BumuA.00082.a

Porphobilinogen deaminase

CENTER ID: BumuA.00082.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00082.a.A1.GE33095 Full length( BumuA.00082.a ) 1 402
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00082.a.A1.PS01263 Full length( BumuA.00082.a ) 1 402

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.1026
RefSeq: YP_001579060.1
UniProt: A9AHL0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGFLGKARVK RNCGNCENCG QATSYVRHIR ASAPQAARGR GGRGRPARAG RSVRATIVRS SIPSFDVPAM NSEILAAGPR PAQPPATLTI ASRESRLAMW QAEHVRDALR KLYPACDVKI LGMTTRGDQI LDRTLSKVGG KGLFVKELES ALADGRADLA VHSLKDVPMM LPDGFALAAI MQREDPRDAF VSNDYASLDA LPAGAVVGTS SLRREAMLRA RYPQLQVLPL RGNLDTRLSK LDRGDYAAII LAAAGLKRLG LEARIRALLD VEDSPPAAGQ GALGIEIAAH RTDVAAWLEP LHDAQTALAV EAERMVSRAL GGSCEVPLAA HAVWRAGELY LTGRVSTIDG TRVLTAEECA SVVTVADALA LGRAVSDELE AQGALEIVRA LLAGSQAGKG EA
NT Sequence
ATGGGTTTCC TTGGGAAAGC TCGAGTCAAA AGGAACTGCG GAAACTGCGA GAACTGTGGT CAAGCAACCA GCTACGTACG GCACATCCGC GCATCCGCGC CGCAGGCCGC GCGAGGGCGC GGCGGCCGCG GGCGCCCGGC CCGGGCGGGC CGAAGCGTGC GTGCTACCAT TGTTCGGTCT TCAATCCCGT CCTTCGATGT TCCTGCAATG AATTCCGAGA TTCTCGCGGC CGGGCCGCGA CCGGCACAGC CGCCGGCGAC GCTGACGATC GCTTCGCGCG AAAGCCGCCT GGCGATGTGG CAAGCCGAAC ATGTGCGTGA TGCGCTGCGC AAATTATATC CAGCTTGTGA CGTGAAAATC CTCGGGATGA CGACGCGCGG CGATCAGATT CTCGATCGCA CGCTGTCGAA GGTCGGCGGC AAGGGACTCT TCGTCAAGGA GCTCGAGAGC GCGCTCGCCG ACGGCCGCGC CGATCTCGCC GTGCACTCGC TGAAGGACGT GCCGATGATG CTGCCGGACG GCTTCGCGCT CGCCGCGATC ATGCAGCGCG AGGATCCGCG CGACGCGTTC GTGTCGAACG ACTACGCGTC GCTCGACGCG CTGCCGGCCG GCGCGGTCGT CGGCACGTCG AGCCTGCGCC GCGAAGCGAT GCTGCGCGCG CGTTATCCGC AGCTGCAGGT GCTGCCGCTG CGCGGCAATC TCGATACGCG GCTCTCGAAG CTCGATCGCG GCGATTACGC GGCGATCATT CTCGCCGCCG CGGGCCTGAA GCGGCTCGGT CTGGAGGCGC GGATCCGCGC GCTGCTCGAC GTCGAGGACA GCCCGCCCGC AGCCGGTCAG GGTGCGCTCG GCATCGAGAT CGCCGCACAC CGCACGGACG TCGCCGCATG GCTCGAACCG CTGCACGACG CGCAGACGGC GCTCGCCGTC GAGGCCGAGC GGATGGTGTC GCGCGCGCTC GGCGGCAGCT GCGAGGTGCC GCTCGCCGCG CACGCCGTGT GGCGGGCCGG CGAGCTCTAT CTGACGGGCC GCGTGTCGAC GATCGACGGC ACGCGCGTGC TGACGGCCGA GGAGTGCGCG TCCGTCGTGA CGGTCGCCGA TGCGCTCGCG CTCGGCCGCG CGGTTTCCGA CGAGCTCGAG GCGCAGGGCG CGCTCGAGAT CGTCCGCGCA CTGCTCGCCG GCTCGCAGGC CGGCAAGGGC GAGGCC
Details for BumuA.00082.a.A1.GE33095
HARVESTED ON: 8/19/2011
SEQUENCED ON: 8/31/2011
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MGFLGKARVK RNCGNCENCG QATSYVRHIR ASAPQAARGR GGRGRPARAG RSVRATIVRS SIPSFDVPAM NSEILAAGPR PAQPPATLTI ASRESRLAMW QAEHVRDALR KLYPACDVKI LGMTTRGDQI LDRTLSKVGG KGLFVKELES ALADGRADLA VHSLKDVPMM LPDGFALAAI MQREDPRDAF VSNDYASLDA LPAGAVVGTS SLRREAMLRA RYPQLQVLPL RGNLDTRLSK LDRGDYAAII LAAAGLKRLG LEARIRALLD VEDSPPAAGQ GALGIEIAAH RTDVAAWLEP LHDAQTALAV EAERMVSRAL GGSCEVPLAA HAVWRAGELY LTGRVSTIDG TRVLTAEECA SVVTVADALA LGRAVSDELE AQGALEIVRA LLAGSQAGKG
Validated NT Sequence
cgcccttgcc ggcctgcgag ccggcgagca gtgcgcggac gatctcgagc gcgccctgcg cctcgagctc gtcggaaacc gcgcggccga gcgcgagcgc atcggcgacc gtcacgacgg acgcgcactc ctcggccgtc agcacgcgcg tgccgtcgat cgtcgacacg cggcccgtca gatagagctc gccggcccgc cacacggcgt gcgcggcgag cggcacctcg cagctgccgc cgagcgcgcg cgacaccatc cgctcggcct cgacggcgag cgccgtctgc gcgtcgtgca gcggttcgag ccatgcggcg acgtccgtgc ggtgtgcggc gatctcgatg ccgagcgcac cctgaccggc tgcgggcggg ctgtcctcga cgtcgagcag cgcgcggatc cgcgcctcca gaccgagccg cttcaggccc gcggcggcga gaatgatcgc cgcgtaatcg ccgcgatcga gcttcgagag ccgcgtatcg agattgccgc gcagcggcag cacctgcagc tgcggataac gcgcgcgcag catcgcttcg cggcgcaggc tcgacgtgcc gacgaccgcg ccggccggca gcgcgtcgag cgacgcgtag tcgttcgaca cgaacgcgtc gcgcggatcc tcgcgctgca tgatcgcggc gagcgcgaag ccgtccggca gcatcatcgg cacgtccttc agcgagtgca cggcgagatc ggcgcggccg tcggcgagcg cgctctcgag ctccttgacg aagagtccct tgccgccgac cttcgacagc gtgcgatcga gaatctgatc gccgcgcgtc gtcatcccga ggattttcac gtcacaagct ggatataatt tgcgcagcgc atcacgcaca tgttcggctt gccacatcgc caggcggctt tcgcgcgaag cgatcgtcag cgtcgccggc ggctgtgccg gtcgcggccc ggccgcgaga atctcggaat tcattgcagg aacatcgaag gacgggattg aagaccgaac aatggtagca cgcacgcttc ggcccgcccg ggccgggcgc ccgcggccgc cgcgccctcg cgcggcctgc ggcgcggatg cgcggatgtg ccgtacgtag ctggttgctt gaccacagtt ctcgcagttt ccgcagttcc ttttgactcg agctttccca aggaaaccca tcgaaccagg accctgggtc tgagcttcca ggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGFLGKARV KRNCGNCENC GQATSYVRHI RASAPQAARG RGGRGRPARA GRSVRATIVR SSIPSFDVPA MNSEILAAGP RPAQPPATLT IASRESRLAM WQAEHVRDAL RKLYPACDVK ILGMTTRGDQ ILDRTLSKVG GKGLFVKELE SALADGRADL AVHSLKDVPM MLPDGFALAA IMQREDPRDA FVSNDYASLD ALPAGAVVGT SSLRREAMLR ARYPQLQVLP LRGNLDTRLS KLDRGDYAAI ILAAAGLKRL GLEARIRALL DVEDSPPAAG QGALGIEIAA HRTDVAAWLE PLHDAQTALA VEAERMVSRA LGGSCEVPLA AHAVWRAGEL YLTGRVSTID GTRVLTAEEC ASVVTVADAL ALGRAVSDEL EAQGALEIVR ALLAGSQAGK GEA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtttcctt gggaaagctc gagtcaaaag gaactgcgga aactgcgaga actgtggtca agcaaccagc tacgtacggc acatccgcgc atccgcgccg caggccgcgc gagggcgcgg cggccgcggg cgcccggccc gggcgggccg aagcgtgcgt gctaccattg ttcggtcttc aatcccgtcc ttcgatgttc ctgcaatgaa ttccgagatt ctcgcggccg ggccgcgacc ggcacagccg ccggcgacgc tgacgatcgc ttcgcgcgaa agccgcctgg cgatgtggca agccgaacat gtgcgtgatg cgctgcgcaa attatatcca gcttgtgacg tgaaaatcct cgggatgacg acgcgcggcg atcagattct cgatcgcacg ctgtcgaagg tcggcggcaa gggactcttc gtcaaggagc tcgagagcgc gctcgccgac ggccgcgccg atctcgccgt gcactcgctg aaggacgtgc cgatgatgct gccggacggc ttcgcgctcg ccgcgatcat gcagcgcgag gatccgcgcg acgcgttcgt gtcgaacgac tacgcgtcgc tcgacgcgct gccggccggc gcggtcgtcg gcacgtcgag cctgcgccgc gaagcgatgc tgcgcgcgcg ttatccgcag ctgcaggtgc tgccgctgcg cggcaatctc gatacgcggc tctcgaagct cgatcgcggc gattacgcgg cgatcattct cgccgccgcg ggcctgaagc ggctcggtct ggaggcgcgg atccgcgcgc tgctcgacgt cgaggacagc ccgcccgcag ccggtcaggg tgcgctcggc atcgagatcg ccgcacaccg cacggacgtc gccgcatggc tcgaaccgct gcacgacgcg cagacggcgc tcgccgtcga ggccgagcgg atggtgtcgc gcgcgctcgg cggcagctgc gaggtgccgc tcgccgcgca cgccgtgtgg cgggccggcg agctctatct gacgggccgc gtgtcgacga tcgacggcac gcgcgtgctg acggccgagg agtgcgcgtc cgtcgtgacg gtcgccgatg cgctcgcgct cggccgcgcg gtttccgacg agctcgaggc gcagggcgcg ctcgagatcg tccgcgcact gctcgccggc tcgcaggccg gcaagggcga ggcctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BumuA.00082.a.A1.PS01263
PURIFICATION DATe: 11/15/2011
CONCENTRATION: 28.18mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MGFLGKARVK RNCGNCENCG QATSYVRHIR ASAPQAARGR GGRGRPARAG RSVRATIVRS SIPSFDVPAM NSEILAAGPR PAQPPATLTI ASRESRLAMW QAEHVRDALR KLYPACDVKI LGMTTRGDQI LDRTLSKVGG KGLFVKELES ALADGRADLA VHSLKDVPMM LPDGFALAAI MQREDPRDAF VSNDYASLDA LPAGAVVGTS SLRREAMLRA RYPQLQVLPL RGNLDTRLSK LDRGDYAAII LAAAGLKRLG LEARIRALLD VEDSPPAAGQ GALGIEIAAH RTDVAAWLEP LHDAQTALAV EAERMVSRAL GGSCEVPLAA HAVWRAGELY LTGRVSTIDG TRVLTAEECA SVVTVADALA LGRAVSDELE AQGALEIVRA LLAGSQAGKG
Validated NT Sequence
cgcccttgcc ggcctgcgag ccggcgagca gtgcgcggac gatctcgagc gcgccctgcg cctcgagctc gtcggaaacc gcgcggccga gcgcgagcgc atcggcgacc gtcacgacgg acgcgcactc ctcggccgtc agcacgcgcg tgccgtcgat cgtcgacacg cggcccgtca gatagagctc gccggcccgc cacacggcgt gcgcggcgag cggcacctcg cagctgccgc cgagcgcgcg cgacaccatc cgctcggcct cgacggcgag cgccgtctgc gcgtcgtgca gcggttcgag ccatgcggcg acgtccgtgc ggtgtgcggc gatctcgatg ccgagcgcac cctgaccggc tgcgggcggg ctgtcctcga cgtcgagcag cgcgcggatc cgcgcctcca gaccgagccg cttcaggccc gcggcggcga gaatgatcgc cgcgtaatcg ccgcgatcga gcttcgagag ccgcgtatcg agattgccgc gcagcggcag cacctgcagc tgcggataac gcgcgcgcag catcgcttcg cggcgcaggc tcgacgtgcc gacgaccgcg ccggccggca gcgcgtcgag cgacgcgtag tcgttcgaca cgaacgcgtc gcgcggatcc tcgcgctgca tgatcgcggc gagcgcgaag ccgtccggca gcatcatcgg cacgtccttc agcgagtgca cggcgagatc ggcgcggccg tcggcgagcg cgctctcgag ctccttgacg aagagtccct tgccgccgac cttcgacagc gtgcgatcga gaatctgatc gccgcgcgtc gtcatcccga ggattttcac gtcacaagct ggatataatt tgcgcagcgc atcacgcaca tgttcggctt gccacatcgc caggcggctt tcgcgcgaag cgatcgtcag cgtcgccggc ggctgtgccg gtcgcggccc ggccgcgaga atctcggaat tcattgcagg aacatcgaag gacgggattg aagaccgaac aatggtagca cgcacgcttc ggcccgcccg ggccgggcgc ccgcggccgc cgcgccctcg cgcggcctgc ggcgcggatg cgcggatgtg ccgtacgtag ctggttgctt gaccacagtt ctcgcagttt ccgcagttcc ttttgactcg agctttccca aggaaaccca tcgaaccagg accctgggtc tgagcttcca ggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGFLGKARV KRNCGNCENC GQATSYVRHI RASAPQAARG RGGRGRPARA GRSVRATIVR SSIPSFDVPA MNSEILAAGP RPAQPPATLT IASRESRLAM WQAEHVRDAL RKLYPACDVK ILGMTTRGDQ ILDRTLSKVG GKGLFVKELE SALADGRADL AVHSLKDVPM MLPDGFALAA IMQREDPRDA FVSNDYASLD ALPAGAVVGT SSLRREAMLR ARYPQLQVLP LRGNLDTRLS KLDRGDYAAI ILAAAGLKRL GLEARIRALL DVEDSPPAAG QGALGIEIAA HRTDVAAWLE PLHDAQTALA VEAERMVSRA LGGSCEVPLA AHAVWRAGEL YLTGRVSTID GTRVLTAEEC ASVVTVADAL ALGRAVSDEL EAQGALEIVR ALLAGSQAGK GEA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGTTTCCTT GGGAAAGCTC GAGTCAAAAG GAACTGCGGA AACTGCGAGA ACTGTGGTCA AGCAACCAGC TACGTACGGC ACATCCGCGC ATCCGCGCCG CAGGCCGCGC GAGGGCGCGG CGGCCGCGGG CGCCCGGCCC GGGCGGGCCG AAGCGTGCGT GCTACCATTG TTCGGTCTTC AATCCCGTCC TTCGATGTTC CTGCAATGAA TTCCGAGATT CTCGCGGCCG GGCCGCGACC GGCACAGCCG CCGGCGACGC TGACGATCGC TTCGCGCGAA AGCCGCCTGG CGATGTGGCA AGCCGAACAT GTGCGTGATG CGCTGCGCAA ATTATATCCA GCTTGTGACG TGAAAATCCT CGGGATGACG ACGCGCGGCG ATCAGATTCT CGATCGCACG CTGTCGAAGG TCGGCGGCAA GGGACTCTTC GTCAAGGAGC TCGAGAGCGC GCTCGCCGAC GGCCGCGCCG ATCTCGCCGT GCACTCGCTG AAGGACGTGC CGATGATGCT GCCGGACGGC TTCGCGCTCG CCGCGATCAT GCAGCGCGAG GATCCGCGCG ACGCGTTCGT GTCGAACGAC TACGCGTCGC TCGACGCGCT GCCGGCCGGC GCGGTCGTCG GCACGTCGAG CCTGCGCCGC GAAGCGATGC TGCGCGCGCG TTATCCGCAG CTGCAGGTGC TGCCGCTGCG CGGCAATCTC GATACGCGGC TCTCGAAGCT CGATCGCGGC GATTACGCGG CGATCATTCT CGCCGCCGCG GGCCTGAAGC GGCTCGGTCT GGAGGCGCGG ATCCGCGCGC TGCTCGACGT CGAGGACAGC CCGCCCGCAG CCGGTCAGGG TGCGCTCGGC ATCGAGATCG CCGCACACCG CACGGACGTC GCCGCATGGC TCGAACCGCT GCACGACGCG CAGACGGCGC TCGCCGTCGA GGCCGAGCGG ATGGTGTCGC GCGCGCTCGG CGGCAGCTGC GAGGTGCCGC TCGCCGCGCA CGCCGTGTGG CGGGCCGGCG AGCTCTATCT GACGGGCCGC GTGTCGACGA TCGACGGCAC GCGCGTGCTG ACGGCCGAGG AGTGCGCGTC CGTCGTGACG GTCGCCGATG CGCTCGCGCT CGGCCGCGCG GTTTCCGACG AGCTCGAGGC GCAGGGCGCG CTCGAGATCG TCCGCGCACT GCTCGCCGGC TCGCAGGCCG GCAAGGGCGA GGCCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA