BumuA.00134.a

4-hydroxyphenylpyruvate dioxygenase

CENTER ID: BumuA.00134.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00134.a.B1.GE39691 Full length( BumuA.00134.a ) 1 375
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00134.a.B1.PS37893 Full length( BumuA.00134.a ) 1 375

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.4542
RefSeq: YP_001584199.1
UniProt: A9AKD4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGNPHPLSS DTPPVDDPAA NPLGMAGIEF VEFAAPVPDV LAQRFEQLGF KAIARHVSKA VTLYRQGQMH FLINAEPDSF AARYAEEYGM GVCAIGIRVA NARRSFERAI ELGAWAFEGE RVGVGELKIP AIQGIGDSHL YFVDRWRGRN GQRGGVGDIS IFDIDFRPID IATAHTDLDF AGTGLQQVDH FTQTVGAGRM QEWLDFYHDL LHFREIHEID AHWHVSEESR VMVSPCGALR IPVYEEGTRR TELMHAYLPD HPGEGVQHIA LATDDILASV DALRANGVEF IEPPARYYDE VDQRLPGHGV DLDALRRRAV LIDGEIGEDG VPRLFFQTFV KRRPGEIFFE IVQRKGHHGF GEGNLAALAR ARDAS
NT Sequence
ATGTCCGGCA ATCCCCATCC ACTGTCCAGC GATACGCCGC CCGTCGACGA TCCGGCCGCC AATCCGCTCG GGATGGCGGG GATCGAGTTC GTCGAGTTCG CGGCGCCGGT GCCGGACGTG CTCGCGCAGC GTTTCGAACA GCTCGGCTTC AAGGCGATCG CGCGGCACGT CAGCAAGGCC GTGACGCTGT ATCGGCAGGG GCAGATGCAT TTCCTGATCA ACGCTGAACC GGACTCGTTC GCCGCGCGCT ATGCGGAGGA ATACGGGATG GGAGTCTGCG CGATCGGCAT CCGCGTCGCG AACGCGCGGC GCTCGTTCGA ACGCGCGATC GAACTGGGCG CGTGGGCGTT CGAGGGCGAG CGCGTCGGCG TCGGTGAGCT GAAGATTCCG GCGATCCAGG GCATCGGCGA TTCGCATCTG TACTTCGTCG ATCGCTGGCG CGGGCGCAAC GGCCAGCGCG GCGGCGTGGG CGACATCTCG ATCTTCGACA TCGATTTCCG GCCGATCGAC ATCGCCACCG CGCACACCGA TCTCGATTTC GCGGGCACCG GGCTGCAGCA GGTCGACCAT TTCACGCAGA CGGTCGGCGC CGGGCGCATG CAGGAGTGGC TCGACTTCTA CCACGATCTG CTGCACTTCC GCGAGATTCA CGAAATCGAT GCGCACTGGC ACGTGTCGGA GGAGTCGCGC GTGATGGTGT CGCCGTGCGG CGCGCTGCGG ATTCCGGTGT ACGAGGAAGG CACGCGCCGC ACCGAGCTGA TGCACGCGTA TCTGCCCGAC CATCCGGGCG AGGGCGTCCA GCACATCGCG CTCGCGACGG ACGACATCCT CGCGTCGGTC GACGCGTTGC GGGCGAACGG CGTCGAGTTC ATCGAGCCGC CCGCGCGCTA CTACGACGAG GTGGACCAGC GGTTGCCGGG GCACGGCGTC GATCTGGACG CGCTGCGCCG TCGTGCGGTA TTGATCGACG GCGAGATCGG CGAAGACGGC GTGCCGCGCC TGTTCTTCCA GACGTTCGTC AAGCGCCGGC CCGGCGAAAT CTTCTTCGAG ATCGTGCAGC GCAAGGGGCA TCACGGTTTC GGCGAAGGCA ATCTCGCGGC GCTCGCGCGC GCTCGCGACG CAAGC
Details for BumuA.00134.a.B1.GE39691
HARVESTED ON: 12/3/2015
SEQUENCED ON: 12/7/2015
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS GNPHPLSSDT PPVDDPAANP LGMAGIEFVE FAAPVPDVLA QRFEQLGFKA IARHVSKAVT LYRQGQMHFL INAEPDSFAA RYAEEYGMGV CAIGIRVANA RRSFERAIEL GAWAFEGERV GVGELKIPAI QGIGDSHLYF VDRWRGRNGQ RGGVGDISIF DIDFRPIDIA TAHTDLDFAG TGLQQVDHFT QTVGAGRMQE WLDFYHDLLH FREIHEIDAH WHVSEESRVM VSPCGALRIP VYEEGTRRTE LMHAYLPDHP GEGVQHIALA TDDILASVDA LRANGVEFIE PPARYYDEVD QRLPGHGVDL DALRRRAVLI DGEIGEDGVP RLFFQTFVKR RPGEIFFEIV QRKGHHGFGE GN
Validated NT Sequence
gattgccttc gccgaaaccg tgatgcccct tgcgctgcac gatctcgaag aagatttcgc cgggccggcg cttgacgaac gtctggaaga acaggcgcgg cacgccgtct tcgccgatct cgccgtcgat caataccgca cgacggcgca gcgcgtccag atcgacgccg tgccccggca accgctggtc cacctcgtcg tagtagcgcg cgggcggctc gatgaactcg acgccgttcg cccgcaacgc gtcgaccgac gcgaggatgt cgtccgtcgc gagcgcgatg tgctggacgc cctcgcccgg atggtcgggc agatacgcgt gcatcagctc ggtgcggcgc gtgccttcct cgtacaccgg aatccgcagc gcgccgcacg gcgacaccat cacgcgcgac tcctccgaca cgtgccagtg cgcatcgatt tcgtgaatct cgcggaagtg cagcagatcg tggtagaagt cgagccactc ctgcatgcgc ccggcgccga ccgtctgcgt gaaatggtcg acctgctgca gcccggtgcc cgcgaaatcg agatcggtgt gcgcggtggc gatgtcgatc ggccggaaat cgatgtcgaa gatcgagatg tcgcccacgc cgccgcgctg gccgttgcgc ccgcgccagc gatcgacgaa gtacagatgc gaatcgccga tgccctggat cgccggaatc ttcagctcac cgacgccgac gcgctcgccc tcgaacgccc acgcgcccag ttcgatcgcg cgttcgaacg agcgccgcgc gttcgcgacg cggatgccga tcgcgcagac tcccatcccg tattcctccg catagcgcgc ggcgaacgag tccggttcag cgttgatcag gaaatgcatc tgcccctgcc gatacagcgt cacggccttg ctgacgtgcc gcgcgatcgc cttgaagccg agctgttcga aacgctgcgc gagcacgtcc ggcaccggcg ccgcgaactc gacgaactcg atccccgcca tcccgagcgg attggcggcc ggatcgtcga cgggcggcgt atcgctggac agtggatggg gattgccgga catatggtgg tggtggtggt gagccatgg
Expected Protein Sequence
MAHHHHHHMS GNPHPLSSDT PPVDDPAANP LGMAGIEFVE FAAPVPDVLA QRFEQLGFKA IARHVSKAVT LYRQGQMHFL INAEPDSFAA RYAEEYGMGV CAIGIRVANA RRSFERAIEL GAWAFEGERV GVGELKIPAI QGIGDSHLYF VDRWRGRNGQ RGGVGDISIF DIDFRPIDIA TAHTDLDFAG TGLQQVDHFT QTVGAGRMQE WLDFYHDLLH FREIHEIDAH WHVSEESRVM VSPCGALRIP VYEEGTRRTE LMHAYLPDHP GEGVQHIALA TDDILASVDA LRANGVEFIE PPARYYDEVD QRLPGHGVDL DALRRRAVLI DGEIGEDGVP RLFFQTFVKR RPGEIFFEIV QRKGHHGFGE GNLAALARAR DAS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg gcaatcccca tccactgtcc agcgatacgc cgcccgtcga cgatccggcc gccaatccgc tcgggatggc ggggatcgag ttcgtcgagt tcgcggcgcc ggtgccggac gtgctcgcgc agcgtttcga acagctcggc ttcaaggcga tcgcgcggca cgtcagcaag gccgtgacgc tgtatcggca ggggcagatg catttcctga tcaacgctga accggactcg ttcgccgcgc gctatgcgga ggaatacggg atgggagtct gcgcgatcgg catccgcgtc gcgaacgcgc ggcgctcgtt cgaacgcgcg atcgaactgg gcgcgtgggc gttcgagggc gagcgcgtcg gcgtcggtga gctgaagatt ccggcgatcc agggcatcgg cgattcgcat ctgtacttcg tcgatcgctg gcgcgggcgc aacggccagc gcggcggcgt gggcgacatc tcgatcttcg acatcgattt ccggccgatc gacatcgcca ccgcgcacac cgatctcgat ttcgcgggca ccgggctgca gcaggtcgac catttcacgc agacggtcgg cgccgggcgc atgcaggagt ggctcgactt ctaccacgat ctgctgcact tccgcgagat tcacgaaatc gatgcgcact ggcacgtgtc ggaggagtcg cgcgtgatgg tgtcgccgtg cggcgcgctg cggattccgg tgtacgagga aggcacgcgc cgcaccgagc tgatgcacgc gtatctgccc gaccatccgg gcgagggcgt ccagcacatc gcgctcgcga cggacgacat cctcgcgtcg gtcgacgcgt tgcgggcgaa cggcgtcgag ttcatcgagc cgcccgcgcg ctactacgac gaggtggacc agcggttgcc ggggcacggc gtcgatctgg acgcgctgcg ccgtcgtgcg gtattgatcg acggcgagat cggcgaagac ggcgtgccgc gcctgttctt ccagacgttc gtcaagcgcc ggcccggcga aatcttcttc gagatcgtgc agcgcaaggg gcatcacggt ttcggcgaag gcaatctcgc ggcgctcgcg cgcgctcgcg acgcaagctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BumuA.00134.a.B1.PS37893
PURIFICATION DATe: 3/31/2016
CONCENTRATION: 49.1mg/ml
OBSERVED MW: 46kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS GNPHPLSSDT PPVDDPAANP LGMAGIEFVE FAAPVPDVLA QRFEQLGFKA IARHVSKAVT LYRQGQMHFL INAEPDSFAA RYAEEYGMGV CAIGIRVANA RRSFERAIEL GAWAFEGERV GVGELKIPAI QGIGDSHLYF VDRWRGRNGQ RGGVGDISIF DIDFRPIDIA TAHTDLDFAG TGLQQVDHFT QTVGAGRMQE WLDFYHDLLH FREIHEIDAH WHVSEESRVM VSPCGALRIP VYEEGTRRTE LMHAYLPDHP GEGVQHIALA TDDILASVDA LRANGVEFIE PPARYYDEVD QRLPGHGVDL DALRRRAVLI DGEIGEDGVP RLFFQTFVKR RPGEIFFEIV QRKGHHGFGE GN
Validated NT Sequence
gattgccttc gccgaaaccg tgatgcccct tgcgctgcac gatctcgaag aagatttcgc cgggccggcg cttgacgaac gtctggaaga acaggcgcgg cacgccgtct tcgccgatct cgccgtcgat caataccgca cgacggcgca gcgcgtccag atcgacgccg tgccccggca accgctggtc cacctcgtcg tagtagcgcg cgggcggctc gatgaactcg acgccgttcg cccgcaacgc gtcgaccgac gcgaggatgt cgtccgtcgc gagcgcgatg tgctggacgc cctcgcccgg atggtcgggc agatacgcgt gcatcagctc ggtgcggcgc gtgccttcct cgtacaccgg aatccgcagc gcgccgcacg gcgacaccat cacgcgcgac tcctccgaca cgtgccagtg cgcatcgatt tcgtgaatct cgcggaagtg cagcagatcg tggtagaagt cgagccactc ctgcatgcgc ccggcgccga ccgtctgcgt gaaatggtcg acctgctgca gcccggtgcc cgcgaaatcg agatcggtgt gcgcggtggc gatgtcgatc ggccggaaat cgatgtcgaa gatcgagatg tcgcccacgc cgccgcgctg gccgttgcgc ccgcgccagc gatcgacgaa gtacagatgc gaatcgccga tgccctggat cgccggaatc ttcagctcac cgacgccgac gcgctcgccc tcgaacgccc acgcgcccag ttcgatcgcg cgttcgaacg agcgccgcgc gttcgcgacg cggatgccga tcgcgcagac tcccatcccg tattcctccg catagcgcgc ggcgaacgag tccggttcag cgttgatcag gaaatgcatc tgcccctgcc gatacagcgt cacggccttg ctgacgtgcc gcgcgatcgc cttgaagccg agctgttcga aacgctgcgc gagcacgtcc ggcaccggcg ccgcgaactc gacgaactcg atccccgcca tcccgagcgg attggcggcc ggatcgtcga cgggcggcgt atcgctggac agtggatggg gattgccgga catatggtgg tggtggtggt gagccatgg
Expressed Protein Sequence
MAHHHHHHMS GNPHPLSSDT PPVDDPAANP LGMAGIEFVE FAAPVPDVLA QRFEQLGFKA IARHVSKAVT LYRQGQMHFL INAEPDSFAA RYAEEYGMGV CAIGIRVANA RRSFERAIEL GAWAFEGERV GVGELKIPAI QGIGDSHLYF VDRWRGRNGQ RGGVGDISIF DIDFRPIDIA TAHTDLDFAG TGLQQVDHFT QTVGAGRMQE WLDFYHDLLH FREIHEIDAH WHVSEESRVM VSPCGALRIP VYEEGTRRTE LMHAYLPDHP GEGVQHIALA TDDILASVDA LRANGVEFIE PPARYYDEVD QRLPGHGVDL DALRRRAVLI DGEIGEDGVP RLFFQTFVKR RPGEIFFEIV QRKGHHGFGE GNLAALARAR DAS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG GCAATCCCCA TCCACTGTCC AGCGATACGC CGCCCGTCGA CGATCCGGCC GCCAATCCGC TCGGGATGGC GGGGATCGAG TTCGTCGAGT TCGCGGCGCC GGTGCCGGAC GTGCTCGCGC AGCGTTTCGA ACAGCTCGGC TTCAAGGCGA TCGCGCGGCA CGTCAGCAAG GCCGTGACGC TGTATCGGCA GGGGCAGATG CATTTCCTGA TCAACGCTGA ACCGGACTCG TTCGCCGCGC GCTATGCGGA GGAATACGGG ATGGGAGTCT GCGCGATCGG CATCCGCGTC GCGAACGCGC GGCGCTCGTT CGAACGCGCG ATCGAACTGG GCGCGTGGGC GTTCGAGGGC GAGCGCGTCG GCGTCGGTGA GCTGAAGATT CCGGCGATCC AGGGCATCGG CGATTCGCAT CTGTACTTCG TCGATCGCTG GCGCGGGCGC AACGGCCAGC GCGGCGGCGT GGGCGACATC TCGATCTTCG ACATCGATTT CCGGCCGATC GACATCGCCA CCGCGCACAC CGATCTCGAT TTCGCGGGCA CCGGGCTGCA GCAGGTCGAC CATTTCACGC AGACGGTCGG CGCCGGGCGC ATGCAGGAGT GGCTCGACTT CTACCACGAT CTGCTGCACT TCCGCGAGAT TCACGAAATC GATGCGCACT GGCACGTGTC GGAGGAGTCG CGCGTGATGG TGTCGCCGTG CGGCGCGCTG CGGATTCCGG TGTACGAGGA AGGCACGCGC CGCACCGAGC TGATGCACGC GTATCTGCCC GACCATCCGG GCGAGGGCGT CCAGCACATC GCGCTCGCGA CGGACGACAT CCTCGCGTCG GTCGACGCGT TGCGGGCGAA CGGCGTCGAG TTCATCGAGC CGCCCGCGCG CTACTACGAC GAGGTGGACC AGCGGTTGCC GGGGCACGGC GTCGATCTGG ACGCGCTGCG CCGTCGTGCG GTATTGATCG ACGGCGAGAT CGGCGAAGAC GGCGTGCCGC GCCTGTTCTT CCAGACGTTC GTCAAGCGCC GGCCCGGCGA AATCTTCTTC GAGATCGTGC AGCGCAAGGG GCATCACGGT TTCGGCGAAG GCAATCTCGC GGCGCTCGCG CGCGCTCGCG ACGCAAGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT