BumuA.00117.a
Catechol 1,2-dioxygenase
| CENTER ID: | BumuA.00117.a |
| ORGANISM: | Burkholderia multivorans ATCC 17616 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00117.a.B1.GE39688 | Full length( BumuA.00117.a ) | 1 | 311 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00117.a.B1.PS38030 | Full length( BumuA.00117.a ) | 1 | 311 |
Structures
5UMH
DEPOSITED: 1/27/2017
DETERMINATION: XRay
CLONE: BumuA.00117.a.B1.GE39688
PROTEIN: BumuA.00117.a.B1.PS38030
9DR5
DEPOSITED: 9/25/2024
DETERMINATION: XRay
CLONE: BumuA.00117.a.B1.GE39688
PROTEIN: BumuA.00117.a.B1.PB00136
9DR6
DEPOSITED: 9/25/2024
DETERMINATION: XRay
CLONE: BumuA.00117.a.B1.GE39688
PROTEIN: BumuA.00117.a.B1.PB00136
9DR8
DEPOSITED: 9/25/2024
DETERMINATION: XRay
CLONE: BumuA.00117.a.B1.GE39688
PROTEIN: BumuA.00117.a.B1.PB00136
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_141371 |
| PATRIC ID: | fig|395019.8.peg.3490 |
| RefSeq: | YP_001583213.1 |
| UniProt: | A0A0H3KXJ8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVKVFDTKE VQDLLKAAAN LNGDAGNARF RQIVHRLLSD LFKAIDDLDI TPDEVWAGVN YLNKLGQDGE AALLAAGIGL EKYLDIRMDA ADRAAGLDGG TPRTIEGPLY VAGAPVRDGV AKIDLDDDAD AGPLVIRGTV TGTDGKPLAG ALVECWHANS KGFYSHFDPT GAQTAFNLRG AVRTDANGKY EFRTLMPVGY GCPPQGATQQ LLNGLGRHGN RPAHVHFFVS GDGHRKLTTQ FNIEGDPLIW DDFAYATREE LIPHVVDKTG GAALGMKSDA YKEIEFDIVL TPLLDGRDNQ VVHRPRASAD A
NT Sequence
ATGAGCGTCA AGGTATTCGA TACGAAGGAA GTGCAGGATC TGCTGAAGGC CGCCGCGAAC CTGAACGGCG ACGCCGGCAA CGCGCGCTTT CGCCAGATCG TGCATCGTCT GCTGAGCGAC CTGTTCAAGG CGATCGACGA TCTCGACATC ACGCCCGACG AAGTCTGGGC CGGCGTCAAC TATCTGAACA AGCTCGGCCA GGACGGCGAA GCGGCGCTGC TCGCGGCGGG CATCGGGCTC GAGAAGTATC TGGACATCCG GATGGATGCG GCCGACCGCG CGGCCGGCCT CGACGGCGGC ACGCCGCGCA CGATCGAAGG GCCGCTGTAC GTCGCAGGCG CGCCGGTGCG CGACGGCGTC GCGAAGATCG ATCTCGACGA CGATGCCGAC GCGGGCCCGC TCGTGATCCG CGGCACCGTG ACGGGCACCG ACGGCAAGCC GCTCGCCGGT GCGCTCGTCG AATGCTGGCA CGCGAATTCG AAGGGCTTCT ACTCGCACTT CGACCCGACC GGCGCGCAGA CCGCGTTCAA CCTGCGCGGC GCGGTGCGCA CCGATGCCAA CGGCAAGTAC GAATTCCGCA CGCTGATGCC GGTCGGCTAC GGCTGCCCGC CGCAAGGCGC GACGCAGCAG CTGCTGAACG GGCTCGGCCG CCACGGCAAC CGGCCGGCGC ACGTGCACTT CTTCGTGTCC GGCGACGGCC ATCGCAAGCT GACCACGCAG TTCAACATCG AAGGCGACCC GCTGATCTGG GACGACTTCG CATACGCGAC GCGCGAGGAA CTGATTCCGC ACGTGGTCGA CAAGACCGGC GGCGCCGCGC TCGGCATGAA GAGCGATGCG TACAAGGAAA TCGAGTTCGA CATCGTGCTG ACGCCGCTGC TCGACGGCCG CGACAACCAG GTCGTCCATC GTCCGCGCGC GTCCGCCGAC GCG
Details for BumuA.00117.a.B1.GE39688
| HARVESTED ON: | 12/3/2015 |
| SEQUENCED ON: | 12/7/2015 |
| EXPECTED MW: | 34kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 39 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MSVKVFDTKE VQDLLKAAAN LNGDAGNARF RQIVHRLLSD LFKAIDDLDI TPDEVWAGVN YLNKLGQDGE AGAARGGHRA REVSGHPDGC GRPRGRPRRR HAAHDRRAAV RRRRAGARRR REDRSRRRCR RGPARDPRHR DGHRRQAARR CARRMLAREF EGLLLALRPD RRADRVQPAR RGAHRCQRQV RIPHADAGRL RLPAARRDAA AAERARPPRQ PAGARALLRV RRRPSQADHA VQHRRRPADL GRLRIRDARG TDSARGRQDR RRRARHEERC VQGNRVRHRA DAAARRPRQP
Validated NT Sequence
acctggttgt cgcggccgtc gagcagcggc gtcagcacga tgtcgaactc gatttccttg tacgcatcgc tcttcatgcc gagcgcggcg ccgccggtct tgtcgaccac gtgcggaatc agttcctcgc gcgtcgcgta tgcgaagtcg tcccagatca gcgggtcgcc ttcgatgttg aactgcgtgg tcagcttgcg atggccgtcg ccggacacga agaagtgcac gtgcgccggc cggttgccgt ggcggccgag cccgttcagc agctgctgcg tcgcgccttg cggcgggcag ccgtagccga ccggcatcag cgtgcggaat tcgtacttgc cgttggcatc ggtgcgcacc gcgccgcgca ggttgaacgc ggtctgcgcg ccggtcgggt cgaagtgcga gtagaagccc ttcgaattcg cgtgccagca ttcgacgagc gcaccggcga gcggcttgcc gtcggtgccc gtcacggtgc cgcggatcac gagcgggccc gcgtcggcat cgtcgtcgag atcgatcttc gcgacgccgt cgcgcaccgg cgcgcctgcg acgtacagcg gcccttcgat cgtgcgcggc gtgccgccgt cgaggccggc cgcgcggtcg gccgcatcca tccggatgtc cagatacttc tcgagcccga tgcccgccgc gagcagcgcc cgcttcgccg tcctggccga gcttgttcag atagttgacg ccggcccaga cttcgtcggg cgtgatgtcg agatcgtcga tcgccttgaa caggtcgctc agcagacgat gcacgatctg gcgaaagcgc gcgttgccgg cgtcgccgtt caggttcgcg gcggccttca gcagatcctg cacttccttc gtatcgaata ccttgacgct catatggtgg tggtggtggt g
Expected Protein Sequence
MAHHHHHHMS VKVFDTKEVQ DLLKAAANLN GDAGNARFRQ IVHRLLSDLF KAIDDLDITP DEVWAGVNYL NKLGQDGEAA LLAAGIGLEK YLDIRMDAAD RAAGLDGGTP RTIEGPLYVA GAPVRDGVAK IDLDDDADAG PLVIRGTVTG TDGKPLAGAL VECWHANSKG FYSHFDPTGA QTAFNLRGAV RTDANGKYEF RTLMPVGYGC PPQGATQQLL NGLGRHGNRP AHVHFFVSGD GHRKLTTQFN IEGDPLIWDD FAYATREELI PHVVDKTGGA ALGMKSDAYK EIEFDIVLTP LLDGRDNQVV HRPRASADA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg tcaaggtatt cgatacgaag gaagtgcagg atctgctgaa ggccgccgcg aacctgaacg gcgacgccgg caacgcgcgc tttcgccaga tcgtgcatcg tctgctgagc gacctgttca aggcgatcga cgatctcgac atcacgcccg acgaagtctg ggccggcgtc aactatctga acaagctcgg ccaggacggc gaagcggcgc tgctcgcggc gggcatcggg ctcgagaagt atctggacat ccggatggat gcggccgacc gcgcggccgg cctcgacggc ggcacgccgc gcacgatcga agggccgctg tacgtcgcag gcgcgccggt gcgcgacggc gtcgcgaaga tcgatctcga cgacgatgcc gacgcgggcc cgctcgtgat ccgcggcacc gtgacgggca ccgacggcaa gccgctcgcc ggtgcgctcg tcgaatgctg gcacgcgaat tcgaagggct tctactcgca cttcgacccg accggcgcgc agaccgcgtt caacctgcgc ggcgcggtgc gcaccgatgc caacggcaag tacgaattcc gcacgctgat gccggtcggc tacggctgcc cgccgcaagg cgcgacgcag cagctgctga acgggctcgg ccgccacggc aaccggccgg cgcacgtgca cttcttcgtg tccggcgacg gccatcgcaa gctgaccacg cagttcaaca tcgaaggcga cccgctgatc tgggacgact tcgcatacgc gacgcgcgag gaactgattc cgcacgtggt cgacaagacc ggcggcgccg cgctcggcat gaagagcgat gcgtacaagg aaatcgagtt cgacatcgtg ctgacgccgc tgctcgacgg ccgcgacaac caggtcgtcc atcgtccgcg cgcgtccgcc gacgcgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BumuA.00117.a.B1.PS38030
| PURIFICATION DATe: | 8/24/2016 |
| CONCENTRATION: | 36mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 39 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MSVKVFDTKE VQDLLKAAAN LNGDAGNARF RQIVHRLLSD LFKAIDDLDI TPDEVWAGVN YLNKLGQDGE AGAARGGHRA REVSGHPDGC GRPRGRPRRR HAAHDRRAAV RRRRAGARRR REDRSRRRCR RGPARDPRHR DGHRRQAARR CARRMLAREF EGLLLALRPD RRADRVQPAR RGAHRCQRQV RIPHADAGRL RLPAARRDAA AAERARPPRQ PAGARALLRV RRRPSQADHA VQHRRRPADL GRLRIRDARG TDSARGRQDR RRRARHEERC VQGNRVRHRA DAAARRPRQP
Validated NT Sequence
acctggttgt cgcggccgtc gagcagcggc gtcagcacga tgtcgaactc gatttccttg tacgcatcgc tcttcatgcc gagcgcggcg ccgccggtct tgtcgaccac gtgcggaatc agttcctcgc gcgtcgcgta tgcgaagtcg tcccagatca gcgggtcgcc ttcgatgttg aactgcgtgg tcagcttgcg atggccgtcg ccggacacga agaagtgcac gtgcgccggc cggttgccgt ggcggccgag cccgttcagc agctgctgcg tcgcgccttg cggcgggcag ccgtagccga ccggcatcag cgtgcggaat tcgtacttgc cgttggcatc ggtgcgcacc gcgccgcgca ggttgaacgc ggtctgcgcg ccggtcgggt cgaagtgcga gtagaagccc ttcgaattcg cgtgccagca ttcgacgagc gcaccggcga gcggcttgcc gtcggtgccc gtcacggtgc cgcggatcac gagcgggccc gcgtcggcat cgtcgtcgag atcgatcttc gcgacgccgt cgcgcaccgg cgcgcctgcg acgtacagcg gcccttcgat cgtgcgcggc gtgccgccgt cgaggccggc cgcgcggtcg gccgcatcca tccggatgtc cagatacttc tcgagcccga tgcccgccgc gagcagcgcc cgcttcgccg tcctggccga gcttgttcag atagttgacg ccggcccaga cttcgtcggg cgtgatgtcg agatcgtcga tcgccttgaa caggtcgctc agcagacgat gcacgatctg gcgaaagcgc gcgttgccgg cgtcgccgtt caggttcgcg gcggccttca gcagatcctg cacttccttc gtatcgaata ccttgacgct catatggtgg tggtggtggt g
Expressed Protein Sequence
MAHHHHHHMS VKVFDTKEVQ DLLKAAANLN GDAGNARFRQ IVHRLLSDLF KAIDDLDITP DEVWAGVNYL NKLGQDGEAA LLAAGIGLEK YLDIRMDAAD RAAGLDGGTP RTIEGPLYVA GAPVRDGVAK IDLDDDADAG PLVIRGTVTG TDGKPLAGAL VECWHANSKG FYSHFDPTGA QTAFNLRGAV RTDANGKYEF RTLMPVGYGC PPQGATQQLL NGLGRHGNRP AHVHFFVSGD GHRKLTTQFN IEGDPLIWDD FAYATREELI PHVVDKTGGA ALGMKSDAYK EIEFDIVLTP LLDGRDNQVV HRPRASADA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG TCAAGGTATT CGATACGAAG GAAGTGCAGG ATCTGCTGAA GGCCGCCGCG AACCTGAACG GCGACGCCGG CAACGCGCGC TTTCGCCAGA TCGTGCATCG TCTGCTGAGC GACCTGTTCA AGGCGATCGA CGATCTCGAC ATCACGCCCG ACGAAGTCTG GGCCGGCGTC AACTATCTGA ACAAGCTCGG CCAGGACGGC GAAGCGGCGC TGCTCGCGGC GGGCATCGGG CTCGAGAAGT ATCTGGACAT CCGGATGGAT GCGGCCGACC GCGCGGCCGG CCTCGACGGC GGCACGCCGC GCACGATCGA AGGGCCGCTG TACGTCGCAG GCGCGCCGGT GCGCGACGGC GTCGCGAAGA TCGATCTCGA CGACGATGCC GACGCGGGCC CGCTCGTGAT CCGCGGCACC GTGACGGGCA CCGACGGCAA GCCGCTCGCC GGTGCGCTCG TCGAATGCTG GCACGCGAAT TCGAAGGGCT TCTACTCGCA CTTCGACCCG ACCGGCGCGC AGACCGCGTT CAACCTGCGC GGCGCGGTGC GCACCGATGC CAACGGCAAG TACGAATTCC GCACGCTGAT GCCGGTCGGC TACGGCTGCC CGCCGCAAGG CGCGACGCAG CAGCTGCTGA ACGGGCTCGG CCGCCACGGC AACCGGCCGG CGCACGTGCA CTTCTTCGTG TCCGGCGACG GCCATCGCAA GCTGACCACG CAGTTCAACA TCGAAGGCGA CCCGCTGATC TGGGACGACT TCGCATACGC GACGCGCGAG GAACTGATTC CGCACGTGGT CGACAAGACC GGCGGCGCCG CGCTCGGCAT GAAGAGCGAT GCGTACAAGG AAATCGAGTT CGACATCGTG CTGACGCCGC TGCTCGACGG CCGCGACAAC CAGGTCGTCC ATCGTCCGCG CGCGTCCGCC GACGCGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T