BumuA.00105.a

Beta-lactamase

CENTER ID: BumuA.00105.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00105.a.B1.GE39684 Full length( BumuA.00105.a ) 1 396
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_132138
PATRIC ID: fig|395019.8.peg.855
RefSeq: YP_001578900.1
UniProt: A0A0H3KQX7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSLPVIARD SEADVALDVR LDGALERALA DERIVGAVVM VARDGELRYT RAAGLADREA RTPMREDTLF RLASVTKPIV TAAAMRLVAA GRIALDEPVA RWLPEFRPTL RDGTPARITL RHLLSHTAGL GYRFLEPDDD GPYARAGVSD GMDRAPVSLA ENVRRIASVP LQFAPGTSWC YSLATDVVGA LIEAVHERPL AEAVAELVTT PLGMTATAFV APDAARVATP YVSTAGAPRR MAAVEVVPPR DGTVGIRFEP ARAFDRDAWP SGGAGMIGCA ADCVALLDAL RTGRDGWLAP KWVDQMARIQ PGAHDVPDAP GFGFGLGFSV LRDPVAAQSP ESVGTWRWGG AYGHAWFVDR AAGLTVVALT NTLYEGAHGR FVTDVRDAVY GAGRAA
NT Sequence
ATGTCATCCC TGCCTGTGAT TGCCCGCGAC AGCGAGGCCG ACGTCGCGCT GGACGTGCGG CTTGACGGCG CGCTCGAGCG CGCGCTCGCG GACGAACGGA TCGTCGGCGC GGTCGTGATG GTCGCGCGCG ACGGCGAGTT GCGCTACACG CGCGCGGCGG GCCTTGCCGA CCGCGAGGCC CGCACGCCGA TGCGCGAGGA CACGCTGTTC CGGCTCGCAT CGGTCACGAA GCCGATCGTG ACGGCCGCCG CGATGCGGCT CGTCGCGGCC GGCCGCATCG CGCTCGACGA ACCGGTCGCG CGCTGGCTGC CCGAGTTCCG GCCGACGCTG CGCGACGGCA CGCCGGCCCG CATCACGTTG CGGCATCTGC TGTCGCATAC GGCGGGCCTC GGCTATCGCT TTCTCGAACC CGACGACGAC GGTCCGTATG CGCGAGCCGG CGTATCCGAC GGCATGGATC GCGCGCCCGT GTCGCTCGCG GAGAACGTGC GGCGTATCGC GAGCGTGCCG CTGCAATTCG CGCCCGGTAC GTCGTGGTGC TATTCGCTCG CGACGGACGT GGTCGGTGCG CTGATCGAGG CCGTCCACGA AAGGCCGCTC GCCGAGGCGG TCGCCGAACT CGTCACGACA CCGCTCGGCA TGACCGCGAC CGCGTTCGTC GCGCCCGATG CCGCGCGCGT CGCGACGCCG TACGTGAGTA CGGCAGGCGC GCCGCGCCGG ATGGCGGCCG TCGAGGTCGT ACCGCCGCGC GACGGCACGG TCGGCATCCG CTTCGAACCG GCGCGTGCGT TCGATCGCGA CGCATGGCCG TCGGGCGGCG CCGGCATGAT CGGCTGCGCG GCGGACTGCG TCGCACTGCT CGACGCGCTG CGCACCGGCC GCGACGGCTG GCTCGCGCCC AAGTGGGTCG ACCAGATGGC GCGCATCCAG CCGGGCGCGC ACGACGTGCC CGACGCGCCG GGCTTCGGCT TCGGGCTCGG CTTTTCGGTG CTGCGCGATC CCGTCGCCGC GCAGTCGCCG GAGTCGGTCG GCACGTGGCG CTGGGGCGGC GCGTACGGGC ATGCGTGGTT CGTCGATCGC GCGGCCGGAC TCACGGTCGT CGCGCTGACG AACACGCTGT ACGAGGGCGC GCACGGCCGA TTCGTCACCG ACGTGCGCGA TGCGGTCTAC GGCGCGGGGA GGGCGGCA
Details for BumuA.00105.a.B1.GE39684
HARVESTED ON: data unavailable
SEQUENCED ON: 12/7/2015
EXPECTED MW: 43kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: None
TOTAL EXPRESSION LEVEL: Data Unavailable
SOLUBLE EXPRESSION LEVEL Data Unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 69
PERCENT COVERAGE: 84
Validated AA Sequence
MAHHHHHHMS SLPVIARDSE ADVALDVRLD GALERALADE RIVGAVVMVA RDGELRYTRA AGLADREART PMREDTLFRL ASVTKPIVTA AAMRLVAAGR IALDEPVARW LPEFRPTLRD GTPARITLRH LLSHTAGLGY RFLEPDDDGP YARAGVSDGM DRAPVSLAEN VRRIASVPLQ XAPGXSWCYS LATDVVGALI EAVHERPLAE AVAXXXXXXA RXXRDRVRRA RCRARRXAXX XXXXRAAXXX GRXXRXAAXX XGRXXLXXGA CVXXXXXAVG RRRXXXLRGX XRRXAXXAXX XPXXLARXXX GXXXGAXXXG RXXXAXXAGL RLRARLFGAA
Validated NT Sequence
gcgcagcacc gaaaagccga gcccgaagcc gaagcccggc gcnnngggca cnnnnnnnnc gcccgnnnnn nnncgcgccn nnnnnnngac ccannnnnnc gcgagccagc cnnngcggcc nnnnnncagc gcnnnnagca nnncgacgca nnncgccgcg cagcnnnnnn nnncggcgcc gcccgacggc cnnncnnngc gnnngaacgc acgcgccgnn nnnaagcnnn nnncgaccnn nccnnngcgc ggcgnnncga cnnngacggc cgcnnnnnng cgcggcgcgn nnnnnnnnnn nacnnncggc nnngcgacgc gcgcggcatc gggcgcgacg aacgcggtcg cggtnnnncc gagcnnnnnn nnnacnnnnn nnggcgaccg cctcggcgag cggcctttcg tggacggcct cgatcagcgc accgaccacg tccgtcgcga gcgaatagca ccacgannnn ccgggcgcga nttgcagcgg cacgctcgcg atacgccgca cgttctccgc gagcgacacg ggcgcgcgat ccatgccgtc ggatacgccg gctcgcgcat acggaccgtc gtcgtcgggt tcgagaaagc gatagccgag gcccgccgta tgcgacagca gatgccgcaa cgtgatgcgg gccggcgtgc cgtcgcgcag cgtcggccgg aactcgggca gccagcgcgc gaccggttcg tcgagcgcga tgcggccggc cgcgacgagc cgcatcgcgg cggccgtcac gatcggcttc gtgaccgatg cgagccggaa cagcgtgtcc tcgcgcatcg gcgtgcgggc ctcgcggtcg gcaaggcccg ccgcgcgcgt gtagcgcaac tcgccgtcgc gcgcgaccat cacgaccgcg ccgacgatcc gttcgtccgc gagcgcgcgc tcgagcgcgc cgtcaagccg cacgtccagc gcgacgtcgg cctcgctgtc gcgggcaatc acaggcaggg atgacatatg gtggtggtgg tggtgagcca tg
Expected Protein Sequence
MAHHHHHHMS SLPVIARDSE ADVALDVRLD GALERALADE RIVGAVVMVA RDGELRYTRA AGLADREART PMREDTLFRL ASVTKPIVTA AAMRLVAAGR IALDEPVARW LPEFRPTLRD GTPARITLRH LLSHTAGLGY RFLEPDDDGP YARAGVSDGM DRAPVSLAEN VRRIASVPLQ FAPGTSWCYS LATDVVGALI EAVHERPLAE AVAELVTTPL GMTATAFVAP DAARVATPYV STAGAPRRMA AVEVVPPRDG TVGIRFEPAR AFDRDAWPSG GAGMIGCAAD CVALLDALRT GRDGWLAPKW VDQMARIQPG AHDVPDAPGF GFGLGFSVLR DPVAAQSPES VGTWRWGGAY GHAWFVDRAA GLTVVALTNT LYEGAHGRFV TDVRDAVYGA GRAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcat ccctgcctgt gattgcccgc gacagcgagg ccgacgtcgc gctggacgtg cggcttgacg gcgcgctcga gcgcgcgctc gcggacgaac ggatcgtcgg cgcggtcgtg atggtcgcgc gcgacggcga gttgcgctac acgcgcgcgg cgggccttgc cgaccgcgag gcccgcacgc cgatgcgcga ggacacgctg ttccggctcg catcggtcac gaagccgatc gtgacggccg ccgcgatgcg gctcgtcgcg gccggccgca tcgcgctcga cgaaccggtc gcgcgctggc tgcccgagtt ccggccgacg ctgcgcgacg gcacgccggc ccgcatcacg ttgcggcatc tgctgtcgca tacggcgggc ctcggctatc gctttctcga acccgacgac gacggtccgt atgcgcgagc cggcgtatcc gacggcatgg atcgcgcgcc cgtgtcgctc gcggagaacg tgcggcgtat cgcgagcgtg ccgctgcaat tcgcgcccgg tacgtcgtgg tgctattcgc tcgcgacgga cgtggtcggt gcgctgatcg aggccgtcca cgaaaggccg ctcgccgagg cggtcgccga actcgtcacg acaccgctcg gcatgaccgc gaccgcgttc gtcgcgcccg atgccgcgcg cgtcgcgacg ccgtacgtga gtacggcagg cgcgccgcgc cggatggcgg ccgtcgaggt cgtaccgccg cgcgacggca cggtcggcat ccgcttcgaa ccggcgcgtg cgttcgatcg cgacgcatgg ccgtcgggcg gcgccggcat gatcggctgc gcggcggact gcgtcgcact gctcgacgcg ctgcgcaccg gccgcgacgg ctggctcgcg cccaagtggg tcgaccagat ggcgcgcatc cagccgggcg cgcacgacgt gcccgacgcg ccgggcttcg gcttcgggct cggcttttcg gtgctgcgcg atcccgtcgc cgcgcagtcg ccggagtcgg tcggcacgtg gcgctggggc ggcgcgtacg ggcatgcgtg gttcgtcgat cgcgcggccg gactcacggt cgtcgcgctg acgaacacgc tgtacgaggg cgcgcacggc cgattcgtca ccgacgtgcg cgatgcggtc tacggcgcgg ggagggcggc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat