SSGCID: Target Page

BumuA.00088.a

Ornithine carbamoyltransferase

CENTER ID:	BumuA.00088.a
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00088.a.A1.GE32153	Full length( BumuA.00088.a )		1	309

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00088.a.A1.PW32071	Full length( BumuA.00088.a )		1	309

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.894
RefSeq:	YP_001578935.1
UniProt:	A0A0H3KH53

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTAKTIRHYL QFKDFSLEDY EYVLERTGIL KRKFKNYETY HPLHDRTLAM IFEKSSTRTR LSFEAGIFQL GGHAVFMSTR DTQLGRGEPV EDSAQVISRM VDIIMIRTFE QDIIQRFAEN SRVPVINGLT NEYHPCQVLA DIFTYYEHRG PIAGKTVAWV GDANNMLYTW IQAAQILGFK LRLSTPPGYA LDMKLVSPDS APFYEVFDDP NEACKGADLV TTDVWTSMGF EAENEARMKA FADWCVDEEM MGHANPDALF MHCLPAHRGE EVTAGVIDGP QSVVWDEAEN RLHVQKALME FLLLGRLKH

NT Sequence

ATGACCGCCA AAACCATTCG TCACTACCTG CAGTTCAAGG ATTTCTCGCT GGAAGACTAC GAGTACGTGC TCGAACGCAC GGGTATCCTG AAGCGCAAGT TCAAGAACTA CGAGACCTAC CATCCGCTGC ACGACCGCAC GCTCGCGATG ATCTTCGAGA AGAGCTCGAC GCGCACGCGT CTGTCGTTCG AAGCGGGGAT CTTCCAGCTC GGCGGCCACG CGGTATTCAT GAGCACGCGC GACACGCAGC TCGGCCGCGG CGAGCCGGTC GAGGATTCCG CGCAGGTCAT CTCGCGGATG GTCGACATCA TCATGATCCG CACGTTCGAG CAGGACATCA TCCAGCGCTT CGCGGAAAAC TCGCGCGTGC CGGTGATCAA CGGGCTGACC AACGAATACC ACCCGTGCCA GGTGCTCGCC GACATCTTCA CGTACTACGA GCACCGCGGC CCGATCGCGG GCAAGACCGT CGCATGGGTC GGCGACGCGA ACAACATGCT CTATACGTGG ATTCAGGCCG CGCAGATCCT CGGCTTCAAG CTGCGCCTGT CGACGCCGCC CGGCTACGCG CTCGACATGA AGCTCGTGTC GCCCGACAGC GCACCGTTCT ACGAGGTGTT CGACGATCCG AACGAAGCAT GCAAGGGTGC CGATCTCGTC ACGACCGATG TCTGGACGAG CATGGGTTTC GAGGCCGAGA ACGAGGCACG GATGAAGGCG TTTGCCGACT GGTGCGTCGA CGAGGAAATG ATGGGCCATG CGAATCCGGA CGCGCTGTTC ATGCACTGCC TGCCCGCGCA TCGCGGCGAG GAAGTGACGG CCGGCGTGAT CGACGGCCCG CAAAGCGTCG TGTGGGACGA AGCGGAAAAC CGGCTGCACG TGCAGAAGGC GCTGATGGAG TTCCTGCTGC TCGGCCGCCT CAAGCAC

Details for BumuA.00088.a.A1.GE32153

HARVESTED ON:	4/26/2011
SEQUENCED ON:	5/2/2011
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMTAKTIR HYLQFKDFSL EDYEYVLERT GILKRKFKNY ETYHPLHDRT LAMIFEKSST RTRLSFEAGI FQLGGHAVFM STRDTQLGRG EPVEDSAQVI SRMVDIIMIR TFEQDIIQRF AENSRVPVIN GLTNEYHPCQ VLADIFTYYE HRGPIAGKTV AWVGDANNML YTWIQAAQIL GFKLRLSTPP GYALDMKLVS PDSAPFYEVF DDPNEACKGA DLVTTDVWTS MGFEAENEAR MKAFADWCVD EEMMGHANPD ALFMHCLPAH RGEEVTAGVI DGPQSVVWDE AENRLHVQKA LMEFLLLGRL

Validated NT Sequence

tgaggcggcc gagcagcagg aactccatca gcgccttctg cacgtgcagc cggttttccg cttcgtccca cacgacgctt tgcgggccgt cgatcacgcc ggccgtcact tcctcgccgc gatgcgcggg caggcagtgc atgaacagcg cgtccggatt cgcatggccc atcatttcct cgtcgacgca ccagtcggca aacgccttca tccgtgcctc gttctcggcc tcgaaaccca tgctcgtcca gacatcggtc gtgacgagat cggcaccctt gcatgcttcg ttcggatcgt cgaacacctc gtagaacggt gcgctgtcgg gcgacacgag cttcatgtcg agcgcgtagc cgggcggcgt cgacaggcgc agcttgaagc cgaggatctg cgcggcctga atccacgtat agagcatgtt gttcgcgtcg ccgacccatg cgacggtctt gcccgcgatc gggccgcggt gctcgtagta cgtgaagatg tcggcgagca cctggcacgg gtggtattcg ttggtcagcc cgttgatcac cggcacgcgc gagttttccg cgaagcgctg gatgatgtcc tgctcgaacg tgcggatcat gatgatgtcg accatccgcg agatgacctg cgcggaatcc tcgaccggct cgccgcggcc gagctgcgtg tcgcgcgtgc tcatgaatac cgcgtggccg ccgagctgga agatccccgc ttcgaacgac agacgcgtgc gcgtcgagct cttctcgaag atcatcgcga gcgtgcggtc gtgcagcgga tggtaggtct cgtagttctt gaacttgcgc ttcaggatac ccgtgcgttc gagcacgtac tcgtagtctt ccagcgagaa atccttgaac tgcaggtagt gacgaatggt tttggcggtc atcgaaccag gaccctgggt ctgagcttcc agggtaccca ta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTAKTIRHY LQFKDFSLED YEYVLERTGI LKRKFKNYET YHPLHDRTLA MIFEKSSTRT RLSFEAGIFQ LGGHAVFMST RDTQLGRGEP VEDSAQVISR MVDIIMIRTF EQDIIQRFAE NSRVPVINGL TNEYHPCQVL ADIFTYYEHR GPIAGKTVAW VGDANNMLYT WIQAAQILGF KLRLSTPPGY ALDMKLVSPD SAPFYEVFDD PNEACKGADL VTTDVWTSMG FEAENEARMK AFADWCVDEE MMGHANPDAL FMHCLPAHRG EEVTAGVIDG PQSVVWDEAE NRLHVQKALM EFLLLGRLKH

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgccaaa accattcgtc actacctgca gttcaaggat ttctcgctgg aagactacga gtacgtgctc gaacgcacgg gtatcctgaa gcgcaagttc aagaactacg agacctacca tccgctgcac gaccgcacgc tcgcgatgat cttcgagaag agctcgacgc gcacgcgtct gtcgttcgaa gcggggatct tccagctcgg cggccacgcg gtattcatga gcacgcgcga cacgcagctc ggccgcggcg agccggtcga ggattccgcg caggtcatct cgcggatggt cgacatcatc atgatccgca cgttcgagca ggacatcatc cagcgcttcg cggaaaactc gcgcgtgccg gtgatcaacg ggctgaccaa cgaataccac ccgtgccagg tgctcgccga catcttcacg tactacgagc accgcggccc gatcgcgggc aagaccgtcg catgggtcgg cgacgcgaac aacatgctct atacgtggat tcaggccgcg cagatcctcg gcttcaagct gcgcctgtcg acgccgcccg gctacgcgct cgacatgaag ctcgtgtcgc ccgacagcgc accgttctac gaggtgttcg acgatccgaa cgaagcatgc aagggtgccg atctcgtcac gaccgatgtc tggacgagca tgggtttcga ggccgagaac gaggcacgga tgaaggcgtt tgccgactgg tgcgtcgacg aggaaatgat gggccatgcg aatccggacg cgctgttcat gcactgcctg cccgcgcatc gcggcgagga agtgacggcc ggcgtgatcg acggcccgca aagcgtcgtg tgggacgaag cggaaaaccg gctgcacgtg cagaaggcgc tgatggagtt cctgctgctc ggccgcctca agcactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BumuA.00088.a.A1.PW32071

PURIFICATION DATe:	6/10/2011
CONCENTRATION:	31.49mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	34
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGCCAAA ACCATTCGTC ACTACCTGCA GTTCAAGGAT TTCTCGCTGG AAGACTACGA GTACGTGCTC GAACGCACGG GTATCCTGAA GCGCAAGTTC AAGAACTACG AGACCTACCA TCCGCTGCAC GACCGCACGC TCGCGATGAT CTTCGAGAAG AGCTCGACGC GCACGCGTCT GTCGTTCGAA GCGGGGATCT TCCAGCTCGG CGGCCACGCG GTATTCATGA GCACGCGCGA CACGCAGCTC GGCCGCGGCG AGCCGGTCGA GGATTCCGCG CAGGTCATCT CGCGGATGGT CGACATCATC ATGATCCGCA CGTTCGAGCA GGACATCATC CAGCGCTTCG CGGAAAACTC GCGCGTGCCG GTGATCAACG GGCTGACCAA CGAATACCAC CCGTGCCAGG TGCTCGCCGA CATCTTCACG TACTACGAGC ACCGCGGCCC GATCGCGGGC AAGACCGTCG CATGGGTCGG CGACGCGAAC AACATGCTCT ATACGTGGAT TCAGGCCGCG CAGATCCTCG GCTTCAAGCT GCGCCTGTCG ACGCCGCCCG GCTACGCGCT CGACATGAAG CTCGTGTCGC CCGACAGCGC ACCGTTCTAC GAGGTGTTCG ACGATCCGAA CGAAGCATGC AAGGGTGCCG ATCTCGTCAC GACCGATGTC TGGACGAGCA TGGGTTTCGA GGCCGAGAAC GAGGCACGGA TGAAGGCGTT TGCCGACTGG TGCGTCGACG AGGAAATGAT GGGCCATGCG AATCCGGACG CGCTGTTCAT GCACTGCCTG CCCGCGCATC GCGGCGAGGA AGTGACGGCC GGCGTGATCG ACGGCCCGCA AAGCGTCGTG TGGGACGAAG CGGAAAACCG GCTGCACGTG CAGAAGGCGC TGATGGAGTT CCTGCTGCTC GGCCGCCTCA AGCACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA