SSGCID: Target Page

BumuA.00096.b

8-amino-7-oxononanoate synthase

CENTER ID:	BumuA.00096.b
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00096.b.B1.GE39680	Full length( BumuA.00096.b )		1	450

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00096.b.B1.PS37882	Full length( BumuA.00096.b )		1	450

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.2830
RefSeq:	YP_001580782.1
UniProt:	A0A0H3KCH3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKTKTPGLP GLTSALKNKL IQQALERKLR EAGRDQPTAS PVAGRKTASS VPEAYTRFDQ YPGYRQLRIM NDGAARLGVR SPFFKLHEGT AGAQTEIAGN TYINYASYNY LDMSGDPVVS QAAKDAIDRY GTSVSASRLV SGERPIHREL ERTLADLYGV DDAITFVSGH AANVTTIGYL FGPRDLVIHD ELIHNSVLQG IQLSGAKRFS FAHNDADALE RLLTEQRHQF ERVLIVLEGI YSMDGDYPDL PRFVDIKRRH RAFLMVDEAH SMGVMGKTGR GIREHFGLAG DAVDIWMGTL SKSLAGCGGY IAGETALVEH LKFLAPGFLY SVGMPPPVAA ASLAALRRML ELPERVATLQ ARGQYFLAQA KAAGIDTGSS AGIAVIPAIL GSSLRATKWS EALFQQGINV QPILYPAVPE QSARLRFFIS CAHSETEIDK VIACMRRLKD

NT Sequence

ATGAGCAAGA CCAAAACCCC CGGCCTGCCG GGCCTGACCT CGGCCCTGAA GAACAAGCTC ATCCAGCAGG CACTCGAGCG CAAGCTGCGC GAAGCCGGGC GTGACCAGCC CACGGCTTCG CCCGTCGCCG GCCGAAAAAC GGCCTCGTCG GTGCCCGAGG CGTACACGCG CTTCGACCAG TATCCGGGCT ACCGCCAGCT GCGCATCATG AACGACGGCG CCGCCCGGCT CGGCGTGCGC AGCCCGTTCT TTAAGCTGCA CGAAGGCACC GCCGGCGCGC AGACCGAAAT CGCAGGCAAT ACGTATATCA ATTACGCGAG CTACAACTAC CTCGACATGT CGGGCGACCC GGTTGTCTCG CAGGCGGCCA AGGACGCGAT CGACCGATAC GGCACATCGG TATCTGCCAG CCGCCTGGTC TCGGGCGAAC GCCCGATTCA CCGCGAACTA GAGCGCACCC TCGCCGATCT GTACGGCGTG GACGACGCGA TCACATTCGT GAGCGGCCAT GCGGCCAACG TGACCACGAT CGGTTATCTG TTCGGGCCGC GCGATCTGGT GATCCACGAC GAGCTAATCC ACAACAGCGT GCTCCAGGGG ATCCAGCTTT CCGGCGCGAA GCGCTTTTCG TTCGCGCACA ACGACGCTGA TGCGCTTGAA CGGTTACTGA CCGAGCAGCG GCACCAGTTC GAACGCGTGC TGATCGTGTT GGAAGGCATC TACAGCATGG ACGGCGACTA CCCCGACCTG CCGCGTTTCG TCGACATTAA GCGCCGCCAT CGGGCATTTC TGATGGTGGA CGAAGCGCAT TCGATGGGCG TGATGGGCAA GACGGGGCGC GGGATTCGCG AGCACTTTGG CCTCGCCGGC GATGCCGTCG ATATCTGGAT GGGTACGCTG AGCAAATCGC TGGCCGGCTG CGGCGGTTAT ATCGCCGGCG AGACGGCGCT CGTCGAGCAT CTGAAGTTTC TCGCGCCGGG GTTCCTGTAC AGCGTGGGCA TGCCGCCGCC GGTGGCCGCG GCGTCCCTCG CGGCGCTGCG GCGCATGCTG GAGTTGCCAG AGCGCGTGGC GACGCTGCAG GCGCGCGGAC AGTATTTTCT TGCCCAGGCC AAGGCCGCGG GGATCGATAC GGGCAGCAGT GCGGGGATAG CGGTCATTCC GGCGATTCTG GGCAGTTCGC TGCGGGCCAC CAAGTGGTCG GAGGCGCTGT TCCAGCAGGG TATCAATGTG CAGCCGATTC TGTATCCGGC CGTGCCGGAA CAGTCGGCGC GGTTGCGGTT CTTCATCTCC TGCGCCCACA GTGAAACGGA GATTGATAAA GTCATTGCTT GCATGCGACG ATTGAAGGAT

Details for BumuA.00096.b.B1.GE39680

HARVESTED ON:	12/3/2015
SEQUENCED ON:	12/7/2015
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	43

Validated AA Sequence

MAHHHHHHMX XTKTPGLPGL TSALKNKLIQ QALERKLREA GRDQPTASPV AGRKTASSVP EAYTRFDQYP GYRQLRIMND GAARLGVRSP FFKLHEGTAG AQTEIAGNTY INYASYNYLD MSGDPVVSQA AKDAIDRYGT SVSASRLVSG ERPIHRELXR TLADLYGVDD AITFVSGHAA NVTTIGYLFG PRDLVXPRRA

Validated NT Sequence

ctnnnnnnnt tatcaatctc cgtttcactg tgggcgcagg agatgaagaa ccgcaaccgc gccgactgtt ccggcacggc cggatacaga atcggctgca nnnnnatacc ctgctggaac agcgcctccg accacttggt ggcccgcagc gaactgccca gaatcgccgg aatgaccgct atccccgcac tgctgcccgt atcgatcccc gcggccttgg cctgggcaag aaaatactgt ccgcgcgcct gcagcgtcgc cacgcgctct ggcaactcca gcatgcgccg cagcgccgcg agggacgccg cggccaccgg cggcggcatg cccacgctgt acaggaaccc cggcgcgaga aacttcagat gctcgacgag cgccgtctcg ccggcgatat aaccgccgca gccggccagc gatttgctca gcgtacccat ccagatatcg acggcatcgc cggcgaggcc aaagtgctcg cgaatcccgc gccccgtctt gcccatcacg cccatcgaat gcgcttcgtc caccatcaga aatgcccgat ggcggcgctt aatgtcgacg aaacgcggca ggtcggggta gtcgccgtcc atgctgtaga tgccttccaa cacgatcagc acgcgttcga actggtgccg ctgctcggtc agtaaccgtt caagcgcatc agcgtcgttg tgcgcgaacg aaaagcgctt cgcgccggga aagctggatc cccctggagc ncgctgttgt ggattaggct cgtcgtgggn tnaccagatc gcgcggcccg aacagataac cgatcgtggt cacgttggcc gcatggccgc tcacgaatgt gatcgcgtcg tccacgccgt acagatcggc gagggtgcgc nnnagttcgc ggtgaatcgg gcgttcgccc gagaccaggc ggctggcaga taccgatgtg ccgtatcggt cgatcgcgtc cttggccgcc tgcgagacaa ccgggtcgcc cgacatgtcg aggtagttgt agctcgcgta attgatatac gtattgcctg cgatttcggt ctgcgcgccg gcggtgcctt cgtgcagctt aaagaacggg ctgcgcacgc cgagccgggc ggcgccgtcg ttcatgatgc gcagctggcg gtagcccgga tactggtcga agcgcgtgta cgcctcgggc accgacgagg ccgtttttcg gccggcgacg ggcgaagccg tgggctggtc acgcccggct tcgcgcagct tgcgctcgag tgcctgctgg atgagcttgt tcttcagggc cgaggtcagg cccggcaggc cgggggtttt ggtcnnnctc atatggtggt ggtggtggtg agccatg

Expected Protein Sequence

MAHHHHHHMS KTKTPGLPGL TSALKNKLIQ QALERKLREA GRDQPTASPV AGRKTASSVP EAYTRFDQYP GYRQLRIMND GAARLGVRSP FFKLHEGTAG AQTEIAGNTY INYASYNYLD MSGDPVVSQA AKDAIDRYGT SVSASRLVSG ERPIHRELER TLADLYGVDD AITFVSGHAA NVTTIGYLFG PRDLVIHDEL IHNSVLQGIQ LSGAKRFSFA HNDADALERL LTEQRHQFER VLIVLEGIYS MDGDYPDLPR FVDIKRRHRA FLMVDEAHSM GVMGKTGRGI REHFGLAGDA VDIWMGTLSK SLAGCGGYIA GETALVEHLK FLAPGFLYSV GMPPPVAAAS LAALRRMLEL PERVATLQAR GQYFLAQAKA AGIDTGSSAG IAVIPAILGS SLRATKWSEA LFQQGINVQP ILYPAVPEQS ARLRFFISCA HSETEIDKVI ACMRRLKD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca agaccaaaac ccccggcctg ccgggcctga cctcggccct gaagaacaag ctcatccagc aggcactcga gcgcaagctg cgcgaagccg ggcgtgacca gcccacggct tcgcccgtcg ccggccgaaa aacggcctcg tcggtgcccg aggcgtacac gcgcttcgac cagtatccgg gctaccgcca gctgcgcatc atgaacgacg gcgccgcccg gctcggcgtg cgcagcccgt tctttaagct gcacgaaggc accgccggcg cgcagaccga aatcgcaggc aatacgtata tcaattacgc gagctacaac tacctcgaca tgtcgggcga cccggttgtc tcgcaggcgg ccaaggacgc gatcgaccga tacggcacat cggtatctgc cagccgcctg gtctcgggcg aacgcccgat tcaccgcgaa ctagagcgca ccctcgccga tctgtacggc gtggacgacg cgatcacatt cgtgagcggc catgcggcca acgtgaccac gatcggttat ctgttcgggc cgcgcgatct ggtgatccac gacgagctaa tccacaacag cgtgctccag gggatccagc tttccggcgc gaagcgcttt tcgttcgcgc acaacgacgc tgatgcgctt gaacggttac tgaccgagca gcggcaccag ttcgaacgcg tgctgatcgt gttggaaggc atctacagca tggacggcga ctaccccgac ctgccgcgtt tcgtcgacat taagcgccgc catcgggcat ttctgatggt ggacgaagcg cattcgatgg gcgtgatggg caagacgggg cgcgggattc gcgagcactt tggcctcgcc ggcgatgccg tcgatatctg gatgggtacg ctgagcaaat cgctggccgg ctgcggcggt tatatcgccg gcgagacggc gctcgtcgag catctgaagt ttctcgcgcc ggggttcctg tacagcgtgg gcatgccgcc gccggtggcc gcggcgtccc tcgcggcgct gcggcgcatg ctggagttgc cagagcgcgt ggcgacgctg caggcgcgcg gacagtattt tcttgcccag gccaaggccg cggggatcga tacgggcagc agtgcgggga tagcggtcat tccggcgatt ctgggcagtt cgctgcgggc caccaagtgg tcggaggcgc tgttccagca gggtatcaat gtgcagccga ttctgtatcc ggccgtgccg gaacagtcgg cgcggttgcg gttcttcatc tcctgcgccc acagtgaaac ggagattgat aaagtcattg cttgcatgcg acgattgaag gattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for BumuA.00096.b.B1.PS37882

PURIFICATION DATe:	3/15/2016
CONCENTRATION:	57mg/ml
OBSERVED MW:	48kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	43

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA AGACCAAAAC CCCCGGCCTG CCGGGCCTGA CCTCGGCCCT GAAGAACAAG CTCATCCAGC AGGCACTCGA GCGCAAGCTG CGCGAAGCCG GGCGTGACCA GCCCACGGCT TCGCCCGTCG CCGGCCGAAA AACGGCCTCG TCGGTGCCCG AGGCGTACAC GCGCTTCGAC CAGTATCCGG GCTACCGCCA GCTGCGCATC ATGAACGACG GCGCCGCCCG GCTCGGCGTG CGCAGCCCGT TCTTTAAGCT GCACGAAGGC ACCGCCGGCG CGCAGACCGA AATCGCAGGC AATACGTATA TCAATTACGC GAGCTACAAC TACCTCGACA TGTCGGGCGA CCCGGTTGTC TCGCAGGCGG CCAAGGACGC GATCGACCGA TACGGCACAT CGGTATCTGC CAGCCGCCTG GTCTCGGGCG AACGCCCGAT TCACCGCGAA CTAGAGCGCA CCCTCGCCGA TCTGTACGGC GTGGACGACG CGATCACATT CGTGAGCGGC CATGCGGCCA ACGTGACCAC GATCGGTTAT CTGTTCGGGC CGCGCGATCT GGTGATCCAC GACGAGCTAA TCCACAACAG CGTGCTCCAG GGGATCCAGC TTTCCGGCGC GAAGCGCTTT TCGTTCGCGC ACAACGACGC TGATGCGCTT GAACGGTTAC TGACCGAGCA GCGGCACCAG TTCGAACGCG TGCTGATCGT GTTGGAAGGC ATCTACAGCA TGGACGGCGA CTACCCCGAC CTGCCGCGTT TCGTCGACAT TAAGCGCCGC CATCGGGCAT TTCTGATGGT GGACGAAGCG CATTCGATGG GCGTGATGGG CAAGACGGGG CGCGGGATTC GCGAGCACTT TGGCCTCGCC GGCGATGCCG TCGATATCTG GATGGGTACG CTGAGCAAAT CGCTGGCCGG CTGCGGCGGT TATATCGCCG GCGAGACGGC GCTCGTCGAG CATCTGAAGT TTCTCGCGCC GGGGTTCCTG TACAGCGTGG GCATGCCGCC GCCGGTGGCC GCGGCGTCCC TCGCGGCGCT GCGGCGCATG CTGGAGTTGC CAGAGCGCGT GGCGACGCTG CAGGCGCGCG GACAGTATTT TCTTGCCCAG GCCAAGGCCG CGGGGATCGA TACGGGCAGC AGTGCGGGGA TAGCGGTCAT TCCGGCGATT CTGGGCAGTT CGCTGCGGGC CACCAAGTGG TCGGAGGCGC TGTTCCAGCA GGGTATCAAT GTGCAGCCGA TTCTGTATCC GGCCGTGCCG GAACAGTCGG CGCGGTTGCG GTTCTTCATC TCCTGCGCCC ACAGTGAAAC GGAGATTGAT AAAGTCATTG CTTGCATGCG ACGATTGAAG GATTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT