BumuA.00078.a
Peptide deformylase
| CENTER ID: | BumuA.00078.a |
| ORGANISM: | Burkholderia multivorans ATCC 17616 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00078.a.B1.GE39674 | Full length( BumuA.00078.a ) | 1 | 181 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5J46
DEPOSITED: 3/31/2016
DETERMINATION: XRay
CLONE: BumuA.00078.a.B1.GE39674
PROTEIN: BumuA.00078.a.B1.PS37840
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128237 |
| PATRIC ID: | fig|395019.8.peg.3372 |
| RefSeq: | YP_001581305.1 |
| UniProt: | A0A0H3KB98 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANAAHRFTE YRKTMALLNI LHYPDKRLHK VAKPVDKVDD RIRKLVADMA ETMYAAPGIG LAATQVDVHE RVIVIDVSED KNELRAFINP EIIWSSDGKQ VYEEGCLSVP GIYDEVERPD RVRVRALNEQ GETFELDCEG LLAVCIQHEM DHLMGRVFVE YLSPLKQSRI KTKMKKLERA M
NT Sequence
ATGGCTAATG CGGCACACCG CTTCACCGAA TACCGAAAGA CCATGGCTTT GCTGAACATT CTTCATTACC CCGACAAGCG GCTGCACAAG GTCGCGAAGC CCGTCGACAA GGTCGACGAC CGCATCCGCA AGCTCGTTGC CGACATGGCC GAGACGATGT ACGCGGCGCC CGGCATCGGC CTCGCCGCGA CGCAGGTCGA CGTGCACGAA CGCGTGATCG TGATCGACGT GTCCGAAGAC AAGAACGAGC TGCGCGCCTT CATCAACCCC GAGATCATCT GGTCGAGCGA CGGCAAGCAG GTCTACGAAG AGGGCTGTCT GTCGGTGCCC GGCATCTACG ACGAAGTCGA GCGCCCGGAC CGCGTGCGCG TGCGCGCGCT CAACGAGCAG GGCGAGACGT TCGAGCTCGA CTGCGAAGGC CTGCTCGCCG TATGCATCCA GCACGAAATG GATCACCTGA TGGGCCGCGT GTTCGTCGAA TACCTGTCGC CGCTCAAACA GAGCCGGATC AAGACGAAGA TGAAGAAACT CGAACGCGCG ATG
Details for BumuA.00078.a.B1.GE39674
| HARVESTED ON: | 12/3/2015 |
| SEQUENCED ON: | 12/7/2015 |
| EXPECTED MW: | 22kDa |
| OBSERVED MW: | 22kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA NAAHRFTEYR KTMALLNILH YPDKRLHKVA KPVDKVDDRI RKLVADMAET MYAAPGIGLA ATQVDVHERV IVIDVSEDKN ELRAFINPEI IWSSDGKQVY EEGCLSVPGI YDEVERPDRV RVRALNEQGE TFELDCEGLL AVCIQHEMDH LMGRVFVEYL SPLKQSRIKT KMXXLERAM
Validated NT Sequence
atcttactca cttacatcgc gcgttcgagt tnnttcatct tcgtcttgat ccggctctgt ttgagcggcg acaggtattc gacgaacacg cggcccatca ggtgatccat ttcgtgctgg atgcatacgg cgagcaggcc ttcgcagtcg agctcgaacg tctcgccctg ctcgttgagc gcgcgcacgc gcacgcggtc cgggcgctcg acttcgtcgt agatgccggg caccgacaga cagccctctt cgtagacctg cttgccgtcg ctcgaccaga tgatctcggg gttgatgaag gcgcgcagct cgttcttgtc ttcggacacg tcgatcacga tcacgcgttc gtgcacgtcg acctgcgtcg cggcgaggcc gatgccgggc gccgcgtaca tcgtctcggc catgtcggca acgagcttgc ggatgcggtc gtcgaccttg tcgacgggct tcgcgacctt gtgcagccgc ttgtcggggt aatgaagaat gttcagcaaa gccatggtct ttcggtattc ggtgaagcgg tgtgccgcat tagccatatg gtggtggtgg tggtgagcca tggt
Expected Protein Sequence
MAHHHHHHMA NAAHRFTEYR KTMALLNILH YPDKRLHKVA KPVDKVDDRI RKLVADMAET MYAAPGIGLA ATQVDVHERV IVIDVSEDKN ELRAFINPEI IWSSDGKQVY EEGCLSVPGI YDEVERPDRV RVRALNEQGE TFELDCEGLL AVCIQHEMDH LMGRVFVEYL SPLKQSRIKT KMKKLERAM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta atgcggcaca ccgcttcacc gaataccgaa agaccatggc tttgctgaac attcttcatt accccgacaa gcggctgcac aaggtcgcga agcccgtcga caaggtcgac gaccgcatcc gcaagctcgt tgccgacatg gccgagacga tgtacgcggc gcccggcatc ggcctcgccg cgacgcaggt cgacgtgcac gaacgcgtga tcgtgatcga cgtgtccgaa gacaagaacg agctgcgcgc cttcatcaac cccgagatca tctggtcgag cgacggcaag caggtctacg aagagggctg tctgtcggtg cccggcatct acgacgaagt cgagcgcccg gaccgcgtgc gcgtgcgcgc gctcaacgag cagggcgaga cgttcgagct cgactgcgaa ggcctgctcg ccgtatgcat ccagcacgaa atggatcacc tgatgggccg cgtgttcgtc gaatacctgt cgccgctcaa acagagccgg atcaagacga agatgaagaa actcgaacgc gcgatgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t