BumuA.00119.a

D-alanine--D-alanine ligase

CENTER ID: BumuA.00119.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00119.a.A1.GE34670 Full length( BumuA.00119.a ) 1 313
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00119.a.A1.PW35280 Full length( BumuA.00119.a ) 1 313

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.3065
RefSeq: YP_001581015.1
UniProt: A9AI94

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGIDPKRFG KVAVLFGGES AEREVSLTSG RLVLQGLRDA GVDAHPFDPA ERPLSALKDE GFVRAFNALH GGYGENGQIQ GALDFYGIRY TGSGVLGSAL GLDKFRTKLV WQQTGVPTPP FETVMRDDDY AARATEIVAK LGLPLFVKPA SEGSSVAVLK VKTADALPAA LAEAATHDKI VIVEKSIEGG GEYTACIAGD LDLPLIKIVP AGEFYDYHAK YVADDTQYLI PCGLPADQEA QLKRLARRAF DVLGCTDWGR ADFMLDAAGN AYFLEVNTAP GMTDHSLPPK AARAIGISYS ELVVKVLSLT LND
NT Sequence
ATGAGCGGGA TCGATCCGAA ACGTTTCGGC AAGGTGGCGG TGTTGTTCGG CGGCGAATCC GCCGAGCGCG AGGTGTCGCT CACCTCGGGC CGCCTCGTGC TGCAGGGGCT GCGCGACGCA GGCGTCGACG CCCATCCGTT CGACCCGGCC GAGCGGCCGC TGTCGGCGCT GAAGGACGAA GGCTTCGTGC GCGCGTTCAA CGCGCTGCAC GGCGGCTACG GCGAGAACGG CCAGATCCAG GGCGCGCTCG ACTTCTACGG CATTCGCTAC ACGGGCAGCG GCGTGCTCGG CTCGGCGCTC GGGCTCGACA AGTTCCGCAC GAAGCTCGTG TGGCAGCAGA CGGGCGTGCC GACGCCGCCG TTCGAGACGG TGATGCGCGA CGACGACTAC GCCGCGCGCG CGACCGAGAT CGTCGCGAAA CTCGGCCTGC CGCTGTTCGT GAAGCCGGCG AGCGAAGGCT CGAGCGTCGC GGTGCTGAAG GTGAAGACGG CCGATGCGCT GCCGGCCGCG CTCGCGGAAG CCGCGACGCA CGACAAGATC GTGATCGTCG AGAAGAGCAT CGAAGGCGGC GGCGAATACA CCGCGTGCAT CGCCGGCGAT CTCGATCTGC CGCTGATCAA GATCGTGCCG GCGGGCGAGT TCTACGACTA TCACGCGAAG TACGTGGCCG ACGACACGCA ATACCTGATT CCGTGCGGGC TGCCGGCCGA CCAGGAAGCG CAGCTGAAGC GCCTCGCGCG CCGCGCGTTC GACGTGCTCG GCTGCACCGA CTGGGGCCGG GCGGACTTCA TGCTCGACGC GGCCGGCAAC GCGTACTTCC TCGAAGTGAA CACGGCGCCT GGCATGACCG ACCACTCGCT GCCGCCGAAG GCCGCGCGCG CGATCGGCAT CAGCTACTCG GAACTGGTCG TGAAGGTGCT GTCGCTCACG CTCAACGAC
Details for BumuA.00119.a.A1.GE34670
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSGIDPKRF GKVAVLFGGE SAEREVSLTS GRLVLQGLRD AGVDAHPFDP AERPLSALKD EGFVRAFNAL HGGYGENGQI QGALDFYGIR YTGSGVLGSA LGLDKFRTKL VWQQTGVPTP PFETVMRDDD YAARATEIVA KLGLPLFVKP ASEGSSVAVL KVKTADALPA ALAEAATHDK IVIVEKSIEG GGEYTACIAG DLDLPLIKIV PAGEFYDYHA KYVADDTQYL IPCGLPADQE AQLKRLARRA FDVLGCTDWG RADFMLDAAG NAYFLEVNTA PGMTDHSLPP KAARAIGISY SELVVKVLSL TL
Validated NT Sequence
tttnnnanng agnnntacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagcgg gatcgatccg aaacgtttcg gcaaggtggc ggtgttgttc ggcggcgaat ccgccgagcg cgaggtgtcg ctcacctcgg gccgcctcgt gctgcagggg ctgcgcgacg caggcgtcga cgcccatccg ttcgacccgg ccgagcggcc gctgtcggcg ctgaaggacg aaggcttcgt gcgcgcgttc aacgcgctgc acggcggcta cggcgagaac ggccagatcc agggcgcgct cgacttctac ggcattcgct acacgggcag cggcgtgctc ggctcggcgc tcgggctcga caagttccgc acgaagctcg tgtggcagca gacgggcgtg ccgacgccgc cgttcgagac ggtgatgcgc gacgacgact acgccgcgcg cgcgaccgag atcgtcgcga aactcggcct gccgctgttc gtgaagccgg cgagcgaagg ctcgagcgtc gcggtgctga aggtgaagac ggccgatgcg ctgccggccg cgctcgcgga agccgcgacg cacgacaaga tcgtgatcgt cgagaagagc atcgaaggcg gcggcgaata caccgcgtgc atcgccggcg atctcgatct gccgctgatc aagatcgtgc cggcgggcga gttctacgac tatcacgcga agtacgtggc cgacgacacg caatacctga ttccgtgcgg gctgccggcc gaccaggaag cgcagctgaa gcgcctcgcg cgccgcgcgt tcgacgtgct cggctgcacc gactggggcc gggcggactt catgctcgac gcggccggca acgcgtactt cctcgaagtg aacacggcgc ctggcatgac cgaccactcg ctgccgccga aggccgcgcg cgcgatcggc atcagctact cggaactggt cgtgaaggtg ctgtcgctca cgctca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGIDPKRF GKVAVLFGGE SAEREVSLTS GRLVLQGLRD AGVDAHPFDP AERPLSALKD EGFVRAFNAL HGGYGENGQI QGALDFYGIR YTGSGVLGSA LGLDKFRTKL VWQQTGVPTP PFETVMRDDD YAARATEIVA KLGLPLFVKP ASEGSSVAVL KVKTADALPA ALAEAATHDK IVIVEKSIEG GGEYTACIAG DLDLPLIKIV PAGEFYDYHA KYVADDTQYL IPCGLPADQE AQLKRLARRA FDVLGCTDWG RADFMLDAAG NAYFLEVNTA PGMTDHSLPP KAARAIGISY SELVVKVLSL TLND
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgggatc gatccgaaac gtttcggcaa ggtggcggtg ttgttcggcg gcgaatccgc cgagcgcgag gtgtcgctca cctcgggccg cctcgtgctg caggggctgc gcgacgcagg cgtcgacgcc catccgttcg acccggccga gcggccgctg tcggcgctga aggacgaagg cttcgtgcgc gcgttcaacg cgctgcacgg cggctacggc gagaacggcc agatccaggg cgcgctcgac ttctacggca ttcgctacac gggcagcggc gtgctcggct cggcgctcgg gctcgacaag ttccgcacga agctcgtgtg gcagcagacg ggcgtgccga cgccgccgtt cgagacggtg atgcgcgacg acgactacgc cgcgcgcgcg accgagatcg tcgcgaaact cggcctgccg ctgttcgtga agccggcgag cgaaggctcg agcgtcgcgg tgctgaaggt gaagacggcc gatgcgctgc cggccgcgct cgcggaagcc gcgacgcacg acaagatcgt gatcgtcgag aagagcatcg aaggcggcgg cgaatacacc gcgtgcatcg ccggcgatct cgatctgccg ctgatcaaga tcgtgccggc gggcgagttc tacgactatc acgcgaagta cgtggccgac gacacgcaat acctgattcc gtgcgggctg ccggccgacc aggaagcgca gctgaagcgc ctcgcgcgcc gcgcgttcga cgtgctcggc tgcaccgact ggggccgggc ggacttcatg ctcgacgcgg ccggcaacgc gtacttcctc gaagtgaaca cggcgcctgg catgaccgac cactcgctgc cgccgaaggc cgcgcgcgcg atcggcatca gctactcgga actggtcgtg aaggtgctgt cgctcacgct caacgactaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BumuA.00119.a.A1.PW35280
PURIFICATION DATe: 4/13/2012
CONCENTRATION: 37.02mg/ml
OBSERVED MW: 33kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 17
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSGIDPKRF GKVAVLFGGE SAEREVSLTS GRLVLQGLRD AGVDAHPFDP AERPLSALKD EGFVRAFNAL HGGYGENGQI QGALDFYGIR YTGSGVLGSA LGLDKFRTKL VWQQTGVPTP PFETVMRDDD YAARATEIVA KLGLPLFVKP ASEGSSVAVL KVKTADALPA ALAEAATHDK IVIVEKSIEG GGEYTACIAG DLDLPLIKIV PAGEFYDYHA KYVADDTQYL IPCGLPADQE AQLKRLARRA FDVLGCTDWG RADFMLDAAG NAYFLEVNTA PGMTDHSLPP KAARAIGISY SELVVKVLSL TL
Validated NT Sequence
tttnnnanng agnnntacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagcgg gatcgatccg aaacgtttcg gcaaggtggc ggtgttgttc ggcggcgaat ccgccgagcg cgaggtgtcg ctcacctcgg gccgcctcgt gctgcagggg ctgcgcgacg caggcgtcga cgcccatccg ttcgacccgg ccgagcggcc gctgtcggcg ctgaaggacg aaggcttcgt gcgcgcgttc aacgcgctgc acggcggcta cggcgagaac ggccagatcc agggcgcgct cgacttctac ggcattcgct acacgggcag cggcgtgctc ggctcggcgc tcgggctcga caagttccgc acgaagctcg tgtggcagca gacgggcgtg ccgacgccgc cgttcgagac ggtgatgcgc gacgacgact acgccgcgcg cgcgaccgag atcgtcgcga aactcggcct gccgctgttc gtgaagccgg cgagcgaagg ctcgagcgtc gcggtgctga aggtgaagac ggccgatgcg ctgccggccg cgctcgcgga agccgcgacg cacgacaaga tcgtgatcgt cgagaagagc atcgaaggcg gcggcgaata caccgcgtgc atcgccggcg atctcgatct gccgctgatc aagatcgtgc cggcgggcga gttctacgac tatcacgcga agtacgtggc cgacgacacg caatacctga ttccgtgcgg gctgccggcc gaccaggaag cgcagctgaa gcgcctcgcg cgccgcgcgt tcgacgtgct cggctgcacc gactggggcc gggcggactt catgctcgac gcggccggca acgcgtactt cctcgaagtg aacacggcgc ctggcatgac cgaccactcg ctgccgccga aggccgcgcg cgcgatcggc atcagctact cggaactggt cgtgaaggtg ctgtcgctca cgctca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGIDPKRF GKVAVLFGGE SAEREVSLTS GRLVLQGLRD AGVDAHPFDP AERPLSALKD EGFVRAFNAL HGGYGENGQI QGALDFYGIR YTGSGVLGSA LGLDKFRTKL VWQQTGVPTP PFETVMRDDD YAARATEIVA KLGLPLFVKP ASEGSSVAVL KVKTADALPA ALAEAATHDK IVIVEKSIEG GGEYTACIAG DLDLPLIKIV PAGEFYDYHA KYVADDTQYL IPCGLPADQE AQLKRLARRA FDVLGCTDWG RADFMLDAAG NAYFLEVNTA PGMTDHSLPP KAARAIGISY SELVVKVLSL TLND
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGGGATC GATCCGAAAC GTTTCGGCAA GGTGGCGGTG TTGTTCGGCG GCGAATCCGC CGAGCGCGAG GTGTCGCTCA CCTCGGGCCG CCTCGTGCTG CAGGGGCTGC GCGACGCAGG CGTCGACGCC CATCCGTTCG ACCCGGCCGA GCGGCCGCTG TCGGCGCTGA AGGACGAAGG CTTCGTGCGC GCGTTCAACG CGCTGCACGG CGGCTACGGC GAGAACGGCC AGATCCAGGG CGCGCTCGAC TTCTACGGCA TTCGCTACAC GGGCAGCGGC GTGCTCGGCT CGGCGCTCGG GCTCGACAAG TTCCGCACGA AGCTCGTGTG GCAGCAGACG GGCGTGCCGA CGCCGCCGTT CGAGACGGTG ATGCGCGACG ACGACTACGC CGCGCGCGCG ACCGAGATCG TCGCGAAACT CGGCCTGCCG CTGTTCGTGA AGCCGGCGAG CGAAGGCTCG AGCGTCGCGG TGCTGAAGGT GAAGACGGCC GATGCGCTGC CGGCCGCGCT CGCGGAAGCC GCGACGCACG ACAAGATCGT GATCGTCGAG AAGAGCATCG AAGGCGGCGG CGAATACACC GCGTGCATCG CCGGCGATCT CGATCTGCCG CTGATCAAGA TCGTGCCGGC GGGCGAGTTC TACGACTATC ACGCGAAGTA CGTGGCCGAC GACACGCAAT ACCTGATTCC GTGCGGGCTG CCGGCCGACC AGGAAGCGCA GCTGAAGCGC CTCGCGCGCC GCGCGTTCGA CGTGCTCGGC TGCACCGACT GGGGCCGGGC GGACTTCATG CTCGACGCGG CCGGCAACGC GTACTTCCTC GAAGTGAACA CGGCGCCTGG CATGACCGAC CACTCGCTGC CGCCGAAGGC CGCGCGCGCG ATCGGCATCA GCTACTCGGA ACTGGTCGTG AAGGTGCTGT CGCTCACGCT CAACGACTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA