SSGCID: Target Page

BumuA.00117.c

Intradiol ring-cleavage dioxygenase

CENTER ID:	BumuA.00117.c
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00117.c.B1.GE39726	Full length( BumuA.00117.c )		1	309

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00117.c.B1.PW37878	Full length( BumuA.00117.c )		1	309

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.6396
RefSeq:	YP_001586020.1
UniProt:	A9AT56

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHGKGARRGE RRSASKETNV TNDHDDGDTR LTEQVVASFD GAPNPRVRML MQSLVRHLHA FVRETELTEA EWMAAIRFLT ETGQMCDDVV RQEFILLSDT LGVSMLVDAI NHRLATGATE TTVFGPFYIE GMPDRAYGEN MALTSGTPAL VHGRVLTTDG APLPDAVLDV WQTADNGMYS GQDPAQPHGN LRGRYRTDAQ GHYAITTILP VSYPIPTDGP VGKMLDATGR HPWRPAHLHF MIDAPGYRRL VTHLFDEEDA YLQSDAVFGV KPSLMVAYRR REAGDELARR FGISGAYREA TYDFVLDRS

NT Sequence

ATGCATGGGA AGGGCGCGCG CCGCGGTGAG CGGCGGTCCG CGTCCAAGGA GACGAACGTG ACGAATGATC ATGATGACGG CGATACGCGG TTGACCGAGC AGGTCGTCGC CAGCTTCGAC GGCGCGCCGA ACCCGCGCGT GCGCATGCTG ATGCAGAGCC TCGTGCGGCA CCTGCATGCG TTCGTCCGCG AAACCGAGCT GACCGAAGCC GAGTGGATGG CCGCGATTCG CTTCCTGACC GAAACCGGGC AGATGTGCGA CGACGTCGTG CGGCAGGAGT TCATCCTGCT GTCCGACACG CTCGGTGTGT CGATGCTGGT CGACGCGATC AACCATCGCT TGGCGACCGG CGCGACGGAA ACGACGGTGT TTGGACCGTT CTACATCGAA GGGATGCCCG ATCGCGCGTA TGGCGAGAAC ATGGCGCTCA CGTCCGGCAC GCCGGCGCTC GTGCATGGCC GCGTGCTGAC CACCGACGGT GCGCCGCTGC CGGATGCCGT GCTCGACGTC TGGCAGACCG CCGACAACGG CATGTATTCC GGACAGGACC CGGCGCAACC GCACGGCAAC CTGCGAGGCC GCTACCGGAC CGATGCGCAA GGGCACTACG CGATCACGAC GATTCTGCCG GTCAGCTATC CGATTCCGAC CGACGGCCCG GTCGGCAAGA TGCTCGACGC GACCGGGCGC CACCCGTGGC GCCCCGCGCA TCTGCACTTC ATGATCGATG CGCCCGGCTA TCGCAGGCTC GTCACGCATC TGTTCGACGA GGAAGACGCG TACCTGCAGT CGGATGCCGT GTTTGGCGTG AAGCCGTCGC TGATGGTGGC CTATCGTCGG CGCGAAGCGG GCGATGAGCT GGCGCGCCGA TTCGGCATCA GCGGTGCGTA CCGCGAAGCG ACGTACGACT TCGTGCTCGA CCGGAGC

Details for BumuA.00117.c.B1.GE39726

HARVESTED ON:	12/3/2015
SEQUENCED ON:	12/7/2015
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	95

Validated AA Sequence

MAHHHHHHMH GKGARRGERR XASKETNVTN DHDDGDTRLT EQVVASFDGA PNPRVRMLMX SLVRHLHAFV RETELTEAEW MAAIRFLTET GQMCDDVVRQ EFILLSDTLG VSMLVDAINH RLATGATETT VFGPFYXXGM PDRAYGENMX LTSGTPALVH GRVLTTDGAP LPDAVLDVWQ TADNGMYSGQ DPAQPXGNLR GRYRTDAQGX YAXXTILPVS YPIPTDGPVG KMLDATGRHP WRPAHLHFMI DAPGYRRLVT HLFDEEDAYL QSDAVFGVKP SLMVAYRRRE AGDELARRFG ISG

Validated NT Sequence

cgcaccgctg atgccgaatc ggcgcgccag ctcatcgccc gcttcgcgcc gacgataggc caccatcagc gacggcttca cgccaaacac ggcatccgac tgcaggtacg cgtcttcctc gtcgaacaga tgcgtgacga gcctgcgata gccgggcgca tcgatcatga agtgcagatg cgcggggcgc cacgggtggc gcccggtcgc gtcgagcatc ttgccgaccg ggccgtcggt cggaatcgga tagctgaccg gcagaatcgt cgnnatcgcg tagtnccctt gcgcatcggt ccggtagcgg cctcgcaggt tgccnnnngg ttgcgccggg tcctgtccgg aatacatgcc gttgtcggcg gtctgccaga cgtcgagcac ggcatccggc agcggcgcac cgtcggtggt cagcacgcgg ccatgcacga gcgccggcgt gccggacgtg agnnncatgt tctcgccata cgcgcgatcg ggcatccctn nnatgtagaa cggtccaaac accgtcgttt ccgtcgcgcc ggtcgccaag cgatggttga tcgcgtcgac cagcatcgac acaccgagcg tgtcggacag caggatgaac tcctgccgca cgacgtcgtc gcacatctgc ccggtttcgg tcaggaagcg aatcgcggcc atccactcgg cttcggtcag ctcggtttcg cggacgaacg catgcaggtg ccgcacgagg ctcngcatca gcatgcgcac gcgcgggttc ggcgcgccgt cgaagctggc gacgacctgc tcggtcaacc gcgtatcgcc gtcatcatga tcattcgtca cgttcgtctc cttggacgcg nnncgccgct caccgcggcg cgcgcccttc ccatgcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHMH GKGARRGERR SASKETNVTN DHDDGDTRLT EQVVASFDGA PNPRVRMLMQ SLVRHLHAFV RETELTEAEW MAAIRFLTET GQMCDDVVRQ EFILLSDTLG VSMLVDAINH RLATGATETT VFGPFYIEGM PDRAYGENMA LTSGTPALVH GRVLTTDGAP LPDAVLDVWQ TADNGMYSGQ DPAQPHGNLR GRYRTDAQGH YAITTILPVS YPIPTDGPVG KMLDATGRHP WRPAHLHFMI DAPGYRRLVT HLFDEEDAYL QSDAVFGVKP SLMVAYRRRE AGDELARRFG ISGAYREATY DFVLDRS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatg ggaagggcgc gcgccgcggt gagcggcggt ccgcgtccaa ggagacgaac gtgacgaatg atcatgatga cggcgatacg cggttgaccg agcaggtcgt cgccagcttc gacggcgcgc cgaacccgcg cgtgcgcatg ctgatgcaga gcctcgtgcg gcacctgcat gcgttcgtcc gcgaaaccga gctgaccgaa gccgagtgga tggccgcgat tcgcttcctg accgaaaccg ggcagatgtg cgacgacgtc gtgcggcagg agttcatcct gctgtccgac acgctcggtg tgtcgatgct ggtcgacgcg atcaaccatc gcttggcgac cggcgcgacg gaaacgacgg tgtttggacc gttctacatc gaagggatgc ccgatcgcgc gtatggcgag aacatggcgc tcacgtccgg cacgccggcg ctcgtgcatg gccgcgtgct gaccaccgac ggtgcgccgc tgccggatgc cgtgctcgac gtctggcaga ccgccgacaa cggcatgtat tccggacagg acccggcgca accgcacggc aacctgcgag gccgctaccg gaccgatgcg caagggcact acgcgatcac gacgattctg ccggtcagct atccgattcc gaccgacggc ccggtcggca agatgctcga cgcgaccggg cgccacccgt ggcgccccgc gcatctgcac ttcatgatcg atgcgcccgg ctatcgcagg ctcgtcacgc atctgttcga cgaggaagac gcgtacctgc agtcggatgc cgtgtttggc gtgaagccgt cgctgatggt ggcctatcgt cggcgcgaag cgggcgatga gctggcgcgc cgattcggca tcagcggtgc gtaccgcgaa gcgacgtacg acttcgtgct cgaccggagc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for BumuA.00117.c.B1.PW37878

PURIFICATION DATe:	3/23/2016
CONCENTRATION:	35mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCATG GGAAGGGCGC GCGCCGCGGT GAGCGGCGGT CCGCGTCCAA GGAGACGAAC GTGACGAATG ATCATGATGA CGGCGATACG CGGTTGACCG AGCAGGTCGT CGCCAGCTTC GACGGCGCGC CGAACCCGCG CGTGCGCATG CTGATGCAGA GCCTCGTGCG GCACCTGCAT GCGTTCGTCC GCGAAACCGA GCTGACCGAA GCCGAGTGGA TGGCCGCGAT TCGCTTCCTG ACCGAAACCG GGCAGATGTG CGACGACGTC GTGCGGCAGG AGTTCATCCT GCTGTCCGAC ACGCTCGGTG TGTCGATGCT GGTCGACGCG ATCAACCATC GCTTGGCGAC CGGCGCGACG GAAACGACGG TGTTTGGACC GTTCTACATC GAAGGGATGC CCGATCGCGC GTATGGCGAG AACATGGCGC TCACGTCCGG CACGCCGGCG CTCGTGCATG GCCGCGTGCT GACCACCGAC GGTGCGCCGC TGCCGGATGC CGTGCTCGAC GTCTGGCAGA CCGCCGACAA CGGCATGTAT TCCGGACAGG ACCCGGCGCA ACCGCACGGC AACCTGCGAG GCCGCTACCG GACCGATGCG CAAGGGCACT ACGCGATCAC GACGATTCTG CCGGTCAGCT ATCCGATTCC GACCGACGGC CCGGTCGGCA AGATGCTCGA CGCGACCGGG CGCCACCCGT GGCGCCCCGC GCATCTGCAC TTCATGATCG ATGCGCCCGG CTATCGCAGG CTCGTCACGC ATCTGTTCGA CGAGGAAGAC GCGTACCTGC AGTCGGATGC CGTGTTTGGC GTGAAGCCGT CGCTGATGGT GGCCTATCGT CGGCGCGAAG CGGGCGATGA GCTGGCGCGC CGATTCGGCA TCAGCGGTGC GTACCGCGAA GCGACGTACG ACTTCGTGCT CGACCGGAGC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT