATC GAGATGTTCG AGGCCGTGGG CGAATCGTTC TGGCCGGTAT ACTTCAACAC GCTCAGGCAG CGCCTGAAGC CCGGCGCCCG CGCGCTGATT CAATCGATCA CGATCGTCGA GTCGCAATTC GACGCGTACC GCGCGTCGAG CGACTTCATT CGCGAGTTCA TCTTTCCCGG CGGAATGTTG CCGAGCCCCG CGCGCTTCGT CGATGCCGCG CGACGCGCGC GGCTCGTCGC GAAACCGGTG TTCGCGTTCG GCGACGACTA CGCACGCACG CTGCGCACCT GGCGATCCTC GTTCGATGCC CACGTCGACT CGGTGCGCGC GCAACGGTTC GACGAAACGT TCATTCGCAC CTGGCGGCTC TATCTCGCCT ATTGCGAAGC AGGATTCGCC GAACGGCGCA CCGACGTGAT GCACTTCATC GTGTCGGCGC ACGAC
Details for BumuA.00149.b.B2.GE39702
HARVESTED ON: 12/3/2015
SEQUENCED ON: 12/7/2015
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMP LSARLFIALL RRIRDGHLTL VTPEGAQHVF GDPHCQPAAT LQIRDWRACR AILRAGDIGF AEAYRADRID TPDLVALLRL AIRNQPVIAK PVTGGRVARV WYALRHRLRM NTRAGSRRNI HAHYDLGNDF YRLWLDDTWT YSSACFDGDP QRSLADAQTA KYQRIVDALG LRAGMRVLEI GCGWGGFAVH AARQGIHVHG VTISHAQYAL AQERVARDGL SDRVTFDLRD YRDVEGQYDA IVSIEMFEAV GESFWPVYFN TLRQRLKPGA RALIQSITIV ESQFDAYRAS SDFIREFIFP GGMLPSPARF VDAARRARLV AKPVFAFGDD YARTLRTWRS SFDAHVDSVR AQRFDETFIR TWRLYLAYCE AGFAERRT
Validated NT Sequence
tcggtgcgcc gttcggcgaa tcctgcttcg caataggcga gatagagccg ccaggtgcga atgaacgttt cgtcgaaccg ttgcgcgcgc accgagtcga cgtgggcatc gaacgaggat cgccaggtgc gcagcgtgcg tgcgtagtcg tcgccgaacg cgaacaccgg tttcgcgacg agccgcgcgc gtcgcgcggc atcgacgaag cgcgcggggc tcggcaacat tccgccggga aagatgaact cgcgaatgaa gtcgctcgac gcgcggtacg cgtcgaattg cgactcgacg atcgtgatcg attgaatcag cgcgcgggcg ccgggcttca ggcgctgcct gagcgtgttg aagtataccg gccagaacga ttcgcccacg gcctcgaaca tctcgatcga cacgattgcg tcgtattggc cctcgacgtc gcgatagtcg cgcaagtcga atgtgacgcg atccgacagc ccgtcgcgcg cgacgcgctc ttgggccagc gcgtattggg cgtgcgagat ggtgacgcca tgtacatgaa tgccttgccg tgcagcgtgc accgcgaagc cgccccagcc gcagccgatc tccagcacgc gcatgccggc gcgcagcccg agcgcatcga cgatgcgctg atacttcgcc gtttgcgcgt cggcaagcga ccgctgcggg tcgccgtcga agcaggcgct cgaatacgtc caggtgtcgt cgagccacaa ccggtagaag tcgttgccga ggtcatagtg cgcatggatg ttgcggcggc tgccggctcg cgtattcatc cgtagccgat ggcgcagcgc gtaccacacg cgtgccacgc ggccgcccgt caccggcttc gcgatcaccg gctggttgcg gatcgcaagg cgaagcagcg cgacgagatc cggcgtgtcg atccggtcgg cgcgatacgc ttccgcgaag ccgatgtcgc ccgcgcgcag gatcgcacgg cacgcgcgcc agtcgcggat ctgcagcgtc gcggccggct ggcagtgcgg atcgccgaac acatgctgcg cgccttcggg cgtcacgagc gtcaggtgac cgtcgcggat ccgccggaga agcgcgatga acaaccgggc cgacagcggc atatggtggt ggtggtggtg agccatgg
Expected Protein Sequence
MAHHHHHHPL SARLFIALLR RIRDGHLTLV TPEGAQHVFG DPHCQPAATL QIRDWRACRA ILRAGDIGFA EAYRADRIDT PDLVALLRLA IRNQPVIAKP VTGGRVARVW YALRHRLRMN TRAGSRRNIH AHYDLGNDFY RLWLDDTWTY SSACFDGDPQ RSLADAQTAK YQRIVDALGL RAGMRVLEIG CGWGGFAVHA ARQGIHVHGV TISHAQYALA QERVARDGLS DRVTFDLRDY RDVEGQYDAI VSIEMFEAVG ESFWPVYFNT LRQRLKPGAR ALIQSITIVE SQFDAYRASS DFIREFIFPG GMLPSPARFV DAARRARLVA KPVFAFGDDY ARTLRTWRSS FDAHVDSVRA QRFDETFIRT WRLYLAYCEA GFAERRTDVM HFIVSAHD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccgctgt cggcccggtt gttcatcgcg cttctccggc ggatccgcga cggtcacctg acgctcgtga cgcccgaagg cgcgcagcat gtgttcggcg atccgcactg ccagccggcc gcgacgctgc agatccgcga ctggcgcgcg tgccgtgcga tcctgcgcgc gggcgacatc ggcttcgcgg aagcgtatcg cgccgaccgg atcgacacgc cggatctcgt cgcgctgctt cgccttgcga tccgcaacca gccggtgatc gcgaagccgg tgacgggcgg ccgcgtggca cgcgtgtggt acgcgctgcg ccatcggcta cggatgaata cgcgagccgg cagccgccgc aacatccatg cgcactatga cctcggcaac gacttctacc ggttgtggct cgacgacacc tggacgtatt cgagcgcctg cttcgacggc gacccgcagc ggtcgcttgc cgacgcgcaa acggcgaagt atcagcgcat cgtcgatgcg ctcgggctgc gcgccggcat gcgcgtgctg gagatcggct gcggctgggg cggcttcgcg gtgcacgctg cacggcaagg cattcatgta catggcgtca ccatctcgca cgcccaatac gcgctggccc aagagcgcgt cgcgcgcgac gggctgtcgg atcgcgtcac attcgacttg cgcgactatc gcgacgtcga gggccaatac gacgcaatcg tgtcgatcga gatgttcgag gccgtgggcg aatcgttctg gccggtatac ttcaacacgc tcaggcagcg cctgaagccc ggcgcccgcg cgctgattca atcgatcacg atcgtcgagt cgcaattcga cgcgtaccgc gcgtcgagcg acttcattcg cgagttcatc tttcccggcg gaatgttgcc gagccccgcg cgcttcgtcg atgccgcgcg acgcgcgcgg ctcgtcgcga aaccggtgtt cgcgttcggc gacgactacg cacgcacgct gcgcacctgg cgatcctcgt tcgatgccca cgtcgactcg gtgcgcgcgc aacggttcga cgaaacgttc attcgcacct ggcggctcta tctcgcctat tgcgaagcag gattcgccga acggcgcacc gacgtgatgc acttcatcgt gtcggcgcac gactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat