SSGCID: Target Page

BumuA.00090.b

3-deoxy-7-phosphoheptulonate synthase

CENTER ID:	BumuA.00090.b
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00090.b.B1.GE39677	Full length( BumuA.00090.b )		1	376

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00090.b.B1.PW37874	Full length( BumuA.00090.b )		1	376

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.3485
RefSeq:	YP_001583208.1
UniProt:	A0A0H3KPX9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQKLDNPTHD REVGVADATL DTTRIDDVRI GAVRPLISPA LLLDELPVPA ATQTLVEDTR KAIGDILHGR DDRLLLVVGP CSIHDHDQAL DYARRLKAAA DALKDDLLIT MRVYFEKPRT TVGWKGYIND PRLDGSFRIN EGLRAARQLL LDINALGLPA STEFLDLLSP QYIADLIAWG AIGARTTESQ SHRQLASGLS CPIGFKNGTD GGVQVASDAI VAARASHAFM GMTKMGMAAI FETRGNDDAH VILRGGKNGP NYDAAHVDAS CAVLRAAGLR EQVMVDCSHA NSNKSHERQI EVAQDLARQL SQGEHRIVGV MIESHLEAGR QDLKPGVPLK YGVSITDACL SWAQTEPVLD VLAEAVRHRR VFARNA

NT Sequence

ATGCAGAAGC TCGACAATCC CACGCACGAT CGCGAGGTCG GCGTCGCCGA CGCGACGCTC GATACGACGC GCATCGACGA CGTCCGTATC GGCGCCGTGC GTCCGCTGAT TTCGCCGGCG CTGCTGCTCG ACGAACTGCC GGTGCCGGCG GCCACGCAGA CGCTCGTCGA AGATACGCGC AAGGCGATCG GCGACATCCT GCACGGTCGC GACGACCGGT TGCTGCTCGT CGTCGGCCCA TGCTCGATCC ACGATCACGA CCAGGCGCTC GACTACGCGC GCCGCCTCAA GGCCGCCGCC GATGCGCTGA AGGACGATCT GCTGATCACG ATGCGCGTGT ACTTCGAGAA GCCGCGCACG ACGGTCGGCT GGAAGGGCTA CATCAACGAT CCGCGTCTCG ACGGCAGCTT CCGCATCAAC GAAGGGCTGC GCGCCGCGCG GCAGCTGCTG CTCGACATCA ACGCGCTCGG CCTGCCCGCC TCGACCGAAT TCCTCGATCT GCTGAGCCCG CAGTACATCG CGGACCTGAT CGCGTGGGGT GCGATCGGCG CACGCACGAC CGAGAGCCAG AGCCATCGTC AGCTCGCGTC GGGGCTCAGC TGCCCGATCG GCTTCAAGAA CGGCACCGAC GGCGGCGTGC AGGTCGCGTC GGACGCGATC GTCGCCGCGC GCGCGAGCCA TGCGTTCATG GGGATGACGA AGATGGGCAT GGCCGCGATC TTCGAGACGC GCGGCAACGA CGACGCTCAT GTGATTTTGC GCGGCGGCAA GAACGGCCCG AACTACGACG CGGCGCACGT CGACGCGAGC TGCGCCGTGC TGCGCGCGGC GGGCCTGCGC GAGCAGGTGA TGGTCGACTG CTCGCATGCG AATTCGAACA AGTCGCACGA GCGGCAGATC GAGGTCGCGC AGGATCTCGC GCGCCAGCTG TCGCAGGGCG AGCACCGCAT CGTCGGCGTG ATGATCGAGA GCCATCTCGA AGCCGGCCGT CAGGATCTGA AGCCCGGCGT GCCGTTGAAG TACGGCGTAT CGATCACCGA TGCATGCCTG AGCTGGGCGC AGACCGAACC GGTGCTCGAC GTGCTCGCCG AAGCCGTGCG TCATCGCCGC GTGTTCGCGC GCAACGCG

Details for BumuA.00090.b.B1.GE39677

HARVESTED ON:	12/3/2015
SEQUENCED ON:	12/7/2015
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMQ KLDNPTHDRE VGVADATLDT TRIDDVRIGA VRPLISPALL LDELPVPAAT QTLVEDTRKA IGDILHGRDD RLLLVVGPCS IHDHDQALDY ARRLKAAADA LKDDLLITMR VYFEKPRTTV GWKGYINDPR LDGSFRINEG LRAARQLLLD INALGLPAST EFLDLLSPQY IADLIAWGAI GARTTESQSH RQLASGLSCP IGFKNGTDGG VQVASDAIVA ARASHAFMGM TKMGMAAIFE TRGNDDAHVI LRGGKNGPNY DAAHVDASCA VLRAAGLREQ VMVDCSHANS NKSHERQIEV AQDLARQLSQ GEHRIVGVMI ESHLEAGRQD LKPGVPLKYG VSITDACLSW AQTEPVLDVL AEAVR

Validated NT Sequence

gacgcacggc ttcggcgagc acgtcgagca ccggttcggt ctgcgcccag ctcaggcatg catcggtgat cgatacgccg tacttcaacg gcacgccggg cttcagatcc tgacggccgg cttcgagatg gctctcgatc atcacgccga cgatgcggtg ctcgccctgc gacagctggc gcgcgagatc ctgcgcgacc tcgatctgcc gctcgtgcga cttgttcgaa ttcgcatgcg agcagtcgac catcacctgc tcgcgcaggc ccgccgcgcg cagcacggcg cagctcgcgt cgacgtgcgc cgcgtcgtag ttcgggccgt tcttgccgcc gcgcaaaatc acatgagcgt cgtcgttgcc gcgcgtctcg aagatcgcgg ccatgcccat cttcgtcatc cccatgaacg catggctcgc gcgcgcggcg acgatcgcgt ccgacgcgac ctgcacgccg ccgtcggtgc cgttcttgaa gccgatcggg cagctgagcc ccgacgcgag ctgacgatgg ctctggctct cggtcgtgcg tgcgccgatc gcaccccacg cgatcaggtc cgcgatgtac tgcgggctca gcagatcgag gaattcggtc gaggcgggca ggccgagcgc gttgatgtcg agcagcagct gccgcgcggc gcgcagccct tcgttgatgc ggaagctgcc gtcgagacgc ggatcgttga tgtagccctt ccagccgacc gtcgtgcgcg gcttctcgaa gtacacgcgc atcgtgatca gcagatcgtc cttcagcgca tcggcggcgg ccttgaggcg gcgcgcgtag tcgagcgcct ggtcgtgatc gtggatcgag catgggccga cgacgagcag caaccggtcg tcgcgaccgt gcaggatgtc gccgatcgcc ttgcgcgtat cttcgacgag cgtctgcgtg gccgccggca ccggcagttc gtcgagcagc agcgccggcg aaatcagcgg acgcacggcg ccgatacgga cgtcgtcgat gcgcgtcgta tcgagcgtcg cgtcggcgac gccgacctcg cgatcgtgcg tgggattgtc gagcttctgc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaga agctcgacaa tcccacgcac gatcgcgagg tcggcgtcgc cgacgcgacg ctcgatacga cgcgcatcga cgacgtccgt atcggcgccg tgcgtccgct gatttcgccg gcgctgctgc tcgacgaact gccggtgccg gcggccacgc agacgctcgt cgaagatacg cgcaaggcga tcggcgacat cctgcacggt cgcgacgacc ggttgctgct cgtcgtcggc ccatgctcga tccacgatca cgaccaggcg ctcgactacg cgcgccgcct caaggccgcc gccgatgcgc tgaaggacga tctgctgatc acgatgcgcg tgtacttcga gaagccgcgc acgacggtcg gctggaaggg ctacatcaac gatccgcgtc tcgacggcag cttccgcatc aacgaagggc tgcgcgccgc gcggcagctg ctgctcgaca tcaacgcgct cggcctgccc gcctcgaccg aattcctcga tctgctgagc ccgcagtaca tcgcggacct gatcgcgtgg ggtgcgatcg gcgcacgcac gaccgagagc cagagccatc gtcagctcgc gtcggggctc agctgcccga tcggcttcaa gaacggcacc gacggcggcg tgcaggtcgc gtcggacgcg atcgtcgccg cgcgcgcgag ccatgcgttc atggggatga cgaagatggg catggccgcg atcttcgaga cgcgcggcaa cgacgacgct catgtgattt tgcgcggcgg caagaacggc ccgaactacg acgcggcgca cgtcgacgcg agctgcgccg tgctgcgcgc ggcgggcctg cgcgagcagg tgatggtcga ctgctcgcat gcgaattcga acaagtcgca cgagcggcag atcgaggtcg cgcaggatct cgcgcgccag ctgtcgcagg gcgagcaccg catcgtcggc gtgatgatcg agagccatct cgaagccggc cgtcaggatc tgaagcccgg cgtgccgttg aagtacggcg tatcgatcac cgatgcatgc ctgagctggg cgcagaccga accggtgctc gacgtgctcg ccgaagccgt gcgtcatcgc cgcgtgttcg cgcgcaacgc gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BumuA.00090.b.B1.PW37874

PURIFICATION DATe:	3/22/2016
CONCENTRATION:	32mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	14
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAGA AGCTCGACAA TCCCACGCAC GATCGCGAGG TCGGCGTCGC CGACGCGACG CTCGATACGA CGCGCATCGA CGACGTCCGT ATCGGCGCCG TGCGTCCGCT GATTTCGCCG GCGCTGCTGC TCGACGAACT GCCGGTGCCG GCGGCCACGC AGACGCTCGT CGAAGATACG CGCAAGGCGA TCGGCGACAT CCTGCACGGT CGCGACGACC GGTTGCTGCT CGTCGTCGGC CCATGCTCGA TCCACGATCA CGACCAGGCG CTCGACTACG CGCGCCGCCT CAAGGCCGCC GCCGATGCGC TGAAGGACGA TCTGCTGATC ACGATGCGCG TGTACTTCGA GAAGCCGCGC ACGACGGTCG GCTGGAAGGG CTACATCAAC GATCCGCGTC TCGACGGCAG CTTCCGCATC AACGAAGGGC TGCGCGCCGC GCGGCAGCTG CTGCTCGACA TCAACGCGCT CGGCCTGCCC GCCTCGACCG AATTCCTCGA TCTGCTGAGC CCGCAGTACA TCGCGGACCT GATCGCGTGG GGTGCGATCG GCGCACGCAC GACCGAGAGC CAGAGCCATC GTCAGCTCGC GTCGGGGCTC AGCTGCCCGA TCGGCTTCAA GAACGGCACC GACGGCGGCG TGCAGGTCGC GTCGGACGCG ATCGTCGCCG CGCGCGCGAG CCATGCGTTC ATGGGGATGA CGAAGATGGG CATGGCCGCG ATCTTCGAGA CGCGCGGCAA CGACGACGCT CATGTGATTT TGCGCGGCGG CAAGAACGGC CCGAACTACG ACGCGGCGCA CGTCGACGCG AGCTGCGCCG TGCTGCGCGC GGCGGGCCTG CGCGAGCAGG TGATGGTCGA CTGCTCGCAT GCGAATTCGA ACAAGTCGCA CGAGCGGCAG ATCGAGGTCG CGCAGGATCT CGCGCGCCAG CTGTCGCAGG GCGAGCACCG CATCGTCGGC GTGATGATCG AGAGCCATCT CGAAGCCGGC CGTCAGGATC TGAAGCCCGG CGTGCCGTTG AAGTACGGCG TATCGATCAC CGATGCATGC CTGAGCTGGG CGCAGACCGA ACCGGTGCTC GACGTGCTCG CCGAAGCCGT GCGTCATCGC CGCGTGTTCG CGCGCAACGC GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT