BumuA.00081.a

Aminotransferase class V

CENTER ID: BumuA.00081.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00081.a.A1.GE33094 Full length( BumuA.00081.a ) 1 492
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00081.a.A1.PW33704 Full length( BumuA.00081.a ) 1 492

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.5527
RefSeq: YP_001585152.1
UniProt: A0A0H3KSU2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDSTPFHYL DYAATTPADP RVIEAMTTCL GFDGIFGNPA SSSHNAGRLA RQKVEHARKQ VAALIGAHAD EIVWTSGATE SNNLALKGYA DTATERRHLI TSRIEHKAIL DTMANLSKRG MTVTWLSPTA DGEITADAVA AAIGPDTGLV SLMLVNNELG TLTDVGAIAR IVHAAGALLH VDAAQALGKT PIDVHALGID MMSMSAHKVY GPKGIGALYV RRDIAERIAP QIHGGGHERG LRSGTLATHQ IVGMGVACEL AAAKLDSEAA RIAALATRLK DAVLALGDVT HNAAAAARIP HTLSLTVDAP GFLPFMLGDT LAVSSTSACN STSGAPSHVL TAIGLDAGAA SRTVRVSLGR FTTEQDIDAA IACFRRAIEQ CRAAASSGFA ASRQITPADL RAIRDAGYRA VICNRPDGED ADQPAFEEIA AAARELGLDA RYLPVGRDEI GDADVEAFAA LVDALPKPVL AYCRSGSRAG MLWHRLTMRS AA
NT Sequence
ATGAGCGACA GCACCCCGTT CCATTACCTC GACTACGCCG CCACCACGCC CGCCGATCCC CGCGTCATTG AAGCGATGAC CACCTGCCTC GGCTTCGACG GCATCTTCGG CAATCCGGCC TCCAGTTCGC ACAACGCAGG CCGCCTCGCA AGGCAGAAAG TCGAGCACGC GCGCAAACAG GTCGCCGCGC TGATCGGCGC ACACGCAGAC GAAATCGTCT GGACGTCGGG TGCCACCGAA TCGAACAACC TCGCGCTGAA GGGTTACGCG GACACCGCGA CGGAGCGGCG CCACCTGATC ACGAGCCGCA TCGAGCACAA GGCGATCCTC GACACGATGG CGAACCTGTC GAAGCGCGGG ATGACGGTCA CCTGGCTGTC GCCCACGGCG GACGGCGAAA TCACGGCCGA CGCCGTCGCC GCGGCGATCG GCCCCGATAC CGGTCTCGTC TCGCTGATGC TCGTGAACAA CGAACTCGGC ACGCTGACCG ACGTCGGCGC GATCGCGCGC ATCGTCCACG CGGCGGGCGC GCTGCTGCAC GTCGATGCCG CGCAGGCGCT CGGCAAGACG CCGATCGACG TGCACGCGCT CGGCATCGAC ATGATGTCGA TGTCCGCACA CAAGGTTTAC GGTCCCAAGG GTATCGGCGC GCTGTACGTG CGCCGCGACA TCGCCGAGCG GATCGCGCCG CAGATACATG GCGGCGGCCA CGAGCGCGGG CTGCGCTCGG GCACGCTCGC GACGCATCAG ATCGTCGGCA TGGGCGTCGC CTGCGAGCTC GCGGCAGCCA AGCTCGACAG CGAAGCCGCG CGCATTGCGG CGCTCGCCAC GCGCCTGAAG GATGCCGTGC TCGCACTCGG CGACGTCACG CACAACGCGG CCGCCGCCGC GCGCATTCCG CACACGCTGA GTCTGACGGT CGACGCACCG GGCTTCTTGC CGTTCATGCT GGGCGACACG CTGGCGGTGT CGTCGACGTC CGCATGCAAT TCGACGAGCG GCGCACCTTC GCATGTGCTG ACCGCGATCG GCCTCGATGC GGGCGCCGCG AGCCGCACCG TGCGCGTCAG CCTGGGCCGC TTCACGACCG AGCAGGACAT CGACGCCGCG ATCGCGTGCT TCCGGCGCGC GATCGAACAG TGCCGCGCCG CCGCATCGAG CGGCTTCGCC GCGTCGCGCC AGATCACGCC GGCCGACCTG CGCGCGATCC GCGACGCCGG CTATCGCGCC GTCATCTGCA ACCGGCCGGA CGGCGAAGAC GCCGATCAGC CCGCGTTCGA GGAAATCGCG GCAGCGGCAC GGGAACTGGG ACTCGATGCA CGCTATCTGC CGGTAGGTCG CGACGAAATC GGCGACGCGG ACGTCGAAGC GTTCGCCGCA CTCGTCGACG CGCTGCCGAA ACCGGTGCTC GCGTATTGCC GAAGCGGCAG CCGCGCCGGG ATGCTGTGGC ATCGACTGAC GATGCGCAGC GCCGCG
Details for BumuA.00081.a.A1.GE33094
HARVESTED ON: 8/19/2011
SEQUENCED ON: 8/31/2011
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 24
PERCENT COVERAGE: 96
Validated AA Sequence
MPQHPGAAAA SAIREHRFRQ RVDECGERFD VRVADFVATY RQIACIESQF PCRCRDFLER GLIGVFAVRP VADDGAIAGV ADRAQVGRRD LARRGEAARC GGAALFDRAP EARDRGVDVL LGREAAQADA HGAARGARIE ADRGQHMRRC AARRIACGRR RHRQRVAQHE RQEARCVDRQ TQRVRNARGG GRVVRDVAEC EHGILQARGE RRNARGFAVE LGCRELAGDA HADDLMRRER ARAQPALVAA AXYLRRDPLG DVAAHVQRAD TLGTVNLVCG HRHHVDAERV HVDRRLAERL RGIDVQQRAR RVDDARDRAD VGQRAEFVVH EHQRDETGIG ADRRGDGVGR DFAVRRGRQP GDRHPALRQV RHRVEDRLVL DAARDQVAPL RRGVRVTLQR EVVRFGGTRR PDDFVCVCAD QRGDLFARVL DFLPCEAACV VRTGGRIAED AVEAEAGGHR FNDAGIGGRG GGVVEVMERG AVAHRTRTLG LSF
Validated NT Sequence
attacgcggc gctgcgcatc gtcagtcgat gccacagcat cccggcgcgg ctgccgcttc ggcaatacgc gagcaccggt ttcggcagcg cgtcgacgag tgcggcgaac gcttcgacgt ccgcgtcgcc gatttcgtcg cgacctaccg gcagatagcg tgcatcgagt cccagttccc gtgccgctgc cgcgatttcc tcgaacgcgg gctgatcggc gtcttcgccg tccggccggt tgcagatgac ggcgcgatag ccggcgtcgc ggatcgcgcg caggtcggcc ggcgtgatct ggcgcgacgc ggcgaagccg ctcgatgcgg cggcgcggca ctgttcgatc gcgcgccgga agcacgcgat cgcggcgtcg atgtcctgct cggtcgtgaa gcggcccagg ctgacgcgca cggtgcggct cgcggcgccc gcatcgaggc cgatcgcggt cagcacatgc gaaggtgcgc cgctcgtcga attgcatgcg gacgtcgacg acaccgccag cgtgtcgccc agcatgaacg gcaagaagcc cggtgcgtcg accgtcagac tcagcgtgtg cggaatgcgc gcggcggcgg ccgcgttgtg cgtgacgtcg ccgagtgcga gcacggcatc cttcaggcgc gtggcgagcg ccgcaatgcg cgcggcttcg ctgtcgagct tggctgccgc gagctcgcag gcgacgccca tgccgacgat ctgatgcgtc gcgagcgtgc ccgagcgcag cccgcgctcg tggccgccgc cntgtatctg cggcgcgatc cgctcggcga tgtcgcggcg cacgtacagc gcgccgatac ccttgggacc gtaaaccttg tgtgcggaca tcgacatcat gtcgatgccg agcgcgtgca cgtcgatcgg cgtcttgccg agcgcctgcg cggcatcgac gtgcagcagc gcgcccgccg cgtggacgat gcgcgcgatc gcgccgacgt cggtcagcgt gccgagttcg ttgttcacga gcatcagcga gacgagaccg gtatcggggc cgatcgccgc ggcgacggcg tcggccgtga tttcgccgtc cgccgtgggc gacagccagg tgaccgtcat cccgcgcttc gacaggttcg ccatcgtgtc gaggatcgcc ttgtgctcga tgcggctcgt gatcaggtgg cgccgctccg tcgcggtgtc cgcgtaaccc ttcagcgcga ggttgttcga ttcggtggca cccgacgtcc agacgatttc gtctgcgtgt gcgccgatca gcgcggcgac ctgtttgcgc gcgtgctcga ctttctgcct tgcgaggcgg cctgcgttgt gcgaactgga ggccggattg ccgaagatgc cgtcgaagcc gaggcaggtg gtcatcgctt caatgacgcg gggatcggcg ggcgtggtgg cggcgtagtc gaggtaatgg aacggggtgc tgtcgctcat cgaaccagga ccctgggtct gagcttcca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDSTPFHY LDYAATTPAD PRVIEAMTTC LGFDGIFGNP ASSSHNAGRL ARQKVEHARK QVAALIGAHA DEIVWTSGAT ESNNLALKGY ADTATERRHL ITSRIEHKAI LDTMANLSKR GMTVTWLSPT ADGEITADAV AAAIGPDTGL VSLMLVNNEL GTLTDVGAIA RIVHAAGALL HVDAAQALGK TPIDVHALGI DMMSMSAHKV YGPKGIGALY VRRDIAERIA PQIHGGGHER GLRSGTLATH QIVGMGVACE LAAAKLDSEA ARIAALATRL KDAVLALGDV THNAAAAARI PHTLSLTVDA PGFLPFMLGD TLAVSSTSAC NSTSGAPSHV LTAIGLDAGA ASRTVRVSLG RFTTEQDIDA AIACFRRAIE QCRAAASSGF AASRQITPAD LRAIRDAGYR AVICNRPDGE DADQPAFEEI AAAARELGLD ARYLPVGRDE IGDADVEAFA ALVDALPKPV LAYCRSGSRA GMLWHRLTMR SAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgacagc accccgttcc attacctcga ctacgccgcc accacgcccg ccgatccccg cgtcattgaa gcgatgacca cctgcctcgg cttcgacggc atcttcggca atccggcctc cagttcgcac aacgcaggcc gcctcgcaag gcagaaagtc gagcacgcgc gcaaacaggt cgccgcgctg atcggcgcac acgcagacga aatcgtctgg acgtcgggtg ccaccgaatc gaacaacctc gcgctgaagg gttacgcgga caccgcgacg gagcggcgcc acctgatcac gagccgcatc gagcacaagg cgatcctcga cacgatggcg aacctgtcga agcgcgggat gacggtcacc tggctgtcgc ccacggcgga cggcgaaatc acggccgacg ccgtcgccgc ggcgatcggc cccgataccg gtctcgtctc gctgatgctc gtgaacaacg aactcggcac gctgaccgac gtcggcgcga tcgcgcgcat cgtccacgcg gcgggcgcgc tgctgcacgt cgatgccgcg caggcgctcg gcaagacgcc gatcgacgtg cacgcgctcg gcatcgacat gatgtcgatg tccgcacaca aggtttacgg tcccaagggt atcggcgcgc tgtacgtgcg ccgcgacatc gccgagcgga tcgcgccgca gatacatggc ggcggccacg agcgcgggct gcgctcgggc acgctcgcga cgcatcagat cgtcggcatg ggcgtcgcct gcgagctcgc ggcagccaag ctcgacagcg aagccgcgcg cattgcggcg ctcgccacgc gcctgaagga tgccgtgctc gcactcggcg acgtcacgca caacgcggcc gccgccgcgc gcattccgca cacgctgagt ctgacggtcg acgcaccggg cttcttgccg ttcatgctgg gcgacacgct ggcggtgtcg tcgacgtccg catgcaattc gacgagcggc gcaccttcgc atgtgctgac cgcgatcggc ctcgatgcgg gcgccgcgag ccgcaccgtg cgcgtcagcc tgggccgctt cacgaccgag caggacatcg acgccgcgat cgcgtgcttc cggcgcgcga tcgaacagtg ccgcgccgcc gcatcgagcg gcttcgccgc gtcgcgccag atcacgccgg ccgacctgcg cgcgatccgc gacgccggct atcgcgccgt catctgcaac cggccggacg gcgaagacgc cgatcagccc gcgttcgagg aaatcgcggc agcggcacgg gaactgggac tcgatgcacg ctatctgccg gtaggtcgcg acgaaatcgg cgacgcggac gtcgaagcgt tcgccgcact cgtcgacgcg ctgccgaaac cggtgctcgc gtattgccga agcggcagcc gcgccgggat gctgtggcat cgactgacga tgcgcagcgc cgcgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BumuA.00081.a.A1.PW33704
PURIFICATION DATe: 1/6/2012
CONCENTRATION: 8.56mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 24
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MPQHPGAAAA SAIREHRFRQ RVDECGERFD VRVADFVATY RQIACIESQF PCRCRDFLER GLIGVFAVRP VADDGAIAGV ADRAQVGRRD LARRGEAARC GGAALFDRAP EARDRGVDVL LGREAAQADA HGAARGARIE ADRGQHMRRC AARRIACGRR RHRQRVAQHE RQEARCVDRQ TQRVRNARGG GRVVRDVAEC EHGILQARGE RRNARGFAVE LGCRELAGDA HADDLMRRER ARAQPALVAA AXYLRRDPLG DVAAHVQRAD TLGTVNLVCG HRHHVDAERV HVDRRLAERL RGIDVQQRAR RVDDARDRAD VGQRAEFVVH EHQRDETGIG ADRRGDGVGR DFAVRRGRQP GDRHPALRQV RHRVEDRLVL DAARDQVAPL RRGVRVTLQR EVVRFGGTRR PDDFVCVCAD QRGDLFARVL DFLPCEAACV VRTGGRIAED AVEAEAGGHR FNDAGIGGRG GGVVEVMERG AVAHRTRTLG LSF
Validated NT Sequence
attacgcggc gctgcgcatc gtcagtcgat gccacagcat cccggcgcgg ctgccgcttc ggcaatacgc gagcaccggt ttcggcagcg cgtcgacgag tgcggcgaac gcttcgacgt ccgcgtcgcc gatttcgtcg cgacctaccg gcagatagcg tgcatcgagt cccagttccc gtgccgctgc cgcgatttcc tcgaacgcgg gctgatcggc gtcttcgccg tccggccggt tgcagatgac ggcgcgatag ccggcgtcgc ggatcgcgcg caggtcggcc ggcgtgatct ggcgcgacgc ggcgaagccg ctcgatgcgg cggcgcggca ctgttcgatc gcgcgccgga agcacgcgat cgcggcgtcg atgtcctgct cggtcgtgaa gcggcccagg ctgacgcgca cggtgcggct cgcggcgccc gcatcgaggc cgatcgcggt cagcacatgc gaaggtgcgc cgctcgtcga attgcatgcg gacgtcgacg acaccgccag cgtgtcgccc agcatgaacg gcaagaagcc cggtgcgtcg accgtcagac tcagcgtgtg cggaatgcgc gcggcggcgg ccgcgttgtg cgtgacgtcg ccgagtgcga gcacggcatc cttcaggcgc gtggcgagcg ccgcaatgcg cgcggcttcg ctgtcgagct tggctgccgc gagctcgcag gcgacgccca tgccgacgat ctgatgcgtc gcgagcgtgc ccgagcgcag cccgcgctcg tggccgccgc cntgtatctg cggcgcgatc cgctcggcga tgtcgcggcg cacgtacagc gcgccgatac ccttgggacc gtaaaccttg tgtgcggaca tcgacatcat gtcgatgccg agcgcgtgca cgtcgatcgg cgtcttgccg agcgcctgcg cggcatcgac gtgcagcagc gcgcccgccg cgtggacgat gcgcgcgatc gcgccgacgt cggtcagcgt gccgagttcg ttgttcacga gcatcagcga gacgagaccg gtatcggggc cgatcgccgc ggcgacggcg tcggccgtga tttcgccgtc cgccgtgggc gacagccagg tgaccgtcat cccgcgcttc gacaggttcg ccatcgtgtc gaggatcgcc ttgtgctcga tgcggctcgt gatcaggtgg cgccgctccg tcgcggtgtc cgcgtaaccc ttcagcgcga ggttgttcga ttcggtggca cccgacgtcc agacgatttc gtctgcgtgt gcgccgatca gcgcggcgac ctgtttgcgc gcgtgctcga ctttctgcct tgcgaggcgg cctgcgttgt gcgaactgga ggccggattg ccgaagatgc cgtcgaagcc gaggcaggtg gtcatcgctt caatgacgcg gggatcggcg ggcgtggtgg cggcgtagtc gaggtaatgg aacggggtgc tgtcgctcat cgaaccagga ccctgggtct gagcttcca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDSTPFHY LDYAATTPAD PRVIEAMTTC LGFDGIFGNP ASSSHNAGRL ARQKVEHARK QVAALIGAHA DEIVWTSGAT ESNNLALKGY ADTATERRHL ITSRIEHKAI LDTMANLSKR GMTVTWLSPT ADGEITADAV AAAIGPDTGL VSLMLVNNEL GTLTDVGAIA RIVHAAGALL HVDAAQALGK TPIDVHALGI DMMSMSAHKV YGPKGIGALY VRRDIAERIA PQIHGGGHER GLRSGTLATH QIVGMGVACE LAAAKLDSEA ARIAALATRL KDAVLALGDV THNAAAAARI PHTLSLTVDA PGFLPFMLGD TLAVSSTSAC NSTSGAPSHV LTAIGLDAGA ASRTVRVSLG RFTTEQDIDA AIACFRRAIE QCRAAASSGF AASRQITPAD LRAIRDAGYR AVICNRPDGE DADQPAFEEI AAAARELGLD ARYLPVGRDE IGDADVEAFA ALVDALPKPV LAYCRSGSRA GMLWHRLTMR SAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGACAGC ACCCCGTTCC ATTACCTCGA CTACGCCGCC ACCACGCCCG CCGATCCCCG CGTCATTGAA GCGATGACCA CCTGCCTCGG CTTCGACGGC ATCTTCGGCA ATCCGGCCTC CAGTTCGCAC AACGCAGGCC GCCTCGCAAG GCAGAAAGTC GAGCACGCGC GCAAACAGGT CGCCGCGCTG ATCGGCGCAC ACGCAGACGA AATCGTCTGG ACGTCGGGTG CCACCGAATC GAACAACCTC GCGCTGAAGG GTTACGCGGA CACCGCGACG GAGCGGCGCC ACCTGATCAC GAGCCGCATC GAGCACAAGG CGATCCTCGA CACGATGGCG AACCTGTCGA AGCGCGGGAT GACGGTCACC TGGCTGTCGC CCACGGCGGA CGGCGAAATC ACGGCCGACG CCGTCGCCGC GGCGATCGGC CCCGATACCG GTCTCGTCTC GCTGATGCTC GTGAACAACG AACTCGGCAC GCTGACCGAC GTCGGCGCGA TCGCGCGCAT CGTCCACGCG GCGGGCGCGC TGCTGCACGT CGATGCCGCG CAGGCGCTCG GCAAGACGCC GATCGACGTG CACGCGCTCG GCATCGACAT GATGTCGATG TCCGCACACA AGGTTTACGG TCCCAAGGGT ATCGGCGCGC TGTACGTGCG CCGCGACATC GCCGAGCGGA TCGCGCCGCA GATACATGGC GGCGGCCACG AGCGCGGGCT GCGCTCGGGC ACGCTCGCGA CGCATCAGAT CGTCGGCATG GGCGTCGCCT GCGAGCTCGC GGCAGCCAAG CTCGACAGCG AAGCCGCGCG CATTGCGGCG CTCGCCACGC GCCTGAAGGA TGCCGTGCTC GCACTCGGCG ACGTCACGCA CAACGCGGCC GCCGCCGCGC GCATTCCGCA CACGCTGAGT CTGACGGTCG ACGCACCGGG CTTCTTGCCG TTCATGCTGG GCGACACGCT GGCGGTGTCG TCGACGTCCG CATGCAATTC GACGAGCGGC GCACCTTCGC ATGTGCTGAC CGCGATCGGC CTCGATGCGG GCGCCGCGAG CCGCACCGTG CGCGTCAGCC TGGGCCGCTT CACGACCGAG CAGGACATCG ACGCCGCGAT CGCGTGCTTC CGGCGCGCGA TCGAACAGTG CCGCGCCGCC GCATCGAGCG GCTTCGCCGC GTCGCGCCAG ATCACGCCGG CCGACCTGCG CGCGATCCGC GACGCCGGCT ATCGCGCCGT CATCTGCAAC CGGCCGGACG GCGAAGACGC CGATCAGCCC GCGTTCGAGG AAATCGCGGC AGCGGCACGG GAACTGGGAC TCGATGCACG CTATCTGCCG GTAGGTCGCG ACGAAATCGG CGACGCGGAC GTCGAAGCGT TCGCCGCACT CGTCGACGCG CTGCCGAAAC CGGTGCTCGC GTATTGCCGA AGCGGCAGCC GCGCCGGGAT GCTGTGGCAT CGACTGACGA TGCGCAGCGC CGCGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA