BumuA.00096.a
8-amino-7-oxononanoate synthase
| CENTER ID: | BumuA.00096.a |
| ORGANISM: | Burkholderia multivorans ATCC 17616 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00096.a.B1.GE39679 | Full length( BumuA.00096.a ) | 1 | 394 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00096.a.B1.PW37876 | Full length( BumuA.00096.a ) | 1 | 394 |
Structures
5VNX
DEPOSITED: 5/1/2017
DETERMINATION: XRay
CLONE: BumuA.00096.a.B1.GE39679
PROTEIN: BumuA.00096.a.B1.PW37876
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|395019.8.peg.505 |
| RefSeq: | YP_001578565.1 |
| UniProt: | A9AE46 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLLDTLQRG LADLDAQGLR RVRRTADSAC DAHMTVNGRE IVGFASNDYL GLAAHPKLVA AFAEGAQRYG SGSGGSHLLG GHSRAHAKLE DELAGFAGGF SDAPRALYFS TGYMANLAAV TALAGKDATI FSDALNHASL IDGTRLSRAT VQVYPHADTA TLGALLEACT SQTKLIVTDT VFSMDGDIAP LAELLALAER HGAWLVVDDA HGFGVLGPQG RGALAAAALR SPHLVYVGTL GKAAGVAGAF VVAHETVIEW LIQRARSYIF TTAAPPAVAH AVSASLKVIA GDEGDARRAH LAALIERTRA LLRRTRWQPV DSHTAVQPLV IGSNEATLAA MRALDAHGLW VPAIRPPTVP AGTSRLRISL SAAHSFDDLA RLETALLRAS EEAA
NT Sequence
ATGAACCTGC TCGATACGCT GCAGCGCGGC CTCGCCGATC TCGACGCGCA AGGGCTGCGC CGTGTGCGCC GCACGGCCGA CAGCGCCTGC GACGCGCACA TGACCGTGAA CGGCCGCGAG ATCGTCGGGT TCGCGAGCAA CGACTACCTC GGCCTCGCCG CGCATCCGAA ACTTGTCGCC GCGTTCGCCG AAGGCGCGCA GCGCTACGGC TCCGGCAGCG GCGGCTCGCA TCTGCTCGGC GGCCACTCGC GTGCGCACGC GAAGCTCGAG GACGAACTCG CGGGCTTCGC GGGCGGCTTC TCCGATGCGC CGCGCGCGCT GTACTTCAGC ACCGGCTACA TGGCGAACCT CGCGGCGGTC ACCGCGCTCG CCGGCAAGGA CGCGACGATC TTCTCCGACG CGCTGAACCA CGCGTCGCTG ATCGACGGCA CGCGGCTGTC GCGCGCCACC GTGCAGGTCT ATCCGCATGC CGATACGGCC ACGCTCGGCG CGTTGCTCGA GGCATGCACG TCGCAGACGA AGCTGATCGT CACCGATACG GTGTTCAGCA TGGACGGCGA CATCGCGCCG CTCGCCGAAC TCCTCGCGCT CGCGGAACGC CACGGCGCAT GGCTCGTCGT CGACGACGCG CACGGCTTCG GCGTGCTCGG CCCGCAAGGC CGCGGCGCGC TCGCGGCGGC CGCGCTGCGC TCGCCGCATC TCGTCTACGT CGGCACGCTC GGCAAGGCGG CCGGCGTCGC GGGCGCATTC GTCGTCGCGC ACGAGACGGT GATCGAGTGG CTGATCCAGC GCGCGCGCAG CTACATCTTC ACGACGGCCG CACCGCCGGC CGTCGCGCAT GCGGTGTCGG CGAGCCTGAA GGTGATCGCC GGCGACGAAG GCGATGCGCG GCGCGCGCAC CTCGCCGCGC TGATCGAACG CACGCGCGCG CTGCTGCGCC GCACGCGCTG GCAGCCGGTC GACTCGCACA CGGCCGTGCA GCCGCTCGTG ATCGGCAGCA ACGAGGCGAC GCTCGCCGCG ATGCGCGCGC TCGACGCGCA CGGCCTGTGG GTGCCCGCGA TCCGGCCGCC GACGGTGCCG GCCGGCACCT CGCGGCTACG CATCTCGCTG TCGGCCGCGC ATTCGTTCGA CGATCTCGCG CGGCTCGAAA CCGCGCTGCT GCGCGCAAGC GAGGAGGCCG CA
Details for BumuA.00096.a.B1.GE39679
| HARVESTED ON: | 12/3/2015 |
| SEQUENCED ON: | 12/7/2015 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 61 |
| PERCENT COVERAGE: | 65 |
Validated AA Sequence
MAHHHHHHMN LLDTLQRGLA DLDAQGLRRV RRTAXXACDA HMTVNGREIV GFASNDYLGL AAHPKLVAAF AEGAQRYGSG SGGSHLLGGH SRAHAKLEXX LXGFAGGFSD APRALYFXXG YMANLAAVTA LAXXDAXXFS DALNHXXXIX XHAAVAXXRA GLSACRYGXX RRVARGMHVA XEXXXXRYGV QHGRRHRAAR XXXRAXXTPR RXGSSXXTRX XXACSARKAA ARSRRPRXAR RXXXTSARSA RRPASRAHSS SRTRR
Validated NT Sequence
gtttcgagcc gcgcgagatc gtcgaacgaa tgcgcggccg acagcgagat gcgtagccgc gaggtgccgg ccggcaccgt cggcggccgg atcgcgggca cccacaggcc gtgcgcgtcg agcgcgcgca tcgcggcgag cgtcgcctcg ttgctgccga tcacgagcgg ctgcacggcc gtgtgcgagt cgaccggctg ccagcgcgtg cggcgcagca gcgcgcgcgt gcgttcgatc agcgcggcga ggtgcgcgcg ccgcgcatcg ccttcgtcgc cggcgatcac cttcaggctc gccgacaccg catgcgcgac ggccggcggt gcggccgtcg tgaagatgta gctgcnnncg cgctggatca gccactcgat caccgtctcg tgcgcgacga cgaatgcgcc cgcgacgccg gccgccttgc cgagcgtgcc gacgtnnncn nnntgcggcg agcgcngcgn ggccgccgcg agcgcgccgc ggccttgcgg gccgagcacg cnnnnnnnnt gcgcgtcnnn nncgacgagc catngcgccg tggcgttcnn nnngcgcgag nnnntnggcg agcggcgcga tgtcgccgtc catgctgaac accgtatcgg tnnnnntcan cttcnnntgc gacgtgcatg cctcgagcaa cgcgccgann ntggccgtat cggcatgcgg atagacctgc acggnnnngc gcgacagccg cgtgcnnnnn gatcannnnn ncgtggttca gcgcgtcgga gaagnnnnnn gcgtcctnnn cggcgagcgc ggtgaccgcc gcgaggttcg ccatgtagcc gnnnnngaag tacagcgcgc gcggcgcatc ggagaagccg cccgcgaagc ccnnnagtnn ntcctcgagc ttcgcgtgcg cacgcgagtg gccgccgagc agatgcgagc cgccgctgcc ggagccgtag cgctgcgcgc cttcggcgaa cgcggcgaca agtttcggat gcgcggcgag gccgaggtag tcgttgctcg cgaacccgac gatctcgcgg ccgttcacgg tcatgtgcgc gtcgcaggcg cnntcggccg tgcggcgcac acggcgcagc ccttgcgcgt cgagatcggc gaggccgcgc tgcagcgtat cgagcaggtt catatggtgg tggtggtggt gagccatggt
Expected Protein Sequence
MAHHHHHHMN LLDTLQRGLA DLDAQGLRRV RRTADSACDA HMTVNGREIV GFASNDYLGL AAHPKLVAAF AEGAQRYGSG SGGSHLLGGH SRAHAKLEDE LAGFAGGFSD APRALYFSTG YMANLAAVTA LAGKDATIFS DALNHASLID GTRLSRATVQ VYPHADTATL GALLEACTSQ TKLIVTDTVF SMDGDIAPLA ELLALAERHG AWLVVDDAHG FGVLGPQGRG ALAAAALRSP HLVYVGTLGK AAGVAGAFVV AHETVIEWLI QRARSYIFTT AAPPAVAHAV SASLKVIAGD EGDARRAHLA ALIERTRALL RRTRWQPVDS HTAVQPLVIG SNEATLAAMR ALDAHGLWVP AIRPPTVPAG TSRLRISLSA AHSFDDLARL ETALLRASEE AA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc tgctcgatac gctgcagcgc ggcctcgccg atctcgacgc gcaagggctg cgccgtgtgc gccgcacggc cgacagcgcc tgcgacgcgc acatgaccgt gaacggccgc gagatcgtcg ggttcgcgag caacgactac ctcggcctcg ccgcgcatcc gaaacttgtc gccgcgttcg ccgaaggcgc gcagcgctac ggctccggca gcggcggctc gcatctgctc ggcggccact cgcgtgcgca cgcgaagctc gaggacgaac tcgcgggctt cgcgggcggc ttctccgatg cgccgcgcgc gctgtacttc agcaccggct acatggcgaa cctcgcggcg gtcaccgcgc tcgccggcaa ggacgcgacg atcttctccg acgcgctgaa ccacgcgtcg ctgatcgacg gcacgcggct gtcgcgcgcc accgtgcagg tctatccgca tgccgatacg gccacgctcg gcgcgttgct cgaggcatgc acgtcgcaga cgaagctgat cgtcaccgat acggtgttca gcatggacgg cgacatcgcg ccgctcgccg aactcctcgc gctcgcggaa cgccacggcg catggctcgt cgtcgacgac gcgcacggct tcggcgtgct cggcccgcaa ggccgcggcg cgctcgcggc ggccgcgctg cgctcgccgc atctcgtcta cgtcggcacg ctcggcaagg cggccggcgt cgcgggcgca ttcgtcgtcg cgcacgagac ggtgatcgag tggctgatcc agcgcgcgcg cagctacatc ttcacgacgg ccgcaccgcc ggccgtcgcg catgcggtgt cggcgagcct gaaggtgatc gccggcgacg aaggcgatgc gcggcgcgcg cacctcgccg cgctgatcga acgcacgcgc gcgctgctgc gccgcacgcg ctggcagccg gtcgactcgc acacggccgt gcagccgctc gtgatcggca gcaacgaggc gacgctcgcc gcgatgcgcg cgctcgacgc gcacggcctg tgggtgcccg cgatccggcc gccgacggtg ccggccggca cctcgcggct acgcatctcg ctgtcggccg cgcattcgtt cgacgatctc gcgcggctcg aaaccgcgct gctgcgcgca agcgaggagg ccgcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BumuA.00096.a.B1.PW37876
| PURIFICATION DATe: | 3/22/2016 |
| CONCENTRATION: | 33mg/ml |
| OBSERVED MW: | 41kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 61 |
| PERCENT COVERAGE: | 65 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN LLDTLQRGLA DLDAQGLRRV RRTAXXACDA HMTVNGREIV GFASNDYLGL AAHPKLVAAF AEGAQRYGSG SGGSHLLGGH SRAHAKLEXX LXGFAGGFSD APRALYFXXG YMANLAAVTA LAXXDAXXFS DALNHXXXIX XHAAVAXXRA GLSACRYGXX RRVARGMHVA XEXXXXRYGV QHGRRHRAAR XXXRAXXTPR RXGSSXXTRX XXACSARKAA ARSRRPRXAR RXXXTSARSA RRPASRAHSS SRTRR
Validated NT Sequence
gtttcgagcc gcgcgagatc gtcgaacgaa tgcgcggccg acagcgagat gcgtagccgc gaggtgccgg ccggcaccgt cggcggccgg atcgcgggca cccacaggcc gtgcgcgtcg agcgcgcgca tcgcggcgag cgtcgcctcg ttgctgccga tcacgagcgg ctgcacggcc gtgtgcgagt cgaccggctg ccagcgcgtg cggcgcagca gcgcgcgcgt gcgttcgatc agcgcggcga ggtgcgcgcg ccgcgcatcg ccttcgtcgc cggcgatcac cttcaggctc gccgacaccg catgcgcgac ggccggcggt gcggccgtcg tgaagatgta gctgcnnncg cgctggatca gccactcgat caccgtctcg tgcgcgacga cgaatgcgcc cgcgacgccg gccgccttgc cgagcgtgcc gacgtnnncn nnntgcggcg agcgcngcgn ggccgccgcg agcgcgccgc ggccttgcgg gccgagcacg cnnnnnnnnt gcgcgtcnnn nncgacgagc catngcgccg tggcgttcnn nnngcgcgag nnnntnggcg agcggcgcga tgtcgccgtc catgctgaac accgtatcgg tnnnnntcan cttcnnntgc gacgtgcatg cctcgagcaa cgcgccgann ntggccgtat cggcatgcgg atagacctgc acggnnnngc gcgacagccg cgtgcnnnnn gatcannnnn ncgtggttca gcgcgtcgga gaagnnnnnn gcgtcctnnn cggcgagcgc ggtgaccgcc gcgaggttcg ccatgtagcc gnnnnngaag tacagcgcgc gcggcgcatc ggagaagccg cccgcgaagc ccnnnagtnn ntcctcgagc ttcgcgtgcg cacgcgagtg gccgccgagc agatgcgagc cgccgctgcc ggagccgtag cgctgcgcgc cttcggcgaa cgcggcgaca agtttcggat gcgcggcgag gccgaggtag tcgttgctcg cgaacccgac gatctcgcgg ccgttcacgg tcatgtgcgc gtcgcaggcg cnntcggccg tgcggcgcac acggcgcagc ccttgcgcgt cgagatcggc gaggccgcgc tgcagcgtat cgagcaggtt catatggtgg tggtggtggt gagccatggt
Expressed Protein Sequence
MAHHHHHHMN LLDTLQRGLA DLDAQGLRRV RRTADSACDA HMTVNGREIV GFASNDYLGL AAHPKLVAAF AEGAQRYGSG SGGSHLLGGH SRAHAKLEDE LAGFAGGFSD APRALYFSTG YMANLAAVTA LAGKDATIFS DALNHASLID GTRLSRATVQ VYPHADTATL GALLEACTSQ TKLIVTDTVF SMDGDIAPLA ELLALAERHG AWLVVDDAHG FGVLGPQGRG ALAAAALRSP HLVYVGTLGK AAGVAGAFVV AHETVIEWLI QRARSYIFTT AAPPAVAHAV SASLKVIAGD EGDARRAHLA ALIERTRALL RRTRWQPVDS HTAVQPLVIG SNEATLAAMR ALDAHGLWVP AIRPPTVPAG TSRLRISLSA AHSFDDLARL ETALLRASEE AA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACC TGCTCGATAC GCTGCAGCGC GGCCTCGCCG ATCTCGACGC GCAAGGGCTG CGCCGTGTGC GCCGCACGGC CGACAGCGCC TGCGACGCGC ACATGACCGT GAACGGCCGC GAGATCGTCG GGTTCGCGAG CAACGACTAC CTCGGCCTCG CCGCGCATCC GAAACTTGTC GCCGCGTTCG CCGAAGGCGC GCAGCGCTAC GGCTCCGGCA GCGGCGGCTC GCATCTGCTC GGCGGCCACT CGCGTGCGCA CGCGAAGCTC GAGGACGAAC TCGCGGGCTT CGCGGGCGGC TTCTCCGATG CGCCGCGCGC GCTGTACTTC AGCACCGGCT ACATGGCGAA CCTCGCGGCG GTCACCGCGC TCGCCGGCAA GGACGCGACG ATCTTCTCCG ACGCGCTGAA CCACGCGTCG CTGATCGACG GCACGCGGCT GTCGCGCGCC ACCGTGCAGG TCTATCCGCA TGCCGATACG GCCACGCTCG GCGCGTTGCT CGAGGCATGC ACGTCGCAGA CGAAGCTGAT CGTCACCGAT ACGGTGTTCA GCATGGACGG CGACATCGCG CCGCTCGCCG AACTCCTCGC GCTCGCGGAA CGCCACGGCG CATGGCTCGT CGTCGACGAC GCGCACGGCT TCGGCGTGCT CGGCCCGCAA GGCCGCGGCG CGCTCGCGGC GGCCGCGCTG CGCTCGCCGC ATCTCGTCTA CGTCGGCACG CTCGGCAAGG CGGCCGGCGT CGCGGGCGCA TTCGTCGTCG CGCACGAGAC GGTGATCGAG TGGCTGATCC AGCGCGCGCG CAGCTACATC TTCACGACGG CCGCACCGCC GGCCGTCGCG CATGCGGTGT CGGCGAGCCT GAAGGTGATC GCCGGCGACG AAGGCGATGC GCGGCGCGCG CACCTCGCCG CGCTGATCGA ACGCACGCGC GCGCTGCTGC GCCGCACGCG CTGGCAGCCG GTCGACTCGC ACACGGCCGT GCAGCCGCTC GTGATCGGCA GCAACGAGGC GACGCTCGCC GCGATGCGCG CGCTCGACGC GCACGGCCTG TGGGTGCCCG CGATCCGGCC GCCGACGGTG CCGGCCGGCA CCTCGCGGCT ACGCATCTCG CTGTCGGCCG CGCATTCGTT CGACGATCTC GCGCGGCTCG AAACCGCGCT GCTGCGCGCA AGCGAGGAGG CCGCATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT