BumuA.00107.c

Efflux transporter, RND family, MFP subunit

CENTER ID: BumuA.00107.c
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00107.c.A2.GE34681 Mature protein( BumuA.00107.c ) 26 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00107.c.A2.PW35291 Mature protein( BumuA.00107.c ) 26 386

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.4679
RefSeq: YP_001584328.1
UniProt: A0A0H3KLA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKNQKRCCHG AVCALVAAML AGCGPAERPV AAPPTPVAAM TLTASRLDVT EDLPGRVAAV RIAEIRPQVS GIVQRRLFEQ GTEVRTGQPL FQINPAPFKA EVDTAAAALQ RAQAALERAK VQTARFKPLV EADAISRQVY DDAVSQRDQA AADVAQARAT LARRQLDLKF ATVEAPIAGR IDQALVTEGA LVSSSDSQPM ARIQQIDQVY VDVRQPAASL EALRGALAAQ PQASDANGVP VDVLRADDSR YDVKGRMLFS GVNVDPGTGD VLVRVLVDNP RRELLPGMYV RARIPRAHYD HALTVPQQAI VRAGGKPQVW VLDAKGTAHL KAVEVGELSN RNYRIKSGLQ AGQKVVVEGM ERLAEGASVA ASPWQAPTAV TTASSH
NT Sequence
ATGAAGAATC AGAAACGATG CTGCCACGGC GCCGTGTGCG CGCTGGTGGC GGCGATGCTG GCGGGTTGCG GGCCCGCCGA ACGGCCGGTT GCCGCACCGC CCACGCCGGT CGCCGCGATG ACGCTGACTG CCTCGCGCCT CGACGTGACC GAAGACTTGC CGGGCCGCGT CGCGGCCGTG CGGATCGCCG AGATTCGGCC GCAGGTGAGC GGCATCGTAC AGCGCCGGCT GTTCGAGCAA GGCACCGAGG TCCGCACGGG CCAGCCGCTG TTCCAGATCA ACCCCGCGCC GTTCAAGGCC GAAGTCGACA CGGCCGCCGC GGCGCTGCAG CGTGCGCAGG CGGCGCTCGA ACGCGCGAAG GTGCAGACCG CGCGCTTCAA GCCGCTCGTC GAAGCCGATG CGATCAGCCG CCAGGTGTAC GACGACGCCG TGTCGCAACG CGACCAGGCC GCGGCCGACG TCGCGCAGGC GCGCGCGACG CTCGCGCGCC GCCAGCTCGA CCTGAAGTTC GCGACCGTCG AAGCGCCGAT CGCGGGCCGC ATCGATCAGG CGCTCGTGAC CGAAGGCGCG CTGGTATCGA GCAGCGACAG CCAGCCGATG GCGCGCATCC AGCAGATCGA CCAGGTCTAC GTGGACGTGC GCCAGCCGGC CGCATCGCTC GAAGCGCTGC GCGGCGCGCT CGCGGCGCAG CCGCAAGCGT CGGACGCCAA TGGCGTGCCG GTCGACGTGC TGCGCGCCGA CGACTCGCGC TACGACGTGA AGGGCCGCAT GCTGTTCTCG GGCGTCAACG TCGACCCCGG CACCGGCGAC GTGCTCGTGC GCGTGCTGGT GGACAACCCG AGGCGCGAGC TTCTGCCGGG CATGTACGTG CGTGCCCGCA TCCCGCGCGC GCACTACGAC CACGCGCTGA CCGTGCCGCA GCAGGCAATC GTCCGCGCCG GCGGCAAGCC GCAGGTGTGG GTGCTCGACG CCAAGGGCAC GGCCCACCTG AAGGCCGTCG AAGTCGGCGA GCTGTCGAAC CGCAACTACC GTATCAAGTC GGGACTGCAG GCCGGACAGA AGGTCGTGGT CGAAGGCATG GAGCGCCTCG CCGAAGGCGC GTCCGTCGCG GCGAGCCCAT GGCAGGCGCC CACGGCGGTC ACGACCGCCT CCTCGCAC
Details for BumuA.00107.c.A2.GE34681
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAERPVAAP PTPVAAMTLT ASRLDVTEDL PGRVAAVRIA EIRPQVSGIV QRRLFEQGTE VRTGQPLFQI NPAPFKAEVD TAAAALQRAQ AALERAKVQT ARFKPLVEAD AISRQVYDDA VSQRDQAAAD VAQARATLAR RQLDLKFATV EAPIAGRIDQ ALVTEGALVS SSDSQPMARI QQIDQVYVDV RQPAASLEAL RGALAAQPQA SDANGVPVDV LRADDSRYDV KGRMLFSGVN VDPGTGDVLV RVLVDNPRRE LLPGMYVRAR IPRAHYDHAL TVPQQAIVRA GGKPQVWVLD AKGTAHLKAV EVGELSNRNY RIKSGLQAGQ KVVVEGMERL AEGASVAASP WQAPTAVTTA SSH
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggccga acggccggtt gccgcaccgc ccacgccggt cgccgcgatg acgctgactg cctcgcgcct cgacgtgacc gaagacttgc cgggccgcgt cgcggccgtg cggatcgccg agattcggcc gcaggtgagc ggcatcgtac agcgccggct gttcgagcaa ggcaccgagg tccgcacggg ccagccgctg ttccagatca accccgcgcc gttcaaggcc gaagtcgaca cggccgccgc ggcgctgcag cgtgcgcagg cggcgctcga acgcgcgaag gtgcagaccg cgcgcttcaa gccgctcgtc gaagccgatg cgatcagccg ccaggtgtac gacgacgccg tgtcgcaacg cgaccaggcc gcggccgacg tcgcgcaggc gcgcgcgacg ctcgcgcgcc gccagctcga cctgaagttc gcgaccgtcg aagcgccgat cgcgggccgc atcgatcagg cgctcgtgac cgaaggcgcg ctggtatcga gcagcgacag ccagccgatg gcgcgcatcc agcagatcga ccaggtctac gtggacgtgc gccagccggc cgcatcgctc gaagcgctgc gcggcgcgct cgcggcgcag ccgcaagcgt cggacgccaa tggcgtgccg gtcgacgtgc tgcgcgccga cgactcgcgc tacgacgtga agggccgcat gctgttctcg ggcgtcaacg tcgaccccgg caccggcgac gtgctcgtgc gcgtgctggt ggacaacccg aggcgcgagc ttctgccggg catgtacgtg cgtgcccgca tcccgcgcgc gcactacgac cacgcgctga ccgtgccgca gcaggcaatc gtccgcgccg gcggcaagcc gcaggtgtgg gtgctcgacg ccaagggcac ggcccacctg aaggccgtcg aagtcggcga gctgtcgaac cgcaactacc gtatcaagtc gggactgcag gccggacaga aggtcgtggt cgaaggcatg gagcgcctcg ccgaaggcgc gtccgtcgcg gcgagcccat ggcaggcgcc cacggcggtc acgaccgcct cctcgcacta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAERPVAAP PTPVAAMTLT ASRLDVTEDL PGRVAAVRIA EIRPQVSGIV QRRLFEQGTE VRTGQPLFQI NPAPFKAEVD TAAAALQRAQ AALERAKVQT ARFKPLVEAD AISRQVYDDA VSQRDQAAAD VAQARATLAR RQLDLKFATV EAPIAGRIDQ ALVTEGALVS SSDSQPMARI QQIDQVYVDV RQPAASLEAL RGALAAQPQA SDANGVPVDV LRADDSRYDV KGRMLFSGVN VDPGTGDVLV RVLVDNPRRE LLPGMYVRAR IPRAHYDHAL TVPQQAIVRA GGKPQVWVLD AKGTAHLKAV EVGELSNRNY RIKSGLQAGQ KVVVEGMERL AEGASVAASP WQAPTAVTTA SSH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgaacgg ccggttgccg caccgcccac gccggtcgcc gcgatgacgc tgactgcctc gcgcctcgac gtgaccgaag acttgccggg ccgcgtcgcg gccgtgcgga tcgccgagat tcggccgcag gtgagcggca tcgtacagcg ccggctgttc gagcaaggca ccgaggtccg cacgggccag ccgctgttcc agatcaaccc cgcgccgttc aaggccgaag tcgacacggc cgccgcggcg ctgcagcgtg cgcaggcggc gctcgaacgc gcgaaggtgc agaccgcgcg cttcaagccg ctcgtcgaag ccgatgcgat cagccgccag gtgtacgacg acgccgtgtc gcaacgcgac caggccgcgg ccgacgtcgc gcaggcgcgc gcgacgctcg cgcgccgcca gctcgacctg aagttcgcga ccgtcgaagc gccgatcgcg ggccgcatcg atcaggcgct cgtgaccgaa ggcgcgctgg tatcgagcag cgacagccag ccgatggcgc gcatccagca gatcgaccag gtctacgtgg acgtgcgcca gccggccgca tcgctcgaag cgctgcgcgg cgcgctcgcg gcgcagccgc aagcgtcgga cgccaatggc gtgccggtcg acgtgctgcg cgccgacgac tcgcgctacg acgtgaaggg ccgcatgctg ttctcgggcg tcaacgtcga ccccggcacc ggcgacgtgc tcgtgcgcgt gctggtggac aacccgaggc gcgagcttct gccgggcatg tacgtgcgtg cccgcatccc gcgcgcgcac tacgaccacg cgctgaccgt gccgcagcag gcaatcgtcc gcgccggcgg caagccgcag gtgtgggtgc tcgacgccaa gggcacggcc cacctgaagg ccgtcgaagt cggcgagctg tcgaaccgca actaccgtat caagtcggga ctgcaggccg gacagaaggt cgtggtcgaa ggcatggagc gcctcgccga aggcgcgtcc gtcgcggcga gcccatggca ggcgcccacg gcggtcacga ccgcctcctc gcactaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BumuA.00107.c.A2.PW35291
PURIFICATION DATe: 4/13/2012
CONCENTRATION: 18.57mg/ml
OBSERVED MW: 42kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 25
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAERPVAAP PTPVAAMTLT ASRLDVTEDL PGRVAAVRIA EIRPQVSGIV QRRLFEQGTE VRTGQPLFQI NPAPFKAEVD TAAAALQRAQ AALERAKVQT ARFKPLVEAD AISRQVYDDA VSQRDQAAAD VAQARATLAR RQLDLKFATV EAPIAGRIDQ ALVTEGALVS SSDSQPMARI QQIDQVYVDV RQPAASLEAL RGALAAQPQA SDANGVPVDV LRADDSRYDV KGRMLFSGVN VDPGTGDVLV RVLVDNPRRE LLPGMYVRAR IPRAHYDHAL TVPQQAIVRA GGKPQVWVLD AKGTAHLKAV EVGELSNRNY RIKSGLQAGQ KVVVEGMERL AEGASVAASP WQAPTAVTTA SSH
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggccga acggccggtt gccgcaccgc ccacgccggt cgccgcgatg acgctgactg cctcgcgcct cgacgtgacc gaagacttgc cgggccgcgt cgcggccgtg cggatcgccg agattcggcc gcaggtgagc ggcatcgtac agcgccggct gttcgagcaa ggcaccgagg tccgcacggg ccagccgctg ttccagatca accccgcgcc gttcaaggcc gaagtcgaca cggccgccgc ggcgctgcag cgtgcgcagg cggcgctcga acgcgcgaag gtgcagaccg cgcgcttcaa gccgctcgtc gaagccgatg cgatcagccg ccaggtgtac gacgacgccg tgtcgcaacg cgaccaggcc gcggccgacg tcgcgcaggc gcgcgcgacg ctcgcgcgcc gccagctcga cctgaagttc gcgaccgtcg aagcgccgat cgcgggccgc atcgatcagg cgctcgtgac cgaaggcgcg ctggtatcga gcagcgacag ccagccgatg gcgcgcatcc agcagatcga ccaggtctac gtggacgtgc gccagccggc cgcatcgctc gaagcgctgc gcggcgcgct cgcggcgcag ccgcaagcgt cggacgccaa tggcgtgccg gtcgacgtgc tgcgcgccga cgactcgcgc tacgacgtga agggccgcat gctgttctcg ggcgtcaacg tcgaccccgg caccggcgac gtgctcgtgc gcgtgctggt ggacaacccg aggcgcgagc ttctgccggg catgtacgtg cgtgcccgca tcccgcgcgc gcactacgac cacgcgctga ccgtgccgca gcaggcaatc gtccgcgccg gcggcaagcc gcaggtgtgg gtgctcgacg ccaagggcac ggcccacctg aaggccgtcg aagtcggcga gctgtcgaac cgcaactacc gtatcaagtc gggactgcag gccggacaga aggtcgtggt cgaaggcatg gagcgcctcg ccgaaggcgc gtccgtcgcg gcgagcccat ggcaggcgcc cacggcggtc acgaccgcct cctcgcacta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAERPVAAP PTPVAAMTLT ASRLDVTEDL PGRVAAVRIA EIRPQVSGIV QRRLFEQGTE VRTGQPLFQI NPAPFKAEVD TAAAALQRAQ AALERAKVQT ARFKPLVEAD AISRQVYDDA VSQRDQAAAD VAQARATLAR RQLDLKFATV EAPIAGRIDQ ALVTEGALVS SSDSQPMARI QQIDQVYVDV RQPAASLEAL RGALAAQPQA SDANGVPVDV LRADDSRYDV KGRMLFSGVN VDPGTGDVLV RVLVDNPRRE LLPGMYVRAR IPRAHYDHAL TVPQQAIVRA GGKPQVWVLD AKGTAHLKAV EVGELSNRNY RIKSGLQAGQ KVVVEGMERL AEGASVAASP WQAPTAVTTA SSH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGAACGG CCGGTTGCCG CACCGCCCAC GCCGGTCGCC GCGATGACGC TGACTGCCTC GCGCCTCGAC GTGACCGAAG ACTTGCCGGG CCGCGTCGCG GCCGTGCGGA TCGCCGAGAT TCGGCCGCAG GTGAGCGGCA TCGTACAGCG CCGGCTGTTC GAGCAAGGCA CCGAGGTCCG CACGGGCCAG CCGCTGTTCC AGATCAACCC CGCGCCGTTC AAGGCCGAAG TCGACACGGC CGCCGCGGCG CTGCAGCGTG CGCAGGCGGC GCTCGAACGC GCGAAGGTGC AGACCGCGCG CTTCAAGCCG CTCGTCGAAG CCGATGCGAT CAGCCGCCAG GTGTACGACG ACGCCGTGTC GCAACGCGAC CAGGCCGCGG CCGACGTCGC GCAGGCGCGC GCGACGCTCG CGCGCCGCCA GCTCGACCTG AAGTTCGCGA CCGTCGAAGC GCCGATCGCG GGCCGCATCG ATCAGGCGCT CGTGACCGAA GGCGCGCTGG TATCGAGCAG CGACAGCCAG CCGATGGCGC GCATCCAGCA GATCGACCAG GTCTACGTGG ACGTGCGCCA GCCGGCCGCA TCGCTCGAAG CGCTGCGCGG CGCGCTCGCG GCGCAGCCGC AAGCGTCGGA CGCCAATGGC GTGCCGGTCG ACGTGCTGCG CGCCGACGAC TCGCGCTACG ACGTGAAGGG CCGCATGCTG TTCTCGGGCG TCAACGTCGA CCCCGGCACC GGCGACGTGC TCGTGCGCGT GCTGGTGGAC AACCCGAGGC GCGAGCTTCT GCCGGGCATG TACGTGCGTG CCCGCATCCC GCGCGCGCAC TACGACCACG CGCTGACCGT GCCGCAGCAG GCAATCGTCC GCGCCGGCGG CAAGCCGCAG GTGTGGGTGC TCGACGCCAA GGGCACGGCC CACCTGAAGG CCGTCGAAGT CGGCGAGCTG TCGAACCGCA ACTACCGTAT CAAGTCGGGA CTGCAGGCCG GACAGAAGGT CGTGGTCGAA GGCATGGAGC GCCTCGCCGA AGGCGCGTCC GTCGCGGCGA GCCCATGGCA GGCGCCCACG GCGGTCACGA CCGCCTCCTC GCACTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA