BumuA.00107.d

Efflux transporter, RND family, MFP subunit

CENTER ID:	BumuA.00107.d
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	crystal structure
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00107.d.A2.GE32157	Mature protein( BumuA.00107.d )		34	405

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00107.d.A2.PW32075	Mature protein( BumuA.00107.d )		34	405

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_155492
PATRIC ID:	fig\|395019.8.peg.3639
RefSeq:	YP_001583349.1
UniProt:	A0A0H3KTQ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAILRTSRSR IATAAVATLA VVGLGTFGAM RVNANAPEKA AAPLTEVDVA TVVPQTVTDW QSYSGRLEAV EKVDVRPQVS GTIVAVNFKD GALVKKGDVL FVIDPRPYQA EVDRAAAQLA AAQARNGYAQ SDWQRAQRLI GDNAIAKRDY DEKQNAAREA AANLKAAEAA LETARINLGY TRITAPVSGR VSRAEITLGN VVSAGASAPP LTTLVSVSPI YASFDADEQT YLQYINGARN GRKVPVELGL ANETGYSRSG EIDSVDNRLD TSSGTIRVRA RFDNADGTLV PGLYARVKVG GSAPHAALLV DDAAINTDQD KKFVFVVDQQ GRVSYREVQP GMQHGNQRVI VSGLAAGDRV IVNGTQRVRP GEQVKPHMVP MTGGDAPSAP IASTAKPAAP AKADS

NT Sequence

ATGGCCATCC TACGCACCTC CCGCTCCCGC ATCGCGACCG CTGCGGTCGC GACGCTCGCC GTCGTCGGCC TCGGGACGTT CGGCGCGATG CGCGTCAACG CGAACGCGCC CGAGAAGGCC GCCGCCCCGC TGACGGAAGT CGACGTCGCG ACGGTCGTGC CGCAGACCGT GACCGACTGG CAAAGCTATT CGGGCCGCCT CGAGGCGGTC GAGAAGGTCG ACGTGCGCCC GCAGGTATCG GGCACGATCG TCGCGGTGAA CTTCAAGGAC GGCGCGCTCG TGAAGAAGGG CGACGTGCTG TTCGTGATCG ATCCGCGCCC GTACCAGGCC GAAGTCGACC GCGCCGCCGC GCAGCTCGCG GCAGCGCAGG CACGCAACGG CTACGCGCAG AGCGACTGGC AGCGCGCGCA GCGGCTGATC GGCGACAACG CGATCGCGAA GCGCGACTAC GACGAGAAGC AGAACGCCGC GCGCGAAGCG GCCGCGAACC TCAAGGCGGC CGAAGCCGCG CTCGAGACCG CGCGCATCAA TCTCGGCTAT ACGCGGATCA CGGCGCCCGT GTCGGGCCGC GTGTCGCGCG CGGAGATCAC GCTCGGCAAC GTCGTGTCGG CCGGTGCATC GGCGCCGCCG CTCACGACGC TCGTGTCGGT GTCGCCGATC TACGCGTCGT TCGACGCGGA CGAGCAGACC TATCTGCAAT ACATCAATGG CGCGCGCAAC GGCCGCAAGG TGCCGGTCGA ACTCGGCCTC GCCAACGAAA CCGGCTATTC GCGCAGCGGC GAGATCGATT CGGTCGACAA CCGGCTCGAT ACGTCGTCCG GCACGATCCG CGTGCGTGCG CGCTTCGACA ACGCGGACGG CACGCTCGTC CCCGGCCTGT ATGCACGCGT GAAGGTGGGC GGCAGCGCGC CGCATGCGGC GCTGCTCGTC GACGATGCGG CGATCAACAC CGATCAGGAC AAGAAGTTCG TGTTCGTCGT CGATCAGCAG GGCCGCGTGT CGTATCGCGA AGTGCAGCCG GGCATGCAGC ACGGCAACCA GCGCGTGATC GTGAGCGGCC TGGCCGCCGG CGACCGCGTG ATCGTGAACG GCACGCAGCG CGTGCGCCCC GGCGAGCAGG TGAAGCCGCA CATGGTGCCG ATGACGGGCG GCGACGCGCC GTCGGCGCCG ATCGCGAGCA CGGCGAAGCC GGCCGCTCCG GCGAAGGCGG ATTCG

Details for BumuA.00107.d.A2.GE32157

HARVESTED ON:	4/26/2011
SEQUENCED ON:	5/2/2011
EXPECTED MW:	41kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	31
PERCENT COVERAGE:	30

Validated AA Sequence

MRRAAAHLHA CIQAGDERAV RVVEARTHAD RAGRRIEPVV DRIDLAAARI AGFVGEAEFD RHLAAVARAI DVLQIGLLVR VERRVDRRHR HERRERRRRC TGRHDVAERD LRARHAARHG AP

Validated NT Sequence

gcggccggct tcgccgtgct cgcgatcggc gccgacggcg cgtcgccgcc cgtcatcggc accatgtgcg gcttcacctg ctcgccgggg cgcacgcgct gcgtgccgtt cacgatcacg cggtcgccgg cggccaggcc gctcacgatc acgcgctggt tgccgtgctg catgcccggc tgcacttcgc gatacgacac gcggccctgc tgatcgacga cgaacacgaa cttcttgtcc tgatcggtgt tgatcgccgc atcgtcgacg agcagcgccg catgcggcgc gctgccgccc accttcacgc gtgcatacag gccggggacg agcgtgccgt ccgcgttgtc gaagcgcgca cgcacgcgga tcgtgccgga cgacgtatcg agccggttgt cgaccgaatc gatctcgccg ctgcgcgaat agccggtttc gttggcgagg ccgagttcga ccggcacctt gcggccgttg cgcgcgccat tgatgtattg cagataggtc tgctcgtccg cgtcgaacga cgcgtagatc ggcgacaccg acacgagcgt cgtgagcggc ggcgccgatg caccggccga cacgacgttg ccgagcgtga tctccgcgcg cgacacgcgg cccgacacgg ggcgccgtga tccgcgtata gccgagattg atgcgcgcgg tctcgagcgc ggcttcggcc gccttgaggt tcgcggccgc ttcgcgcgcg gcgttctgct tctcgtcgta gtcgcgcttc gcgatcgcgt tgtcgccgat cagccgctgc gcgcgctgcc agtcgctctg cgcgtagccg ttgcgtgcct gcgctgccgc gagctgcgcg gcggcgcggt cgacttcggc ctggtacggg cgcggatcga tcacgaacag cacgtcgccc ttcttcacga gcgcgccgtc cttgaagttc accgcgacga tcgtgcccga tacctgcggg cgcacgtcga ccttctcgac cgcctcgagg cggcccgaat agctttgcca gtcggtcacg gtctgcggca cgaccgtcg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMANAPEKAA APLTEVDVAT VVPQTVTDWQ SYSGRLEAVE KVDVRPQVSG TIVAVNFKDG ALVKKGDVLF VIDPRPYQAE VDRAAAQLAA AQARNGYAQS DWQRAQRLIG DNAIAKRDYD EKQNAAREAA ANLKAAEAAL ETARINLGYT RITAPVSGRV SRAEITLGNV VSAGASAPPL TTLVSVSPIY ASFDADEQTY LQYINGARNG RKVPVELGLA NETGYSRSGE IDSVDNRLDT SSGTIRVRAR FDNADGTLVP GLYARVKVGG SAPHAALLVD DAAINTDQDK KFVFVVDQQG RVSYREVQPG MQHGNQRVIV SGLAAGDRVI VNGTQRVRPG EQVKPHMVPM TGGDAPSAPI ASTAKPAAPA KADS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgaacgcg cccgagaagg ccgccgcccc gctgacggaa gtcgacgtcg cgacggtcgt gccgcagacc gtgaccgact ggcaaagcta ttcgggccgc ctcgaggcgg tcgagaaggt cgacgtgcgc ccgcaggtat cgggcacgat cgtcgcggtg aacttcaagg acggcgcgct cgtgaagaag ggcgacgtgc tgttcgtgat cgatccgcgc ccgtaccagg ccgaagtcga ccgcgccgcc gcgcagctcg cggcagcgca ggcacgcaac ggctacgcgc agagcgactg gcagcgcgcg cagcggctga tcggcgacaa cgcgatcgcg aagcgcgact acgacgagaa gcagaacgcc gcgcgcgaag cggccgcgaa cctcaaggcg gccgaagccg cgctcgagac cgcgcgcatc aatctcggct atacgcggat cacggcgccc gtgtcgggcc gcgtgtcgcg cgcggagatc acgctcggca acgtcgtgtc ggccggtgca tcggcgccgc cgctcacgac gctcgtgtcg gtgtcgccga tctacgcgtc gttcgacgcg gacgagcaga cctatctgca atacatcaat ggcgcgcgca acggccgcaa ggtgccggtc gaactcggcc tcgccaacga aaccggctat tcgcgcagcg gcgagatcga ttcggtcgac aaccggctcg atacgtcgtc cggcacgatc cgcgtgcgtg cgcgcttcga caacgcggac ggcacgctcg tccccggcct gtatgcacgc gtgaaggtgg gcggcagcgc gccgcatgcg gcgctgctcg tcgacgatgc ggcgatcaac accgatcagg acaagaagtt cgtgttcgtc gtcgatcagc agggccgcgt gtcgtatcgc gaagtgcagc cgggcatgca gcacggcaac cagcgcgtga tcgtgagcgg cctggccgcc ggcgaccgcg tgatcgtgaa cggcacgcag cgcgtgcgcc ccggcgagca ggtgaagccg cacatggtgc cgatgacggg cggcgacgcg ccgtcggcgc cgatcgcgag cacggcgaag ccggccgctc cggcgaaggc ggattcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for BumuA.00107.d.A2.PW32075

PURIFICATION DATe:	6/17/2011
CONCENTRATION:	21.71mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	31
PERCENT COVERAGE:	30

Protocol Notes

notes unavailable

Validated AA Sequence

MRRAAAHLHA CIQAGDERAV RVVEARTHAD RAGRRIEPVV DRIDLAAARI AGFVGEAEFD RHLAAVARAI DVLQIGLLVR VERRVDRRHR HERRERRRRC TGRHDVAERD LRARHAARHG AP

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGAACGCG CCCGAGAAGG CCGCCGCCCC GCTGACGGAA GTCGACGTCG CGACGGTCGT GCCGCAGACC GTGACCGACT GGCAAAGCTA TTCGGGCCGC CTCGAGGCGG TCGAGAAGGT CGACGTGCGC CCGCAGGTAT CGGGCACGAT CGTCGCGGTG AACTTCAAGG ACGGCGCGCT CGTGAAGAAG GGCGACGTGC TGTTCGTGAT CGATCCGCGC CCGTACCAGG CCGAAGTCGA CCGCGCCGCC GCGCAGCTCG CGGCAGCGCA GGCACGCAAC GGCTACGCGC AGAGCGACTG GCAGCGCGCG CAGCGGCTGA TCGGCGACAA CGCGATCGCG AAGCGCGACT ACGACGAGAA GCAGAACGCC GCGCGCGAAG CGGCCGCGAA CCTCAAGGCG GCCGAAGCCG CGCTCGAGAC CGCGCGCATC AATCTCGGCT ATACGCGGAT CACGGCGCCC GTGTCGGGCC GCGTGTCGCG CGCGGAGATC ACGCTCGGCA ACGTCGTGTC GGCCGGTGCA TCGGCGCCGC CGCTCACGAC GCTCGTGTCG GTGTCGCCGA TCTACGCGTC GTTCGACGCG GACGAGCAGA CCTATCTGCA ATACATCAAT GGCGCGCGCA ACGGCCGCAA GGTGCCGGTC GAACTCGGCC TCGCCAACGA AACCGGCTAT TCGCGCAGCG GCGAGATCGA TTCGGTCGAC AACCGGCTCG ATACGTCGTC CGGCACGATC CGCGTGCGTG CGCGCTTCGA CAACGCGGAC GGCACGCTCG TCCCCGGCCT GTATGCACGC GTGAAGGTGG GCGGCAGCGC GCCGCATGCG GCGCTGCTCG TCGACGATGC GGCGATCAAC ACCGATCAGG ACAAGAAGTT CGTGTTCGTC GTCGATCAGC AGGGCCGCGT GTCGTATCGC GAAGTGCAGC CGGGCATGCA GCACGGCAAC CAGCGCGTGA TCGTGAGCGG CCTGGCCGCC GGCGACCGCG TGATCGTGAA CGGCACGCAG CGCGTGCGCC CCGGCGAGCA GGTGAAGCCG CACATGGTGC CGATGACGGG CGGCGACGCG CCGTCGGCGC CGATCGCGAG CACGGCGAAG CCGGCCGCTC CGGCGAAGGC GGATTCGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA