BumuA.00138.a

Predicted metal-dependent hydrolase

CENTER ID: BumuA.00138.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00138.a.B1.GE39692 Full length( BumuA.00138.a ) 1 243
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00138.a.B1.PS37871 Full length( BumuA.00138.a ) 1 243

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.1169
RefSeq: YP_001579188.1
UniProt: A0A0H3KG77

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTQALFRDD AYLAHCEAIV EAVGDDGIRL DRTVFYPLGG GQAGDAGTLT LPDGSTIAIA DTRKAKFDGA TPDDALHVPA PGQDADVAAL APGTRVVATI DWARRYRHMR LHTAAHLMCA VLPYAVDGCS VTADYVRLDF ATSDAIDRDD VERRLAELVR GAHPVTTEWI TDEEMAARPE LVRTMSVKPP MGLGRVRLLR IDGVDLQPCG GTHVRNTSEI GALRVAKLEK KSARTRRLVL EFA
NT Sequence
ATGACGACAC AAGCGCTGTT TCGCGACGAC GCCTATCTCG CGCACTGCGA GGCGATCGTC GAGGCCGTCG GCGACGACGG CATCCGGCTC GACCGCACGG TGTTCTATCC GCTCGGCGGC GGCCAGGCCG GCGACGCCGG CACGTTGACG CTGCCCGACG GCAGCACGAT CGCGATCGCC GACACGCGCA AGGCGAAGTT CGACGGCGCG ACGCCCGACG ACGCGCTCCA CGTGCCCGCG CCGGGGCAGG ACGCGGATGT CGCGGCGCTC GCACCGGGCA CGCGCGTGGT CGCGACGATC GACTGGGCGC GCCGCTATCG GCACATGCGG CTGCACACGG CCGCGCATCT GATGTGCGCG GTGCTGCCGT ACGCGGTCGA CGGCTGCAGC GTGACGGCCG ACTACGTGCG GCTCGACTTC GCGACGTCCG ATGCGATCGA TCGCGACGAC GTCGAGCGGC GCCTCGCGGA GCTCGTCCGC GGCGCGCATC CCGTCACGAC CGAATGGATC ACCGACGAAG AGATGGCGGC GCGGCCGGAA CTCGTGCGCA CGATGAGCGT GAAGCCGCCG ATGGGGCTCG GCCGCGTGCG GCTGCTGCGC ATCGACGGTG TCGATCTGCA GCCGTGCGGC GGCACGCACG TGCGCAACAC GTCGGAGATC GGTGCGCTGC GCGTCGCGAA GCTGGAAAAG AAGAGCGCGC GCACGCGGCG CCTCGTGCTG GAGTTCGCA
Details for BumuA.00138.a.B1.GE39692
HARVESTED ON: 12/3/2015
SEQUENCED ON: 12/7/2015
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT TQALFRDDAY LAHCEAIVEA VGDDGIRLDR TVFYPLGGGQ AGDAGTLTLP DGSTIAIADT RKAKFDGATP DDALHVPAPG QDADVAALAP GTRVVATIDW ARRYRHMRLH TAAHLMCAVL PYAVDGCSVT ADYVRLDFAT SDAIDRDDVE RRLAELVRGA HPVTTEWITD EEMAARPELV RTMSVKPPMG LGRVRLLRID GVDLQPCGGT HVRNTSEIGX XRVAKLEKKS ARTXAXRAGV XXK
Validated NT Sequence
cttactcact tnnnnngaac tccagcacgn ngcgccnncg tgcgcgcgct cttcttttcc agcttcgcga cgcgcannnc accgatctcc gacgtgttgc gcacgtgcgt gccgccgcac ggctgcagat cgacaccgtc gatgcgcagc agccgcacgc ggccgagccc catcggcggc ttcacgctca tcgtgcgcac gagttccggc cgcgccgcca tctcttcgtc ggtgatccat tcggtcgtga cgggatgcgc gccgcggacg agctccgcga ggcgccgctc gacgtcgtcg cgatcgatcg catcggacgt cgcgaagtcg agccgcacgt agtcggccgt cacgctgcag ccgtcgaccg cgtacggcag caccgcgcac atcagatgcg cggccgtgtg cagccgcatg tgccgatagc ggcgcgccca gtcgatcgtc gcgaccacgc gcgtgcccgg tgcgagcgcc gcgacatccg cgtcctgccc cggcgcgggc acgtggagcg cgtcgtcggg cgtcgcgccg tcgaacttcg ccttgcgcgt gtcggcgatc gcgatcgtgc tgccgtcggg cagcgtcaac gtgccggcgt cgccggcctg gccgccgccg agcggataga acaccgtgcg gtcgagccgg atgccgtcgt cgccgacggc ctcgacgatc gcctcgcagt gcgcgagata ggcgtcgtcg cgaaacagcg cttgtgtcgt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMT TQALFRDDAY LAHCEAIVEA VGDDGIRLDR TVFYPLGGGQ AGDAGTLTLP DGSTIAIADT RKAKFDGATP DDALHVPAPG QDADVAALAP GTRVVATIDW ARRYRHMRLH TAAHLMCAVL PYAVDGCSVT ADYVRLDFAT SDAIDRDDVE RRLAELVRGA HPVTTEWITD EEMAARPELV RTMSVKPPMG LGRVRLLRID GVDLQPCGGT HVRNTSEIGA LRVAKLEKKS ARTRRLVLEF A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga cacaagcgct gtttcgcgac gacgcctatc tcgcgcactg cgaggcgatc gtcgaggccg tcggcgacga cggcatccgg ctcgaccgca cggtgttcta tccgctcggc ggcggccagg ccggcgacgc cggcacgttg acgctgcccg acggcagcac gatcgcgatc gccgacacgc gcaaggcgaa gttcgacggc gcgacgcccg acgacgcgct ccacgtgccc gcgccggggc aggacgcgga tgtcgcggcg ctcgcaccgg gcacgcgcgt ggtcgcgacg atcgactggg cgcgccgcta tcggcacatg cggctgcaca cggccgcgca tctgatgtgc gcggtgctgc cgtacgcggt cgacggctgc agcgtgacgg ccgactacgt gcggctcgac ttcgcgacgt ccgatgcgat cgatcgcgac gacgtcgagc ggcgcctcgc ggagctcgtc cgcggcgcgc atcccgtcac gaccgaatgg atcaccgacg aagagatggc ggcgcggccg gaactcgtgc gcacgatgag cgtgaagccg ccgatggggc tcggccgcgt gcggctgctg cgcatcgacg gtgtcgatct gcagccgtgc ggcggcacgc acgtgcgcaa cacgtcggag atcggtgcgc tgcgcgtcgc gaagctggaa aagaagagcg cgcgcacgcg gcgcctcgtg ctggagttcg catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BumuA.00138.a.B1.PS37871
PURIFICATION DATe: 3/10/2016
CONCENTRATION: 31.2mg/ml
OBSERVED MW: 27kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT TQALFRDDAY LAHCEAIVEA VGDDGIRLDR TVFYPLGGGQ AGDAGTLTLP DGSTIAIADT RKAKFDGATP DDALHVPAPG QDADVAALAP GTRVVATIDW ARRYRHMRLH TAAHLMCAVL PYAVDGCSVT ADYVRLDFAT SDAIDRDDVE RRLAELVRGA HPVTTEWITD EEMAARPELV RTMSVKPPMG LGRVRLLRID GVDLQPCGGT HVRNTSEIGX XRVAKLEKKS ARTXAXRAGV XXK
Validated NT Sequence
cttactcact tnnnnngaac tccagcacgn ngcgccnncg tgcgcgcgct cttcttttcc agcttcgcga cgcgcannnc accgatctcc gacgtgttgc gcacgtgcgt gccgccgcac ggctgcagat cgacaccgtc gatgcgcagc agccgcacgc ggccgagccc catcggcggc ttcacgctca tcgtgcgcac gagttccggc cgcgccgcca tctcttcgtc ggtgatccat tcggtcgtga cgggatgcgc gccgcggacg agctccgcga ggcgccgctc gacgtcgtcg cgatcgatcg catcggacgt cgcgaagtcg agccgcacgt agtcggccgt cacgctgcag ccgtcgaccg cgtacggcag caccgcgcac atcagatgcg cggccgtgtg cagccgcatg tgccgatagc ggcgcgccca gtcgatcgtc gcgaccacgc gcgtgcccgg tgcgagcgcc gcgacatccg cgtcctgccc cggcgcgggc acgtggagcg cgtcgtcggg cgtcgcgccg tcgaacttcg ccttgcgcgt gtcggcgatc gcgatcgtgc tgccgtcggg cagcgtcaac gtgccggcgt cgccggcctg gccgccgccg agcggataga acaccgtgcg gtcgagccgg atgccgtcgt cgccgacggc ctcgacgatc gcctcgcagt gcgcgagata ggcgtcgtcg cgaaacagcg cttgtgtcgt catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMT TQALFRDDAY LAHCEAIVEA VGDDGIRLDR TVFYPLGGGQ AGDAGTLTLP DGSTIAIADT RKAKFDGATP DDALHVPAPG QDADVAALAP GTRVVATIDW ARRYRHMRLH TAAHLMCAVL PYAVDGCSVT ADYVRLDFAT SDAIDRDDVE RRLAELVRGA HPVTTEWITD EEMAARPELV RTMSVKPPMG LGRVRLLRID GVDLQPCGGT HVRNTSEIGA LRVAKLEKKS ARTRRLVLEF A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGA CACAAGCGCT GTTTCGCGAC GACGCCTATC TCGCGCACTG CGAGGCGATC GTCGAGGCCG TCGGCGACGA CGGCATCCGG CTCGACCGCA CGGTGTTCTA TCCGCTCGGC GGCGGCCAGG CCGGCGACGC CGGCACGTTG ACGCTGCCCG ACGGCAGCAC GATCGCGATC GCCGACACGC GCAAGGCGAA GTTCGACGGC GCGACGCCCG ACGACGCGCT CCACGTGCCC GCGCCGGGGC AGGACGCGGA TGTCGCGGCG CTCGCACCGG GCACGCGCGT GGTCGCGACG ATCGACTGGG CGCGCCGCTA TCGGCACATG CGGCTGCACA CGGCCGCGCA TCTGATGTGC GCGGTGCTGC CGTACGCGGT CGACGGCTGC AGCGTGACGG CCGACTACGT GCGGCTCGAC TTCGCGACGT CCGATGCGAT CGATCGCGAC GACGTCGAGC GGCGCCTCGC GGAGCTCGTC CGCGGCGCGC ATCCCGTCAC GACCGAATGG ATCACCGACG AAGAGATGGC GGCGCGGCCG GAACTCGTGC GCACGATGAG CGTGAAGCCG CCGATGGGGC TCGGCCGCGT GCGGCTGCTG CGCATCGACG GTGTCGATCT GCAGCCGTGC GGCGGCACGC ACGTGCGCAA CACGTCGGAG ATCGGTGCGC TGCGCGTCGC GAAGCTGGAA AAGAAGAGCG CGCGCACGCG GCGCCTCGTG CTGGAGTTCG CATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT