SSGCID: Target Page

BumuA.00102.a

2-dehydro-3-deoxyphosphooctonate aldolase

CENTER ID:	BumuA.00102.a
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00102.a.A1.GE32152	Full length( BumuA.00102.a )		1	284

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00102.a.A1.PW32070	Full length( BumuA.00102.a )		1	284

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.1340
RefSeq:	YP_001579349.1
UniProt:	A9AGW1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKLCDFEVGL DKPFFLIAGT CVVESEQMTI DTAGRLKEIC AKLNIPFIYK SSYDKANRSS GKSFRGLGMD EGLRILSEVK RQLGLPVLTD VHSIDEIEQV ASVVDVLQTP AFLCRQTDFI HACARSGKPV NIKKGQFLAP HDMKNVIDKA RDAAREAGLS EDRFMACERG VSFGYNNLVS DMRSLAIMRE TNAPVVFDAT HSVQLPGGQG TSSGGQREFV PVLARAAVAT GVAGLFMETH PNPAEAKSDG PNAVPLNRMS ALLETLVTLD QAVKRGPFLE NDFN

NT Sequence

ATGAAGCTGT GCGATTTCGA GGTCGGTCTC GACAAGCCTT TCTTCCTGAT CGCGGGTACC TGCGTCGTCG AATCGGAGCA AATGACGATC GACACCGCCG GCCGCCTGAA GGAAATCTGC GCGAAGCTGA ACATTCCGTT CATCTACAAG TCGTCCTATG ACAAGGCGAA CCGCAGTTCC GGCAAGTCGT TTCGCGGCCT CGGAATGGAC GAGGGGCTGC GGATCCTGTC CGAGGTGAAG CGCCAGCTCG GCCTGCCGGT GCTGACCGAC GTGCATTCGA TCGACGAGAT CGAGCAGGTC GCGTCGGTCG TCGACGTGCT GCAGACGCCG GCGTTCCTGT GCCGCCAGAC GGACTTCATC CACGCGTGCG CGCGCTCGGG CAAGCCGGTC AACATCAAGA AAGGCCAGTT TCTCGCGCCG CACGACATGA AGAACGTGAT CGACAAGGCG CGCGACGCCG CGCGTGAAGC CGGTCTGTCG GAAGACCGCT TCATGGCGTG CGAGCGCGGC GTTTCGTTCG GCTACAACAA CCTCGTGTCC GACATGCGCT CGCTCGCGAT CATGCGCGAG ACGAACGCGC CGGTCGTGTT CGACGCGACG CATTCGGTGC AGTTGCCGGG CGGGCAGGGC ACGAGCTCGG GCGGCCAGCG CGAATTCGTG CCGGTGCTCG CACGTGCTGC GGTCGCGACC GGCGTCGCGG GGCTGTTCAT GGAAACGCAT CCGAATCCGG CCGAAGCGAA GTCGGACGGT CCGAATGCGG TGCCGCTGAA CCGGATGAGC GCGCTGCTCG AAACGCTCGT CACGCTCGAC CAGGCTGTGA AGCGCGGTCC GTTCCTGGAA AACGATTTCA AT

Details for BumuA.00102.a.A1.GE32152

HARVESTED ON:	4/26/2011
SEQUENCED ON:	5/2/2011
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMKLCDFE VGLDKPFFLI AGTCVVESEQ MTIDTAGRLK EICAKLNIPF IYKSSYDKAN RSSGKSFRGL GMDEGLRILS EVKRQLGLPV LTDVHSIDEI EQVASVVDVL QTPAFLCRQT DFIHACARSG KPVNIKKGQF LAPHDMKNVI DKARDAAREA GLSEDRFMAC ERGVSFGYNN LVSDMRSLAI MRETNAPVVF DATHSVQLPG GQGTSSGGQR EFVPVLARAA VATGVAGLFM ETHPNPAEAK SDGPNAVPLN RMSALLETLV TLDQAVKRGP FLENDFN

Validated NT Sequence

ttattaattg aaatcgtttt ccaggaacgg accgcgcttc acagcctggt cgagcgtgac gagcgtttcg agcagcgcgc tcatccggtt cagcggcacc gcattcggac cgtccgactt cgcttcggcc ggattcggat gcgtttccat gaacagcccc gcgacgccgg tcgcgaccgc agcacgtgcg agcaccggca cgaattcgcg ctggccgccc gagctcgtgc cctgcccgcc cggcaactgc accgaatgcg tcgcgtcgaa cacgaccggc gcgttcgtct cgcgcatgat cgcgagcgag cgcatgtcgg acacgaggtt gttgtagccg aacgaaacgc cgcgctcgca cgccatgaag cggtcttccg acagaccggc ttcacgcgcg gcgtcgcgcg ccttgtcgat cacgttcttc atgtcgtgcg gcgcgagaaa ctggcctttc ttgatgttga ccggcttgcc cgagcgcgcg cacgcgtgga tgaagtccgt ctggcggcac aggaacgccg gcgtctgcag cacgtcgacg accgacgcga cctgctcgat ctcgtcgatc gaatgcacgt cggtcagcac cggcaggccg agctggcgct tcacctcgga caggatccgc agcccctcgt ccattccgag gccgcgaaac gacttgccgg aactgcggtt cgccttgtca taggacgact tgtagatgaa cggaatgttc agcttcgcgc agatttcctt caggcggccg gcggtgtcga tcgtcatttg ctccgattcg acgacgcagg tacccgcgat caggaagaaa ggcttgtcga gaccgacctc gaaatcgcac agcttcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKLCDFEVG LDKPFFLIAG TCVVESEQMT IDTAGRLKEI CAKLNIPFIY KSSYDKANRS SGKSFRGLGM DEGLRILSEV KRQLGLPVLT DVHSIDEIEQ VASVVDVLQT PAFLCRQTDF IHACARSGKP VNIKKGQFLA PHDMKNVIDK ARDAAREAGL SEDRFMACER GVSFGYNNLV SDMRSLAIMR ETNAPVVFDA THSVQLPGGQ GTSSGGQREF VPVLARAAVA TGVAGLFMET HPNPAEAKSD GPNAVPLNRM SALLETLVTL DQAVKRGPFL ENDFN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagctgtgc gatttcgagg tcggtctcga caagcctttc ttcctgatcg cgggtacctg cgtcgtcgaa tcggagcaaa tgacgatcga caccgccggc cgcctgaagg aaatctgcgc gaagctgaac attccgttca tctacaagtc gtcctatgac aaggcgaacc gcagttccgg caagtcgttt cgcggcctcg gaatggacga ggggctgcgg atcctgtccg aggtgaagcg ccagctcggc ctgccggtgc tgaccgacgt gcattcgatc gacgagatcg agcaggtcgc gtcggtcgtc gacgtgctgc agacgccggc gttcctgtgc cgccagacgg acttcatcca cgcgtgcgcg cgctcgggca agccggtcaa catcaagaaa ggccagtttc tcgcgccgca cgacatgaag aacgtgatcg acaaggcgcg cgacgccgcg cgtgaagccg gtctgtcgga agaccgcttc atggcgtgcg agcgcggcgt ttcgttcggc tacaacaacc tcgtgtccga catgcgctcg ctcgcgatca tgcgcgagac gaacgcgccg gtcgtgttcg acgcgacgca ttcggtgcag ttgccgggcg ggcagggcac gagctcgggc ggccagcgcg aattcgtgcc ggtgctcgca cgtgctgcgg tcgcgaccgg cgtcgcgggg ctgttcatgg aaacgcatcc gaatccggcc gaagcgaagt cggacggtcc gaatgcggtg ccgctgaacc ggatgagcgc gctgctcgaa acgctcgtca cgctcgacca ggctgtgaag cgcggtccgt tcctggaaaa cgatttcaat taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for BumuA.00102.a.A1.PW32070

PURIFICATION DATe:	6/17/2011
CONCENTRATION:	21.07mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	23
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAGCTGTGC GATTTCGAGG TCGGTCTCGA CAAGCCTTTC TTCCTGATCG CGGGTACCTG CGTCGTCGAA TCGGAGCAAA TGACGATCGA CACCGCCGGC CGCCTGAAGG AAATCTGCGC GAAGCTGAAC ATTCCGTTCA TCTACAAGTC GTCCTATGAC AAGGCGAACC GCAGTTCCGG CAAGTCGTTT CGCGGCCTCG GAATGGACGA GGGGCTGCGG ATCCTGTCCG AGGTGAAGCG CCAGCTCGGC CTGCCGGTGC TGACCGACGT GCATTCGATC GACGAGATCG AGCAGGTCGC GTCGGTCGTC GACGTGCTGC AGACGCCGGC GTTCCTGTGC CGCCAGACGG ACTTCATCCA CGCGTGCGCG CGCTCGGGCA AGCCGGTCAA CATCAAGAAA GGCCAGTTTC TCGCGCCGCA CGACATGAAG AACGTGATCG ACAAGGCGCG CGACGCCGCG CGTGAAGCCG GTCTGTCGGA AGACCGCTTC ATGGCGTGCG AGCGCGGCGT TTCGTTCGGC TACAACAACC TCGTGTCCGA CATGCGCTCG CTCGCGATCA TGCGCGAGAC GAACGCGCCG GTCGTGTTCG ACGCGACGCA TTCGGTGCAG TTGCCGGGCG GGCAGGGCAC GAGCTCGGGC GGCCAGCGCG AATTCGTGCC GGTGCTCGCA CGTGCTGCGG TCGCGACCGG CGTCGCGGGG CTGTTCATGG AAACGCATCC GAATCCGGCC GAAGCGAAGT CGGACGGTCC GAATGCGGTG CCGCTGAACC GGATGAGCGC GCTGCTCGAA ACGCTCGTCA CGCTCGACCA GGCTGTGAAG CGCGGTCCGT TCCTGGAAAA CGATTTCAAT TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA