SSGCID: Target Page

BumuA.00149.a

Cyclopropane-fatty-acyl-phospholipid synthase

CENTER ID:	BumuA.00149.a
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00149.a.B1.GE39701	Full length( BumuA.00149.a )		1	366

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.6138
RefSeq:	YP_001585781.1
UniProt:	A0A0H3KV38

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTATTSQSSP AAIAAPQDAW LIRCCERGWL PDALIRAGMR RLMRQRLRDA HAHDGEPRAA AVDALVHELR ASPIAIETHA ANTQHYELPG SFFEAHLGPR RKYSCGYYPN GNETLSQGEE AMLALYAQRA DLADGQRILD LGCGWGSLSL WLAERYPAAQ IVGLSNSYGQ REFIERCAAL RRLTNLRIVT GNVVDFDFDP AEAGFDRVLS IEMFEHMKNY GQLLAKIARW MDDDGKLFVH IFAHKLAAYH FAVHDDTDWM SRHFFTGGTM PSADLLLRFQ DDVRIARQWW LDGTHYARTA NQWLASLDAA RPRIMPIFDT VYGADARIWF QRWRMFYMAV AELFGYADGQ EWGVAHYLFD KRGGRA

NT Sequence

ATGACCGCTA CCACCTCGCA ATCGTCGCCT GCCGCGATCG CCGCCCCCCA GGATGCCTGG CTGATTCGCT GCTGCGAGCG CGGCTGGCTG CCCGACGCGC TGATCCGGGC CGGGATGCGC AGGCTGATGC GGCAGCGGCT GCGCGACGCG CACGCACACG ACGGCGAGCC GCGTGCGGCG GCCGTCGACG CGCTCGTGCA CGAACTACGC GCGAGCCCGA TCGCGATCGA AACCCACGCG GCCAATACCC AGCATTACGA ACTGCCCGGC AGCTTCTTCG AGGCCCATCT CGGCCCACGG CGCAAATATT CGTGCGGCTA TTACCCGAAC GGCAACGAAA CGCTGTCGCA AGGCGAGGAG GCCATGCTCG CGCTGTACGC GCAGCGCGCC GATCTCGCGG ACGGCCAGCG TATTCTCGAC CTGGGCTGCG GCTGGGGTTC GCTGTCGCTG TGGCTCGCCG AGCGGTATCC GGCCGCGCAG ATCGTCGGAC TCTCGAATTC GTACGGTCAG CGCGAGTTCA TCGAACGATG CGCGGCGCTG CGCAGGCTGA CGAATCTGCG GATCGTGACC GGCAACGTGG TCGACTTCGA CTTCGATCCG GCGGAAGCCG GCTTCGATCG CGTGCTGTCG ATCGAGATGT TCGAGCACAT GAAGAACTAC GGACAGCTGC TCGCGAAAAT CGCGCGCTGG ATGGACGACG ATGGCAAGCT GTTCGTGCAC ATCTTCGCGC ACAAGCTCGC CGCCTATCAC TTCGCGGTGC ACGACGACAC CGACTGGATG TCGCGCCACT TCTTCACGGG CGGGACGATG CCGTCGGCCG ATCTGCTGCT GCGGTTTCAG GACGATGTGC GAATCGCGCG CCAGTGGTGG CTCGACGGCA CTCATTACGC GCGCACCGCA AATCAGTGGC TCGCGTCGCT CGACGCGGCG CGCCCGCGGA TCATGCCCAT TTTCGACACC GTCTACGGCG CCGACGCGCG CATCTGGTTC CAGCGCTGGC GGATGTTCTA CATGGCCGTC GCCGAGCTGT TCGGCTACGC CGACGGGCAG GAATGGGGCG TCGCCCACTA CCTGTTCGAC AAGCGCGGGG GGCGCGCA

Details for BumuA.00149.a.B1.GE39701

HARVESTED ON:	12/3/2015
SEQUENCED ON:	12/7/2015
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	96

Validated AA Sequence

MAHHHHHHMT ATTSQSSPAA IAAPQDAWLI RCCERGWLPD ALIRAGMRRL MRQRLRDAHA HDGEPRAAAV DALVHELRAS PIAIETHAAN TQHYELPGSF FEAHLGPRRK YSCGYYPNGN ETLSQGEEAM LALYAQRADL ADGQRILDLG CGWGSLSLWL AERYPAAQIV GLSNSYGQRE FIERCAALRR LTNLRIVTGN VVDFDFDPAE AGFDRVLSIE MFEHMKNYGQ LLAKIARWMD DDGKLFVHIF AHKLAAYHFA VHDDTDWMSR HFFTGGTMPS ADLLLRFQDD VRIARQWWLD GTHYARTANQ WLASLDAARP RIMPIFDTVY GADARIWFQR WRMFYMAVAE LFXXADGQEW

Validated NT Sequence

ccccattcct gcccgtcggc gtnnncgaac agctcggcga cggccatgta gaacatccgc cagcgctgga accagatgcg cgcgtcggcg ccgtagacgg tgtcgaaaat gggcatgatc cgcgggcgcg ccgcgtcgag cgacgcgagc cactgatttg cggtgcgcgc gtaatgagtg ccgtcgagcc accactggcg cgcgattcgc acatcgtcct gaaaccgcag cagcagatcg gccgacggca tcgtcccgcc cgtgaagaag tggcgcgaca tccagtcggt gtcgtcgtgc accgcgaagt gataggcggc gagcttgtgc gcgaagatgt gcacgaacag cttgccatcg tcgtccatcc agcgcgcgat tttcgcgagc agctgtccgt agttcttcat gtgctcgaac atctcgatcg acagcacgcg atcgaagccg gcttccgccg gatcgaagtc gaagtcgacc acgttgccgg tcacgatccg cagattcgtc agcctgcgca gcgccgcgca tcgttcgatg aactcgcgct gaccgtacga attcgagagt ccgacgatct gcgcggccgg ataccgctcg gcgagccaca gcgacagcga accccagccg cagcccaggt cgagaatacg ctggccgtcc gcgagatcgg cgcgctgcgc gtacagcgcg agcatggcct cctcgccttg cgacagcgtt tcgttgccgt tcgggtaata gccgcacgaa tatttgcgcc gtgggccgag atgggcctcg aagaagctgc cgggcagttc gtaatgctgg gtattggccg cgtgggtttc gatcgcgatc gggctcgcgc gtagttcgtg cacgagcgcg tcgacggccg ccgcacgcgg ctcgccgtcg tgtgcgtgcg cgtcgcgcag ccgctgccgc atcagcctgc gcatcccggc ccggatcagc gcgtcgggca gccagccgcg ctcgcagcag cgaatcagcc aggcatcctg gggggcggcg atcgcggcag gcgacgattg cgaggtggta gcggtcatat ggtggtggtg gtggtgagcc atg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg ctaccacctc gcaatcgtcg cctgccgcga tcgccgcccc ccaggatgcc tggctgattc gctgctgcga gcgcggctgg ctgcccgacg cgctgatccg ggccgggatg cgcaggctga tgcggcagcg gctgcgcgac gcgcacgcac acgacggcga gccgcgtgcg gcggccgtcg acgcgctcgt gcacgaacta cgcgcgagcc cgatcgcgat cgaaacccac gcggccaata cccagcatta cgaactgccc ggcagcttct tcgaggccca tctcggccca cggcgcaaat attcgtgcgg ctattacccg aacggcaacg aaacgctgtc gcaaggcgag gaggccatgc tcgcgctgta cgcgcagcgc gccgatctcg cggacggcca gcgtattctc gacctgggct gcggctgggg ttcgctgtcg ctgtggctcg ccgagcggta tccggccgcg cagatcgtcg gactctcgaa ttcgtacggt cagcgcgagt tcatcgaacg atgcgcggcg ctgcgcaggc tgacgaatct gcggatcgtg accggcaacg tggtcgactt cgacttcgat ccggcggaag ccggcttcga tcgcgtgctg tcgatcgaga tgttcgagca catgaagaac tacggacagc tgctcgcgaa aatcgcgcgc tggatggacg acgatggcaa gctgttcgtg cacatcttcg cgcacaagct cgccgcctat cacttcgcgg tgcacgacga caccgactgg atgtcgcgcc acttcttcac gggcgggacg atgccgtcgg ccgatctgct gctgcggttt caggacgatg tgcgaatcgc gcgccagtgg tggctcgacg gcactcatta cgcgcgcacc gcaaatcagt ggctcgcgtc gctcgacgcg gcgcgcccgc ggatcatgcc cattttcgac accgtctacg gcgccgacgc gcgcatctgg ttccagcgct ggcggatgtt ctacatggcc gtcgccgagc tgttcggcta cgccgacggg caggaatggg gcgtcgccca ctacctgttc gacaagcgcg gggggcgcgc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat