BumuA.00090.a

3-deoxy-7-phosphoheptulonate synthase

CENTER ID: BumuA.00090.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00090.a.B1.GE39676 Full length( BumuA.00090.a ) 1 357
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00090.a.B1.PS37904 Full length( BumuA.00090.a ) 1 357

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.2873
RefSeq: YP_001580827.1
UniProt: A0A0H3KCD7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPPHNTDDVR IRELKELTPP AHLIREFACS EAASELIYNA RQAMHRILHG MDDRLIVVIG PCSIHDTKAA LEYAGRLVKE RERFKGELEI VMRVYFEKPR TTVGWKGLIN DPHLDNSFKI NEGLRTAREL LLQINEMGLP AGTEYLDMIS PQYIADLISW GAIGARTTES QVHRELASGL SCPVGFKNGT DGNVKIAVDA IKAASQPHHF LSVTKGGHSA IVSTAGNEDC HVILRGGKAP NYDADSVNAA CADIGKAGLA ARLMIDASHA NSSKKHENQI PVCADIGRQV AAGDSRIVGV MIESHLVEGR QDLKEGCELT YGQSITDACI NWEDSVKVLE GLAESVKARR VASGSGN
NT Sequence
ATGCCCCCGC ACAATACCGA CGACGTCCGC ATCCGCGAAC TCAAGGAACT GACGCCGCCC GCTCACCTGA TCCGCGAATT CGCCTGCTCC GAAGCGGCGT CCGAGCTGAT CTACAACGCG CGGCAGGCGA TGCACCGGAT CCTGCACGGC ATGGACGACC GTCTCATCGT CGTGATCGGG CCGTGCTCGA TCCACGACAC GAAGGCGGCG CTCGAATACG CGGGCCGGCT CGTGAAGGAA CGCGAGCGCT TCAAGGGCGA ACTCGAAATC GTGATGCGCG TGTACTTCGA GAAGCCGCGC ACGACGGTCG GCTGGAAGGG GCTCATCAAC GATCCGCATC TCGACAACAG CTTCAAGATC AACGAAGGGC TGCGCACCGC GCGCGAACTG CTGCTGCAGA TCAACGAGAT GGGGCTGCCG GCCGGCACCG AATACCTCGA CATGATCAGC CCGCAGTACA TCGCCGACCT GATCTCGTGG GGCGCGATCG GCGCGCGCAC GACCGAATCG CAGGTGCACC GCGAGCTGGC GTCGGGGCTG TCGTGCCCGG TCGGCTTCAA GAACGGCACC GACGGCAACG TGAAGATCGC GGTCGATGCG ATCAAGGCCG CATCGCAGCC GCACCACTTC CTGTCGGTGA CGAAGGGCGG CCACTCGGCG ATCGTGTCGA CGGCCGGCAA CGAGGACTGC CACGTGATCC TGCGCGGCGG CAAGGCGCCG AACTACGACG CGGACAGCGT GAACGCCGCG TGCGCGGACA TCGGCAAGGC GGGCCTCGCC GCGCGCCTGA TGATCGACGC GAGCCACGCG AACAGCTCGA AGAAGCACGA GAACCAGATT CCGGTATGCG CGGACATCGG CCGCCAGGTC GCGGCCGGCG ACTCGCGCAT CGTCGGCGTG ATGATCGAGT CGCACCTCGT CGAAGGCCGT CAGGATCTGA AGGAAGGCTG CGAACTGACG TACGGCCAGA GCATCACCGA CGCCTGCATC AACTGGGAAG ACAGCGTGAA GGTGCTCGAA GGGCTGGCGG AATCGGTGAA GGCGCGCCGC GTCGCGAGCG GTAGCGGAAA C
Details for BumuA.00090.a.B1.GE39676
HARVESTED ON: 12/3/2015
SEQUENCED ON: 12/7/2015
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMP PHNTDDVRIR ELKELTPPAH LIREFACSEA ASELIYNARQ AMHRILHGMD DRLIVVIGPC SIHDTKAALE YAGRLVKERE RFKGELEIVM RVYFEKPRTT VGWKGLINDP HLDNSFKINE GLRTARELLL QINEMGLPAG TEYLDMISPQ YIADLISWGA IGARTTESQV HRELASGLSC PVGFKNGTDG NVKIAVDAIK AASQPHHFLS VTKGGHSAIV STAGNEDCHV ILRGGKAPNY DADSVNAACA DIGKAGLAAR LMIDASHANS SKKHENQIPV CADIGRQVAA GDSRIVGVMI ESHLVEGRQD LKEGCELTYG QSITDACINW EDSVKVLEGL AESVKA
Validated NT Sequence
cgcgccttca ccgattccgc cagcccttcg agcaccttca cgctgtcttc ccagttgatg caggcgtcgg tgatgctctg gccgtacgtc agttcgcagc cttccttcag atcctgacgg ccttcgacga ggtgcgactc gatcatcacg ccgacgatgc gcgagtcgcc ggccgcgacc tggcggccga tgtccgcgca taccggaatc tggttctcgt gcttcttcga gctgttcgcg tggctcgcgt cgatcatcag gcgcgcggcg aggcccgcct tgccgatgtc cgcgcacgcg gcgttcacgc tgtccgcgtc gtagttcggc gccttgccgc cgcgcaggat cacgtggcag tcctcgttgc cggccgtcga cacgatcgcc gagtggccgc ccttcgtcac cgacaggaag tggtgcggct gcgatgcggc cttgatcgca tcgaccgcga tcttcacgtt gccgtcggtg ccgttcttga agccgaccgg gcacgacagc cccgacgcca gctcgcggtg cacctgcgat tcggtcgtgc gcgcgccgat cgcgccccac gagatcaggt cggcgatgta ctgcgggctg atcatgtcga ggtattcggt gccggccggc agccccatct cgttgatctg cagcagcagt tcgcgcgcgg tgcgcagccc ttcgttgatc ttgaagctgt tgtcgagatg cggatcgttg atgagcccct tccagccgac cgtcgtgcgc ggcttctcga agtacacgcg catcacgatt tcgagttcgc ccttgaagcg ctcgcgttcc ttcacgagcc ggcccgcgta ttcgagcgcc gccttcgtgt cgtggatcga gcacggcccg atcacgacga tgagacggtc gtccatgccg tgcaggatcc ggtgcatcgc ctgccgcgcg ttgtagatca gctcggacgc cgcttcggag caggcgaatt cgcggatcag gtgagcgggc ggcgtcagtt ccttgagttc gcggatgcgg acgtcgtcgg tattgtgcgg gggcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMP PHNTDDVRIR ELKELTPPAH LIREFACSEA ASELIYNARQ AMHRILHGMD DRLIVVIGPC SIHDTKAALE YAGRLVKERE RFKGELEIVM RVYFEKPRTT VGWKGLINDP HLDNSFKINE GLRTARELLL QINEMGLPAG TEYLDMISPQ YIADLISWGA IGARTTESQV HRELASGLSC PVGFKNGTDG NVKIAVDAIK AASQPHHFLS VTKGGHSAIV STAGNEDCHV ILRGGKAPNY DADSVNAACA DIGKAGLAAR LMIDASHANS SKKHENQIPV CADIGRQVAA GDSRIVGVMI ESHLVEGRQD LKEGCELTYG QSITDACINW EDSVKVLEGL AESVKARRVA SGSGN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccc cgcacaatac cgacgacgtc cgcatccgcg aactcaagga actgacgccg cccgctcacc tgatccgcga attcgcctgc tccgaagcgg cgtccgagct gatctacaac gcgcggcagg cgatgcaccg gatcctgcac ggcatggacg accgtctcat cgtcgtgatc gggccgtgct cgatccacga cacgaaggcg gcgctcgaat acgcgggccg gctcgtgaag gaacgcgagc gcttcaaggg cgaactcgaa atcgtgatgc gcgtgtactt cgagaagccg cgcacgacgg tcggctggaa ggggctcatc aacgatccgc atctcgacaa cagcttcaag atcaacgaag ggctgcgcac cgcgcgcgaa ctgctgctgc agatcaacga gatggggctg ccggccggca ccgaatacct cgacatgatc agcccgcagt acatcgccga cctgatctcg tggggcgcga tcggcgcgcg cacgaccgaa tcgcaggtgc accgcgagct ggcgtcgggg ctgtcgtgcc cggtcggctt caagaacggc accgacggca acgtgaagat cgcggtcgat gcgatcaagg ccgcatcgca gccgcaccac ttcctgtcgg tgacgaaggg cggccactcg gcgatcgtgt cgacggccgg caacgaggac tgccacgtga tcctgcgcgg cggcaaggcg ccgaactacg acgcggacag cgtgaacgcc gcgtgcgcgg acatcggcaa ggcgggcctc gccgcgcgcc tgatgatcga cgcgagccac gcgaacagct cgaagaagca cgagaaccag attccggtat gcgcggacat cggccgccag gtcgcggccg gcgactcgcg catcgtcggc gtgatgatcg agtcgcacct cgtcgaaggc cgtcaggatc tgaaggaagg ctgcgaactg acgtacggcc agagcatcac cgacgcctgc atcaactggg aagacagcgt gaaggtgctc gaagggctgg cggaatcggt gaaggcgcgc cgcgtcgcga gcggtagcgg aaactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BumuA.00090.a.B1.PS37904
PURIFICATION DATe: 4/4/2016
CONCENTRATION: 21.79mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP PHNTDDVRIR ELKELTPPAH LIREFACSEA ASELIYNARQ AMHRILHGMD DRLIVVIGPC SIHDTKAALE YAGRLVKERE RFKGELEIVM RVYFEKPRTT VGWKGLINDP HLDNSFKINE GLRTARELLL QINEMGLPAG TEYLDMISPQ YIADLISWGA IGARTTESQV HRELASGLSC PVGFKNGTDG NVKIAVDAIK AASQPHHFLS VTKGGHSAIV STAGNEDCHV ILRGGKAPNY DADSVNAACA DIGKAGLAAR LMIDASHANS SKKHENQIPV CADIGRQVAA GDSRIVGVMI ESHLVEGRQD LKEGCELTYG QSITDACINW EDSVKVLEGL AESVKA
Validated NT Sequence
cgcgccttca ccgattccgc cagcccttcg agcaccttca cgctgtcttc ccagttgatg caggcgtcgg tgatgctctg gccgtacgtc agttcgcagc cttccttcag atcctgacgg ccttcgacga ggtgcgactc gatcatcacg ccgacgatgc gcgagtcgcc ggccgcgacc tggcggccga tgtccgcgca taccggaatc tggttctcgt gcttcttcga gctgttcgcg tggctcgcgt cgatcatcag gcgcgcggcg aggcccgcct tgccgatgtc cgcgcacgcg gcgttcacgc tgtccgcgtc gtagttcggc gccttgccgc cgcgcaggat cacgtggcag tcctcgttgc cggccgtcga cacgatcgcc gagtggccgc ccttcgtcac cgacaggaag tggtgcggct gcgatgcggc cttgatcgca tcgaccgcga tcttcacgtt gccgtcggtg ccgttcttga agccgaccgg gcacgacagc cccgacgcca gctcgcggtg cacctgcgat tcggtcgtgc gcgcgccgat cgcgccccac gagatcaggt cggcgatgta ctgcgggctg atcatgtcga ggtattcggt gccggccggc agccccatct cgttgatctg cagcagcagt tcgcgcgcgg tgcgcagccc ttcgttgatc ttgaagctgt tgtcgagatg cggatcgttg atgagcccct tccagccgac cgtcgtgcgc ggcttctcga agtacacgcg catcacgatt tcgagttcgc ccttgaagcg ctcgcgttcc ttcacgagcc ggcccgcgta ttcgagcgcc gccttcgtgt cgtggatcga gcacggcccg atcacgacga tgagacggtc gtccatgccg tgcaggatcc ggtgcatcgc ctgccgcgcg ttgtagatca gctcggacgc cgcttcggag caggcgaatt cgcggatcag gtgagcgggc ggcgtcagtt ccttgagttc gcggatgcgg acgtcgtcgg tattgtgcgg gggcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMP PHNTDDVRIR ELKELTPPAH LIREFACSEA ASELIYNARQ AMHRILHGMD DRLIVVIGPC SIHDTKAALE YAGRLVKERE RFKGELEIVM RVYFEKPRTT VGWKGLINDP HLDNSFKINE GLRTARELLL QINEMGLPAG TEYLDMISPQ YIADLISWGA IGARTTESQV HRELASGLSC PVGFKNGTDG NVKIAVDAIK AASQPHHFLS VTKGGHSAIV STAGNEDCHV ILRGGKAPNY DADSVNAACA DIGKAGLAAR LMIDASHANS SKKHENQIPV CADIGRQVAA GDSRIVGVMI ESHLVEGRQD LKEGCELTYG QSITDACINW EDSVKVLEGL AESVKARRVA SGSGN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCCC CGCACAATAC CGACGACGTC CGCATCCGCG AACTCAAGGA ACTGACGCCG CCCGCTCACC TGATCCGCGA ATTCGCCTGC TCCGAAGCGG CGTCCGAGCT GATCTACAAC GCGCGGCAGG CGATGCACCG GATCCTGCAC GGCATGGACG ACCGTCTCAT CGTCGTGATC GGGCCGTGCT CGATCCACGA CACGAAGGCG GCGCTCGAAT ACGCGGGCCG GCTCGTGAAG GAACGCGAGC GCTTCAAGGG CGAACTCGAA ATCGTGATGC GCGTGTACTT CGAGAAGCCG CGCACGACGG TCGGCTGGAA GGGGCTCATC AACGATCCGC ATCTCGACAA CAGCTTCAAG ATCAACGAAG GGCTGCGCAC CGCGCGCGAA CTGCTGCTGC AGATCAACGA GATGGGGCTG CCGGCCGGCA CCGAATACCT CGACATGATC AGCCCGCAGT ACATCGCCGA CCTGATCTCG TGGGGCGCGA TCGGCGCGCG CACGACCGAA TCGCAGGTGC ACCGCGAGCT GGCGTCGGGG CTGTCGTGCC CGGTCGGCTT CAAGAACGGC ACCGACGGCA ACGTGAAGAT CGCGGTCGAT GCGATCAAGG CCGCATCGCA GCCGCACCAC TTCCTGTCGG TGACGAAGGG CGGCCACTCG GCGATCGTGT CGACGGCCGG CAACGAGGAC TGCCACGTGA TCCTGCGCGG CGGCAAGGCG CCGAACTACG ACGCGGACAG CGTGAACGCC GCGTGCGCGG ACATCGGCAA GGCGGGCCTC GCCGCGCGCC TGATGATCGA CGCGAGCCAC GCGAACAGCT CGAAGAAGCA CGAGAACCAG ATTCCGGTAT GCGCGGACAT CGGCCGCCAG GTCGCGGCCG GCGACTCGCG CATCGTCGGC GTGATGATCG AGTCGCACCT CGTCGAAGGC CGTCAGGATC TGAAGGAAGG CTGCGAACTG ACGTACGGCC AGAGCATCAC CGACGCCTGC ATCAACTGGG AAGACAGCGT GAAGGTGCTC GAAGGGCTGG CGGAATCGGT GAAGGCGCGC CGCGTCGCGA GCGGTAGCGG AAACTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT