BumuA.00148.b

Integrase family protein

CENTER ID: BumuA.00148.b
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00148.b.B1.GE39696 Full length( BumuA.00148.b ) 1 304
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: YP_001580464.1
UniProt: A9ADX9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRSDMERYI QLRRSAGFKM ANESLMLSGF VAIAEKHGDQ HIRIDRVLTW AGQAPSSAQR YRRLNVIRHF AQALQAEDDR HQVPPRTAFG NSRNRRPPPY IFSQEEIAKL IEAAGQLKPT GSVRPMMFAT LFGLLAATGL RISEALRLRL QDITVDGLMI LETKFNKSRL VPLHDTVRAA LERYLVVRTR TATSTDALFV GTNGKAPGYR GVKDVFVRLV TVMGLQQARN GGLPHIHDLR HTLAVRSLEQ FRGDRNAISR HMTALSTYLG HTKVTDTYWY LEATPVLLGQ IAEAAETLHR EQDL
NT Sequence
ATGTTGAGAT CCGACATGGA GCGCTATATC CAGCTACGGC GGAGCGCTGG TTTCAAGATG GCAAACGAAT CGCTGATGCT GAGCGGCTTT GTTGCTATTG CCGAAAAGCA TGGTGATCAA CACATTCGTA TCGACCGAGT GCTGACGTGG GCGGGGCAAG CACCGTCATC AGCACAGCGG TACCGGCGTC TCAACGTCAT CCGGCACTTT GCGCAAGCAC TTCAGGCTGA AGATGATCGG CACCAGGTGC CACCACGTAC TGCGTTTGGC AACTCCAGGA ATCGACGACC GCCGCCCTAT ATCTTCAGCC AAGAGGAGAT TGCCAAACTC ATTGAAGCTG CCGGTCAGTT GAAACCGACT GGCTCTGTCC GGCCGATGAT GTTTGCGACG CTATTTGGAC TTCTGGCTGC GACAGGCTTG CGCATCTCGG AAGCCTTGAG GTTACGGTTG CAGGACATTA CCGTCGATGG CTTGATGATC CTGGAAACGA AATTCAACAA GAGTCGGTTG GTTCCGCTGC ACGACACCGT CCGCGCTGCA TTGGAGCGGT ATCTCGTGGT GCGCACACGC ACTGCGACGA GCACCGATGC TTTGTTTGTC GGCACCAATG GGAAAGCGCC TGGCTACAGG GGTGTGAAGG ATGTGTTCGT TCGTCTGGTC ACTGTTATGG GGCTGCAACA GGCCCGAAAT GGCGGTCTTC CCCACATCCA CGATCTTCGG CACACACTGG CCGTTCGTAG CCTGGAACAA TTCCGTGGTG ACCGGAATGC CATTTCTCGC CATATGACGG CATTGAGCAC CTACCTTGGG CACACCAAAG TAACGGACAC CTACTGGTAC CTTGAGGCAA CGCCGGTCTT GCTTGGGCAA ATTGCTGAAG CAGCAGAAAC GCTGCATAGG GAGCAAGACC TA
Details for BumuA.00148.b.B1.GE39696
HARVESTED ON: 12/3/2015
SEQUENCED ON: 12/7/2015
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML RSDMERYIQL RRSAGFKMAN ESLMLSGFVA IAEKHGDQHI RIDRVLTWAG QAPSSAQRYR RLNVIRHFAQ ALQAEDDRHQ VPPRTAFGNS RNRRPPPYIF SQEEIAKLIE AAGQLKPTGS VRPMMFATLF GLLAATGLRI SEALRLRLQD ITVDGLMILE TKFNKSRLVP LHDTVRAALE RYLVVRTRTA TSTDALFVGT NGKAPGYRGV KDVFVRLVTV MGLQQARNGG LPHIHDLRHT LAVRSLEQFR GDRNAISRHM TALSTYLGHT KVTDTYWYLE ATPVLLGQIA EAAEXXHREQ DL
Validated NT Sequence
ttactcactt ataggtcttg ctccctatgn nnnnnttctg ctgcttcagc aatttgccca agcaagaccg gcgttgcctc aaggtaccag taggtgtccg ttactttggt gtgcccaagg taggtgctca atgccgtcat atggcgagaa atggcattcc ggtcaccacg gaattgttcc aggctacgaa cggccagtgt gtgccgaaga tcgtggatgt ggggaagacc gccatttcgg gcctgttgca gccccataac agtgaccaga cgaacgaaca catccttcac acccctgtag ccaggcgctt tcccattggt gccgacaaac aaagcatcgg tgctcgtcgc agtgcgtgtg cgcaccacga gataccgctc caatgcagcg cggacggtgt cgtgcagcgg aaccaaccga ctcttgttga atttcgtttc caggatcatc aagccatcga cggtaatgtc ctgcaaccgt aacctcaagg cttccgagat gcgcaagcct gtcgcagcca gaagtccaaa tagcgtcgca aacatcatcg gccggacaga gccagtcggt ttcaactgac cggcagcttc aatgagtttg gcaatctcct cttggctgaa gatatagggc ggcggtcgtc gattcctgga gttgccaaac gcagtacgtg gtggcacctg gtgccgatca tcttcagcct gaagtgcttg cgcaaagtgc cggatgacgt tgagacgccg gtaccgctgt gctgatgacg gtgcttgccc cgcccacgtc agcactcggt cgatacgaat gtgttgatca ccatgctttt cggcaatagc aacaaagccg ctcagcatca gcgattcgtt tgccatcttg aaaccagcgc tccgccgtag ctggatatag cgctccatgt cggatctcaa catatggtgg tggtggtggt gagccatggt
Expected Protein Sequence
MAHHHHHHML RSDMERYIQL RRSAGFKMAN ESLMLSGFVA IAEKHGDQHI RIDRVLTWAG QAPSSAQRYR RLNVIRHFAQ ALQAEDDRHQ VPPRTAFGNS RNRRPPPYIF SQEEIAKLIE AAGQLKPTGS VRPMMFATLF GLLAATGLRI SEALRLRLQD ITVDGLMILE TKFNKSRLVP LHDTVRAALE RYLVVRTRTA TSTDALFVGT NGKAPGYRGV KDVFVRLVTV MGLQQARNGG LPHIHDLRHT LAVRSLEQFR GDRNAISRHM TALSTYLGHT KVTDTYWYLE ATPVLLGQIA EAAETLHREQ DL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga gatccgacat ggagcgctat atccagctac ggcggagcgc tggtttcaag atggcaaacg aatcgctgat gctgagcggc tttgttgcta ttgccgaaaa gcatggtgat caacacattc gtatcgaccg agtgctgacg tgggcggggc aagcaccgtc atcagcacag cggtaccggc gtctcaacgt catccggcac tttgcgcaag cacttcaggc tgaagatgat cggcaccagg tgccaccacg tactgcgttt ggcaactcca ggaatcgacg accgccgccc tatatcttca gccaagagga gattgccaaa ctcattgaag ctgccggtca gttgaaaccg actggctctg tccggccgat gatgtttgcg acgctatttg gacttctggc tgcgacaggc ttgcgcatct cggaagcctt gaggttacgg ttgcaggaca ttaccgtcga tggcttgatg atcctggaaa cgaaattcaa caagagtcgg ttggttccgc tgcacgacac cgtccgcgct gcattggagc ggtatctcgt ggtgcgcaca cgcactgcga cgagcaccga tgctttgttt gtcggcacca atgggaaagc gcctggctac aggggtgtga aggatgtgtt cgttcgtctg gtcactgtta tggggctgca acaggcccga aatggcggtc ttccccacat ccacgatctt cggcacacac tggccgttcg tagcctggaa caattccgtg gtgaccggaa tgccatttct cgccatatga cggcattgag cacctacctt gggcacacca aagtaacgga cacctactgg taccttgagg caacgccggt cttgcttggg caaattgctg aagcagcaga aacgctgcat agggagcaag acctatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat